• 제목/요약/키워드: Mass culture conditions

검색결과 280건 처리시간 0.036초

Fusarium proliferatum KGL0401 as a New Gibberellin-Producing Fungus

  • Rim, Soon-Ok;Lee, Jin-Hyung;Choi, Wha-Youl;Hwang, Seon-Kap;Seok, Jong-Suh;Lee, In-Joong;Rhee, In-Koo;Kim, Jong-Guk
    • Journal of Microbiology and Biotechnology
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    • 제15권4호
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    • pp.809-814
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    • 2005
  • Gibberellins (GAs) play an important role in plant growth and development. Fifteen fungi were isolated from Physalis alkekengi var francheti plant roots, and among them, four isolates showed GA-production activity. A bioassay using waito-c rice was carried out with the culture fluid of the GA-producing fungi. The GA-producing fungi were cultured for 7 days in Czapek's liquid medium at $30^{\circ}C$, 120 rpm, under dark conditions. The culture broth was concentrated 30-fold and 10 ${\mu}l$ of that concentrate was applied to 2-leaf rice sprouts. The height of the rice seedlings treated with the culture fluid of isolate PA08 was 26 cm high, while that of the seedlings treated with the wild-type Gibberella fujikuroi was 13 cm high. As such, the plant growth-promoting activity exhibited by isolate PA08 was 2 times stronger than that exhibited by the wild-type G fujikuroi. The amounts of $GA_l,\;GA_3,\;GA_4,\;GA_7,\;GA_9,\;GA_{20}$, and $GA_{24}$ in the medium were measured using gas chromatography-mass spectrometry (GC-MS), and the quantities produced by isolate PA08 were 4.85 ng/ml, 4.79 ng/ml, 17.30 ng/ml, 6.01 ng/ml, 16.61 ng/ ml, 0.08 ng/ml, and 17.30 ng/ml, respectively. Isolate PA08 was also identified as Fusarium proliferatum KGL0401 by a genetic analysis of the nucleotide sequences of the internal transcribed spacer region of the ribosomal DNA.

Optimal Production Conditions of Streptomyces griseus Trypsin (SGT) in Streptomyces lividans

  • Koo, Bon-Joon;Kim, Joung-Mee;Byun, Si-Myong;Hong, Soon-Kwang
    • BMB Reports
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    • 제32권1호
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    • pp.86-91
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    • 1999
  • The sprT gene encoding Streptomyces griseus trypsin (SGT) was introduced into Streptomyces lividans TK24 and Streptomyces lividans 1326 to study which strain would be better to overexpress the extracellular proteinase. Various media with different compositions were also used to maximize the productivity of SGT in heterologous hosts. The SGT productivity was best when the transformants of S. lividans TK24 and 1326 were cultivated in R2YE medium, and their relative trypsin activity of the culture broth measured with an artificial chromogenic substrate, N-${\alpha}$-benzoyl-DL-arginine-${\rho}$-nitroanilide, were 382 units/ml and 221 units/ml, respectively. They produced high levels of SGT in GYE medium but relatively lower than those in R2YE medium, and negligible amount of SGT was produced in Ferm, RASF, LIVID, and NDSK media. Considering non-SGT associated activity in Pronase powder, it was estimated that the transformant of S. lividans TK24 can produce SGT in R2YE 3.5 times more than the amount by S. griseus 10137 from which the sprT gene had been originated. The growth of S. lividans reached the maximum level of cell mass at 5 d of culture, but SGT production started in the stationary phase of cell growth and kept increasing until the ninth day of culture in R2YE medium, but in GYE media the productivity reached at the maximum level at 7 d of cultivation.

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매일신문에 나타난 복식현상에 관한 연구(I)-남성복, 여성복, 아동복 중심으로- (Study on the aspect of costume in "Maeil Shinmun" - Focused on Men's wear, Women's wear and Children's wear-)

  • 은영자;최윤혜;형승희
    • 복식
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    • 제37권
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    • pp.133-148
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    • 1998
  • This study presents the aspects and changing process of the habiliment from the National Liberation up to now by analyzing the articles of costume found in$\ulcorner$Maeil Shinmun$\lrcorner$from Jan. 1. 1946 to Dec. 31. 1996. In the period of 1946~1959, the political and economic conditions through the National Liberation and Korean war deteriorated and the interest in costumes were weakened. But the economic recovery and social stability in the middle of the 50's made the lost interest in costumes increase. From 1960 to 1969, the high economic growth was achieved by“Five year plans for Economic Development”and the progress of mass media, transportation, communication and education brought western culture to our society. It spread rapidly and changed the way of life and the sence of value. These phenomenon led to a great transition on the Korean costume culture in the 60's In 1970~1979, the articless of costume made a change from the 60's, which focused on women's wear, and tuned to one including general costumes not only women's wear but also men's wear due to the women's social roles, the unisexmode and diverse costume. In 1980~1989, the growth of the consumption, the banning of the school uniform, Asian Games(1986) and Olympic games(1988) caused the high qualification, personalization and diversification in the fashion industry. Further more, the traditional-oriented trend with korean culture was emphasized. It pursued the modernization of the traditional Korean Costume, Hanbok, to introduce the unique Korean Beauty. From 1990 to 1996, in the era of international-ization and open door policy, the competition of nations became intense and the comprehention about environmental destruction highlingted the concerns on ecology. The recognition of environmental protection and recycling were reflected in the fashion. It prevailed that the pursuit of personalization,. diverse style, practicality and the fashion leader called generation appeared.

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Higher Biomass Productivity of Microalgae in an Attached Growth System, Using Wastewater

  • Lee, Seung-Hoon;Oh, Hee-Mock;Jo, Beom-Ho;Lee, Sang-A;Shin, Sang-Yoon;Kim, Hee-Sik;Lee, Sang-Hyup;Ahn, Chi-Yong
    • Journal of Microbiology and Biotechnology
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    • 제24권11호
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    • pp.1566-1573
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    • 2014
  • Although most algae cultivation systems are operated in suspended culture, an attached growth system can offer several advantages over suspended systems. Algal cultivation becomes light-limited as the microalgal concentration increases in the suspended system; on the other hand, sunlight penetrates deeper and stronger in attached systems owing to the more transparent water. Such higher availability of sunlight makes it possible to operate a raceway pond deeper than usual, resulting in a higher areal productivity. The attached system achieved 2.8-times higher biomass productivity and total lipid productivity of $9.1g\;m^{-2}day^{-1}$ and $1.9g\;m^{-2}day^{-1}$, respectively, than the suspended system. Biomass productivity can be further increased by optimization of the culture conditions. Moreover, algal biomass harvesting and dewatering were made simpler and cheaper in attached systems, because mesh-type substrates with attached microalgae were easily removed from the culture and the remaining treated wastewater could be discharged directly. When the algal biomass was dewatered using natural sunlight, the palmitic acid (C16:0) content increased by 16% compared with the freeze-drying method. There was no great difference in other fatty acid composition. Therefore, the attached system for algal cultivation is a promising cultivation system for mass biodiesel production.

천연제오라이트 세라믹볼을 이용한 곤충병원성 곰팡이 포자 생산 방법 (Culture Method of Spore for Entomopathogenic Fungus Using Natural Zeolite Ceramic Ball)

  • 이중복;김범수;주우홍;권기석
    • 한국환경농학회지
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    • 제35권1호
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    • pp.72-78
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    • 2016
  • 본 곤충병원성곰팡이 B. bassiana M130의 포자를 대량생산하기 위한 방안으로 천연제오라이트 세라믹볼, 합성제오라이트 세라믹볼과 활성탄볼을 이용한 담체배양을 실시하였다. 그 중 천연제오라이트 세라믹볼 이용한 포자생산방법이 가장 우수 한 것으로 조사 되었으며, 배양 최적조건은 미강추출물(1:8, W/V) 3 mL, 전분 33 g, 포자현탁액 7 mL, pH3과 NaCl 5%로 배양하였을 때 B. bassiana M130 포자를 4.2×108 conidium/mL 생산하고, 사이클론 회수방법을 통해 순수포자만 63%의 회수율을 보였다. 향후 천연제오라이트 세라믹볼을 이용한 곤충병원성 곰팡이 B. bassiana M130 포자의 대량생산이 가능하며, 온실가루이제어용 친환경살충제로 제품생산 및 원제의 대량생산을 통해 국산화 가능성을 제안하고자 한다.

Differential Effects of Fumonisin $B_1$ on Cell Death in Cultured Cells: the Significance of the Elevated Sphinganine

  • Yu, Chang-Hun;Lee, Yong-Moon;Yun, Yeo-Pyo;Yoo, Hwan-Soo
    • Archives of Pharmacal Research
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    • 제24권2호
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    • pp.136-143
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    • 2001
  • Fumonisins are specific inhibitors of ceramide synthase in sphingolipid metabolism. An alteration in sphingolipid metabolism as a result of fumonisin exposure is related to cell death (Yoo et al., 1992). The objective of this study was to investigate whether elevated free sphinganine levels are related to the sensitivity of cultured cells to fumonisin exposure. Fumonisin $B_1$ elevated the intracellular free sphinganine concentraions in both LLC-$PK_1$ and Chinese hamster ovary (CHO) cells. However, CHO cells are resistant to fumonisin cytotoxicity at 50${u}m$, while LLC-$PK_1$ cells are sensitive at concentrations greater than 357M. The intracellular concentration of free sphinganine in LLC-$PK_1$ cells treated at 50${u}m$ fumonisin $B_1$ for 72 h was approximately 1450 pmol/mg protein relative to the 37 pmol observed in the control culture. Under the same conditions, the population of apoptotic cells in the 50${u}m$ fumonisin $B_1$-treated culture was approximately 37% of the total compared to 12% in the control. The caspase III-like activity after 72 h in the 50${\mu}$M fumonisin $B_1$-exposed culture Increased to approximately 50 $pmol/mg$ protein/hr compared to 6 $pmol/mg$ protein/hr in the control. L-cycloserine, a serine palmitoyltransferase inhibitory reduced the fumonisin $B_1$-stimulated caspase III-like activity down to the control level. Under the same culture conditions, the intracellular concentration of free sphinganine after-cycloserine plus fumonisin $B_1$ treatment was 140 pmol/mg protein compared to 1450 $pmol/mg$ protein in fumonisin $B_1$ alone. The intracellular concentration of free sphinganine in CHO cells treated with 50${u}m$ fumonisin $B_1$ for 72 h was al)proximately 460 pmol/mg protein, indicating that the mass amount of elevated free sphinganine in the CHO cells was about 32% of that in LLC-$PK_1$ cells. Adding exogenous sphinganine to the CHO cells along with 50${u}m$ fumonisin $B_1$ treatment for 72 h caused both necrosis and apoptosis. In conclusion, the elevated endogenous sphinganine acts as a contributing factor to the fumonisin-induced cell death.

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Methylobacterium organophilum에 의한 메탄올로부터 고점도 다당류, 메틸란 생산을 위한 배양조건 최적화 (Optimization of Culture Conditions for Production of a High Viscosity Polysaccharide, Methylan, by Methylobacterium organophilum from Methanol.)

  • 최준호;이운택;김상용;오덕근;김정회
    • 한국미생물·생명공학회지
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    • 제26권3호
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    • pp.244-249
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    • 1998
  • Methylobacterium organophilum을 사용하여 메탄올로부터 세포외 고점도 다당류, 메틸란의 생산을 특정 배양조건에서 수행하였다. C/N 비율이 미치는 영향을 살펴본 결과 균체의 비성장속도는 C/N 비율에 대하여 큰 차이가 없었고, 단위 균체당 메틸란 생산수율은 C/N 비율 약 30부근에서 최적이었다. 여러 가지 금속염 중에서 Mn$^{++}$과 Fe$^{++}$ 이온이 결핍하면 메틸란의 생산이 현저히 저해됨을 보였다. 균체 농도와 메틸란 생산은 pH는 7근처에서 최적을 나타내었다. 균체 성장의 최적온도는 37$^{\circ}C$이었으나 메틸란 생산의 최적 온도는 3$0^{\circ}C$이었다. 메탄을 농도가 증가할수록 비성장속도가 감소하였고, 4% 이상의 농도에서는 균체 성장이 완전히 저해되었다. 메틸란 생산을 위한 최적 메탄올 농도는 초기농도로 0.5%(v/v)이었고 그 이상의 농도에서는 메틸란 생산이 급격히 감소하였다. 메탄올의 독성과저해를 극복하기 위하여 최적 배양조건에서 메탄올의 유가식 배양을 수행하여 최종 균체 농도와 최종 메틸란 농도가 각각 12.4 g/l 및 8.7 g/l이었다.

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Pilot Scale의 박테리아 셀룰로오스 생산 및 그의 물성 (Production of Bacterial Cellulose by Pilot Scale and Its Properties)

  • 김성준;송효정;장미화;최창남
    • KSBB Journal
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    • 제22권2호
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    • pp.91-96
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    • 2007
  • 본 연구에서는 음식폐기물 효소당화액을 BC생산 배지원으로 이용하여, flask정치배양과 50 L로 규모확대한 공기순환배양기에서 BC생산성을 검토하고, 생산된 BC의 제반물성을 조사하였다. 전단력을 낮추기 위해 당화액배지에 agar를 첨가하여 10 L, 50 L 공기순환배양기에서 배양한 결과, 50 L 규모로 scale-up한 반응기에서 5.64 g/L로 10 L 공기순환배양기 (5.84 g/L)에서와 동등한 BC 농도가 얻어져 BC 대량생산에 효과적인 배양방법임이 입증되었다. 50 L 규모로 확대된 공기순환배양기를 이용하는 본 배양 방법은 BC의 저비용 대량생산을 위한 효율적인 생산 방법 중 하나가 되리라 사료된다. 또한, 당화액을 배지로 하여 공기순환 방법으로 제조한 BC의 물성을 정치배양 방법으로 제조한 BC의 물성과 비교 검토한 결과, 공기순환법으로 제조하였을 때 중합도 및 역학적 성질이 약간 저하하였으나 결정성은 비슷하였으며, 배양기 용량의 대소에 따른 물성의 변화는 거의 없었다.

태교 실천에 대한 일상생활 기술적 연구 (An Ethnographic Study about Taegyo Practice in Korea)

  • 김현옥
    • 대한간호학회지
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    • 제27권2호
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    • pp.411-422
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    • 1997
  • The purpose of this study is twofold : (i) to investigate how much effort the married couples are making for the good health of both the pregnant woman and her unborn child from the time of their marriage to and during the period of conception : and (ii) to comprehensive investigate socio-cultural back-grounds which affect prenatal effort. Result of this study provide a basis for the prenatal care program which will be appropriate to our culture. This study has been done by the ethnographic research method. The subjects of this study are 53 people in all consisting of 33 pregnant women and 20 husbands. In order to investigate socio-cultural factors which influence Taegyo, producers of Taegyo music were interviewed. In addition the researcher surveyed the markets of Taegyo music, participated in special courses of prenatal education, analyzed the content of the books and periodicals dealing with Taegyo, and collected the concept of Taegyo distributed by the mass media. The full-fledged study continued for eight months from February to August.1996. The data were analyzed as soon as they were collected. Spradly's(1979, 1980) developmental, sequential method of domain analysis. taxonomic analysis, componential analysis, and theme analysis in this order was adopted as the procedure of analyzing the data. To obtain the exactness of study, Sandelowski's (1986) four criteria, that is, Credibility, Fittingness, Auditability, and Confirmability were applied to all stages of data collection, data analysis, the interpretation of the result, and the description of the result. The following are the result : 1. The couples' Taegyo at the stage of preconception was related to their physical, psychological, spiritual conditions under which a healthy baby will be born. Specific methods they prefer are : "the choice of one's spouse." "physical check-up," "physical good health, " "praying, " and so on. 2. When the marriod couple have sex in order to conceive, their Taegyo was related to the imposition of their physical, psychological, and environmental conditions. Specific methods they prefer are : "having sex at specific time, " "having sex in nice place." "to purify their minds while having sex," and so on. 3. The married couples' Taegyo while they are in pregnancy was related to the imposition of their physical. psychological, emotionmental. environmental, social and spiritual conditions. Specific methods they prefer are : "listening to music. " "reading," "looking at beautiful things only," "to avoid looking at or listening to bad things." "to eat food in good shape, " "to avoid drugs," "eating Korean herbal medicine." "sexual abstinence," "to avoid dangerous places," "to keep emotional tranquility," "moderate exercises and rest." "leading a pure life." "praying." "being aware of their words and behavior." "for the couple to keep a good relationship." "interaction with their unborn child," "to support Taegyo for pregnant women," and so on. 4. The married couple put Taegyo into practice on the basis of the following principles : the principle of respecting an unborn child, the principle of forming a good disposition. the principle of top-down parental love, the principle of synergy between a pregnant woman and her unborn child, the principle of expecting a good child, the principle of forming a good habit, and the principle of acquiring a parental role. 5. The practice of Taegyo is influenced by such factors as the married couple, the supporting system, and the mass media. As the husband -and-wife factor, their information of Taegyo, the degree of importance is assigned to their characters, their time to spare, their healthiness, the age of pregnant woman, their conception plan, their religion, their belief of the Taegyo effects, and the birth of a baby in this order. The factor of the supporting system consists of her husband's support, her family support, and her neighbor's support. The mass media factors include the broadcasting media, books specialized in Taegyo, periodicals for pregnant women, booklets for advertizing powdered milk, Taegyo music of record manufacturing companies, and the teaching materials for gifted children. Among these the mass media is especially taking advantage of Taegyo as its main source of economic profits are leading the public behavior pattern to a prodigal one. Taegyo is a self-control behavior which requires practice for the following : the physical and psychological good health of the pregnant woman and her unborn child, the development of the unborn child's good character, the development of the unborn child's intelligence and talents, the expectation of the unborn child's good features. shape a good habit, the expectation of the unborn child's bright future, and the learning of a parental role, the expectation of male birth. Above all it is a type of our good cultural tradition which pursues a value higher than the one that the prenatal care does. The principles of pregnancy care inherent in the habit of Taegyo will provide us a guideline for the development of the prenatal care.

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생장조절제가 하월시아 만상(Haworthia maughanii)의 기내 대량증식에 미치는 영향 (Effect of growth regulators on In Vitro mass propagation of Haworthia maughanii)

  • 김윤희;김혜형;이지영;이재홍;정재홍;;이상덕
    • Journal of Plant Biotechnology
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    • 제45권4호
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    • pp.369-374
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    • 2018
  • 하월시아(Haworthia maughanii)의 기내 식물체 대량생산을 위하여 캘러스 유도에 적합한 부위와 신초 및 발근에 영향을 미치는 생장조절제 종류 및 농도를 구명하고자 본 시험을 수행하였다. 캘러스 유도에 적합한 식물 부위를 구명 하기 위해 잎, 화뢰, 화경을 이용하여 NAA와 TDZ의 농도를 달리하여 MS배지에 치상한 결과, 잎은 $NAA\;1mgL^{-1}$$TDZ\;1mgL^{-1}$, 화뢰는 $NAA\;2mgL^{-1}$$TDZ\;2mgL^{-1}$, 화경는 $NAA\;1mgL^{-1}$이상의 단용처리에서 100% 캘러스 형성율을 보였고, 모든 부위에서 캘러스 형성율은 높았으나 가장 많은 배양절편 확보가 가능한 잎이 가장 효율적으로 판단되었다. $NAA\;0.1mgL^{-1}$$BA\;0.1mgL^{-1}$ 혼용처리구에서 다른 처리에 비해서 신초가 22.0개로 신초 발생에 가장 효과적이었다. 또한 신초의 대량증식은 $NAA\;0.1mgL^{-1}$$BA\;0.1mgL^{-1}$처리구에서 신초가 16.3개로 가장 많이 형성되었다. 이러한 결과는 최적의 기내 배양 조건을 통해 하월시아 만상의 대량생산 가능성을 보여주었다.