• Journal of Preventive Medicine and Public Health
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• 제38권4호
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• pp.437-442
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• 2005

Objectives : DNA methylation is one of the best characterized epigenetic mechanisms that play a regulatory role in genome programming and imprinting during embryogenesis. In this present study, we investigated the association between DNA methylation in the human placenta and the maternal folate and homocysteine concentrations on the Methylenetetrahydrofolatereductase (MTHFR) genetic polymorphism during pregnancy. Methods : We investigated 107 pregnant women who visited Ewha Woman's University Hospital for prenatal care during their $24{\sim}28$ weeks-period of gestation. During the second trimester, we measured the serum homocysteine and folate concentrations . The MTHFR 677 genetic polymorphism was determine by performing PCR-RFLP assay. The expression of DNA methylation in the human placentas was estimated by using immunohistochemistry method. Results : Serum folate was negatively correlated with the serum homocysteine concentration for all the MTHFR genotypes. We found positive correlation between the folate concentrations and the DNA methylation in the human placenta (p<0.05). An increasing concentration of homocysteine was associated with reduced DNA methylation in the human placenta. The coefficient value was -2.03 (-3.77, -0.29) on the regression model (p<0.05). Conclusion : These findings suggest that the maternal folate and homocysteine levels along with the MTHFR 677 genetic polymorphism during pregnancy affect the DNA methylation in the human placenta.

• Journal of Animal Science and Technology
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• 제59권1호
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• pp.1.1-1.11
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• 2017

Background: Intrauterine environment plays a pivotal role in the origin of fatal diseases such as the metabolic syndrome. Diabetes is associated with low-grade inflammatory state and dysregulated adipokines production. The aim of this study is to investigate the effect of maternal diabetes on adipocytokines (adiponectin, leptin and TNF-${\alpha}$) production in F1 offspring in rats. Methods: The offspring groups were as follows: F1 offspring of control mothers under control diet (CD) (CF1-CD), F1 offspring of control mothers under high caloric diet (HCD) (CF1-HCD), F1 offspring of diabetic mothers under CD (DF1-CD), and F1 offspring of diabetic mothers under HCD (DF1-HCD). Every 5 weeks post-natal, 10 pups of each subgroup were culled to obtain blood samples for biochemical analysis. Results: The results indicate that DF1-CD and DF1-HCD groups exhibited hyperinsulinemia, dyslipidemia, insulin resistance and impaired glucose homeostasis compared to CF1-CD (p > 0.05). DF1-CD and DF1-HCD groups had high hepatic and muscular depositions of TGs. The significant elevated NEFA level only appeared in offspring of diabetic mothers that was fed HCD. DF1-CD and DF1-HCD groups demonstrated low serum levels of adiponectin, high levels of leptin, and elevated levels of TNF-${\alpha}$ compared to CF1-CD (p > 0.05). These results reveal the disturbed metabolic lipid profile of offspring of diabetic mothers and could guide further characterization of the mechanisms involved. Conclusion: Dysregulated adipocytokines production could be a possible mechanism for the transgenerational transmittance of diabetes, especially following a postnatal diabetogenic environment. Moreover, the exacerbating effects of postnatal HCD on NEFA in rats might be prone to adipcytokine dysregulation. Furthermore, dysregulation of serum adipokines is a prevalent consequence of maternal diabetes and could guide further investigations to predict the development of metabolic disturbances.

• Fisheries and Aquatic Sciences
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• 제9권4호
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• pp.140-145
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• 2006

We measured activities of the ubiquitous tripeptide non-protein thiol (L-${\gamma}$-glutamyl-L-cysteinyl-glycine), glutathione (GSH), which is believed to playa fundamental role in detoxifying xenobiotics in biological systems, as a metabolic biomarker for benzo(${\alpha}$)pyrene and Aroclor 1254 exposure in the Pacific oyster Crassostrea gigas. Reproductive oysters were exposed to the pollutants for 50 days by the algal vectoring method in which the oysters were fed with concentrated standard algal foods grown in culture media containing Aroclor 1254 (0, 5, 50, 500 ng/g) or benzo(${\alpha}$)pyrene (0, 10, 100, 1,000 ng/g). Both pollutants induced maternal GSH activities in 10 days in a dosage-dependent manner (p<0.05), although Aroclor 1254 was stronger. The pollutant-driven GSH elevation persisted for 20 to 30 days depending on the pollutants and concentrations. Thereafter, a drastic decline in the GSH activity was observed due to metabolic failure, after which the oyster GSH remained at low levels throughout the remainder of the experiment. The pollutant exposures influenced maternal reproductive output in terms of fertilization, hatching, and morphology. These results imply that changes in activity of the GST-catalyzing molecule can be used as an oyster biomarker for Aroclor 1254 and benzo(${\alpha}$)pyrene exposure.

• 가정과삶의질연구
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• 제32권2호
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• pp.77-97
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• 2014

The purpose of this study was to examine 1) the relations among parent-reported, teacher-reported, and task-observed effortful control, and 2) the differences of preschoolers' effortful control based on preschoolers' gender and parenting behaviors (affectionate, controlled). In this study, the subjects were 221 preschoolers (104 boys, 117 girls) and their parents. The preschoolers' effortful controls was measured by Effortful Control Battery (Snack Delay, Turtle and Rabbit, and Red-Green Sign) and the Children's Behavior Questionnaire-Short Form. Parenting behavior was measured by the Parenting Style scale. The major results of this study were as follows. First, there were positive correlations between parent-reported effortful control and both teacher-reported and task-observed effortful control. Second, there were significant differences in preschoolers' effortful control by gender and level of affectionate parenting behavior. And there was an interaction effect between gender and level of affectionate parenting behavior on preschoolers' effortful control. The implications of this study were as follows. It is needed to evaluate effortful control in various circumstances and by multiple raters, and affectionate parenting behavior had an important role upon boys' effortful control rather than that of girls.

• Clinical and Experimental Reproductive Medicine
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• 제47권2호
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• pp.122-129
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• 2020

Objective: The survival of a semi-allogeneic fetus depends on several immunological mechanisms, and it has been suggested that recurrent pregnancy loss (RPL) could develop as a result of one or more immunological abnormalities. Methods: Compatibility between partners for human leukocyte antigen (HLA) genotypes and the relationships between maternal killer-cell immunoglobulin-like receptor (KIR) and paternal HLA-Bw4/Bw6 and HLA-C1/C2 supra-groups were investigated in 25 couples with RPL in comparison to healthy couples with children. HLA and KIR genotyping was performed using polymerase chain reaction with sequence-specific primers and/or sequence-specific oligonucleotides. Results: HLA class I incompatibility between partners, especially in HLA-B alleles, was more common in the RPL group (p= 0.01). HLA-C2 homozygosity was more frequent in the male partners of RPL couples than in other groups (p= 0.03). The KIR2DL5 gene frequency was significantly higher in both the female and male partners of RPL couples, whereas the KIR2DS3 gene frequency in male partners of RPL couples was significantly reduced (p= 0.03). The presence of KIR2DL3 in women with RPL was correlated with the presence of HLA-C2 alleles in their spouses (p= 0.03). Conclusion: Our data from a Turkish population suggest that male HLA-C2 homozygosity may play an important role in RPL. Additionally, an incidental match between male HLA-C2 and female HLA-C1 ligand KIR receptors might perturb the balance between activatory and inhibitory KIR-ligand interactions during pregnancy in couples affected by RPL. The roles of orphan KIR2DL5 and orphan KIR2DS3 in RPL remain obscure.

• 한국수정란이식학회지
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• 제29권4호
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• pp.375-383
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• 2014

Klotho (KL) is a single transmembrane protein composed of KL1 and KL2 repeats possessing ${\beta}$-glucuronidase activity and maintains calcium homeostasis in physiological state. It has been implicated in pigs that calcium is important for the establishment and maintenance of pregnancy, and our previous study has shown that transient receptor potential vanilloid type 6 (TRPV6), a calcium ion transporter, is predominantly expressed in the uterine endometrium during pregnancy in pigs. However, expression and function of KL in the uterine endometrium has not been determined in pigs. Thus, the present study determined expression and regulation of KL in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that levels of KL mRNA decreased between Days 12 to 15 of the estrous cycle, and its expression showed a biphasic manner during pregnancy. KL mRNA was expressed in conceptuses and in chorioallantoic tissues during pregnancy. Explant culture study showed that expression levels of KL were not affected by treatment of steroid hormones or interleukin-1beta during the implantation period. Furthermore, levels of KL mRNA in the uterine endometrium from gilts carrying somatic cell nuclear transfer (SCNT)-derived embryos were significantly lower than those from gilts carrying natural mating-derived embryos on Day 12 of pregnancy. These results exhibited that KL was expressed at the maternal-conceptus interface in a pregnancy status- and stage-specific manner, and its expression was affected by SCNT procedure, suggesting that KL may play an important role in the establishment and maintenance of pregnancy in pigs.

• 여성건강간호학회지
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• 제28권1호
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• pp.8-17
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• 2022

Purpose: Although the term "transition to motherhood" is commonly used in research, the concept is not clear. This study, hence, was conducted to clarify the concept of "transition to motherhood." Methods: The concept analysis framework developed by Walker and Avant is used to analyze the concept of transition to motherhood. Results: Transition to motherhood is defined as the physical, psychological, social, and relational (mother-baby relationship/interpersonal relationship) changes that happen to a woman after pregnancy and delivery of a baby. The attributes of the transition to motherhood include: 1) adapting to physical changes after pregnancy and childbirth; 2) experiencing various psychological changes; 3) changing of her social perception from being a woman to someone's mother; and 4) forming and developing a relationship with the newborn, adjusting priorities, and redefining the relationship between family and others. Meeting the newborn is regarded as an antecedent of the transition to motherhood. Redefining identity and physical image, ensuring mother's well-being, maternal attachment, and confidence in the maternal role are regarded as consequences of the transition to motherhood. The concept was clarified by the presentation of model, borderline, and contrary cases. Conclusion: The significance of this study lies in the clarification of the concept of transition to motherhood and defining its attributes. It is recommended that tools be developed to measure transition to motherhood based on the results of this study. Furthermore, nurses and midwives can use study findings to better understand the concept of transition to motherhood in providing care and support to mothers who experience it.

• Perspectives in Nursing Science
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• 제11권2호
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• pp.101-108
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• 2014

Purpose: The purpose of this study was to compare parenting stress between married migrant women and Korean women. Methods: Subjects were 68 married migrant women and 70 Korean women raising children aged seven years or younger, living in three different cities in Gangwon Do. To measure parenting stress, we used Kim's (1997) questionnaire that had been modified from Richard & Abidin's (1990) Parenting Stress Index (PSI). Results: There were significant differences between the two groups in age, academic credentials, job, monthly income, and achievement of maternal role. No statistically significant differences were found for parenting stress scores (p<.355), but there was a significant difference in the child characteristics of parenting stress (p<.007) between the two groups. There were no differences in the demographic variables influencing parenting stress between the two groups. Conclusion: The development of multi-cultural parenting and family support programs should consider the nationality and marital satisfaction of the family.

• 가정과삶의질연구
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• 제19권3호
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• pp.41-51
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• 2001

The purpose of this study was to investigate the relationships between mothers involvement of their childrens peer interaction, their memory of childhood peer relations, and their perception of childrens social competence. Subject consisted of 302 mothers of 4- to 6-year-old children. A sample of mothers completed a series of questionnaires assessing their peer experiences, perceptions about their childrens sociability, and a behavioral checklist of their involvement activities. The results indicated that mothers own sociability and their perceptions of their childrens sociability influenced their involvement. More intense maternal involvement occurred when mothers perceived their children to be sociable. Mothers with sociable peer recollections appeared to take more active role in their childrens social development.

• Asian-Australasian Journal of Animal Sciences
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• 제30권4호
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• pp.449-454
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• 2017

Recently, increasing attention has been paid to the welfare of farm animals, which have been evaluated using behavioral and physiological measures. However, so far, the measures have almost always been used to estimate poor welfare. In this review, firstly we focus on how oxytocin (OT) relates to positive social behavior, pleasure, and stress tolerance, and second on which management factors stimulate OT release. OT induces maternal and affiliative behaviors and has an anti-stress effect. Further, OT is produced during enjoyable events, and has positive feedback on its own release as well. Therefore, to some extent, the relationship of OT to positive normal behavior was mutually beneficial-heightened OT concentration owing to comfortable rearing conditions induces positive social behavior, which in turn may increase OT concentration. Hence, studies on animal welfare should pay more attention to increasing comfort and the stress tolerance, rather than only focusing on when stress occurs in farm animals.