• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.189-195
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• 1996

In order to obtain basic information for breeding varieties of onion which is suitable at the southern part of Korea. 222 early mature variety and 219 late mature variety were grown from sep.1993 to jun. 1994 at experimental field of Muan-Gun area, and yield components and yield of onion plant ware observed. Early mature onion cv. Changnyeongchunghwan-gkeukjoseng and Keumjungjoseng showed higher yield than other early mature variety used in the experiment. It showed relatively long plant height and leaf sheath length and, height of bulb and diameter of bulb and high weight of bulb. Late mature onion cv. Chunjudaego showed higher yield than other late mature variety used in the experiment. It showed relatively long plant height and leaf sheath length and diameter of bulb, and high weight of bulb. Therefore, it was concluded that Changnyeongchunghwangkeukjoseng,Keumjungjoseng and Chunjudaego were the most suitable early and late mature varieties with high yield at the southern part of Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.3
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• pp.209-214
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• 1988

This experiment was carried out to determine the effect of nitrogen rate and maturity on the yield, quality, nitrogen compound, protein pattern, and nitrate reductase activity. The results were as follow. As the nitrogen rate increased, the degree of red color of cured leaf increased. According to the time of harvesting at their leaf position, the price of the lugs is lowest in immature stage compared with mature and over mature stage but the upper leaves were lower than mature and immature stage in price. Yield are equal in immature stage and mature stage but over mature stage is lower than mature and immture stage. There was no different protein pattern and nitrate reductase activity in nitrogen rate and mature stage.

• Applied Microscopy
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• v.31 no.4
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• pp.367-374
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• 2001

Leaves of two developmental stages of Salsola species, young and mature, were examined to reveal the structural and functional relationships in the photosynthetic tissue using anatomical and ultrastructural criteria. Both young and mature leaves had Kranz anatomy of the Salsolid type with two layers of chlorenchyma on the leaf periphery: an outer layer of palisade mesophyll cells and an inner layer of compact bundle sheath cells with centripetally arranged organelles. The chlorenchyma was continuous in young leaves , while it was discontinuous in mature leaves. The main vascular bundle occupied the central position in the leaf. but the small peripheral vascular bundles were in contact with the chlorenchyma. Structural dimorphism of chloroplasts was obvious in bundle sheath cells of mature leaves exhibiting noticeable grana reduction, whereas mesophyll cell chloroplasts had well developed grana in all cases. Plasmodesmata were less numerous and rather simple in young leaves relative to well-developed secondary plasmodesmata of the later stage. According to the current data, features of two stages of Salsola leaves corresponded to NADP-ME bio-chemical subtype on the basis of photosynthetic cell ultrastructure. Implications of developing such anatomical and ultrastructural data of Sulsola species and biochemical characteristics reported in other C-4 species have been discussed.

• Journal of Ginseng Research
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• v.35 no.2
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• pp.155-161
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• 2011

Leaf characteristics of mature 2, 3 and 4-year-old North American ginseng (Panax quinquefolius L.) leaves on fruiting and non-fruiting(NF) plants were studied. Leaflets of the 2-year-old plants had the lowest fresh and dry weight, area, volume and internal gas volume. Inflorescence removal in 3-year-old plants did not affect leaf characteristics or ginsenoside concentration but in 4-year-old plants it increased leaf fresh (38.6%) and dry (43.9%) weight, leaf area (29.1%), specific leaf mass (11.4%), leaf volume (43.1%), and leaf thickness (12.1%), and decreased leaf water content (6.2%). Cultivated ginseng, although an understorey plant, had the specific leaf mass, 35.6 g $m^{-2}$ (range, 36 to 39 g $m^{-2}$) and a chlorophyll a/b ratio of 2.40 to 2.61, both suggesting the ability to perform like a sunny habitat plant. Also, specific leaf mass of 35.6 g $m^{-2}$ is similar to that reported for perennial plants, 36.8 g $m^{-2}$, rather than that for annuals, 30.9 g $m^{-2}$.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.48-49
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• 2003

This project was conducted to development several camellia tea mixed herb teas having any physiological effects. Leaves of tea tree contain many compounds, such as polysaccharides, volatile oils, vitamins, minerals, purines, alkaloids(eg. caffeine) and polyphenols(catechins and flavonoids). Although all three tea types(green, oolonr and black) have antibacterial and free radical capturing(antioxidizing) activities, the efficacy decreases substantially the darker the variety of tea is. This is due to lower contents of anti-oxidizing polyphenols remaining in the leaves. Unlike tea tree(Camellia sinensis), the biochemical features and effects of camellia(Camellia japonica) are not well known. Fresh mature leaf of sasanqua camellia(C. sasanqua), roasted young leaf tea(C. japonica) and fresh mature leaf and bark of camellia had high antibacterial activity against P. vulgaris and B. subtilis. In antifungal activity bioassay, young leaf roasted teas of camellia and sasanqua camellia had high activity against C. albicans and T. beigelil. Plant extracts from Camelia japonica had higher inhibitory activity against fungi than against bacteria. In cytotoxic effect against human acute myelogenous leukaemia cell extracts including fresh leaf(200$\mu\textrm{g}$/m1), bark(230$\mu\textrm{g}$/ml) and flower tea (320$\mu\textrm{g}$/m1)inhibited growth of AML cells.(중략)

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.321-328
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• 2010

An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g $1^{-1}$ sucrose, 2.2 g $1^{-1}$ Gelrite, and 7.7 lM naphthalene acetic acid (NAA) with 2.2 ${\mu}M$ thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 ${\mu}M$ TDZ, 2.2 ${\mu}M$ NAA, and 3.3 ${\mu}M$ silver nitrate ($AgNO_3$). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 ${\mu}M$ gibberellic acid ($GA_3$). The elongated shoots were rooted in MS medium supplemented with 4.0 ${\mu}M$ indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.297-302
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• 2019

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus 'Nanpa' using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus 'Nanpa' can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus 'Nanpa' in the future.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.273-278
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• 1998

Carbohydrate metabolism and partitioning are dependent on relationships between sources and sinks which can be affected by rates of photosynthesis and respiration. Fructan, the major form of stored carbohydrate in tall fescue (festuca arundineacea Schreb.), changes in concentration during growth and in response to the environment. Objectives of this study were i) to examine the content and the composition of carbohydrates in five tissues (mature leaf blade, immature leaf blade, leaf elongation zone, terminal meristem, and root tips) of two tall fescue genotypes, one with high yield per tiller (HYT) and one with low yield per tiller (LYT), and ii) to compare the reserved and utilized carbohydrates among above five different tissues, particularly between the leaf elongation zone and root tips. The established vegetative tillers of the HYT and LYT genotypes were grown in a controlled-environment growth chamber. Water-soluble carbohydrate (WSC) in the leaf elongation zone was about 22% of dry weight in the HYT and about 19% in the LYT genotype. The root tip also had high WSC, about 12% of dry weight in the HYT and 6% in the LYT genotype. Hexoses and sucrose were the major components of total WSC in all tissues except the leaf elongation zone. The growing tissues (sinks), i.e., the leaf elongation zone and root tip, had a high proportion of low degree of polymerization fructan, i.e., 3 to 8 hexose units.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.1-5
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• 1982

The metabolic enzymes, nitrate reductase, amylase and peroxidase and the Photorespiratory enzyme, Slycolate oxidise in Nicotiana tabacum varieties were studied at various growth stages. The enzyme activities of young leaves with rapid growth were different from those of old ones with stationary growth. In young leaves, activity of nitrate reductase was higher than that in mature ones and amylase activity was fairly constant in all stages. Activities of glycolate oxidise and peroxidase were found to be significantly lower in young leaves than in mature ones. Activity of glycolate oxidase in mature middle leaves was 2.45 times higher than that of young ones and inhibited by 36% when the enzyme was treated with 0.16 mM isonicotinic hydrazide.

• Journal of Plant Biology
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• v.26 no.4
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• pp.207-216
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• 1983

Mature embryo and developing seedlings of Ginkgo biloba L. were embedded in a paraplast and serially sectioned at 10${\mu}{\textrm}{m}$ to examine vascular differentiation and vascular transition. Procambium and protophloem formed a continuous system along the epicotylhypocotyl root axis and cotyledons in mature embryo, whereas protoxylem was differentiated discontinuously in the cotyledons and rarely in the upper hypocotyl. The traces of the first and second leaf primordia apeared almost at the same time oppositely to each otehr at the epicotyl and alternately with the cotyledon traces in the upper hypocotyl. The trace differentiated bidirectionally toward the epicotyl and root tips. the young root initially formed a diarch xylem. Then, as the traces of the first and second leaves were superimposed, the diarch xylem. Then, as the traces of the first and second leaves were superimposed, the diarch xylem of the root was changed totriarch and tetrarch xylem, respectively. On the formation of primary vascular system of Ginkgo biloba, it is suggested that the primary phloem forms a continuous system throughout the seedling, whereas the primary xylem of the epicotyl is formed independently from that of the root-hypocotyl cotyledon system.