• Korean journal of applied entomology
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• v.57 no.3
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• pp.209-220
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• 2018

Mating disruption (MD) has been widely used to effectively control Grapholita molesta in apple orchards. A simultaneous mating disruption (SMD) techniques have been developed to control both G. molesta and G. dimorpha. This study was performed to determine the practical parameters to apply the SMD technique to field conditions. To determine the application amount of SMD lures, a dispenser containing 10 mg pheromone was placed at different numbers of trees in an orchard. Application at every other tree (= one dispenser per two trees) was relatively safe to expect effective MD efficiency in both wax and polyethylene (PE) formulations. One time application at the end of March was enough to maintain a year round MD efficacy against both species. A fence treatment using food trap was applied to prevent any immigratory mated females from nearby untreated regions. To enhance the food trap by adding host-derived secondary compounds, terpinyl acetate (TA) was screened to be effective to attract females of Grapholita molesta among six compounds contained in apple fruit extracts. Among different TA concentrations, 0.05% TA treatment was the most effective to attract the adults. A mixture of TA and sugar was effective to attract and kill females and called FAKT (female attract-to-kill techniques). FAKT was treated at approximately 6 m interval at the edge of the apple orchards. The females trapped by the FAKT included mated females possessing vitellogenic oocytes. SMD supplemented with FAKT maintained the high MD efficacy and significantly suppressed leaf damage induced by the two insect pests compared to control or single SMD treatment.

• Korean journal of applied entomology
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• v.49 no.1
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• pp.31-36
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• 2010

Mating disruption (MD) using synthetic sex pheromone lures has been used to control the Oriental fruit moth, Grapholita molesta (Busck), in apple orchards. In Korea, where several small apple orchards are clustered but independently managed, its efficacy has been suspected mainly due to immigration of any mated females from nearby untreated cultivating areas. This study developed an edge treatment technique to decrease any local MD-free zones in a specific MD-treated farm and to trap any immigrating mated females by installing MD lures and food traps around the apple farm with 10 meter intervals. The addition of the edge treatment to the MD significantly prevented leaf and fruit damages induced by G. molesta compared to MD only. Moreover, this study tried to optimize the MD control technique by determining frequency of MD application. It suggests two MD applications with the edge treatment at the end of March and at the early of July to be effective throughout the entire apple growing seasons.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2000.05a
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• pp.110-110
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• 2000

• Korean journal of applied entomology
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• v.61 no.1
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• pp.51-62
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• 2022

Numerous insect pests threaten the Korean orchard industry through feeding on various tissues of fruit trees. Generally, the control of economically important orchard pests is based on the use of chemical insecticides. Owing to growing concerns regarding the environmental and human health effects of insecticides, environment-friendly pest control strategies are urgently needed. Pheromones of orchard pests could lead to an environmentally safe control system based on mating disruption or mass trapping. This review summarizes the functions and compounds of known pheromones from 51 orchard pests in Korea. The pheromones identified to date from 14 species in the families Miridae, Aphididae, Diaspididae, Pseudococcidae, Rutelidae, Cecidomyiidae, and Eurytomidae and 26 species in the order Lepidoptera are female-produced sex pheromones that attract only males. In contrast, all known examples for 11 species in the families Alydidae, Pentatomidae, Thripidae and Cerambycidae are male-produced aggregation pheromones that attract both sexes. Research on pheromones in new pests, kairomones in key orchard pests, mating disruption dispensers to generate prolonged release of the pheromones, and trap design and trap location for mass trapping will be required for the expanded use of pheromones and other semiochemicals in orchard pest management in the future.

• Korean journal of applied entomology
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• v.53 no.2
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• pp.103-110
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• 2014

The efficacy of mating disruptors MD-IKR (Isomate-KR) and MD-CR (Confuser-R) for controlling Grapholita molesta (Busck) and Carposina sasakii (Matsumura) was evaluated in non-chemical or organic apple orchards. For G. molesta, the efficacy of MD-IKR and MD-CR in Chilgok was 99.8 and 99.8% (2011) and 92.4 and 96.7% (2012), respectively, showing different mating reduction values. The efficacy of MD-IKR and MD-CR in Cheongsong was 99.1 and 92.8% (2011) and 99.6 and 99.6% (2012), respectively. For C. sasakii, the efficacy of MD-IKR and MD-CR was 100% at Chilgok and Cheongsong in 2011 and 2012. Evaluation of the shooting and fruit damage rates showed that the efficacy of the mating disruptors in controlling G. molesta and C. sasakii was better than that of the non-control. However, there was no significant control difference between using the mating disruptors and the conventional control. No difference in the efficacy of the two mating disruptors was observed. We believe that MD-IKR could be an alternative to MD-CR.

• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• Korean journal of applied entomology
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• v.49 no.2
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• pp.139-144
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• 2010

This study investigated the efficiency of mating disrupters for the control of Grapholita molesta(Busck) and Adoxophyes orana in pear orchards. G. molesta was shown 4 peak occurrence, which were different according to treatments. In conventional control, many moths were captured in mid and late April and peaks occurred in mid June, August, and early-mid September. In the non-control treatment, there were low populations in April. A. orana also appeared to 4 peak in 2007. Moths were rarely captured in mating disrupter treatment but many were captured in both the non-control and in conventional treatment. A. orana damage differed significantly between mating disrupter use and conventional control in 2006. Especially, the difference was significant between conventional control and mating disrupter to leaf damage in August, and there were similar results in the damage by G. molesta in the shoots and fruits.

• Korean journal of applied entomology
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• v.47 no.3
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• pp.255-263
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• 2008

A wax-typed pheromone dispenser has been developed and applied to control outbreak of the oriental fruit moth, Grapholita molesta, in apple orchard. To optimize its application technique, this study analyzed effect of different amounts of the pheromone dispenser on mating disruption('MD') of G. molesta. Different pheromone dispenser amounts significantly influenced the MD effect assessed by cumulative male adult catches monitored respectively by sticky delta trap and food trap, and resulted in differential damage on host plants. In a field test during entire growing season, a standard amount(120 g per 0.117 ha) of wax-typed pheromone dispenser was proved to be effective to suppress outbreak of G. molesta adults and to prevent host plant damage as much as a current commercial MD product($Isomate^{(R)}$). This study also demonstrated an effectiveness of deployment of food trap barriers around MD-treated area to prevent immigration of mated females from outside untreated areas. These results indicate that the wax-typed pheromone dispenser can be applied to control field G. molesta populations and its co-application with fuod trap barriers would be optimal to maximize MD efficacy.

• Korean journal of applied entomology
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• v.54 no.4
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• pp.369-374
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• 2015

Grapholita molesta (GM) has been considered as a major problem to apple and peach orchards. Mating disrupters have been applied to control the pest over the world as an Integrated Pest Management [IPM] tool. Various types of dispensers releasing mating disrupters have been developed with different formulation of wax, polyethylene tube and aerosol. In this study, the dispensers that were composed of ester waxes and polypropylene (PP) film were used as dispenser materials to analyze release pattern of GM pheromone compared with paraffin wax and polyethylene (PE) film. The release pattern in PP film group was consistent with time while PE film group irregular. Based on these results, Japan wax_PP film dispenser (Japan_PP) was manufactured and showed the mating disruption effect of 98% during 5months in peach orchard.

• Korean journal of applied entomology
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• v.59 no.4
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• pp.333-339
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• 2020

Pheromone-based techniques are becoming a viable strategy of insect pest management as facilitated by the exponential increase in numbers of pheromone identifications from many insect pests. This is the report on the efficacy of pheromone-mediated chemical communication disruption (PCD) technique against the Korean population of smaller clearwing moths, Synanthedon tenuis (Butler) (Lepidoptera: Sesiidae) using its female sex pheromone component, (Z, Z)-3, 13-octadecadien-1-ol. The PCD trials were carried out four times during 2016 and 2017 in persimmon orchards located at Suncheon and Jinju Cities in Korea, and the PCD efficacy was expressed as the mean differences in the seasonal catches of S. tenuis males in the PCD and control plots. The seasonal male moth catches in monitoring traps installed in the PCD plots were significantly lower as compared with those installed in the control plots. Consequently, the PCD efficacy in the experimental orchards ranged from 95.2-100% with an average efficacy of 98.8 ± 1.2%, revealing a future possibility of pheromone-based management of S. tenuis.