• Title/Summary/Keyword: Maximum dilution

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A Comparison Study of Signal Intensity of Gadolinium Contrast Media on Fast Spin echo and Ultra Short Time Echo Pulse Sequence at 3T MRI-Phantom Study (3T 자기공명영상 Fast Spin Echo (FSE)와 Ultra Short Time Echo (UTE) 펄스 시퀀스에서 가돌리늄 조영제 희석농도와 신호강도 비교 -팬텀 연구)

  • Lee, Suk-Jun;Yu, Seung-Man
    • Journal of radiological science and technology
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    • v.38 no.3
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    • pp.253-259
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    • 2015
  • The information of contrast media concentration on target organ is very important to get reduce the side effect and high contrast imaging. We investigated alternation of signal intensity as a function of the modality of Gd-based contrast media on spin echo and ultra short time echo (UTE) of T1 effective pulse sequence at 3T MRI unit. Gadoxetic acid, which is a MRI T1 contrast medium, was used to manufacture an agarose phantom diluted in various molarities, and sterile water and agarose 2% were used as the buffer solution for the dilution. The gold standard T1 calculation was based on coronal single section imaging of the phantom mid-point with 2D Inversion recovery spine-echo pulse sequence MR imaging for testing of phantom accuracy. The 1-2mmol/L and 7mmol/L was shown the maximum signal intensity on spin echo and UTE respectively. We confirm the difference of contrast media concentration which was shown the maximum signal intensity depending on the T1 effective pulse sequence.

Characterization of canine coronaviruses isolated from the dogs with diarrhea in Korea (설사증 이환견(犬)으로 부터 분리(分離)한 canine coronavirus의 성상(性狀)에 관한 연구(硏究))

  • Lee, Byung-hyung;Jun, Moo-hyung;Park, Jong-hyeon;Hwang, Eui-kyung;Huh, Won
    • Korean Journal of Veterinary Research
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    • v.34 no.3
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    • pp.517-527
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    • 1994
  • An attempt was made to isolate a causative viral agents from the fecal specimens of the diseased dogs with the gastroenteritis symptoms. Two coronavirus-like agents were isolated by serial dilution end point method and plaque assay. The isolates were characterized in terms of cytopathology, antigenicity, replication, physicochemical and morphological properties. The results obtained through the experiment were as follows; 1. Among 7 fecal specimens collected from the dogs with enteric disease, 2(28.6%)coronavirus-like agents showing typical cytopathic effects of canine coronavirus were isolated, and designated as CCV D1 and CCV D2, respectively. 2. By the cross-neutralization test and indirect immunofluoresence antibody test, the isolates were antigenically indentified as the standard CCV. The viruses were replicated only in the cytoplasm of A-72 cells. 3. The isolates showed no haemagglutinating activity against the erythrocytes from 11 kinds of animals. 4. The electron microscopic observation for the isolates showed spherical and pleomorphic features, covered with club-shaped projections on the surface. The size of particles was ranged from 70 to 150nm. 5. In one-step growth curve for the isolates in A-72 cells, maximum titers of intracellular vius was $10^{4.6}$ $TCID_{50}/0.1ml$ at 46 hrs postinoculation(pi) of CCV Dl and $10^{4.4}$ $TCID_{50}/0.1ml$ at 34 hrs pi of CCV D2. The maximum titers of extracellular virus was $10^{5.5}$ $TCID_{50}/0.1ml$ at 58 hrs pi of CCV D1 and $10^{5.8}$ $TCID_{50}/0.1ml$ at 46 hrs pi of CCV D2. 6. In physicochemical property test, the isolates were very sensitive to choroform and were found to be RNA virus. The viruses was stable at pH 3.0 for 1 hr and at $22{^{\circ}C}$ for 5 hrs. However, infectivity titers reduced remarkably by treatment with $56{^{\circ}C}$ for 10min.

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A numerical study of the air fuel ratio effect on the combustion characteristics in a MILD combustor (공연비 변화가 MILD 연소 특성에 미치는 영향에 관한 해석적 연구)

  • Ha, Ji-Soo;Kim, Tae-Kwon;Shim, Sung-Hoon
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.6
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    • pp.587-592
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    • 2010
  • A numerical analysis of reactive flow in a MILD(Moderate and Intense Low oxygen Dilution) combustor is accomplished to elucidate the characteristics of combustion phenomena in the furnace with the variation of air fuel ratio. For the smaller magnitude of air injection velocity(10 m/s), the air flow could not penetrate toward upper part of furnace. On the other hand, the air flow suppresses the fuel flow for the case of air injection velocity 30 m/s. The air velocity 18 m/s is corresponding to the stoichiometric air flow velocity, and for that case, the air flows to relatively more upper part of the furnace when compared with the case of air injection velocity 10 m/s. The reaction zone is produced with the previous flow pattern, so that the reaction zone of the air injection velocity 10 m/s is biased to the air nozzle side and for the case of air injection velocity 30 m/s, the reaction zone is inclined to the fuel nozzle side. For the cases with the air injection velocities 16, 18, 20 m/s, the reaction zone is nearly located at the center between air nozzle and fuel nozzle. The maximum temperatures and NOx concentrations for the cases of air injection velocity 16, 18, 20 m/s are lower than the cases with air injection velocity 10, 30 m/s. From the present study, the stoichiometric air fuel ratio is considered as the most optimal operating condition for the NOx reduction.

Characteristics for Co-digestion of Food Waste and Night Soil using BMP Test (BMP실험을 이용한 음식물폐기물 및 분뇨의 병합소화 특성)

  • Cho, Jinkyu;Kim, Hyungjin;Oh, Daemin
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.9
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    • pp.13-18
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    • 2014
  • BMP test was carried out to evaluate the characteristics for co-digestion of night soil and food waste. 6 types of sludge were tested in 30 days which were raw, excess, digested, night soil/septic tank (1:1), food waste (food : dilution water = 1:1), and mixed sludge. Bio gas was produced actively after 2 days, and continued in 2 weeks. Gas generation amount was decreased rapidly after considerable space of time. Especially maximum productivity of gas was shown in 7~8 days. The ultimate methane yields of raw, excess, digested, night soil/septic tank, food waste, and mixed sludge were 64.63, 67.49, 66.45, 72.44, 107.85, and 46.71 mL $CH_4/g$ VS respectively from Modified Gompertz model. The lag growth phase time and maximum specific methane production rate of mixed sludge were 1.88 day and 80.4 mL/day respectively. The methane potential of mixed sludge was higher than individual sludge. So high methane potential was expected by controlling mixing ratio of food waste. Besides stable operation of digestion tank and the solution of oligotrophic problem were possible.

A Numerical Study of the Combustion Characteristics in a MILD Combustor with the Change of the Fuel and Air Nozzle Position and Air Mass Flow Rate (연료 및 공기 노즐 위치와 공기 유량 변화에 따른 MILD 연소 특성에 관한 해석적 연구)

  • Kim, Tae-Kwon;Shim, Sung-Hoon;Chang, Huyk-Sang;Ha, Ji-Soo
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.5
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    • pp.325-331
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    • 2011
  • A numerical analysis of reactive flow in a MILD(Moderate and Intense Low oxygen Dilution) combustor is accomplished to elucidate the characteristics of combustion phenomena in the furnace with the change of fuel and air nozzle position and air mass flow rate. For the case with the fuel nozzle located near center position of combustor, the reaction zone started at the fuel nozzle and had inclined shape toward combustor wall when the air mass flow rate was relatively smaller. On the other hand, the end of reaction zone moved toward center of combustor from combustor wall when the air flow rate was relatively larger. For the case with the air nozzle located near center position of combustor, the reaction zone started at the fuel nozzle and had inclined shape toward combustor wall when the air mass flow rate was relatively small, which was similar as the previous case with smaller air mass flow rate. On the other hand, the end of reaction zone moved toward combustor wall when the air flow rate was relatively larger. The maximum temperature increased as the air mass flow rate increasing for both cases, and the concentration of thermal NOx increased also from the previous reason of temperature characteristics. The concentration of NOx for the case with the air nozzle located near center position of combustor was considerably smaller than that for the case with the fuel nozzle located near center position of combustor. From the present study, the case with the air nozzle located near center position of combustor and theoretical air flow rate was the most effective condition for the NOx reduction and perfect combustion.

Production of Organic Acids from Food By-Products - Mass Production of Organic Acids by Continuous Flow Ceil Recycling Fermentation - (식품부산물로부터 유기산의 대량생산공정에 관한 연구 - 세포재순환식 연속발효를 이용한 유기산의 대량 생산 -)

  • Ju Yun-Sang;Jin Sun-Ja;Hwang Pil-Gi;Choi Chul-Ho;Lee Eui-Sang
    • KSBB Journal
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    • v.19 no.6 s.89
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    • pp.484-488
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    • 2004
  • Fermentation studies were conducted in batch and continuous flow cell-recycle reactors with food by-products as substrates. The genus Propionibacterium acidipropionici ATCC 4965 was utilized in the production of organic acids. Good performance was achieved in the batch fermentation using hydrol as a carbon source and corn steep liquor (CSL) as nitrogen and vitamin sources. Product yields and productivity based on maximum values were 0.80 g total acids/g glucose and 0.26 g total acids/L/h, respectively, when $3\%$, (w/v) of hydrol and $2.5\%$, (w/v) of CSL were utilized. Continuous fermentation with cell-recycling system using the optimum amounts of substrates resulted in dramatic increase in cell concentration (X) and maximum productivity (P). Compared to the batch fermentation, X and P were increased by as much as 21 and 13 times, respectively, at the dilution ratio of $0.2\;hr^{-1}$, indicating that cell recycling fermentation of food by-products provides valuable means for the mass production of organic acids as well as utilizing cell mass as good nutrient resources.

Spatial and Temporal Distribution of Fish Communities with Rainfall in Jungrang Stream (강우에 따른 중랑천 어류군집의 시공간적 분포 특성)

  • Lee, Seung-Hyun;Jeong, Hyun-Gi;Shin, Hyun-Seon;Kim, Jin-young;Pak, Sangsuk;Shin, Yuna;Moon, Jeong-Suk;Lee, Su-Woong;Lee, Jae-Kwan
    • Korean Journal of Ecology and Environment
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    • v.51 no.4
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    • pp.354-364
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    • 2018
  • In this study, we surveyed the fish community at the four sites(St. 1, 3, 5, 7) in Jungrang Stream from 2008 to 2016. We found 37 species grouped into nine families. There were three exotic species; Cyprinus carpio, Carassius cuvieri and Micropterus salmoides after rainfall. Dominant species was Zacco platypus (57.3%) and subdominant species was Carassius auratus(10.4%) in a community. The water quality, surveyed at the six sites(St. 1, 2, 3, 4, 5, 6), based on eight factors(Water temperature, pH, DO, BOD, SS, EC, TN and TP), largely varied depending on each site and period. Minimum values in four factors(BOD, EC, TN and TP) were observed in rainy season, indicating an ionic and nutrient dilution of stream water by precipitation. In contrast, a maximum value in SS was occurred in rainy season at St. 2 and St. 5. The precipitation, Maximum value observed in July ($497.5{\pm}297.2mm$), minimum value in January ($12.9{\pm}8.6mm$). In July and August, the precipitation was divided into before and after the rainfall season in Jungrang stream. Using cluster analysis three fish sites (St. 1, 3, 5) were identified as significantly influence 11 fish species; Hemiculter eigenmanni, Squalidus japonicus coreanus, Hemibarbus labeo, Gnathopogon strigatus, Pungtungia herzi, Rhynchocypris oxycephalus, Pseudogobio esocinus, Pseudorasbora parva, Cyprinus carpio, Carassius auratus and Zacco platypus by rainfall.

Pytotoxicity by Continuous Spraying of Fruit Fire Blight Disinfectant During Growing Season of Apple and Pear (과수 화상병 방제약제의 사과·배 생육기 연용 살포에 의한 약해)

  • Se Hee Kim;Song-Hee Ryu;Byeonghyeon Yun;Kang Hee Cho;Sang-Yun Cho;Jung Gwan Park
    • Korean Journal of Plant Resources
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    • v.36 no.1
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    • pp.100-106
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    • 2023
  • In order to control the fire blight disease, all plants within the radius of the diseased orchard were removed in the early stage of the outbreak, or antibiotics control was performed for prevention. Since the beginning of antibiotics use on plants, the potential for development of resistance to antibiotics by the plant pathogen and unintended detrimental effects on the fruit trees and environment has become a problem. The purpose of this study is to determine the degree of phytotoxicity to fruit trees caused by excessive spraying of the fire blight disease disinfectant and to establish basic data for safe disinfectant guide. We analyzed whether damage to the fruit tree and the maximum residual limit of fruit was exceeded when three kinds of the fire blight disease disinfectants were continuously sprayed in excess of the number of safe use during the growing season. There was no phytotoxicity in apple 'Fuji' and pear 'Niitaka', and oxolinic acid was detected beyond the limit of quantitation in 'Fuji' grown without a bag, and the other disinfectants were detected below the maximum residue limit. When these disinfectants are continuously sprayed in excess of the number of safe, phytotoxicity may remain on the fruit. Therefore, it is necessary to observe the prescribed dilution factor and observe the safe frequency and the timing of use.

Antimicrobial Susceptibility of Enterococcus spp. Isolated from Han-river Area in Korea (한강 유역에서 분리한 Enterococcus 속 세균의 항생제 감수성)

  • Kim, Mal-Nam;Kwon, Oh-Min
    • Korean Journal of Environmental Biology
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    • v.26 no.3
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    • pp.240-246
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    • 2008
  • From the 4 sampling stations located in the basin of the Han River, total 46 strains of Enterococcus spp. composed of 15 E. faecium strains, 26 E. casseliflavus strains, 1 E. faecalis strain and 4 E. hirae strains were isolated. Among the 46 strains, 45 strains exhibited streptomycin-resistance, while 21 and 19 stains were resistant against tetracycline and quinupristin/dalfopristin, respectively. As for gentamicin and vancomycin, 15 strains and 1 strain showed resistance against the respective antimicrobial agents. Among the 46 strains, 39 strains showed resistance against more than 2 antimicrobial agents, and 10 strains demonstrated resistance to more than 5 antimicrobial agents. Especially, the strain isolated from the station C at Anyangcheon, exhibited resistance against all the 8 kinds of the antimicrobial agents. As the sampling site approached to the lower stream of the Han-river, the antibiotic resistant strains and the multi-drug resistant strains were detected more frequently. The MIC values of the antibiotic resistant strains measured by the disc diffusion method disclosed that 16 strains possessed maximum MIC value of 4,096 ${\mu}g$ mL$^{-1}$ against streptomycin and 17 strains possessed maximum MIC value of 2,048 ${\mu}g$ mL$^{-1}$ against gentamicin. Meanwhile, 1 strain exhibited maximum MIC value of 5121 ${\mu}g$ mL$^{-1}$ against vancomycin. As for quinupristin/dalfopristin and tetracycline, 2 and 33 strains showed maximum MIC value of 641 ${\mu}g$ mL$^{-1}$, respectively. Comparison of the MIC values of the strains of the this study with those of the strains of the other research groups isolated from the hospital drainage and also those from the live stock farm drainage indicated that the strains resistant against vancomycin and quinupristin/dalfopristin may be originated from the livestock farm drainage.

Spatio-temporal Variation Analysis of Physico-chemical Water Quality in the Yeongsan-River Watershed (영산강 수계의 이화학적 수질에 관한 시공간적 변이 분석)

  • Kang, Sun-Ah;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.39 no.1 s.115
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    • pp.73-84
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    • 2006
  • The objective of this study was to analyze long-term temporal trends of water chemistry and spatial heterogeneity for 10 sampling sites of the Yeongsan River watershed using water quality dataset during 1995 to 2004 (obtained from the Ministry of Environment, Korea). The water quality, based on multi-parameters of biological oxygen demand (BOD), chemical oxygen demand (COD), conductivity, dissolved oxygen (Do), total phosphorus (TP), total nitrogen (TN) and total suspended solids (TSS), largely varied depending on the sampling sites, seasons and years. Largest seasonal variabilities in most parameters occurred during the two months of July to August and these were closely associated with large spate of summmer monsoon rain. Conductivity, used as a key indicator for a ionic dilution during rainy season, and nutrients of TN and TP had an inverse function of precipitation (absolute r values> 0.32, P< 0.01, n= 119), whereas BOD and COD had no significant relations(P> 0.05, n= 119) with rainfall. Minimum values in conductivity, TN, and TP were observed during the summer monsoon, indicating an ionic and nutrient dilution of river water by the rainwater. In contrast, major inputs of total suspended solids (TSS) occurred during the period of summer monsoon. BOD values varied with seasons and the values was closely associated (r=0.592: P< 0.01) with COD, while variations of TN were had high correlations (r=0.529 : P< 0.01) with TP. Seasonal fluctuations of DO showed that maximum values were in the cold winter season and minimum values were in the summer seasons, indicating an inverse relation with water temperature. The spatial trend analyses of TP, TN, BOD, COD and TSS, except for conductivity, showed that the values were greater in the mid-river reach than in the headwater and down-river reaches. Conductivity was greater in the down-river sites than any other sites. Overall data of BOD, COD, and nutrients (TN, TP) showed that water quality was worst in the Site 4, compared to those of others sites. This was due to continuous effluents from the wastewater treatment plants within the urban area of Gwangju city. Based on the overall dataset, efficient water quality management is required in the urban area for better water quality.