• Title/Summary/Keyword: Maximum level

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Survey of the heterogeneous gene expression in olive flounder muscle using the luciferase reporter gene system

  • Hong, Suhee;Lee, Sang-Jun
    • Journal of fish pathology
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    • v.16 no.3
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    • pp.147-152
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    • 2003
  • The CMV promoter driven luciferase reporter gene coding plasmid (pcDNA-luc) was constructed and used as a model for DNA immunization study. Expression of the recombinant luciferase protein was confirmed in vitro in RTG-2 cell line before using in vivo study in olive flounder. In dose response study, the maximum expression of the luciferase gene was found in the group injected with 10-15μg of plasmid DNA. The kinetic study showed that the luciferase gene expression was reached at the maximum level at one day after injection and slightly decreased after then but significantly high level of expression was sustained until the conducted experiment of 7 days. In the study of tissue distribution of gene expression, it was found that luciferase gene was expressed at the significant level in immune organs such as gill and spleen, located far from the injected site, suggesting the systemic distribution of the intramuscularly injected DNA in olive flounder.

Glass FRP-Bonded RC Beams under Cyclic Loading

  • Tan, Kiang-Hwee;Saha, Mithun-Kumar
    • International Journal of Concrete Structures and Materials
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    • v.1 no.1
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    • pp.45-55
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    • 2007
  • Ten beams bonded with glass fiber reinforced polymer (GFRP) laminates were tested under cyclic loading with the load range and the FRP reinforcement ratio as test parameters. The maximum load level during cyclic loading was 55%, 65% and 75% of the static flexural strength while the minimum load level was kept constant at 35%. Deflections of the beams at the end of 525000 cycles were found to increase by 16% and 44% when the maximum load level was increased from 55% to 65% and 75% of the static flexural strength, respectively. Beams with FRP reinforcement ratios of 0.64% and 1.28% were found to exhibit lesser deflections of about 15% and 20%, respectively, compared to a similar beam without FRP reinforcement. An analytical approach based on cycle-dependent effective moduli of elasticity of concrete and FRP reinforcement is presented and found to predict the deflections of the test beams well.

A study on patterns of propagation for high speed train(KTX) (한국형 고속전철(KTX) 방사패턴에 관한 연구)

  • 구동회;김재철;박태원;문경호
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2001.05a
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    • pp.836-842
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    • 2001
  • The more sophisticated patterns of propagation model is presented in this paper, which includes three different source characteristics. The spherical, cosine and dipole radiation characteristics compared and sound event level and the maximum sound level are calculated by experiment and calculation. It is shown that patterns of propagation has dipole characteristics for low speed range(below about 150km/h) at electric multiple system. We know that push-pull high speed system(maximum speed: 300km/h) has cosine characteristics of noise propagation. For this purpose, We conduct the experiment of noise and know the empirical formula of noise level and radiation coefficient K. This model of simulation is conducted through point source array model at wheel/rail contact point by using program and experimental formula. We can guess prediction of profile, flat and wear of wheel by above modeling in near field.

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Quality Enhancement of Medical Images by Using Nonlinear Histogram Equalization Function (비선형 히스토그램 평활화 함수에 의한 의료영상의 화질개선)

  • Cho, Yong-Hyun
    • Journal of the Korean Society of Industry Convergence
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    • v.13 no.1
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    • pp.23-30
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    • 2010
  • This paper presents a histogram equalization based on the nonlinear transformation function for enhancing the quality of medical images. The nonlinear transformation function is applied to adaptively equalize the brightness of the image according to its intensity level frequency. The logistic function is used as a nonlinear transformation function, which is calculated by only using the intensity level with maximum frequency and the maximum intensity level in an histogram, and the total number of pixels. The proposed method has been applied for equalizing 8 medical images with a different resolution and histogram distribution. The experimental results show that the proposed method has the superior enhancement performances compared with the conventional histogram equalization. And the proposed histogram equalization can be used in various multimedia systems in real-time.

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ON THE DEVELOPMENT OF EXPLOSION TECHNOLOGY IN SEOUL METRO SUBWAY CONSTRUSTION (서울 지하철 건설의 발파기술 발전)

  • 許眞
    • Explosives and Blasting
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    • v.18 no.1
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    • pp.59-70
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    • 2000
  • The blasting work to construct a subway in seoul, korea have often cased increased neighbor's complaints because of ground vibration. In order to prevent the demage to the stucture it was necessary to predict the level of blasting induced vibration and to determine the maximum charge weigh per delay with an allowable vibration level. The effect of blasting pattem, rock strength and different explosive on the blast-induced ground vibration was studied to determine the maximum charage weight per delay within a given vibration level. The blasting vibration equation from over 100 test data was obtained, V= K(D/W(equation omitted), where the values for n and K are estimated to be 1.7 to 1.5 and 48 to 138 respectively.

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Sidelobe Level Optimization of Microstrip Patch Array using Genetic Algorithms (유전자 알고리즘을 이용한 마이크로스트립 패치 배열 안테나의 부엽레벨 최적화)

  • Kim, Dong-Hyun;Kim, Young-Sik
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 2003.11a
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    • pp.428-431
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    • 2003
  • In this paper, distances between elements are optimized for low sidelobe level (SLL) microstrip patch array using Genetic Algorithms. Genetic Algorithms are "global" numerical-optimization methods, it's advantages are very simple coding and fast optimization. This paper show how to optimize the maximum SLL using Genetic Algorithms. In the results, although mutual coupling is neglected, it's maximum SLL is 3.5 dB lower than Uniformly Spaced Array(distance=$0.5{\lambda}$).

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Volume Transport through the La-Perouse (Soya) Strait between the East Sea (Sea of Japan) and the Sea of Okhotsk

  • Saveliev Aleksandr Vladimirovich;Danchenkov Mikhail Alekseevich;Hong Gi-Hoon
    • Ocean and Polar Research
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    • v.24 no.2
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    • pp.147-152
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    • 2002
  • Seasonal and interannual variation of volume transport through the La-Perouse Strait were estimated using the difference of sea level observed at Krillion of Sakhalin, Russia, and Wakkanai of Hokkaido, Japan, during the period of 1975-1988. Historical sea level measurements between Russian and Japanese tide gauge data were normalized using an independent direct volume transport measurement. Volume transport from the East Sea (Sea of Japan) to the Sea of Okhotsk varied from -0.01 to 1.18 Sv with an annual mean value of 0.61 Sv. Monthly water transport rates showed a unimodal distribution with its maximum occurring in summer (August) and minimum in winter (December-February). The annual mean volume transport varied from 0.2 to 0.8 Sv during the period of 1975-1988 with the maximum variance of 0.6 Sv.

Seismic microzonation of Kolkata

  • Shiuly, Amit;Sahu, R.B.;Mandal, Saroj
    • Geomechanics and Engineering
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    • v.9 no.2
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    • pp.125-144
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    • 2015
  • This paper presents the probabilistic seismic microzonation of densely populated Kolkata city, situated on the world's largest delta island with very soft alluvial soil deposit. At first probabilistic seismic hazard analysis of Kolkata city was carried out at bedrock level and then ground motion amplification due to sedimentary deposit was computed using one dimensional (1D) wave propagation analysis SHAKE2000. Different maps like fundamental frequency, amplification at fundamental frequency, peak ground acceleration (PGA), peak ground velocity (PGV), peak ground displacement (PGD), maximum response spectral acceleration at different time period bands are developed for variety of end users, structural and geotechnical engineers, land use planners, emergency managers and awareness of general public. The probabilistically predicted PGA at bedrock level is 0.12 g for 50% exceedance in 50 years and maximum PGA at surface level it varies from 0.095 g to 0.18 g for same probability of exceedance. The scenario of simulated ground motion revealed that Kolkata city is very much prone to damage during earthquake.

Complementary DNA Cloning and Nucleotide Sequence Analysis of Coat Protein Gene from TMV Tomato Strain (토마토에서 분리된 담배 모자이크 바이러스 외피단백질 유전자의 cDNA 클로닝 및 염기서열 분석)

  • 이청호;이영기;강신웅;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.2
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    • pp.101-106
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    • 1996
  • Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.

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Link-Level Performance of Cooperative Multi-Hop Relaying Networks with MDS Codes

  • Sakakibara, Katsumi;Ito, Daichi;Taketsugu, Jumpei
    • Journal of Communications and Networks
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    • v.13 no.4
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    • pp.393-399
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    • 2011
  • We evaluate the link-level performance of cooperative multi-hop relaying networks with an maximum distance separable (MDS) code. The effect of the code on the link-level performance at the destination is investigated in terms of the outage probability and the spectral efficiency. Assuming a simple topology, we construct an absorbing Markov chain. Numerical results indicate that significant improvement can be achieved by incorporating an MDS code. MDS codes successfully facilitate recovery of the message block at a relaying node due to powerful error-correcting capability, so that it can reduce the outage probability. Furthermore, we evaluate the average number of hops where the message block can be delivered.