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Seeding Soils and Tray Types Mediate Growth Characteristics of Perilla Seedlings (상토 및 트레이 종류에 따른 종실용 들깨의 육묘 특성)

  • Park, Jin-Ki;Han, Won-Young;Han, Kil-Su;Ryu, Jong-Soo;Won, Ok-Jae;Jeong, Tae-Uk;Yoon, Young-Ho;Bae, Jin-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.1
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    • pp.63-71
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    • 2020
  • The purpose of this study was to analyze the growth characteristics of perilla according to the materials of the seedbed for the development of seedling plug technology suitable for the mechanical transplantation of perilla. Perilla (Perilla frutescens var. japonica Hara) cultivars Deulsaem and Sodam were used in this experiment. The composition ratios of 170 products from 16 companies published in the 'Korean Association of Seedbed Media' homepage were compared according to usage and type, and 11 products that corresponded to the average were selected. The seedbed was classified according to the seedbed for paddy rice as light-weight, semi-weight, and weight, and based on the seedbed for horticulture, as light-weight and ultra-light. The seedlings were placed in 72-cell (semi-automatic), 128-cell (automatic) and 220-cell (automatic) plug trays. We selected 2 light-weight seedbeds of paddy rice and 2 light-weight seedbeds of horticultural products with the highest plant growth. We analyzed plant height and mat formation of the perilla roots. Results showed that the perilla height and mat formation were the best in light-weight seedbeds of paddy rice (product R1). Therefore, light-weight seedbeds of rice (product R1) were suitable for perilla plant transplantation. The estimated major components were vermiculite 41.0%, cocopeat 31.0%, peat moss 5.7%, and red-yellow soil 20.0%. The mechanical transplantation of perilla significantly boosts plant growth and reduces sowing and thinning efforts. However, continuous evaluation of newly introduced, commercial seedbeds is needed.

Ecological Evolution by Competitive Exclusion / An Experimental Approach with Cellular Slime Mold , Polysphondylium pallidum (경쟁배타에 의한 생태적 진화: 세포성 점균 Polysphondylium pallidum에 대한 실험적 접근)

  • ;Robert M. Eisenberg
    • The Korean Journal of Ecology
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    • v.17 no.3
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    • pp.299-310
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    • 1994
  • Intraspecific clonal interactions have important influences on a population structure of the cellular slime mold (CSM). This study was to investigate whether or not evolutionary change in a population could be induced by clonal competition, and to elucidate how various clones in a population evolve in a homogeneous environment of laboratory culture. The characteristic clones of Polysphondylium pallidum which had different resource consumption rates (RCR) and mating types I and II were selected for study. Investigation was conducted for 4 experimental time interval $(T_0-T_4)$; one experimental time interval took almost 10-14 days from inoculation to havest of fruiting bodies. Two sets of 50 clones were cultured from 50 clones at To, and RCR variations of the population were compared between $(T_0\;and\;T_4)$ for each set of clones. Each clone of the CSM had a diverse resource consumption rate, or growth rate, in a homogeneous and limited Cerophyl agar plate despite the passage of 48-56 generations from the beginning of the experiment. Diverse clones with different growth rate could coexist in one site of the homogeneous agar plate as well as heterogeneous soil microenvironment. When there was high clonal diversity of RCR, a clone in a population had high chances to encounter other clones with resultant increased clonal competition. In one set, 26 of 37 clones of mating type I were changed to mating type Il for the 4 experimental time intervals, which indicated that the rate of competitive exclusion among clones during total experiment from $(T_0\;to\;T_4)$ was 0.703. In another set, 31 of 37 clones of mating type I were changed to mating type II , having the rate of competitive exclusion 0.838. The frequency of each of mat~ng types changed by 0.93-1.29% in each successive generation. The competitive exclusion among clones occurred by 1.26-1.75% when approximately $2.6{\times}10^8$ bacterial cells were provided as food and thereafter one generation of myxamoebae of CSM elapsed at room temperature. This finding implicated that in the vegetative state of P, pallidurn there was 1.26-1.75% probabil~ty of evolutionary change per generation changing from one clone to another clone.

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A Study on Pull-out Capacity and Shear resistance strength change by grasses (초본류에 의한 인발저항력 및 전단저항력 변화에 관한 연구)

  • Kim, Taegyun;Chae, Soo Kwon;Chun, Seung Hoon
    • Journal of Wetlands Research
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    • v.15 no.4
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    • pp.431-440
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    • 2013
  • It was tested in field that a Pull-out Capacity and Shear resistance strength change of reed, common reed and sedge which were planted by mat-type turf and used for revegetation of bank. The testes were done for 9 weeks from end of May and the grasses were planted on sandy soil. Roots grew fastly after planted and increasement of a common reed and sedge root were reduced after 4 weeks but increasement of reed roots were not reduced. The difference of increasement of roots is due to a difference of propagation method. Sedge propagate by seed. Reed and commom reed propagate by seed and subterranean stem and reed has bigger subterranean stem than common reed. So increasement of common reed and sedge roots were slow than reed. By root growth pattern, increasement of pull-out capacity and shear resistance strength showed very similar way of root growth, those of common reed and sedge were fast in early stage of cultivation but were reduced. But increasement of pull-out capacity and shear resistance strength of reed was not reduced. A Maximum shear resistance strength called critical shear resistance strength of common reed and sedge can be Analyzed at 11 weeks after planted.

Giant Colonial Morphology of 10 Clusters of Rapidly Growing Mycobacteria Including Mycobacterium fortuitum-chelonei Complex on 7H10-Crocin Agar Medium (Mycobacterium fortuitum-chelonei complex 및 신속발육 Mycobacteria 10개 균집합체의 거대집락형태)

  • Choi, Chul-Soon;Jeon, Ho-Jin;Yang, Yong-Tae
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.3
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    • pp.363-373
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    • 1986
  • We developed a giant colony test system with rapidly growing mycobacteria by stab-culture with a loopful inoculum of cells into Middlebrook 7H10 agar medium containing soluble extracts of the fruits of Gardenia jasminoides(7H10-crocin agar medium) and assessed the significance of the giant colony test with 28 strains of 10 clusters of rapidly growing mycobacteria classified by the simple biological 5-test characters. Of the 10 clusters of mycobacteria tested, some of strains which belonged to cluster No. 1a, 5a and 11a did grow as gravis types, whereas most of other clusters gave mitis or intermedius types in their colonial sizes at 12 days culture. By this test, pathogenic strains of M. fortuitum-chelonei complex which belonged to cluster No. 5a, b, 7a and 8a, b could be divided into gravis, intermedius and mitis colony types and the gravis ones were characterized by bluish-white "mushroom-shaped" colonies with central complexes in the texture, whereas the intermedius gave grayish-white "flower-shaped" colonies with radiated folds, but without any central complexes. The mitis colonies were characterized by grayish-white smooth or smooth mucoid colonies and were common among the clusters in their shapes. The colony of M. chelonei was bluish-white mitis type and was characterized by its hilly rhizoid colony. The gravis colony of cluster No. 1a identified as M. phlei was characterized by yellow "round straw- mat-shaped" or "chrysanthemum-shaped" colony with whole complexes in the texture, and the gravis colonies of the cluster No. 11a gave grayish-white "flower-shaped" colonies with central stamens, radiating trough and fine cup-shaped strands in the texture. The four colony types of pathogenic species of M. fortuitum-chelonei complex on 7H10-crocin agar medium were distinctive from those of other clusters of rapidly growing mycobacteria and these results indicated that the giant colony test, in conjunction with the simple 5-test characters, would be of value in the differentiation of M. fortuitum complex from other clusters of rapidly growing mycobacteria.

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Construction of a Pure Cryparin-null Mutant for the Promoter Analysis of Cryparin Gene (Cryparin 유전자의 promoter 분석을 위한 cryparin 유전자 치환체의 순수 제조)

  • Kim, Myoung-Ju;Yang, Moon-Sik;Kim, Dae-Hyuk
    • The Korean Journal of Mycology
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    • v.26 no.4 s.87
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    • pp.450-457
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    • 1998
  • The cryparin of Cryphonectria parasitica belongs to a cell wall associated fungal hydrophobin. The cryparin, though it is encoded by a single copy gene, is known for the high expression during the liquid culture of C. parasitica, and it turns out that 22% of total mRNA was transcribed for cryparin at 48hr after the liquid culture. In addition, it is also known as one of down-regulated fungal proteins by the presence of double stranded RNA virus, Cryphonectria hypovirus 1. In previous studies (Kim et al., 1999), we have constructed a cryparin-null mutant by replacing the cryparin gene with hygromycin B resistance gene due to site directed homologous recombination. In order for the promoter analysis of cryparin which seems to be very strong as well as mycoviral specific, it is preferable to have a strain with only a target promoter replaced and a discernable target site for incoming vectors. However, the cryparin-null mutant revealed the presence of an additional copy of transforming vector except the one which replaced the cryparin gene. In addition, the cryparin-null mutant did not contain any markers for targeted integration of incoming vectors. This prompts us to design an experiment to obtain a strain for promoter analysis of cryparin gene. A different mating type strain EP6(Mata, $met^-$) was mated with the cryparin-null mutant ${\triangle}$Crp194-7(MatA, Crp${\triangle}$::hph) to make the progenies with only a single replacement vector and $met^-$ characteristic remained. Nutritional assay as well as Southern blot analysis revealed that the progeny, ${\triangle}$Crp194-a6, was the methionine auxotroph with a single replacing vector in genome. Northern blot analysis and PAGE showed that there was no cryparin produced in this bred strain either.

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Geospatial Data Modeling for 3D Digital Mapping (3차원 수치지도 생성을 위한 지형공간 데이터 모델링)

  • Lee, Dong-Cheon;Bae, Kyoung-Ho;Ryu, Keun-Hong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.27 no.3
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    • pp.393-400
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    • 2009
  • Recently demand for the 3D modeling technology to reconstruct real world is getting increasing. However, existing geospatial data are mainly based on the 2D space. In addition, most of the geospatial data provide geometric information only. In consequence, there are limits in various applications to utilize information from those data and to reconstruct the real world in 3D space. Therefore, it is required to develop efficient 3D mapping methodology and data for- mat to establish geospatial database. Especially digital elevation model(DEM) is one of the essential geospatial data, however, DEM provides only spatially distributed 3D coordinates of the natural and artificial surfaces. Moreover, most of DEMs are generated without considering terrain properties such as surface roughness, terrain type, spatial resolution, feature and so on. This paper suggests adaptive and flexible geospatial data format that has possibility to include various information such as terrain characteristics, multiple resolutions, interpolation methods, break line information, model keypoints, and other physical property. The study area was categorized into mountainous area, gently rolling area, and flat area by taking the terrain characteristics into account with respect to terrain roughness. Different resolutions and interpolation methods were applied to each area. Finally, a 3D digital map derived from aerial photographs was integrated with the geospatial data and visualized.

A Study on the Selection of Vegetation and Method for the Revegetation on the Highway Slope in Consideration of the Surrounding Environment (고속도로(高速道路) 비탈면(面)에서 주변환경(周邊環境)을 고려(考慮)한 녹화공법(綠花工法)과 식생선발(植生選拔)에 관(關)한 연구(硏究))

  • Woo, Bo-Myeong;Jeon, Gi-Seong;Kim, Kyung-Hoon;Choi, Hyung-Tae
    • Journal of Korean Society of Forest Science
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    • v.85 no.4
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    • pp.619-633
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    • 1996
  • This study was carried out from March 1995 to February 1996 to select suitable vegetation and measures for revegetation on the highway cut-slope in consideration of the surrounding environment in the middle region of Korea. The numbers of revegetation-measures type surveyed were eighteen measures as a whole in descending order of the Seed-spraying measures, Block-sod pitching measures, and Straw-mat mulching measures. The numbers of vegetation species by highway line were 243 in Chungbu, 223 in Jungang, 210 in Kyongbu 2, 199 in Kyongbu 1, 179 in Sohaean, 163 in Seoul circulation. 148 in Shingal-ansan, 141 in Second kyongin and 123 in Kyongin respectively. The highest index of species diversity(H') was in Kyongbu 1-line and the evenness(E') on upper site was greater than that of the lower site. The revegetation measures were selected in consideration of nature of soil, gradient, area and situation, and 83 species were selected as those for revegetation measures in highway cut-slope.

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Dressing Effect of Phosphorus Fetilizer on the Growth of Soil Improving Species (비료목생장(肥料木生長)에 미치는 인산비료(燐酸肥料)의 시비효과(施肥效果))

  • Ma, Sang Kyu
    • Journal of Korean Society of Forest Science
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    • v.45 no.1
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    • pp.26-36
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    • 1979
  • Through several trials that has done for making the fertilizing-counter plan on the soil improving species, some results have been got as follows; 1. In the non-phosphorus dressing plots soil improving species have very poor survial ratio and show us to be died step by step. It may be resons that root can not make the nodule in case of non-phosphorus dressing and so tree could not absorb the nitrogen nutrient fixed by the nodule. And root competition with the weedy speces for utilizing the nutrient and oxygen in the soil could be reasons when planting in the heavy weedy rooting site. 2. Triple super phosphate, Fused Mg Phosphate and Fused super phosphate have showed the remarkable effects on the growth of soil improving species within 3rd year after planting. But Koreaan tablet fertilizer(9-12-4) for forest purpose have reacted considerably lower effect in comparision with the above powder and grain type phosphorous fertilizer. 3. In case of tablet type fertilizer tree root will have very little phosphorus absorbing surface because phosphorus can be utilized only from the tablet surface and root can not penetrate into the tablet. This my be reson to show the poor dressing reaction of tablet fertilizer but tablet fertilizer has a possibility to be utilized during long years as a sympton in photo 6. So tablet fertilizer can have a recommendation to dress much fertilizer at p]anting year and then tree root can get much more chance for absorbing the phosphorus that could keep the high survival and for utilizing it during many years without after dressing. 4. The granurar and powder type phosphate can develop the dense root mat like photo 8 because of giving the large surface for absorbing the phosphorus and weedy root can approch to the nodule for taking the nitrogen element. So this type seems to present better effect than tablet type to control the soil movement, stem weight as 200g per meter(l meter long${\times}$0.1m width). When added the lime any effect could not be found and rather give the negative effect. So Lespedeza seed sowing and phosphorus dressing system seems us to be very reasonable method for covering the raw material of basket making, fodder and fuel wood supply. 7. Fused Mg phosphate and Fused super phosphate are good fertilizer to the soil improving species and dressing more than 30g per seedling can be recommendable amount. 5. In the unproductive and dry soil with phosphorus fertilizer Robinia pseudoacacia and Alnus firnui can grow more than 2.3m in height at 3rd year and Alnus inokumae have the rapid height growth that is more than 1.8m at 2nd year. Depending on the growth situation like the above example minirotated management has possibilities and rapid covering of erosed land can be done with the soil improving species and phosphorus fertilizer. 6. In the Lespedeza sowing plot with 40g Fused Mg phosphate dressing per meter in the eroded and unproductive forest soil Lespedeza have completely covered this poor land and produced the green.

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A Study on the Basic Planning of the Nam-Hae Sin-Sa Architecture (남해신사 기본계획에 따른 신당건축 고찰)

  • Kim, Sang Tae;Jang, Hun Duc
    • Korean Journal of Heritage: History & Science
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    • v.42 no.2
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    • pp.62-85
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    • 2009
  • The Nam-Hae Sin-sa, the South Sea shrine in Yeong-Am, Korea was a national institution for public peace and bliss, was excavated in 2000, and the shrine and the 3-way-gate were reconstructed in 2001. Hae Sin-sa, the Sea shrine is a place for religious service separated into the Nam-Hae Sin-sa, the Dong-Hae Myo, and the Seo-Hae Dan. The Dong-Hae Myo was reconstructed, but restored shrine and 3-way-gate of the Nam-Hae Sin-sa is not perfect in comparison with excavation plan in 2000, therefore new reconstruction was researched through the related literature, the analysis of historical maps and excavation results, the interview with the concerned people and the case study. This research defines the analysis of the Plan of the Nam-Hae Sin-sa Reconstruction as follows. 1. The Nam-Hae Sin-sa was the institution for religious service operated by national direct management, represents the shrine for public peace and bliss on the Mountain, the Sea, and the River. Especially the Nam-Hae Sin-sa had an important position on the pivot of international trade with China and Japan, and had a role of main shrine with another one in the Mt. Ji-ri San. 2. The name of the Sea shrine was called as Nam-Hae Sin-sa(the South Sea shrine), Dong-Hae Myo(the East Sea shrine), Seo-Hae Dan(the West Sea shrine). But the name of the South Sea shrine had changed in the early period of Chosun as Nam-Hae Sin-sa to the later Chosun as Nam-Hae Dang through the research of related literature and historical map. Such as the Seo-Hae Dan, it was constructed for the Dan, the flat raised-floor without buildings, and changed to the type of Sa-Dang with addition of buildings. 3. The historical map of Hae Sin-sa informs the types of the roof, the Mat-bae roof was used in the Dong-Hae Myo, but the Pal-jak roof was showed in the Seo-Hae Dan and the Nam-Hae Sin-sa. 4. According to the analysis of Yong-Ch'uck the unit length, Nam-Hae Sin-sa was reconstructed in the period of Koryo on large scale, but it was restored in the Chosun on middle scale. And the Unit of Yong Ch'uck was changed into Yeong-jo Ch'uck in the period of Chosun. 5. As the results, The Plan of the Nam-Hae Sin-sa Reconstruction designed the new shrine into the 3 Kan front and the 2 Kan side with 3:2 scale. An-ch'o-gong with Yong-du and Yong Mi the ornaments represents head and tail of dragon, the Un-gong and the ornament of Pa-ryun-dae-gong in the building, and the Ch'ung-ryang of the Yong-du show the image of the institution for religious service for the god of the sea who look like dragon. The inner gate building and the main entrance were designed as same plan and scale as Hyang-gyo, the Korean Traditional School and Shrine of Confucianism, on the basis of results of excavation. Raise the 3-tall gate of the main entrance with harmony of the scale and the shape, because the side of gate building has the Mat-bae roof. 6. This research shows that Plan of the Nam-Hae Sin-sa Reconstruction is composed into shrine space and reservation space from the main entrance to inner gate and shrine like Jung-ak Dan in the Mt. Gye-ryong San, and it also informs the well in the west side of Sin-sa is an important factor of the plan of shrine architecture.

SPECIES OF CULTIVATED PORPHYRA IN KOREA (한국산 양식김의 종류)

  • KANG Jae Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.2
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    • pp.77-92
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    • 1970
  • Ueda, in the course of his systematic work on the lavers, Porphyra, in Japan and Korea in 1932, mentioned that most of the cultivated Porphyra belong to Porphyra tenera Kjellman. Then he, dividing the species into two forms, f. typica and f. kjellmani, put Korean cultivated Porphyra under the latter. From the 1930s to the early 1940s, Fujikawa, Kaneko and others worked on Physiological experiments or cultivational experiments of Porphyra in the culture-bed, but there was no mention about the cultivated Porphyra species. However, many fishermen generally recognize that the characteristics of cultivated Porphyra vary depending on their habitat or the picking season, and it is considered that these differences are due to the varieties of the species which are well adaptable to various environments. Recently, I have become aware of the predominant occurrence of P. yezoensis Ueda in most culture-beds of Korea as in the Tokyo Bay or other places in Japan. At present, since artificial seeding for the cultivation of Porphyra with Conchocelis has been carried out and peculiar species can be cultured, a study of the species of cultivated Porphyra has become an important subject. I collected the specimens from a number of culture-beds which are located in the legions shown in fig. 1 from January, 1968 to May, 1970 and found that there are five species, P. tenera Kjellman, P. yezoensis Ueda, P. kuniedai Kurogi, P. seriata Kjellman and P. suborbiculata Kjellman. Among them, P. kuniedai was treated as a round-type, a form of P. tenera, by Kunieda (1939) and Tanaka (1952) and the occurrence of this form is generally recognized by most fishermen. At present, as mentioned above, the most dominant species of cultivated Porphyra is P. yezoensis but the cultivation of P. tenera is restricted to certain culture-beds or the early half of the cultivation period. P. kunieda appears as a mixed species throughout most of the culture-beds, particulary in the later half of the period, while when it was picked in January it appeared dominantly in a place such as Gum-Dang where the 'Bal', splitted bamboo piece mat, was settled during the last of September. This is the first seeding process. The latter two species, P. suborbiculata and P. seriata appear frequently but in small amounts in the later half of the period particulary in the western region of the southern coast. However, it can not be ascertained when P. yezoensis becomes predominant, because specimens have not been available up until recent years but the process can be described as follows: We commonly recognize the ecological characteristics of P. tenera as follows; First, the conchospores of the species develop earlier and the period of its discharge is shorter than those of P. yezoensis; second, the microscopical buds discharge neutral spores which develop into new buds directly and buds develop repeatedly through a short period. Consequently, according to such above ecological characteristics, the species can grow thick on the 'Bal' exclusively. However, buds may disappear when they are harmed by disease such a 'infection by certain parasites or by other unusual environmental conditions. Thus P. yezoensis are enabled to grow on the 'Bal' instead of the former species since they not only develop later than the former but also macroscopical fronds discharge the neutral spore throughout the period from October to May. Likewise, if any disease appears in the culture-bed ill the later half of the period, the former is more severely damaged than the latter because the former have less resistance to the disease than the latter. Thus fewer frond survive and fewer carpospores which are the origin of the next generation can be discharged. However the latter by their nature can continue growing until early summer. In the case of the culture-bed where the above phenomenon occurs repeatedly P. yezoensis gradually may become the dominant species among cultivated Porphyra. In support of the validity of this process we find that according to the description and the plate of Wada (1941), P. tenera, P. yezoensis and P. kuniedai grow together in the culture-bed at the mouth of the Nakdong River where P. yezoensis occurs predominantly and mixed with P. kuniedai.

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