• Title/Summary/Keyword: Mass Spectrum

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Separating of Falcarinol from Acanthopanax senticosus (가시오가피(Acanthopanax senticosus)에서 polyacetylene계 화합물인 falcarinol의 동정)

  • Kim, Seong-Ju;Chang, Kyu-Seob
    • Korean Journal of Food Science and Technology
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    • v.37 no.3
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    • pp.360-363
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    • 2005
  • To detect falcarinol in Acanthopanax senticosus, its stem and bark were extracted with methanol fractionated with petroleum ether and diethyl ether, and separated by silica gel column chromatography, Resulting six crude samples were compared with standard falcarinol through TLC on silica gel plates. Fraction 6 showed $R_{f}$ value of 0.46 similar to that of standard falcarinol. Through analytical reverse phase HPLC/PDA, UV spectra of standard falcarinol and traction 6 recorded between 200 and 340 nm showed identical peaks and UV spectra patterns. GC/MS revealed standard falcarinol and fraction 6 have equal retention times of 7.4 and 8.5 min before and after TMS-derivatization, respectively. Standard falcarinol and fraction 6 showed more similar spectrum patterns after TMS-derivatization than before. These results confirm presence of falcarinol in A. senticosus.

Estimation and Analysis of Wave Spectrum Parameter using HeMOSU-2 Observation Data (HeMOSU-2 관측 자료를 이용한 파랑 스펙트럼 매개변수 추정 및 분석)

  • Lee, Uk-Jae;Ko, Dong-Hui;Kim, Ji-Young;Cho, Hong-Yeon
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.33 no.6
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    • pp.217-225
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    • 2021
  • In this study, wave spectrum data were calculated using the water surface elevation data observed at 5Hz intervals from the HeMOSU-2 meteorological tower installed on the west coast of Korea, and wave parameters were estimated using wave spectrum data. For all significant wave height ranges, the peak enhancement parameter (γopt) of the JONSWAP spectrum and the scale parameter (α) and shape parameter (β) of the modify BM spectrum were estimated based on the observed spectrum, and the distribution of each parameter was confirmed. As a result of the analysis, the peak enhancement parameter (γopt) of the JONSWAP spectrum was calculated to be 1.27, which is very low compared to the previously proposed 3.3. And in the range of all significant wave heights, the distribution of the peak enhancement parameter (γopt) was shown as a combined distribution of probability mass function (PMF) and probability density function (PDF). In addition, the scale parameter (α) and shape parameter (β) of the modify BM spectrum were estimated to be [0.245, -1.278], which are lower than the existing [0.300, -1.098], and the result of the linear correlation analysis between the two parameters was β = -3.86α.

Proteome Data Analysis of Hairy Root of Panax ginseng : Use of Expressed Sequence Tag Data of Ginseng for the Protein Identification (인삼 모상근 프로테옴 데이터 분석 : 인삼 EST database와의 통합 분석에 의한 단백질 동정)

  • Kwon, Kyung-Hoon;Kim, Seung-Il;Kim, Kyung-Wook;Kim, Eun-A;Cho, Kun;Kim, Jin-Young;Kim, Young-Hwan;Yang, Deok-Chun;Hur, Cheol-Goo;Yoo, Jong-Shin;Park, Young-Mok
    • Journal of Plant Biotechnology
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    • v.29 no.3
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    • pp.161-170
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    • 2002
  • For the hairy root of Panax ginseng, we have got mass spectrums from MALDI/TOF/MS analysis and Tandem mass spectrums from ESI/Q-TOF/MS analysis. While mass spectrum provides the molecular weights of peptide fragments digested by protease such as trypsin, tandem mass spectrum produces amino acid sequence of digested peptides. Each amino acid sequences can be a query sequence in BLAST search to identify proteins. For the specimens of animals or plants of which genome sequences were known, we can easily identify expressed proteins from mass spectrums with high accuracy. However, for the other specimens such as ginseng, it is difficult to identify proteins with accuracy since all the protein sequences are not available yet. Here we compared the mass spectrums and the peptide amino acid sequences with ginseng expressed sequence tag (EST) DB. The matched EST sequence was used as a query in BLAST search for protein identification. They could offer the correct protein information by the sequence alignment with EST sequences. 90% of peptide sequences of ESI/Q-TOF/MS are matched with EST sequences. Comparing 68% matches of the same sequences with the nr database of NCBI, we got more matches by 22% from ginseng EST sequence search. In case of peptide mass fingerprinting from MALDI/TOF/MS, only about 19% (9 proteins of 47 spots) among peptide matches from nr DB were correlated with ginseng EST DB. From these results, we suggest that amino acid sequencing using tandem mass spectrum analysis may be necessary for protein identification in ginseng proteome analysis.

Refinement of GRACE Gravity Model Including Earth's Mean Mass Variations (지구 평균 질량 변화를 포함한 GRACE 중력 모델 보정)

  • Seo, Ki-Weon;Eom, Jooyoung;Kwon, Byung-Doo
    • Journal of the Korean earth science society
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    • v.35 no.7
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    • pp.537-542
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    • 2014
  • The Gravity Recovery and Climate Experiment (GRACE) has observed the Earth's mass redistribution mainly caused by the variations of groundwater, ice sheet, and sea level since its launch in April 2002. The global gravity model estimated by the GRACE observation is corrected by barometric pressure, and thus represents the change of Earth mass on the Earth's surface and below Earth's surface excluding air mass. However, the total air mass varies due to the water exchange between the Earth's surface and the atmosphere. As a result, the nominal GRACE gravity model should include the Earth's gravity spectrum associated with the total air mass variations, degree 0 and order 0 coefficients of spherical harmonics ($C_{00}$). Because the water vapor content varies mainly on a seasonal time scale, a change of $C_{00}$ (${\delta}C_{00}$) is particularly important to seasonal variations of sea level, and mass balance between northern and southern hemisphere. This result implies that ${\delta}C_{00}$ coefficients should be accounted for the examination of continental scale mass change possibly associated with the climate variations.

SPHERICAL WIND ACCRETION ONTO SUPERMASSIVE BLACK HOLE (우리은하 중심의 초거대 질량 블랙홀에 대한 구형 항성풍 부착)

  • Im, Su-Yeon;Park, Myeong-Gu
    • Publications of The Korean Astronomical Society
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    • v.10 no.1
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    • pp.79-90
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    • 1995
  • The unique compact radio source, Sgr $A^*$, at the Galactic center show many observational signs that it is powered by supermassive black hole. Recent observations also imply that it is surrounded by winds from nearby IR sources. So we explore the model in which multiwavelength spectrum from Sgr $A^*$ is due to the spherical accretion of these winds onto the central supermassive black hole. Improving upon the previous work, we allowed the possibility that ions and electrons have different temperatures, included the Compton effects and pair processes. Electrons radiate via cyclosynchrotron and bresstrahlung with comptoniztion. We find that ion approaches the virial temperature ${\sim}10^{13}K$ while electron temperature saturates at ${\sim}10^{10}K$. However, decoupling between ion and electron does not greatly affect the shape of the emission spectrum. When the mass of the black hole is ${\sim}10^6M_{\odot}$, radio, IR, X-ray, $\gamma$-ray band spectrum is reasonably explained by the model. Yet Compton effect which is neglected in previous works produces significant emission in IR band, which is marginally compatible with observations. Pair production is negligible and annihilation lines cannot be observed.

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Response Spectra of Structure Installed Frictional Damping System (마찰형 감쇠를 갖는 구조물의 응답 스펙트럼)

  • Park, Ji-Hun;Youn, Kyong-Jo;Min, Kyung-Won;Lee, Sang-Hyun
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.11a
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    • pp.893-897
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    • 2006
  • Structures with additional frictional damping system have strong nonlinearity that the dynamic behavior is highly affected. by the relative magnitude between frictional force and excitation load. In this study, normalized response spectra of the structures with non-dimensional friction force are obtained through nonlinear time history analyses of the mass-normalized single degree of freedom systems using 20 ground motion data recorded on rock site. The variation of the control performance of frictional damping system is investigated in terms of the dynamic load and the structural natural period, of which effects were not considered in the previous studies. Least square curve fitting equations are presented for describing those normalized response spectrum and optimal non-dimensional friction forces are obtained for controlling the peak displacement and absolute acceleration of the structure based on the derivative of the curve fitted design spectrum.

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Response of double hinged articulated tower platforms to wind forces

  • Islam, Nazrul;Zaheer, Mohd Moonis;Ahmed, Suhail
    • Wind and Structures
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    • v.12 no.2
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    • pp.103-120
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    • 2009
  • Articulated tower platforms due to its compliant nature are more susceptible to the dynamic effects of wind than conventional fixed platforms. Dynamic response analysis of a double hinged articulated tower excited by low frequency wind forces with random waves is presented in this paper. The exposed super structure of the platform, housing the drilling and production facilities is subjected to mean and fluctuating wind loads, while the submerged portion is acted upon by wind driven waves. The fluctuating component of the wind velocity is modeled by Emil Simiu's spectrum, while the sea state is characterized by Pierson-Moskowitz spectrum. Nonlinearities in the system due to drag force, added mass, variable submergence and instantaneous tower orientation are considered in the analysis. To account for these nonlinearities, an implicit time integration scheme (Newmark's-${\beta}$) has been employed which solves the equation of motion in an iterative fashion and response time histories are obtained. The power spectra obtained from random response time histories show the significance of low frequency responses.

An Analysis of Characteristics of Floor Dynamic Properties and Bang-machine Impact Force on Floating Floor Using System Analysis (시스템 해석을 이용한 뜬바닥구조에서의 바닥구조 동특성과 뱅머신의 충격력 특성 분석)

  • Mun, Dae-Ho;Park, Hong-Gun;Hwang, Jae-Seung;Hong, Geon-Ho
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.24 no.5
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    • pp.390-398
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    • 2014
  • Heavy-weight floor impact noise is directly related to the impact source and floor vibration property. Dynamic properties of the standard floating floor that is used in Korea was investigated using accelerance, acceleration energy spectral density(ESD), and structural modal test. In the standard floating floor, natural frequency was decreased by the finishing mortar mass and the damping ratio was increased. Bang-machine force spectrum acting on the concrete slab can be calculated using inverse system analysis. Impact force acting on concrete slab is changed by interaction of finishing mortar and resilient material. The amplitude of the bang-machine force spectrum was amplified in low frequency range(below 100 Hz), and over 100 Hz was decreased. Changed force spectrum influence to the response of structure vibration, so the heavy-weight floor impact noise level was changed.

Response Spectra of Structure Installed Frictional Damping System (마찰형 감쇠를 갖는 구조물의 응답 스펙트럼)

  • Park, Ji-Hun;Youn, Kyung-Jo;Min, Kyung-Won;Lee, Sang-Hyun
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.17 no.1 s.118
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    • pp.88-94
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    • 2007
  • Structures with additional frictional damping system have strong nonlinearity that the dynamic behavior is highly affected by the relative magnitude between frictional force and excitation load. In this study, normalized response spectra of the structures with non-dimensional friction force are obtained through nonlinear time history analyses of the mass-normalized single degree of freedom systems using 20 ground motion data recorded on rock site. The variation of the control performance of frictional damping system is investigated in terms of the dynamic load and the structural natural period, of which effects were not considered in the previous studies. Least square curve fitting equations are presented for describing those normalized response spectrum and optimal non-dimensional friction forces are obtained for controlling the peak displacement and absolute acceleration of the structure based on the derivative of the curve-fitted design spectrum.

A PROCEDURE FOR GENERATING IN-CABINET RESPONSE SPECTRA BASED ON STATE-SPACE MODEL IDENTIFICATION BY IMPACT TESTING

  • Cho, Sung-Gook;Cui, Jintao;Kim, Doo-Kie
    • Nuclear Engineering and Technology
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    • v.43 no.6
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    • pp.573-582
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    • 2011
  • The in-cabinet response spectrum is used to define the input motion in the seismic qualification of instruments and devices mounted inside an electrical cabinet. This paper presents a procedure for generating the in-cabinet response spectrum for electrical equipment based on in-situ testing by an impact hammer. The proposed procedure includes an algorithm to build the relationship between the impact forces and the measured acceleration responses of cabinet structures by estimating the state-space model. This model is used to predict seismic responses to the equivalent earthquake forces. Three types of structural model are analyzed for numerical verification of the proposed method. A comparison of predicted and simulated response spectra shows good convergence, demonstrating the potential of the proposed method to predict the response spectra for real cabinet structures using vibration tests. The presented procedure eliminates the uncertainty associated with constructing an analytical model of the electrical cabinet, which has complex mass distribution and stiffness.