• 한국수산과학회지
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• 제43권2호
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• pp.139-145
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• 2010

This study investigated the optimum salinity and temperature conditions for mass culture of the brackish water flea, Diaphanosoma celebensis. Community and individual cultures of flea were maintained in 1 L beakers and 3 mL vessels (of a 12-well culture plate), respectively, and fed green algae, Tetraselmis suecica. In salinity experiments ranging from 5 to 34 psu, continuous growth of flea populations was found up to 34 psu. However, the specific growth rate and life span of females showed decreasing tendencies with the increase of salinity. The highest maximum density and offspring number were 33.6 individuals (ind.)/mL and 55.3 ind. at 10 psu, respectively. In the temperature experiments ranging from 20 to $40^{\circ}C$, population growth of D. celebensis increased continuously until $35^{\circ}C$ and then decreased over $40^{\circ}C$. The specific growth rate was significantly higher at 25 and $30^{\circ}C$ than at 20 and $40^{\circ}C$. Female life span tended to decrease with temperature increase. The highest maximum density and offspring number were 52.3 ind./mL and 46.0 ind. at $30^{\circ}C$, respectively. These results suggest that the optimum salinity and temperature for mass culture of D. celebensis may be 10 psu and $30^{\circ}C$, respectively.

• 한국양식학회지
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• 제10권2호
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• pp.199-211
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• 1997

톳의 유성생식을 통한 인공종묘생산 방법을 확립하기 위하여 초기 생장에 미치는 조도, 광주기 및 온도의 효과를 연구하였다. 각기 다른 조도 (500, 1,000, 2,000, 4,000, 8,000 lux), 광주기 (8 : 16, 12 : 12, 16 : 8 (L : D))와 온도 (10, 15, 20, 25, $30^{\circ}C$) 조건에서 유배를 70일간 배양한 결과 초기 생장 단계의 최적 조건으로는 조도 2,000-4,000 lux, 광주기 16 : 8 (L : D)와 온도조건으로는 15-2$0^{\circ}C$가 효과적인 것으로 나타났다.

• 공업화학
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• 제21권6호
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• pp.659-663
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• 2010

미세조류는 전 세계 바다에 분포하고 있으며 일부 종들은 인간의 식품에 이용되어 왔다. 특히, 광합성 미세조류 Nannochloropsis oculata는 영양적 가치가 우수하여 관심을 받고 있다. 본 연구에서는 광합성 미세조류 Nannochloropsis oculata의 고농도배양을 위한 배양온도, 초기 pH, 배양액 선정, 인공해수 농도, 배지농도, $CO_2$영향 등 최적조건을 확립하고자 하였다. 그 결과, 3%의 인공해수, 초기 pH 8.5, 배양온도 $25^{\circ}C$가 최적 배양조건으로 판별되었다. 미세조류에 $CO_2$를 공급하지 않았을 때에는 건조 균체량이 0.76 g/L이었지만, 5% $CO_2$ 공급 이후 1.50 g/L로 높은 성장률을 보였다. 클로로필 생합성은 미세조류 성장과 깊은 연관이 있는 것으로 판명되었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.267-273
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• 2004

The optimization of culture conditions for the bacterium Pseudomonas aeruginosa BYK-2 KCTC 18012P, was performed to increase its rhamnolipid production. The optimum level for carbon, nitrogen sources, temperature and pH, for rhamnolipid production in a flask, were identified as 25 g/L fish oil, 0.01% (w/v) urea, 25 and pH 7.0, respectively. Optimum conditions for batch culture, using a 7-L jar fermentor, were 200 rpm of agitation speed and a 2.0 L/min aeration rate. Under the optimum conditions, on fish oil for 216 h, the final cell and rhamnolipid concentrations were 5.3 g/L and 17.0 g/L respectively. Fed-batch fermentation, with different feeding conditions, was carried out in order to increase, cell growth and rhamnolipid production by the Pseudomonas aeruginosa, BYK-2 KCTC 18012P. When 2.5 g of fish oil and 100 mL basal salts medium, containing 0.01 % (w/v) urea, were fed intermittently during the fermentation, the final cell and rhamnolipid concentrations at 264 h, were 6.1 and 22.7 g/L respectively. The fed-batch culture resulted in a 1.2-fold increase in the dry cell mass and a 1.3-fold increase in rhamnolipid production, compared to the production of the batch culture. The rhamnolipid production-substrate conversion factor (0.75 g/g) was higher than that of the batch culture (0.68 g/g).

• International Journal of Computer Science & Network Security
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• 제22권10호
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• pp.207-213
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• 2022

The article reveals the problems of aesthetic education of young people as a necessary condition for the cultural development of the individual in the modern conditions of the information society. Aesthetic education should contribute to the formation of a creatively active personality. The basis of aesthetic education of young people in the modern conditions of the information society is the core of artistic culture - art as a unique form of public consciousness that contributes to the spiritual development and improvement of the inner world of a person. The main tasks of aesthetic education are highlighted. It is focused on the formation of aesthetic consciousness and aesthetic behavior of the individual. The formation of true aesthetic and spiritual values of students is impossible without a deep awareness of the national foundations of culture, which combines science (including technology), education, art, morality, way of life and worldview, and most importantly its information component - information culture. The effectiveness of aesthetic education of students largely depends on the skillful use of various methods and means by teachers. Aesthetic education of students involves a qualitative change in the level of their aesthetic culture in the modern conditions of the information society. In the era of information and computer technologies, the main Institute for aesthetic education of young people, as a necessary condition for the cultural development of the individual, is mass media. Television stands out especially because it has several information series (audio and video sequence), multiplied by the efficiency of providing information that increases several times compared to paper media, which allows you to report directly during the event.

• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.604-612
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• 2011

Beauveria bassiana SFB-205 supernatant can effectively control cotton aphid populations, which is closely associated with its chitinase activity. The present work extends to optimizing a culture medium to produce more efficacious supernatant in flask conditions, followed by scale-up in 7 L, 300 L and 1.2 KL fermentors with the parameter of chitinase. In flask conditions, a combination of soluble starch and yeast extract produced the greatest amount of chitinase (5.1 units/ml) and its supernatant had the highest aphicidal activity. An optimal quantitative combination of the two substrates, estimated by a response surface method, enabled the supernatant to have 15.7 units/ml of chitinase activity and 3.7 ml/l of median lethal concentration ($LC_{50}$) of toxicity against cotton aphid adults in laboratory conditions. In the scale-up conditions, overall supernatant had 25-28 units/ml of chitinase activity. Decrease in pH and limitation of dissolved oxygen (DO) during cultures were significantly related to the yield of chitinase. These results suggest that the substrate-dependent chitinase production can be background information for optimizing a culture medium, and pH and DO are critical factors in maximizing the production in scale-up conditions.

• 수산경영론집
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• 제15권1호
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• pp.96-113
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• 1984

It has been appeared since 1979 that the pattern of laver culture changes from household management to entrepreneurial management. The typical entrepreneurial laver culture management (ELCM) can be defined as a management by the floating aquaculturing method and mechanical processing system. Historical studies show that the ELCM could be operated only under the following condintions; \circled1 securing capital, labor and aquaculturing farm. \circled2 Leveling up of technology to combine these resources effectively. \circled3 Realizing its true value of products for good profits. In turning from household mangement to ELCM, there are some obstacles as follows ; \circled1 Uncertainty, which cannot maintein the merit of mass production because the object of laver culture is living in the sea. \circled2 Immaturity of management owing manly to the lower level of technology and scarcity of competent managers. \circled3 Social problems ; lack of aquaculturing farm, overpopulation in fishing villiage, side-job with agriculture etc. For these reasons, there has been household management with overlabor and underconsumption in fishing village. In order that ELCM can enter the stage of maturity, those problems should be solved. Because ef laver industrial inferiority, these can be solved by the support assistance of government. As the required conditions in ELCM are filled by the government policy, ELCM are increased recently in its numbers.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2010년도 춘계 종합학술대회 논문집
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• pp.112-114
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• 2010

Mainly through the pervasive emergence of portable devices (laptops, PDAs, now iPods), the screen arts, that is, the cinematic arts has suffered the loss of what constitutes the core of its existence: the theatre and theatre-audience. This paper discusses cinema's distinct place and significance in culture and its difference from television and other forms of mass media. The most pressing question at hand would be, "how do you bring back attention to the medium that is distinctly cinematic?" "How do you bring people back to the theatres?" This paper argues that cinema, and the film industry as a whole must push for a technological leap that is in conjunction with culture. With mentioning of the rapid development of 3D technologies in the moving images, this paper proposes that such technological novelties and innovation are necessary conditions for a new kind of film culture to emerge.

• 대한수의학회지
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• 제33권2호
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• pp.277-283
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• 1993

In the present study, observations were made on the life-history of Lymnaea viridis under laboratory conditions, involving incubation period of the eggs and their hatching rate, shell length of the newly hatched snails, sexual maturity, size of the snails when the snail produced the first egg-mass, the number of eggs in each egg-mass, egg-laying, ovipostion, growth rate of the snails, and longevity of the snail. At temperatures between $19.8^{\circ}C$ to $22.5^{\circ}C$, incubation period of the eggs occupied 10~12 days, and after beginning of hatching, all young snails emerged completely from the egg-mass within 5 days. The hatching rate was 88%. The average shell length of the newly hatched snails was about 0.064cm. The rate of growth was extraordinarily rapid under good laboratory conditions. When two snails were reared in one culture vessel($20{\times}15{\times}5cm$) with blue-green algae at about $22^{\circ}C$, snail growth was optimal, taking 37 days to reach 1.2cm in shell length. Sexual maturity reached in about 19 days. The size of the snails at sexual maturity was $0.78{\pm}0.05cm$ in length and $0.47{\pm}0.04cm$ in width. The first egg-masses produced were $0.59{\pm}0.22cm$ in length and $0.34{\pm}0.08cm$ in width, and contained 7~38 eggs. The eggs are usually laid in water. The egg-laying was affected by food and temperature. Snails fed with blue-green algae at about $22^{\circ}C$ produced larger egg-masses than the snails fed with fish food at about $26^{\circ}C$. Under conditions of continuous activity and growth, the maximum expectation of life appears to be 109~350(mean 230) days. And the shell length of snails at death were 1.39~1.64cm.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.523-530
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• 1998

Rapid and quantitative analysis of physiological change and clavulanic acid production was studied by the combination of pyrolysis mass spectrometry (PyMS) and artificial neural network (ANN) in Streptomyces clavuligerus. Firstly, the continuous culture studies were carried out to get the physiological background and PyMS samples. Clavulanic acid production was inversely related to growth rate: Mycelium growth and $q_{cal}$ were optimum at 0.1 $h^{-1}\; and \;0.025 h^{-1}$ respectively. Changes in specific nutrient uptake rates ($q_{gly}$ and $q_{amn}$) also affected clavulanic acid production since clavulanic acid production appeared to be stimulated by the limitation of carbon and nitrogen. Fermentation broth containing mycelium taken from continuous cultures was analyzed by PyMS, and the PyMS spectra were analyzed with multivariate statistics. PCCV plots revealed that samples harvested under the same culture condition were clustered together but samples from different culture conditions formed separate clusters. To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the concentration of clavulanic acid, ANN was trained on Py MS data using a radial basis function classifier. The results showed that the physiological stages with different growth rate were successfully differentiated and it was possible to monitor the clavulanic acid production precisely and rapidly.