• Journal of Life Science
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• v.7 no.4
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• pp.322-328
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• 1997

Two phosphorylated proteins having molecular weights of 57kD and 72kD were detected from the slubilized membrane protein fraction of mating type a cells of Rhodosporidium toruloides which belongs to heterobasidiomycetous yeast. The phosphorylation of the protein was inhibited by a sexual pheromone, Rhodotorucine A (Rh. A), which is secreted from mating type a cells. On the other hand, counterpart mating type A cells and M-39 strain which is a styerile mutant derived from a cells, had also the same two phosphorylated proteins, However, the phosphorylation of the protein from A cells, and M-39 strain were not inhibited by the Rh. A. It suggests that inhibition of the phosphorylation reaction by the Rh. A in mating type a cells is a mating-type-specific reaction that relate to transduction mechanism of sexual pheromone signaling.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.1395-1398
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• 1997

Flexible parts comparing with rigid parts can be deformed by contact force during assembly. for successful assembly, information about their deformation as well as possible misalignment between mating parts is essential. Howecer, because of the complex relationship between parts deformation and reaction forces, it is difficult to acquire all required information from the reaction forces alone. In this paper, we measure parts deformation and misalignments by using the visual sensing system presented for flexible parts assembly. Experimental results show that the system can be effectively used for detecting parts deformation and misalignments between mating parts.

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.423-430
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• 2006

A total of 367 isolates of Phytophthora infestans was collected from the leaf samples of late blight disease from five provinces in Korea over the three growing seasons of 2002-2004. Of the 367 isolates, 337 isolates were of the A1 mating type, and 30 isolates were of A2 mating type, showing that the majority was A1 mating type. Profiles of Gpi and Pep defined four allozyme genotypes among the total of 367 isolates. All four allozyme genotypes could be distinguished on the basis of Gpi profiles alone, whereas all isolates were homozygous at the Pep locus (100/100). The mitochondrial DNA haplotype of all isolates were the IIa haplotype. Amplification of the genomic DNAs extracted from eight isolates of each mating type by polymerase chain reaction with the selected primer (OPC-5 primer) produced a total of 20 DNA bands, of which 11 bands were polymorphic. According to the RAPD analysis using the OPC-5 primer, 106 isolates including two standard isolates were separated into 8 groups at the similarity level of 92.5%. The RAPD groups were not correlated with the allozyme genotypes and the isolated locations. All of the eight RAPD groups were identified in Gangwon-do, suggesting that Gangwon-do is the center of origin of the P. infestans in Korea. A 600-bp DNA band generated with the OPC-5 primer was specific to A1 mating type isolates, but not detected with A2 mating type, showing that the specific PCR primer can distinguish different mating types in P. infestans.

• 제어로봇시스템학회:학술대회논문집
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• 1996.10b
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• pp.632-635
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• 1996

Flexible parts can be deformed by the contact forces during assembly on the con to rigid parts and thus their successful assembly requires informations about their deformation as well as a misalignment between mating parts. However, because of the nonlinear and complex relationship between parts deformation and assembly reaction forces, it is difficult to acquire all required informations from only the reaction forces during assembly. In this paper, we propose a sensing system consisting of a camera and multiple mirrors for flexible parts assembly. Simulation results show that the system can be effectively used for detecting parts deformation and a misalignment between mating parts.

• Weed & Turfgrass Science
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• v.4 no.2
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• pp.135-143
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• 2015

Typhula ishikariensis Imai is a causal agent of Typhula snow mold, one of the most important turfgrass diseases in northern regions of the United States. Within Wisconsin isolates, there are three district groups clustered with known isolates of T. ishikariensis var. ishikariensis, var. canadensis and var. idahoensis as identified by RAPD markers. To further investigate the genetic relationship among these groups (varieties), monokaryon-monokaryon and dikaryon-monokaryon mating experiments were conducted. Mating types from var. ishikariensis, var. canadensis and var. idahoensis isolates were paired in all possible combinations. Pairings between var. canadensis and var. idahoensis were highly compatible, while no compatibility was detected between var. ishikariensis and either var. canadensis or var. idahoensis. These results indicate that var. ishikariensis is genetically separated from var. canadensis and var. idahoensis, whereas var. canadensis and var. idahoensis appeared to be genetically related to each other as a taxonomic unit. In the genetic relationship with the known biological species, var. ishikariensis and var. canadensis were genetically related to biological species I and II, respectively. However, var. idahoensis was not compatible with any of the biological species, suggesting that the pathogen may be in the process of biological speciation from var. canadensis.

• Tribology and Lubricants
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• v.35 no.2
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• pp.139-147
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• 2019

This study investigates the effects of corrosion resistance characteristics of opponent materials in relative motion on the sliding wear behavior of mild carbon steel. Pin specimens made of mild carbon steel are tested at several sliding speeds against mating discs made of two types of alloyed steels, such as type D2 tool steel (STD11) and type 420 stainless steel (STS420J2), with different corrosion resistance characteristics in a pin-on-disc type sliding wear test machine. The results clearly show that the sliding wear behavior of mild carbon steel is influenced by the corrosion resistance characteristics of the mating disc materials at low sliding speeds. However, the sliding wear behavior at high sliding speeds is irrelevant to the characteristics because of the rising temperature. During the steady state wear period, the sliding wear rate of mild carbon steel against the type 420 stainless steel at a sliding speed of 0.5 m/s increases considerably unlike against the type D2 tool steel. This may be because the better corrosion resistance characteristics achieve a worse tribochemical reactivity. However, during the running-in wear period at low sliding speeds, the wear behavior of mild carbon steel is influenced by the microstructure after heat treatment of the mating disc materials rather than by their corrosion resistance characteristics.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1246-1251
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• 1997

When the membrane protein fraction of mating type a cells of heterobasidiomycetous yeast R. toruloides was phosphorylated in vitro, two phosphorylated proteins of 72Kd and 57Kd were detected on SDS-polyacryamide gel. The phosphorylation reaction was inhibited by rhodotorucine A(Rh. A) which is a sexual pheromone secreted by mating type A cells. The inhibition of phosphorylation by Rh. A was dependent on $Ca^{2+}$, and independent on $Mg^{2+}$ or calmodulin. When adding trigger peptidase(TPase) inhibitor, antipain, no inhibition of phosphory was observed. Also, by adding the trysin-digested product of Rh. A, the phosphorylation was inhibited as the action of Rh. A. From these results, it is expected that the inhibition of membrane protein phosphorylation should be caused by the digested product of Rh. A with TPase.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.34 no.1
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• pp.43-50
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• 2021

A Leg Mating Unit (LMU) is a device utilized during the float-over installation of offshore structures that include hyperelastic pads and mating part. The hyperelastic pads absorb the loads, whereas the mating part works as guidance between topside and supporting structures during the mating sequence of float-over installation. In this study, the design optimization of an LMU for the float-over installation of floating-type offshore structures is conducted to enhance the performance and to satisfy the requirements defined by classification society regulations. The initial dimensions of the LMU are referred to the dimensions of those used in fixed-type float-over installation because only the location and the number of LMUs are known. The two-parameter Mooney-Rivlin model is adopted to describe the hyperelastic pads under given material parameters. Geometric variables, such as the thickness, height, and width of members, as well as configuration variables, such as the angle and number of members, are defined as design variables and are parameterized. A sampling-based design sensitivity analysis based on latin hypercube sampling method is performed to filter the important design variables. The design optimization problem is formulated to minimize the total mass of the LMU under maximum von Mises stress and reaction force constraints.

• Proceedings of the KSR Conference
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• 2001.10a
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• pp.163-170
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• 2001

Axle Gearbox of the High Speed Train has rotational degree of freedom centered on the Axle Shaft Center Line, and constrained by the Reaction Arm connected to the Bogie Frame via Resilient Ring made of Rubber. This System is laid on the Power Train and can influence on the Power transmitted from Motor to Axle. The stiffeness of the Resilient Ring have to be selected for the Natural Frequency of this System do not overlap with the Teeth-mating Frequency. To confirm the Design Parameters, Calculation and Experiment were executed and compared.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.5 s.176
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• pp.1305-1313
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• 2000

In a co-rotating scroll compressor, both scrolls rotate on their fixed axes contrary to the conventional orbiting type scroll machine. This implies fixed locations and directions of the gas pressure force and sealing force. Because the tilting moment is mainly caused by interplay between the resultant force of above forces and bearing reaction force, the variation during one cycle is relatively small. Under real operation, this moment is balanced by the restoring moment created by the reaction between the baseplate and thrust bearing or between the scroll tip and baseplate. If these reactions become too large, greater torque is required due to increased friction in addition to the wear of mating parts. Consequently, appropriate study and minimization of tilting moment is important in the design of scroll machines. In this study, taking into account of the small variation of tilting moment during one cycle, we minimize the moment and thrust bearing reaction force by a properly designed back pressure chamber. As a result, for both the driving and driven scrolls, the tilting moment and the reaction force of thrust bearing can be minimized. And the stability is improved for all cases.