• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.18-27
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• 1997

The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.147-154
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• 2006

Effects of water temperature, salinity and irradiance on the growth of toxic dinoflagellate Alexandrium catenella (AlCt-01) were investigated, which was isolated from Jinhae Bay, Korea. The maximum growth of A. catenella strain was obtained at $150{\mu}E\;m^{-2}s^{-1}$. The ranges of temperature and salinity for the growth of the strain were $15\sim25^{\circ}C$ and $20\sim34$ PSU. The maximum growth rate of A. catenella determined was $0.45d^{-1}$ at $20^{\circ}C$, 30 PSU and $150{\mu}E\;m^{-2}s^{-1}$ We also examined the Se and Fe effects on the toxic dinoflagellate A. catenella in optimal growth conditions. As Fe concentration of medium increased from 0 to $11.7{\mu}M$, growth rate of A. catenella increased and the maximum growth rate(k=0.48 $d^{-1}$) was obtained at $11.7{\mu}M$ of Fe cone. Growth of A. catenella were stimulated at> 1nM of Se.

• Korean Journal of Food Science and Technology
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• v.37 no.6
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• pp.1012-1017
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• 2005

Predictive growth model of putrefactive bacteria of surimi-based imitation crab in the modified surimi-based imitation crab (MIC) broth was investigated. The growth curves of putrefactive bacteria were obtained by measuring cell number in MIC broth under different conditions (Initial cell number, $1.0{\times}10^2,\;1.0{\times}10^3$ and $1.0{\times}10^4$ colony forming unit (CFU)/mL; temperature, $15^{\circ}C,\;20^{\circ}C\;and\;25^{\circ}C$) and applied them to Gompertz model. The microbial growth indicators, maximum specific growth rate constant (k), lag time (LT) and generation time (GT), were calculated from Gompertz model. Maximum specific growth rate (k) of putrefactive bacteria was become fast with rising temperature and fastest at $25^{\circ}C$. LT and GT were become short with rising temperature and shortest at $25^{\circ}C$. There were not significant differences in k, LT and GT by initial cell number (p>0.05). Polynomial model, $k=-0.2160+0.0241T-0.0199A_0$, and square root model, $\sqrt{k}=0.02669$ (T-3.5689), were developed to express the combination effects of temperature and initial cell number, The relative coefficient of experimental k and predicted k of polynomial model was 0.87 from response surface model. The relative coefficient of experimental k and predicted k of square root model was 0.88. From above results, we found that the growth of putrefactive bacteria was mainly affected by temperature and the square root model was more credible than the polynomial model for the prediction of the growth of putrefactive bacteria.

• Microbiology and Biotechnology Letters
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• v.17 no.6
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• pp.619-623
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• 1989

The effects of temperature on yeast growth and ethanol production were investigated in batch cultures. The maximum specific growth rate of yeast was obtained at 36$^{\circ}C$ and the maximum specific production rate of ethanol was obtained at 33$^{\circ}C$. A mathematical model was employed to describe the temperature effects in ethanol fermentation and the parameters in the model were expressed as a function of temperature. Optimum temperature control strategy, from the simulation result, consists of starting the fermentation at high temperature and lowering the temperature as the fermentation proceeds.

• Journal of Korean Society for Quality Management
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• v.37 no.4
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• pp.16-22
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• 2009

The inflection S-shaped software reliability growth model (SRGM) proposed by Ohba(1984) is one of the most commonly used models and has been discussed by many authors. The main purpose of this paper is to estimate the parameters of Ohba's SRGM within the Bayesian framework by applying the Markov chain Monte Carlo techniques. While the maximum likelihood estimates for these parameters are well known, the Bayesian method for the inflection S-shaped SRGM have not been discussed in the literature. The proposed methods can be quite flexible depending on the choice of prior distributions for the parameters of interests. We also compare the Bayesian methods with the maximum likelihood method numerically based on the real data.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.2
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• pp.95-101
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• 2007

We investigated the growth and phosphate uptake of a toxic dinoflagellate, Gymnodinium catenatum, isolated from Yeosuhae Bay, South Korea. A short-term phosphate uptake experiment revealed that its maximum uptake and the half-saturation constant were 1.39 pmol/cell/hr and $2.65{\mu}M$, respectively. In a semicontinuous culture, the maximum specific growth rate and minimum phosphorus cell quota of G. catenatum were 0.39/day and 1.27 pmol/cell, respectively. Thus, G. catenatum is a poor competitor in terms of inorganic nutrient use and is unlikely to form blooms in Yeosuhae Bay.

• Microbiology and Biotechnology Letters
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• v.28 no.5
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• pp.285-290
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• 2000

The effects of dissolved oxy-gen(DO) concentration and DO control modes on cell growth in a 4L computer-controlled bioreactor system were investigated using Phellinus linteus WI-001, a producer of protein-polysaccharides having potent anticancer activi-ties. When DO was controlled at about 20%, maximum cell concentration and specific growth rate of the strain were observed to increase 36% and 64%, respectively, as compared to the experiment performed without DO control. By adopting cascade automatic control of DO ar 20% in a mixed automatic control mode using computer-con-trolled program, 19.5g/L of maximum cell concentration was obtained. These results showed that the mixed auto-matic control mode was the effective method for enhancing cell growth of the shear sensitive Phellinus linteus.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.457-462
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• 2002

Low Electromagnetic Field (EMF) intensity in the range of $1{\mu}T\;to\;10{\mu}T$(Tesla) was found to enhance the growth of CHO cells and the production of tPA in batch and perfusion cultivations. At $1{\mu}T\;intensity,\;1.3{\times}10^7$ viable cells/ml of maximum cell density and 80 mg/l of maximum tPA production were obtained in batch cultivation, compared to $2.8{\times}10^6$ viable cells/ml and 59 mg tPA/1 in unexposed case (control). A similar trend was observed in the perfusion process, where it was possible to obtain $1.2{\times}10^7$ viable cells/ml of maximum cell density and 81 mg tPA/l of maximum tPA production by more than 80 days of cultivation. However, there was not much difference between $1{\mu}T\;and\;10{\mu}T$ in perfusion cultivation, possibly due to better environmental growth conditions being maintained by continuous feeding of fresh medium into the reactor. On the contrary, both cell growth and tPA production were severely inhibited at higher than 1 mT intensity, showing no growth at 10 mT exposure. Specific growth rate was linearly correlated to specific tPA production rate at $1{\mu}T$EMF intensity, which represents a partially growth-related relationship. It was also found that a large amount of $Ca^2+$ was released at low EMF intensity, even though the cell growth was not much affected. Low EMF intensity significantly improved both cell growth and tPA production, and tPA production seemed to be more affected than the cell growth, possibly due to the changes of cell membrane characteristics. It can be concluded that the elaboration of EMF intensity less than $10{\mu}T$ could improve cell growth and tPA production, but mainly tPA secretion through batch or perfusion process in a bioreactor.

• Journal of Ocean Engineering and Technology
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• v.10 no.1
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• pp.53-64
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• 1996

In this paper, rotating bending fatigue tests have been carried out to investigate the growth behabiors of surface fatigue crack initiated from a small artificial surface defect, that might exist in real structures, on 2024-T3 and 6:4 brass. The test results are analysed in the viewpoints of both strength of materials and fracture mechanics, it can be concluded as follows. The effect of a small artificial surface defect upon the fatigue strength is very large. The sensitivity of 2024-T3 on the defect is higher than that of 6:4 brass. The growth behavior of the surface fatigue crack of 2024-T3 is different from that of 6:4 brass. The growth rate of the surface fatigue crack of 2024-T3 is considerably rapid in the early stage of the fatigue life and apt to decrease in the later stage. It was impossible to establish a unifying approach in the analysis of crack growth begabior of 2024-T3 and 6:4 brass using the maximum stress intensity factor because of their dependence on stress level. But if the elastic strain and cyclic total strain intensity factor range were applied to obtain the growth rate of surface fatigue cracks of the materials, the data were found to be nearly coincided.

• ALGAE
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• v.25 no.1
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• pp.17-26
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• 2010

The effects of salinity on various ecophysiological parameters of Ulva pertusa such as growth, nutrient uptake, photosynthetic performance and internal nutrient composition were tested. U. pertusa was collected from an eelgrass bed in a semi-protected embayment on the southwest coast of Korea. Under salinity regimes from 5 to 40 psu, the specific growth rates $(\mu)$ of U. pertusa ranged from 0.019 to $0.032\;d^{-1}$. Maximum growth rate was observed at 20 psu, and minimum at 40 psu. This species showed various uptake rates for nitrate and phosphate. Nutrient uptake was noticeably higher at intermediate salinity levels, and lower at both extremes. Salinity significantly influenced chlorophyll-$\alpha$ content and effective quantum yield. Tissue nitrogen content ranged from 1.5 to 2.9% N (dry weight), whereas tissue phosphorus ranged from 0.1 to 0.14% P (dry weight). The N : P ratio in the tissue of U. pertusa was considerably higher, ranging from 30 to 50. Increased growth at lower salinity suggests that the initial growth rate of U. pertusa is greater during the rainy season (i.e., late spring and early summer) than any other season during the year. The appearance of an Ulva bloom in eelgrass beds may be triggered by salinity more than by other environmental factors such as light and temperature.