• Korean Journal of Microbiology
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• v.35 no.4
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• pp.302-306
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• 1999

Production of biomass by fed-batch culture of Saccharomyces cerevisiae Hansen CBS5926, which is used to treat intestinal disorders, was investigated using ethanol as the sole carbon source. Ethanol was a better carbon source than glucose for high cell density culture of the st-rain since it could decrease the frequency of contamination while increasing the efficiency and final productivity of the fermentation process. Under optimal conditions, 38 g/ℓ of dry cell weight with $2.2{\times}10^{9}$ cfu/㎖ of maximum viable cell count was achieved after 72h cultivation. Freeze-drying of the cultured yeast cells resulted in severe reduction of viability. Of the freeze-drying protectants tested, 20% sucrose and 30% lactose were most effective for the preservation of yeast cells with a viability level of 16.3%. A combination of skim milk and lactose with 20% sucrose(w/v) exerted no synergistic influence upo the viability of the cells during cryopreservation by freeze-drying.

• Journal of Microbiology and Biotechnology
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• v.21 no.2
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• pp.204-211
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• 2011

To yield high concentrations of protein expressed by genetically modified Escherichia coli, it is important that the bacterial strains are cultivated to high cell density in industrial bioprocesses. Since the expressed target protein is mostly accumulated inside the E. coli cells, the cellular product formation can be directly correlated to the bacterial biomass concentration. The typical way to determine this concentration is to sample offline. Such manual sampling, however, wastes time and is not efficient for acquiring direct feedback to control a fedbatch fermentation. An E. coli K12-derived strain was cultivated to high cell density in a pressurized stirred bioreactor on a pilot scale, by detecting biomass concentration online using a capacitance probe. This E. coli strain was grown in pure minimal medium using two carbon sources (glucose and glycerol). By applying exponential feeding profiles corresponding to a constant specific growth rate, the E. coli culture grew under carbon-limited conditions to minimize overflow metabolites. A high linearity was found between capacitance and biomass concentration, whereby up to 85 g/L dry cell weight was measured. To validate the viability of the culture, the oxygen transfer rate (OTR) was determined online, yielding maximum values of 0.69 mol/l/h and 0.98mol/l/h by using glucose and glycerol as carbon sources, respectively. Consequently, online monitoring of biomass using a capacitance probe provides direct and fast information about the viable E. coli biomass generated under aerobic fermentation conditions at elevated headspace pressures.

• Geomechanics and Engineering
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• v.36 no.6
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• pp.589-599
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• 2024

Dispersion occurs when clay soil disperses under specific conditions and is rapidly washed away. While there are numerous methods for rectifying it, they are neither cost nor time-effective. The current study used metakaolin and zeolite to improve heavily dispersive clay soil either separately or in combination at 0%, 2%, 4%, 6%, and 8% of the soil weight. After 7 days of curing, the samples were tested to determine the extent of change in the dispersion potential, as well as the improvement of the geotechnical properties of the soil. The results indicated that the addition of 2% zeolite with 6% to 8% metakaolin decreased the dispersion potential considerably. Double hydrometry test findings revealed that the dispersion potential decreased by almost 70% and entered the non-dispersive group; the crumb test also revealed this. Atterberg limits testing indicated a decrease in the plasticity index which reduced the flexibility of the samples. The greatest decrease in PI (67.5%) was achieved with the addition of 8% zeolite plus 8% metakaolin to the soil. The results of density tests revealed that a decrease in the optimal moisture content increased the maximum dry density of soil. This increase in density was a response to the high reactivity of metakaolin with calcium hydroxide and the formation of calcium hydroxide hydrate gel. This eventually caused an increase in the unconfined compressive strength, the greatest increase in strength of about 1.8-fold was observed with a combination of 2% zeolite and 6% metakaolin compared to the unmodified sample.

• The Journal of Engineering Geology
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• v.32 no.1
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• pp.173-191
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• 2022

In this study, the shear wave velocity of the ground was measured using Flat TDR, and the precision analysis of the measured value and the verification of field applicability were performed. The shear wave velocity measurement value was derived in the field using the piezo-stack combined in the Flat TDR. analyzed. As a result of the experiment, the average value of the change in shear wave speed at the time of grout material injection was 10.15 m/s at the beginning of age, and the average value of the change in shear wave speed after the 7th to 14th days was 65.99 m/s, showing a tendency to increase with age. Also, it was found that dry density and shear wave speed increased as the water content increased on the dry side, and that the dry density and shear wave rate decreased as the water content increased on the wet side as the water content increased. The shear modulus value derived from the field test was confirmed to be a minimum of 17.36 MPa and a maximum of 28.13 MPa, confirming a measurement value similar to the reference value. Through this, it can be seen that the measured value of the shear modulus using Flat TDR is reliable data, and it can be determined that the compaction management of the site can be effectively managed in the future.

• Korean Journal of Agricultural Science
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• v.5 no.1
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• pp.15-28
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• 1978

It has been known that the shear strength of soil is an important design parameter for the foundation of structures, the retaining walls, the slope failures and so forth. In this study, the shear test was performed by using the direct shear apparatus under various degree of the moisture content and the density of the sample soils. The results of the study were summarized as follows; 1. The shear strength of soil increased with increase in the dry density of soil, and at the same level of density of the sample the shear strength of soil showed large values on a good grading of the sample. 2. The cohesion of the soil varied directly with the dry density of it, however the internal friction angle of soil was not affected by the dry density of tile sample. 3. The shear strength of sample varied inversly with the moisture content of it, and this phenomenon was apparent on a good grad ing of sample. 4. The cohesion of soil showed maximum value when the moisture content of the soil reached optimum level and the internal friction angle decreased with increase in the moisture content of it. These phenomena were very obvious on a good grading sample, SDC-1. 5. The cohesion of the soil decreased with increase in void ratio of the sample, but the internal friction angle of the sample didn't show such tendency.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.1
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• pp.89-99
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• 2006

The growth and biomass of reeds(Phragmites australis) growing in a subsurface treatment wetland system were investigated from April 2003 to October 2003. Nitrogen(N) and phosphorous(P) concentrations in above-ground(AG) and below-ground(BG) tissues of reeds were examined and the removal rate of N and P by reeds were analyzed. The system, 29 m in length, 9 m in width and 0.65 m in depth, was constructed in June 2001 on a floodplain in the down reach of the Kwangju Stream in Korea in order to purify polluted water of the stream. A bottom layer of 45 cm in depth was filled with crushed granites(15~30 mm in diameter) and a middle layer of 10 cm in depth was filled with pea pebbles(10 mm in diameter). An upper layer of 5 cm contained course sand. Reeds were transplanted on the surface of the system, which were dug out of natural wetlands, and their shoots were trimmed 40 cm in height. The height and density of the shoots averaged 237.7 cm and 244.0 shoot/$m^2$, respectively, when the reeds grew fully. The maximum biomass of AG and BG tissues were 1,964 and 1,577 g/$m^2$, respectively, and the AG : BG ratio of biomass was 1.26. Mean AG and BG dry weights were recorded as 1,355 and 748 g/$m^2$, respectively. The AG and BG tissue concentrations of N averaged 12.37 and 10.01 mg/g, respectively, and those of P 2.37 and 2.03 mg/g, respectively. Inflow to the system averaged 40 $m^3$/day. The concentrations of total nitrogen(T-N) in influent and effluent were 8.4 mg/L and 3.2 mg/L, respectively, and those of total phosphorous(T-P) were 0.73 and 0.38 mg/L, respectively. The total removal of T-N and T-P by the system during the investigation period averaged 140.2 and 9.7 g/$m^2$, respectively, and the total uptake of N and P by the reeds were calculated as 24.39 and 4.73 g/$m^2$, respectively. Average removals of about 17% of N and about 49% of P by reeds were recorded. The N and P concentrations in AG tissues were significantly different among the three zones of the system:near to inflow(St1), in the middle of system(St2), and near to outflow(St3). The N and P concentrations in BG tissues were also significantly different among St1, St2 and St3. N and P concentrations in AG and BG tissues of reeds growing in St1 were higher than those in St2 and St3. The height and density of shoots of reeds in St1 were larger than those in St2 and St3. Significant amounts of N and P in the influent were taken up by reeds in St1.

• Korean Journal of Agricultural Science
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• v.5 no.2
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• pp.49-55
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• 1978

In order to get healthy seedling of rice suitable for rice transplanter, the growing characteristics of the seedling grown under different growing days and sowing densities were invesigated and the results obtained were summarized as follows; In the 20 days old seedling, the difference of growing state of seed ling and its characteristics between the two types of nursery bed was not found, but for the 30 days old seedling, both of the crop growth rate and the relative growth rate on the band-type nursery bed appeared higher than those on the mat-type nursery bed. And also, "the dry weight, the content of cardohydrate and the ratio of dry weight to plant height of the seedling grown on the band-type nursery bed were much higher than those on the mat-type nursery bed, however the amount of difference of seedling characteristics between two types of nursery bed was decreased for 40 days old seedling and leaf number was not increased. The amount of the seedling growth was increased with less density of sowing and for the difference between the higher and the less density, the difference getting bigger with the longer nursery days. The maximum density of sowing was approximately 200cc per nursery case on the band-type nursery bed and 250cc per nursery case on the mat-type nursery bed. It was seemed that the growing uniformity of the seedling grown on the mat-type nursery bed was better than that on the band- type nursery bed, As the coefficient of variation of the plant height and the leaf numbers of the seedling grown on the band- type nursery bed was higher than that on the mat-type nursery bed.

• Korean Journal of Weed Science
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• v.3 no.1
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• pp.29-38
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• 1983

Growth habit, intraspecific competition of Digitaria sanguinalis, and interspecific competition between soybean and D. sanguinalis were evaluated to obtain the basic informations on establishing an effective control measure in upland corps. The earlier planting on April 1 produced approximately 3.5 times higher dry matter than that of the latest planting on July 22 and resulted in the earliest heading on July 8 and the latest heading was observed on September 4 planted on July. 22, but the interval between planting and heading dates was shortened progressively as the planting dates were delayed, showing irregularity of heading within a hill. Tiller numbers per plant were inhibited as densities increased from one to forty. One plant planted per pot produced significantly higher tiller numbers than density of 5 to 40 plants per pot. However, total tiller numbers was the highest in density of 40 plants planted per pot. Regardless of planting densities used, D. sanguinalis at all densities produced the similar dry matter per pot, showing severe intraspecific competition as density increased, but on each plant basis dry weight production was the highest in the lowest density such as one plant planted per pot. Competition between soybean and D. sanguinalis for the entire growing season decreased the total dry weight of soybean by 59.6%. Soybean required the maintenance of a weed free condition, about 3 to 4 weeks immediately after seeding, for obtaining the maximum yield, beyond which soybean crops effectively suppressed the growth of D. sanguinalis.

• Geotechnical Engineering
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• v.10 no.4
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• pp.83-102
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• 1994

For estimation of Ko value depending upon the stress history of dry sand, a new type of Ko oedometer apparatus is devised, and the horizontal earth pressure is accurately measured. For this study, 2 types of one-cyclic Ko loading/unloading models have been studied experimentally using four relative densities of the sand. The results obtained in this test are as follows Kon, the coefficient of earth pressure at rest for virgin loading is a function of the angle of internal friction of the sand and is determined as Kon=1-0.914 sin, Kou the coefficient of earth pressure at -rest for virgin unloading is a function of K. and overconsolidation ratio(OCR), and is determined as Kou : Kon(OCR)". The exponent u, increases as the relative density increases. Ko,, the coefficient of earth pressure at rest for virgin reloading decreases in hyperbola type as the vertical stress, cv', increases. And, the stress path at virgin reloading lends to the maximum prestress point, independent upon the value of the minimum unloading stress. The gradient of this curve, mr, increases as OCR increases.ases.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.11a
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• pp.67-67
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• 2009

Due to the scaling down of the dielectrics thickness, the leakage currents arising from electron tunneling through the dielectrics has become the major technical barrier. Thus, much works has focused on the development of high k dielectrics in both cases of memories and CMOS fields. Among the high-k materials, $Al_2O_3$ considered as good candidate has been attracting much attentions, which own some good properties as high dielectric constant k value (~9), a high bandgap (~2eV) and elevated crystallization temperature, etc. Due to the easy control of ion energy and flux, low ownership and simple structure of the inductively coupled plasma (ICP), we chose it for high-density plasma in our study. And the $BCl_3$ was included in the gas due to the effective extraction of oxygen in the form of BClxOy compound. In this study, the etch characteristic of ALD deposited $Al_2O_3$ thin film was investigated in $BCl_3/N_2$ plasma. The experiment were performed by comparing etch rates and selectivity of $Al_2O_3$ over $SiO_2$ as functions of the input plasma parameters such as gas mixing ratio, DC-bias voltage and RF power and process pressure. The maximum etch rate was obtained under 15 mTorr process perssure, 700 W RF power, $BCl_3$(6 sccm)/$N_2$(14 sccm) plasma, and the highest etch selectivity was 1.9. We used the x-ray photoelectron spectroscopy (XPS) to investigate the chemical reactions on the etched surface. The Auger electron spectroscopy (AES) was used for elemental analysis of etched surface.