• Molecules and Cells
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• v.21 no.3
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• pp.411-417
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• 2006

We have developed a polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) marker that can distinguish male-fertile (N) and male-sterile (S) cytoplasm in onions. The PCR-RFLP marker was located in a chloroplast psbA gene amplicon. Digesting the amplicons from different cytoplasm-containing varieties with the restriction enzyme MspI revealed that N-cytoplasm plants have a functional MspI site (CCGG), whereas the S-cytoplasm plants has a substitution in that site (CTGG), and thus no MspI target. The results obtained using this PCR-RFLP marker to distinguish between cytoplasmic male sterile factors in 35 onion varieties corresponded with those using a CMS-specific sequence-characterized amplified region (SCAR) marker. Moreover, the PCR-RFLP marker can identify N- ot S-cytoplasms in DNA sample mixtures in which they are in up to a 10-fold minority, indicating that use of the marker has high diagnostic precision. We also demonstrated the usefulness of the SNP detected in the psbA gene for high-throughput discrimination of CMS factors using Real-time PCR and a TaqMan probe assay.

• Speech Sciences
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• v.8 no.4
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• pp.289-305
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• 2001

The conjunctive marker '-ko' in Korean can be interpreted as meaning either conjunctive 'and' or ordering 'and then'. The interpretation of '-ko' is ambiguous in written texts but not in spoken texts. It is because the meaning of the utterance is determined by the combination of the text with its prosody. The two meanings of ' -ko' can be explained by the theory of implicature, which was introduced by Grice (1973, 1981). This paper examines the meaning of the marker '-ko' with respect to the relation between its meaning and prosody. The results of the experiments in this paper showed that the prosodic phrasing in Korean influences the interpretation of the marker '-ko'. When two constituents combined by '-ko' are realized in the same accentual phrase, the marker can be interpreted as meaning 'exactly be orderly'. This meaning can be classified as the Particularlized Conversational Implicature (PCl) in Gricean theory. In the other cases of phrasing, the marker '-ko' can mean either 'conjunctive' or 'be orderly' by the Generalized Conversational Implicature (GCI). The fact that phrasing determines the interpretations of the marker '-ko' can be seen as supporting the view that prosody interacts with various levels of linguistic phenomena from phonology to pragmatics.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.7B
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• pp.711-721
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• 2004

To provide the end-to-end service differentiation for assured services, the random early demotion and promotion (REDP) marker in the edge router at each domain boundary monitors the aggregate flow of the incoming in-profile packets and demotes in-profile packets or promotes the previously demoted in-profile packets at the aggregate flow level according to the negotiated interdomain service level agreement (SLA). The REDP marker achieves UDP fairness in demoting and promoting packets through random and early marking decisions on packets. But, TCP fairness of the REDP marker is not obvious as fur UDP sources. In this paper, to improve TCP fairness of the REDP marker, we combine a dropper with the REDP marker. To make packet transmission rates of TCP flows more fair, at the aggregate flow level the combined dropper drops incoming excessive in-profile packets randomly with a constant probability when the token level in the leaky bucket stays In demotion region without incoming demoted in-profile packets. It performs a dropping in the demotion at a domain boundary only if there is no prior demotion. The concatenate dropping at multiple domains is avoided to manifest the effect of a dropping at a domain boundary on TCP fairness. We experiment with the REDP marker with the combined dropper using ns2 simulator for TCP sources. The simulation results show that the REDP marker with the combined dropper improves TCP fairness in demoting and promoting packets by generating fair demoted in-profile traffic compared to the REDP marker. The effectiveness of the selected drop probability is also investigated with showing its impact on the performance of the REDP marker with the combined dropper.

• Journal of the Korea Society of Computer and Information
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• v.13 no.1
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• pp.89-97
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• 2008

In this paper, we present a method for the enhancement of marker detection correctness and marker recognition speed by using artificial neural network. Contours of objects are extracted from the input image. They are approximated to a list of line segments. Quadrangles are found with the geometrical features of the approximated line segments. They are normalized into exact squares by using the warping technique and scale transformation. Feature vectors are extracted from the square image by using principal component analysis. Artincial neural network is used to checks if the square image is a marker image or a non-marker image. After that, the type of marker is recognized by using an artificial neural network. Experimental results show that the proposed method enhances the correctness of the marker detection and recognition.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.134-141
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• 2016

The DNA marker T1ex, originally developed from melon (Cucumis melo L.) for monoecy, was employed in chamoe, which is referred to as oriental melon. This marker shows size variations in monoecious melon. However, in chamoe, no such detrimental size variation was found in monoecious chamoe, and 99% association between flower phenotypes and genotypes of the T1ex marker was observed in 106 lines of chamoe. To evaluate the efficacy of the T1ex marker for marker-assisted selection (MAS), a total of 240 plants of chamoe breeding lines were screened using the T1ex marker. Among these, 98 varieties were selected. Although the T1ex marker might not be useful for MAS in melon, we found 100% concordance between genotypes and phenotypes for sex expression in chamoe. These results suggest that the T1ex marker will be a useful resource for MAS for monoecy in chamoe.

• Journal of Convergence for Information Technology
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• v.12 no.1
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• pp.1-6
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• 2022

As augmented reality technology becomes more common and prevelant, marker-based AR contents are applied in various ways. However AR contents are still hardly utilized due to the low recognition rate of marker images. In order to increase the recognition rate of AR marker images, this paper experiment and analyzed how much the recognition rate of markers could be improved when image correction and design changes was applied. The experimental result shows that the image correction task could significantly improve the number of image characteristics and the recognition grade if the image was modified in a way its saturation value is increased. Moreover, the recognition rate was improved even more when regular pattern design was added to the original marker image. In conclusion, it was possible to make the marker well recognized through proper correction of the image and additional process of pattern design in the process of producing the marker image.

• Journal of the Korea Institute of Building Construction
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• v.23 no.5
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• pp.629-638
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• 2023

This study introduces an innovative approach to monitor the whereabouts of workers within indoor construction settings. While traditional modalities such as GPS and NTRIP have demonstrated efficacy for outdoor localizations, their precision dwindles in indoor environments. In response, this research advocates for the adoption of Aruco markers. Leveraging computer vision technology, these markers facilitate the quantification of the distance between a worker and the marker, subsequently pinpointing the worker's instantaneous location with heightened accuracy. The methodology's efficacy was rigorously evaluated in a real-world construction scenario. Parameters including system stability, the influence of lighting conditions, the extremity of measurable distances, and the breadth of recognition angles were methodically appraised. System stability was ascertained by maneuvering the camera at a uniform velocity, gauging its marker recognition prowess. The impact of varying luminosity on marker discernibility was scrutinized by modulating the ambient lighting. Furthermore, the camera's spatial movement ascertained both the upper threshold of distance until marker recognition waned and the maximal angle at which markers remained discernible.

• Molecules and Cells
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• v.20 no.1
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• pp.30-34
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• 2005

This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.34-34
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• 2003

한국산 선발계통 및 일본산 양식계통과 이들 두 계통간 잡종 참돔 집단의 유전적 특성을 분석하기 위하여, RAPD (Random Amplified Polymorphic DNA) marker를 탐색하였다. 10개의 염기로 이루어진 200개의 random primer 분석을 통하여 polymorphic pattern을 나타내는 23개의 random primer를 선발하였으며, 각 primer의 재현성을 확인하였다. 이들 중 OPA-11 primer는 크기가 각각 600 bp, 650 bp 및 750 bp 인 3개의 DNA 단편에 의하여 4개의 genotype을 나타냈으며, 각 genotype의 빈도는 집단간차이를 보였고, 한국산 선발계통 집단에서는 4개의 genotype이 모두 발견되는 반면, 일본산 양식계통 및 일본산 양식계통을 포함한 교배집단에서는 특정 genotype만 발견되었다. OPA-11 primer 유래의 polymorphic DNA 단편을 cloning하고 염기서열을 결정하였으며, SCAR (Sequence Characterized Amplified Region) primer를 제작하고 분석하였다. 본 연구는 참돔집단의 유전적 특성 파악 및 집단 구별에 RAPD marker를 활용하였으며, 참돔 육종시 형질 및 기능관련 DNA marker 탐색에 적용하기 위하여, 이후의 연구에서는 SCAR과 RFLP 분석에 RAPD marker를 이용하여 100% 정확도를 갖는 RFLP maker를 찾고, MAS (Marker-Assisted Selection)에 적용하고자 한다.

• Speech Sciences
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• v.6
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• pp.55-82
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• 1999

The purpose of this paper is to investigate the acoustic characteristics of the Korean accusative marker {-lil} which functions as a discourse marker in discourse. Generally, in written texts or read speeches, it is seldom omitted and it certainly seems to serve a grammatical function. But in ordinary discourse, speakers do not use it in many cases. That is, the environments speakers use {-lil} differ from those they do not. According to the semantic interpretations, {-lil} functions as a pragmatic factor and adds to the meaning of the object in an utterance. In this paper, by comparing the acoustic characteristics of the utterances that contain the marker {-lil} with those of utterances that do not, especially based on Korean Intonational Phonology, we will demonstrate that the Korean accusative marker {-lil} shows clearly the acoustic characteristics related to the pragmatic factors which reflect speakers' special intention.