• Interdisciplinary Bio Central
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• v.1 no.3
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• pp.9.1-9.6
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• 2009

Introduction: In the mass spectrometry-based proteomics, biological samples are analyzed to identify proteins by mass spectrometer and database search. Database search is the process to select the best matches to the experimental mass spectra among the amino acid sequence database and we identify the protein as the matched sequence. The match score is defined to find the matches from the database and declare the highest scored hit as the most probable protein. According to the score definition, search result varies. In this study, the difference among search results of different search engines or different databases was investigated, in order to suggest a better way to identify more proteins with higher reliability. Materials and Methods: The protein extract of human mesenchymal stem cell was separated by several bands by one-dimensional electrophorysis. One-dimensional gel was excised one by one, digested by trypsin and analyzed by a mass spectrometer, FT LTQ. The tandem mass (MS/MS) spectra of peptide ions were applied to the database search of X!Tandem, Mascot and Sequest search engines with IPI human database and SwissProt database. The search result was filtered by several threshold probability values of the Trans-Proteomic Pipeline (TPP) of the Institute for Systems Biology. The analysis of the output which was generated from TPP was performed. Results and Discussion: For each MS/MS spectrum, the peptide sequences which were identified from different conditions such as search engines, threshold probability, and sequence database were compared. The main difference of peptide identification at high threshold probability was caused by not the difference of sequence database but the difference of the score. As the threshold probability decreases, the missed peptides appeared. Conversely, in the extremely high threshold level, we missed many true assignments. Conclusion and Prospects: The different identification result of the search engines was mainly caused by the different scoring algorithms. Usually in proteomics high-scored peptides are selected and low-scored peptides are discarded. Many of them are true negatives. By integrating the search results from different parameter and different search engines, the protein identification process can be improved.

• Korean Journal of Culture and Social Issue
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• v.11 no.3
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• pp.67-89
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• 2005

This overview study of mass culture is based on an academic point of view and based thereon an investigation of mass culture from a psychological standpoint follows. This study reviewed previous studies of mass culture that have been done so far divided into Producer-oriented, Text Decoding-oriented, and Recipient Theory, according to subjects of the study. The studies were also reviewed from the viewpoints of industry, consumer science, education, and developmental psychology. Further, it was discussed how trends and limitations in research were covered according to each viewpoint on mass culture. Based on the analysis, this study aims to promote overall psychological interest in studies of mass culture; to present the necessity of analysis and measurement of emotional experience on mass culture; to increase the roles of industrial and consumer science approaches in terms of planning and culture; to change the viewpoint of developmental psychology that accepts youth culture positively; and, to present interdisciplinary studies related to mass culture. Mass culture has already penetrated deeply into real life but there are few analyses and interpretations of mass culture in terms of psychology. This study is meaningful from the aspect that discussion of mass culture has been placed in a position that recognizes the entity of and interest in mass culture. Through this study, I hope that the scope of interest in psychology will expand and that approaches to mass culture will become more diversified.

• Mass Spectrometry Letters
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• v.4 no.3
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• pp.41-46
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• 2013

The goal of this study is to find an experimental condition which enables us to perform enzymatic studies on the cellular behavior of PTEN (phosphatase and tensine homolog) through identification of molecular species of phosphatidylinositol 3,4,5-trisphosphates and their quantitative analysis in a mammalian cell line using mass spectrometry. We initially exployed a two-step extraction process using HCl for extraction of phosphatidylinositol 3,4,5-trisphosphates from two mammalian cell lines and further analyzed the extracted phosphatidylinositol 3,4,5-trisphosphates using tandem mass spectrometry for the identification of them. We finally quantified the concentration of phosphatidylinositol 3,4,5-trisphosphates using internal standard calibration. From these observation, we found that HEK 293-T cells is a good model to examine the enzymatic behavior of PTEN in a cell, and the minimum amount of phosphatidylinositol 3,4,5-trisphosphates is more than 50 pmol for quantification in a mass spectrometer. These results suggest that the well-optimized experimental conditions are required for the investigation of the cellular PTEN in terms of the catalytic mechanism and further for the detailed identification of cellular substrates.

• Tribology and Lubricants
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• v.30 no.4
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• pp.240-245
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• 2014

Low alloy heat resistant brake disk and sintered brake pad are applied to mechanical brake system for the speed-up of urban rapid transit. In this research, we analyzed how the frictional characteristics between brake disk and pad are influenced by the disk mass. At a high disk mass, the friction stability was the lower value as a result of the lack of tribofilm formation at the disk surface. Wear rates of friction materials showed the higher value at a low disk mass and wear rates of 10 mm and 15 mm showed the similar level. Average friction coefficient was the lower value at the 10 mm disk thickness and range of variation of average friction coefficient was also the smaller value at the 10 mm disk thickness. However, there were no significant changes in the friction coefficients under any of test conditions. Surface roughness of a disk showed the highest value at the 5 mm disk and surface roughnesses of 10 mm and 15 mm showed the similar level. As a result, friction characteristics of disk mass influenced the friction stability, as well as the wear rate of friction pad and disk, but not the friction coefficient.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.23 no.11 s.170
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• pp.1940-1951
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• 1999

In this paper, the equations of motion of a Bernoulli-Euler cantilever beam fixed on a moving cart and carrying a lumped mass concentrated at an arbitrary position along the beam is derived. The motion of the beam-mass-cart system is analyzed through unconstrained modal analysis, and a unified characteristic equation for calculating the natural frequencies of the system is obtained. The changes of natural frequencies and the corresponding mode shapes with respect to the changes in mass ratios of the system and to the concentrated position of the lumped mass are investigated with the frequency equation, which can be generally applied to this kind of systems. The exact and assumed-mode solutions including the dynamics of the base cart are obtained, and the open-loop responses of the system by arbitrarily designed forcing function are given by numerical simulations. The results match well with physical phenomena even at the extreme cases where the concentrated mass is attached to the bottom and to the top of the beam.

• Bulletin of the Korean Chemical Society
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• v.26 no.2
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• pp.303-308
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• 2005

A miniaturized time-of-flight mass spectrometer with an electron impact ionization source and sheet membrane introduction has been developed. The advantages and features of this mass spectrometer include high sensitivity, simple structure, low cost, compact volume with field portability, and ease of operation. A mass resolution of 400 at m/z 78 has been obtained with a 25 cm flight path length. Under optimized conditions, the detection limits for the volatile organic compounds (VOCs) studied were 0.2-10 ppm by volume with linear dynamic ranges greater than three orders of magnitude. The response times for various VOCs using a silicone membrane of 127 $\mu$m thickness were in the range 4.5-20 s, which provides a sample analysis time of less than 1 minute. These results indicate that the membrane introduction/time-of-flight mass spectrometer will be useful for a wide range of field applications, particularly for environmental monitoring.

• Anxiety and mood
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• v.8 no.2
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• pp.71-78
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• 2012

The mass social trauma, such as organized violence, wars, oppression by dictatorships and massive terrorist attacks, exposes thousands of people to trauma in a short period of time. Therefore, the mass social trauma is distinguished from individualized trauma, such as a violent attack, rape or a traffic accident in that it results in multiple and extended consequences beyond the individual. During the Khmer Rouge regime, one quarter of the Cambodian population was killed as a result of malnutrition, forced labor and mass killings. Until now, its evil continues to affect Cambodian's physical and mental health problems. Although there is ongoing debate, to date, no consensus has been reached supporting a clear set of recommendations for the intervention and longitudinal study regarding the influence of killing field massacre being too little. And comparative cultural studies, such as comparing the East to West or other Asian cultures are also lacking. This article gives an overview of previous study results about the mental health of Cambodians, and suggests a possible research issue and therapeutic interventions to determine the impact of mass trauma to the members of society and post-traumatic recovery factors.

• YAKHAK HOEJI
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• v.57 no.6
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• pp.412-419
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• 2013

Drug discovery and development processes are time consuming and costly endeavors. It has been reported that on average it takes 10 to 15 years and costs more than $ 1billion to bring a molecule from discovery to market. Compounds fail for various reasons but one of the significant reasons that accounts for failures in clinical trials is poor prediction/understanding of pharmacokinetics and drug metabolism in human. In an effort to improve the number of compounds that exhibit optimal absorption, distribution, metabolism, elimination (ADME), and pharmacokinetic properties in human, drug metabolism, pharmacokinetic scientists have been continually developing new technologies and compound screening strategies. Over the last few years, accelerator mass spectrometry (AMS) and its applications to preclinical/clinical pharmacokinetics and ADME studies have significantly increased, particularly for new chemical/biological entities that are difficult to support with conventional radiolabel studies. In this review, the application of AMS for micro-dosing, micro-tracer absolute bioavailability, mass balance and metabolite profiling studies will be discussed.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.69.2-69.2
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• 2021

We present the discovery of rare active galactic nuclei (AGNs) in nearby (z<0.05) compact elliptical galaxies (cEs) located in isolated environments. Using spectroscopic data from the Sloan Digital Sky Survey (SDSS) Data Release 12, four AGNs were identified based on the optical emission-line diagnostic diagram. SDSS optical spectra of AGNs show the presence of distinct narrow-line emissions. Utilizing the black hole (BH) mass-stellar velocity dispersion scaling relation and the correlation between the narrow L([OIII])/L(Hβ) line ratio and the width of the broad Hα emission line, we estimated the BH masses of the cEs to be in the range of 7×10⁵-8×10⁷ solar mass. The observed surface brightness profiles of the cEs were fitted with a double Sérsic function using the Dark Energy Camera Legacy Survey r-band imaging data. Assuming the inner component as the bulge, the K-band bulge luminosity was also estimated from the corresponding Two Micron All Sky Survey images. We found that our cEs follow the observed BH mass-stellar velocity dispersion and BH mass-bulge luminosity scaling relations, albeit there was a large uncertainty in the derived BH mass of one cE. In view of the observational properties of BHs and those of the stellar populations of cEs, we discuss the proposition that cEs in isolated environments are bona fide low-mass early-type galaxies (i.e., a nature origin).

• Journal of Applied Biological Chemistry
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• v.56 no.1
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• pp.53-57
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• 2013

In here, we investigated the quantitative analysis method of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) with liquid chromatography-mass/mass spectrometry (LC-MS/MS) or gas chromatography-MS. Two ways of clean-up process were investigated for LC-MS/MS instrumental analysis of IAA, but both a simple dilution and hydrophile-lipophile balance (HLB) solid phase extraction (SPE) were not met the optimal recovery rates for quantitative analysis. On the other hand, the clean-up method for GC-MS was finally optimized through HLB-SPE from 250-folds diluted sample and methylation with trimethylsilyl chloride in methanol for 4 h. The limit of detection for methyl ester of IAA and IBA were both 1.4 mg/L, and recovery rates showed 93-107% from the concentrated liquid fertilizer.