• Molecules and Cells
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• 제27권5호
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• pp.601-608
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• 2009

The 2-deoxystreptamine and paromamine are two key intermediates in kanamycin biosynthesis. In the present study, pSK-2 and pSK-7 recombinant plasmids were constructed with two combinations of genes: kanABK, and kanABKF and kacA respectively from kanamycin producer Streptomyces kanamyceticus ATCC12853. These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans SK-2/SL and S. lividans SK-7/SL, respectively. ESI/ MS and ESI-LC/MS analysis of the metabolite from S. lividans SK-2/SL showed that the compound had a molecular mass of 163 $[M+H]^+$, which corresponds to that of 2-deoxystreptamine. ESI/MS and MS/MS analysis of metabolites from S. lividans SK-7/SL demonstrated the production of paromamine with a molecular mass of $324[M+H]^+$. In this study, we report the production of paromamine in a heterologous host for the first time. This study will evoke to explore complete biosynthetic pathways of kanamycin and related aminoglycoside antibiotics.

• 한국인터넷방송통신학회논문지
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• 제8권6호
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• pp.35-40
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• 2008

최근 사파이어기판 위에 성장한 갈륨나이트라이드 발광다이오드의 소자 제작 기술이 비약적으로 발전하였다. 하지만 이들 다이오드가 이미 상업적으로 활용되고 있다 할지라도 갈륨나이트라이드 발광다이오드에 있어서 다이오드를 구성하는 물질들과 소형화에 따른 정전기 방전에 의한 피해를 고려해야한다. 정전기방전(ESD)에 의한 피해는 발광소자의 신뢰성에 매우 큰 영향을 주는 파라미터중 하나이다. 본 연구에서는 대량생산 되는 발광다이오드의 생산공정에서 발생하는 정전기방전에 의한 피해와 이에 대한 대책을 논의하였다. 대부분의 ESD 문제는 장비의 적정한 사용과 공정 환경 개선을 통해서 제어되었다.

• 패션비즈니스
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• 제7권2호
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• pp.26-36
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• 2003

The purpose of this study is to examine the concept of modern consumer society and to analyze how the meaning of postmodern-feminism is consumed into what image of preference in fashion. The function of modern society has changed into the system which is centered around consumption from the one that emphasizes labor and production of mass consumption age by mass production. In this consumer society, consumption means just not to waste of production but to consume the commodity sign reflected on the desire of a moderner. In other words, it means what is consumed will be the meaning, preference, symbol, and image mood not goods itself with physical feature. Existing feminism has affected by postmodernism. Due to that, postmodern-feminism has developed, taking to pieces the paternal argument since the late 1960s. It has tried to give up hope this idea, regarding sex distinction as a socially organized category contrary to women's identity of biological aspect suppressed in the paternal system of value. Especially it has demanded only one type on sex should be translated into a distinctive quality, multiple meaning, and sex. Accordingly in modern fashion, this aspect is expressed like the followings : distinction into women's image evaded fixation, multiple meaning into image of androgyny, multiple sex into that of mixture. And this is used as the image of symbolic goods, also the purpose of brand difference.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 추계학술대회
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots ($15^{10}$ /year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above $50\%$) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• 지반과기술
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• 제3권3호
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• pp.15-22
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• 2006

The optimum blasting pattern to excavate a quarry efficiently and economically can be determined based on the minimum production cost, which is generally estimated according to rock fragmentation. Therefore, it is a critical problem to predict fragment size distribution of blasted rocks over an entire quarry. By comparing various prediction models, it can be ascertained that the result obtained from Kuz-Ram model relatively coincides with that of field measurements. Kuz-Ram model uses the concept of rock factor to signify conditions of rock mass such as block size, rock jointing, strength and others. For the evaluation of total production cost, it is imperative to estimate 3-D spatial distribution of rock factor for the entire quarry. In this study, a sequential indicator simulation technique is adopted for estimation of spatial distribution of rock factor due to its higher reproducibility of spatial variability and distribution models than Kriging methods. Further, this can reduce the uncertainty of predictor using distribution information of sample data. The entire quarry is classified into three types of rock mass and optimum blasting pattern is proposed for each type based on 3-D spatial distribution of rock factor. In addition, plane maps of rock factor distribution for each ground level are provided to estimate production costs for each process and to make a plan for an optimum blasting pattern.

• 신재생에너지
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• 제7권1호
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• pp.29-35
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• 2011

The waste treatment fee and energy production effect of Wonju city RDF plant, the first RDF manufacturing plant in Korea, were investigated in the study. All plant operation data, like total weight of received wastes, produced RDF and separated rejects in processes were fully recorded for mass balance calculation of the plant in 2009. Also all consumed oil and electricity were recorded for energy balance calculation. The results showed that the waste treatment fee not including the RDF sales price of 25,000 won/ton-RDF was 116,573 won/ton-MSW and it went down to 105,298 won when included the RDF price. Produced RDF was 40.2% of total received waste in weight. Three components analysis by mass balance calculation of total received waste showed that Wonju city's MSW was 32.4% of combustible, 37.5% of water and 30.1% of incombustible respectively. Energy effect was found that total amount of produced energy was about 4 times more than that of consumed energy.

• 소성∙가공
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• 제18권8호
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• pp.633-639
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• 2009

Remarkable progress had been made in both technology and production of optical elements including aspheric lens. In the past, precision optical glass lenses were produced through multiple processes such as grinding and polishing, but mass production of aspheric lenses requiring high accuracy and having complex profile was rather difficult. Against such a background, the high-precision optical GMP process was developed with an eye on mass production of precision optical glass pasts by molding press. This GMP process can produce with precision and good repeatability special form lenses such as for cameras, video cameras, aspheric lenses for optical items. In this study, Design Of Experiment(Taguchi method) was adopted to find a tendency of molding conditions that influence formability. Three main factors for molding conditions were selected based on pressure at pressing stage and temperature, pressure at cooling stage. Also, the DOE was carried out and the interference patterns were measured to evaluate the formability of GMP process. From the results, it was found that the cooling pressure is the most sensitive parameter for progressive GMP process.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2008년도 International Symposium on Remote Sensing
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• pp.375-377
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• 2008

The KOMPSAT2 Terminal Polar Station was recently installed at near North Pole, Tromso, and Toulouse. The K2PS consists of one receiving station and two processing sites. The receiving station has been installed at SvalSat ($N78^{\circ}$, $E15^{\circ}$ ), and the two receiving sites have been installed at KSAT (Kongsberg Satellite Service AS), Tromso, Norway ($N69^{\circ}$ ,$E18^{\circ}$ ) and SISA, Toulouse, France ($N43^{\circ}$ ,$E1^{\circ}$ ). The products ofK2PS system can be classified to two categories: Level 1R product and Level 1G product. The Level 1R product is radiometric corrected product with RPC (Rational Polynomial Coefficients) and the Level 1G product is geometric corrected product with POD (Precise Orbit Data) and PAD (Precise Attitude Data) data based on Level 1R product. To meet a SISA (Spot Image SA)'s requirement, K2PS system has high performance product producing capability. This paper describes overall K2PS systems' production generation flow and the mass production test result of K2PS systems.

• 한국실내디자인학회논문집
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• 제6호
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• pp.53-62
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• 1995

This research aims at proving the fact that the forms, spaces and many other design concepts of Modernism are much related with the changes of materials, struc-tures, and the way of construction as well as the idealis-tic and aesthestic things through the history of steel, one of the most important materials of the style. The meaning steel has in the modern architecture can be studied in the structure and industrial production system. 1) Steel frame broadened the range of understanding the space and created the new form through the skeleton/skin structure by reinterpreting the existing space fac-tors while it was being adopted to the architecture. Walls could be freed from the traditional function of bear-ing wall and roofs gave the transparancy to the interior by being linked with the glass. Posts lost the function which confines the space in the frame of the grid system and gave the flexibility to the interior due to the economical materials. These changes made the movable partition, screen with various materials and the system furniture which divides the space more important. 2) In the aspect of the industrialized architecture, it be-came the moment that the most of the archtectural com-posing parts were in mass production as they were standarized, high qualified, and generalized by the indus-trial characteristics of steel, and the specialization of structure and cladding, but the neither of the efforts to make the building itself by mass production or to standarize it was fulfilled. The high-tech architecture which borrows its archtectural manifestation from the high technology, however, is consistently paying efforts on such industrialization.

• 한국실내디자인학회논문집
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• 제22권5호
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• pp.106-113
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• 2013

Since the 1960's, diverse movements that oppose the uniform design tendency of modernism appears in Italy. Especially, Gaetano Pesce, who opposed uniformity and standardization that are based on mass production system, had interests in making unique product, not an identical product, while accepting modern production technology. Therefore, the purpose of this study is to comprehend Pesce's design, which creates diversity by arranging contingent, non-predictive, and episodic aspects in creative and acceptance processes according to the intension of the designer within mass production system, as a non-representational characteristic, which appears widely in modern thought and the arts, and examine how it is materialized in detailed design work. The study methods are first examining non-representation with theoretical contemplation and dealing with the design philosophy of Pesce based on the examination. Next, in order to discover non-representational characteristics that appear in the work of Pesce, it examines from introducing worker's creativity, user's possibility of choice, openness in the working process, and the aspects of researching materials. And a specific application method will be drawn through case analysis centered on furniture design. Gaetano Pesce's design that denies the world of representation based on the sameness comes to possess non-representational characteristics that acknowledge contingency and ceaselessly create difference by intentionally arranging during the working process.