• Title/Summary/Keyword: Maximum dilution

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Continuous Ethanol Production Using immobilized Baker's Yeast (고정화 효모를 이용한 연속적 에탄올 생산)

  • 한면수;하상도;정동효
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.398-404
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    • 1991
  • - Ethanol production by calcium alginate-immobilized baker's yeast was studied in the continuous shaked-flask reactor (CSFR) using glucose medium as a feed. Immobilized cells were stable at 30~$37^{\circ}C$ and pH 4~8. Fermentation characteristics of immobilized baker's yeast were examined changing the initial glucose concentration employed were 50, 100 and 150 g/l, respectively. It was investigated that the influent glucose concentration and the dilution rate have an influence on the ethanol fermentation characteristics at steady state in continuous culture of immobilized baker's yeast. The optimum conditions for high ethanol productivity and low residual glucose output in ethanol prodution were shown to be 0.2 h ' for the dilution rate and 150 g/l for the influent glucose concentration. The maximum ethanol productivity, ethanol yield, specific growth rate and glucose conversion rate were around 7.12 g/$l\cdot h$, 0.23, 0.366 g/$l\cdot h$ and 78.43, respectively.

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Dilution of Precision (DOP) Based Landmark Exclusion Method for Evaluating Integrity Risk of LiDAR-based Navigation Systems

  • Choi, Pil Hun;Lee, Jinsil;Lee, Jiyun
    • Journal of Positioning, Navigation, and Timing
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    • v.9 no.3
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    • pp.285-292
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    • 2020
  • This paper introduces a new computational efficient Dilution of Precision (DOP)-based landmark exclusion method while ensuring the safety of the LiDAR-based navigation system that uses an innovation-based Nearest-Neighbor (NN) Data Association (DA) process. The NN DA process finds a correct landmark association hypothesis among all potential landmark permutations using Kalman filter innovation vectors. This makes the computational load increases exponentially as the number of landmarks increases. In this paper, we thus exclude landmarks by introducing DOP that quantifies the geometric distribution of landmarks as a way to minimize the loss of integrity performance that can occur by reducing landmarks. The number of landmarks to be excluded is set as the maximum number that can satisfy the integrity risk requirement. For the verification of the method, we developed a simulator that can analyze integrity risk according to the landmark number and its geometric distribution. Based on the simulation, we analyzed the relationship between DOP and integrity risk of the DA process by excluding each landmark. The results showed a tendency to minimize the loss of integrity performance when excluding landmarks with poor DOP. The developed method opens the possibility of assuring the safety risk of the Lidar-based navigation system in real-time applications by reducing a substantial amount of computational load.

Insect Cell Cultures for Recombinant Protein Production (재조합 단백질 생산을 위한 곤충세포의 배양)

  • 박영민;정용주양재명정인식
    • KSBB Journal
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    • v.4 no.3
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    • pp.266-270
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    • 1989
  • Insect cell cultures were performed in laboratory-scale vessels. The batch growth of insect cells was affected by such parameters as serum content, other nutrients, seeding density, and mechanical agitation. Lactate and ammonium were not likely to be environmental factors that inhibited cell growth at the concentrations observed at the end of batch cultures. In addition, redox potential was found to be a useful index in monitoring low-level dissolved oxygen during the cultivation of insect cells. Recombinant protein production by cells infected with a genetically-modified baculovirus was also demons treated. The maximum beta-galactosidase synthesis of 2800 units per reactor volume was achieved at the dilution rate of $0.006hr^{-1}$.

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Emission Characteristics of Odorous Sulfur Gases from Food Types: A Case Study on Boiled Egg, Milk, Canned Meat, and Strawberry (음식물의 악취 황화합물 발생특성 조사: 계란, 우유, 고기통조림, 딸기에 대한 사례 연구)

  • Kim, Bo-Won;Ahn, Jeong-Hyeon;Kim, Ki-Hyun;Jo, Sang-Hee
    • Journal of Korean Society for Atmospheric Environment
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    • v.29 no.5
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    • pp.615-624
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    • 2013
  • In this study, the emission patterns of reduced sulfur compounds (RSC) were investigated using four different types of food samples (boiled egg, milk, canned meat and strawberry) between fresh and decaying stages. To this end, the concentrations of RSCs were measured at storage days of 0, 1, 3, 6, and 9 under room temperature. Four sulfur compounds ($H_2S$, $CH_3SH$, DMS and DMDS) were selected as target compounds along with two reference compounds ($CS_2$ and $SO_2$). Their concentrations were quantified using GC-PFPD equipped with thermal desorption (TD) system. The boiled egg showed the highest concentration of $H_2S$ (3,655 ppb) at D-1, while $CH_3SH$ reached its maximum value of 64.4~78.5 ppb after 3 days. In milk samples, concentration of $CH_3SH$, DMS, and DMDS went up to 487, 16.3, and 578 ppb, respectively with the progress of decay (D-9). In case of canned meat, concentration of $H_2S$ and $CH_3SH$ peaked in the beginning (D-0) such as 345 and 66.6 ppb. In case of strawberry, $CH_3SH$ and DMDS showed the maximum concentrations 135 and 50.5 ppb at D-1, respectively. The olfactometry dilution-to-threshold (D/T) ratio by air dilution sensory (ADS) test showed similar patterns when sum of odor intensity (SOI) was derived via conversion of odorant concentration data. The results of this study confirm that the time of strong RSC emissions is distinguished for each food type between fresh (e.g., strawberries) and decaying conditions (e.g., milk).

Pollutant Control using the Separation Wall between Stormwater and Sewage in a Combined Sewer System (우오수분리벽을 이용한 합류식 하수관거의 오염물질 제어효과)

  • Lee, Kuang Chun;Choi, Bong Choel;Lim, Bong Su
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.4
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    • pp.461-469
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    • 2004
  • This research is to determine the stormwater effects on sewer concentrations by measuring and comparing the flow and pollutant concentrations during dry and rainy periods in the existing BOX type combined sewer pipes. The monitoring was carried out in two sites, which are the Daesachen outfall having PE separation wall in BOX type combined sewer pipes and the Yongunchen outfall not having seperatioin wall. The average flow-weighted BOD concentraion in Yongunchen outfall is 2-fold lower than in Daesachen outfall because of the dilution effect from ravine water. However, the pollutant mass loading is 16 fold higher in Yongunchen outfall than in Daesachen outfall because of more flows. According to the research, the separation wall controls 52% pollutant mass during a storm period (11.5 mm/hr rainfall intensity). Therefore, the Yongunchen combined sewer system (CSS) need separation wall to control and to prevent more pollutant input in stream. In Daesachen area, the maximum sewer flow rate during a storm period measured about 10 fold bigger than average sewer flow during dry periods. Also the concentrations between rainy and dry periods increase approximately 33 fold for BOD and 120 fold for SS. In Yongunchen area, it increases about 9 fold for the maximum flow rate, 18 fold for BOD and 22 fold for SS during a storm. Therefore, the research is concluded that the separation wall between stromwater (or ravine water) and sewage can decrease the dilution effect in CSS and control the pollutant loading.

The Effect of Redox Potential on the Kinetics of Lysine Production by Corynebacterium glutamicum (Corynebacterium glutamicum에 의한 Lysine 생산에 있어서 산화환원 전위가 발효속도론적 특성에 미치는 영향)

  • 이진희;김성준;이재흥
    • Microbiology and Biotechnology Letters
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    • v.19 no.1
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    • pp.76-81
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    • 1991
  • - The effect of redox potential (ORP) on lysine production by a leucine auxotrophic regulatory mutant of Corynebacterium glutclmicum on molasses medium was investigated in a 2-1 jar fermentor at pH 6.9 and $32^{\circ}C$. At a dilution rate of D=O.l $h ^1$, a maximum yield of Yr,,s=0.24 was obtained in either carbon- or leucine-limited chemostat where the redox potential was between -60 mV and - 100 mV. This level of redox potential corresponded to moderate oxygen deficiency. Under a high oxygen deficient condition of the redox potential of - 130 rnV (oxygen-limited chemostat), all the kinetic parameters such as $Y_[p/s}, q_s\; and \; q_p$ were decreased significantly and significant amounts of byproducts including glycine, alanine and valine were accumulated in the culture, indicating that the control of redox potential is important in lysine fermentation. At the redox potential of - 40 mV, on the other hand, large quantities of arginine (up to 0.38g/l) and glutamic acid (up to 0.12 g/l) were produced. A maximum lysine productivity of 2.41 g/l/h was achieved at - 66 mV under a carbon-limited condition.

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Anaerobic codigestion of urban solid waste fresh leachate and domestic wastewaters: Biogas production potential and kinetic

  • Moujanni, Abd-essamad;Qarraey, Imane;Ouatmane, Aaziz
    • Environmental Engineering Research
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    • v.24 no.1
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    • pp.38-44
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    • 2019
  • The Biochemical Methane Potential (BMP) of fresh leachate and domestic wastewaters codigestion was determined by laboratory Bach Tests at $35^{\circ}C$ over a period of 90 d using a wide range of leachates volumetric ratios from 0% to 100%. To simulate wastewaters plant treatment step, all the ratios were first air stripped for 48 h before anaerobic incubation. The kinetic of biogas production was assessed using modified Gompertz model and exponential equation. The results obtained showed that cumulative biogas production was insignificant in the case of wastewaters monodigestion while the codigestion significantly improves the BMP. Air stripping pretreatment had positive effect on both ammonium concentration and volatiles fatty acids with reduction up to 75% and 42%, respectively. According to the Modified Gompertz model, the optimal anaerobic co-digestion conditions both in terms of maximal biogas potential, start-up period and maximum daily biogas production rate, could be achieved within large leachate volumetric ratios from 25% to 75% with a maximum BMP value of 438.42 mL/g volatile solid at 50% leachate ratio. The positive effect of codigestion was attributed to a dilution effect of chemical oxygen demand and volatile fatty acid concentrations to optimal range that was between 11.7 to $32.3gO_2/L$ and 2.1 to 7.4 g/L, respectively. These results suggested that the treatment of fresh leachate by their dilution and co digestion at wastewaters treatment plants could be a promising alternative for both energetic and treatment purposes.

Temperature Profile Analysis of $Ti5_Si_3$ in Self-Propagating High Temperature Synthesis (SHS 법에 의한 $Ti5_Si_3$의 합성시 온도 Profile 분석)

  • 김도경;이형직;김익진;이형복
    • Journal of the Korean Ceramic Society
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    • v.32 no.3
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    • pp.341-348
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    • 1995
  • An analysis of the use of temperature profiles in the determination of the kinetic parameters of combustion synthesis of Ti5Si3 were investigated. From profile analysis, an apparent activation energy of 12KJ/mol was calculated. The Maximum heating rate achieved during 10wt% Ti5Si3 reaction by the product dilution method was approximately $1.5\times$104 K/s. Coupling this value with the measured wave velocity of 7.02 cm/s yields a maximum thermal gradient of 2.14$\times$103 K/cm. The value of tr (=t*) was calculated to be 1.2$\times$10-1 s and the value of td (=tx) was calculated to be 32.89 s. Using the definition of t* and the measured wave velocity, the effective thermal diffusivity, $\alpha$, was calculated to be 0.59$\times$10 $\textrm{cm}^2$/s. From these analysis, the power function, G, was also calculated.

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Synthesis and Characterization of New Orange-Red Light-Emitting PPV Derivatives With Bulky Cyclohexyl Groups

  • Ko, Seung-Won;Jung, Byung-Jun;Cho, Nam-Sung;Shim, Hong-Ku
    • Bulletin of the Korean Chemical Society
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    • v.23 no.9
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    • pp.1235-1267
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    • 2002
  • A series of 2,5-dialkoxy substituted poly(1,4-phenylenevinylene) derivatives containing a rigid and bulky cyclohexyl group in the side chain, poly[2-(7-cyclohexylheptyloxy)-5-butoxy-1,4-phenylenevinylene] (PBCyHpPV), Poly[2-(6-cyclohexylmethoxyhexyloxy)-5-butoxy-1,4-phenylenevinylene] (PBCyHxPV), Poly[2,5-di-(6-cyclohexylmethoxy-hexyloxy)-1,4-phenylenevinylene] (PDCyHxPV) were synthesized via the Gilch polymerization. The synthesized polymers were soluble in common organic solvents and showed good thermal stability up to $370^{\circ}C$. The maximum absorption of PBCyHpPV, PBCyHxPV and PDCyHxPV as thin films was at 513 ㎚, 515 ㎚, 511 ㎚, respectively. Photoluminescence maximum emission of above polymers appeared at 590 ㎚, 597 ㎚, 590 ㎚, respectively. The electroluminescence (EL) maxima of the polymers appeared around 585-590 ㎚, and also showed another shoulder around 630 ㎚ strongly. PDCyHxPV showed the highest EL efficiency and EL power than those of other polymers due to the dilution effect of the two rigid and bulky cyclohexyl groups.

Partial molal volumes of tetraalkylammonium chlorides in isopropanol-water mixtures (2-프로판올-물 혼합용매에서의 Tetraalkylammonium Chlorides의 분몰랄 부피)

  • Byung-Rin Cho
    • Journal of the Korean Chemical Society
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    • v.15 no.2
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    • pp.95-105
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    • 1971
  • The apparent molal volumes ${\phi}_v$ of a series of homologous tetraalkylammonium chlorides, $Me_4NCl, Et_4NCl, Pr_4NCl,\;and\;Bu_4NCl$, in a series of isopropanol-water mixtures have been determined at $30^{\circ}C$ from precision density measurements using pycnometers. The values of ${\phi}_v$ extrapolated to infinite dilution give partial molal volumes $\={V}^{\circ}$s which are almost additive for successive homologues and enable one to determine the absolute individual ionic partial molal volumes. The experimental results are discussed in terrns of the varying size and surface charge of the ions and the structure of the binary solvent mixtures. The results indicate that at 0.1 mole fraction isopropanol the maximum structuredness of water causes a minimum in the tetraalkylammonium-cation partial molal volumes, while at 0.2 mole fraction the solvent structure is such that the free volumes is a minimum and the electrostrictive effect of chloride ion is a maximum.

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