• Ocean and Polar Research
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• v.45 no.3
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• pp.113-123
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• 2023

Humic substances are refractory organic compounds that are relatively low in biological activity but known to stimulate phytoplankton growth in estuarine and marine environments. The effect of humic substances on phytoplankton growth was investigated in the seawater zone of the Yeongsan River estuary where an episodic anthropogenic freshwater is discharged, affecting its water properties directly. Water samples and data of water properties were collected at three stations (Sts. A-C) along the channel of the seawater zone in February, May, August and November, 2009. The collected water samples were incubated after humic acids (HA) were added in mesocosm experiments. Phytoplankton (chlorophyll-a) were fractionated into net- (> 20 ㎛) and nano-size (< 20 ㎛) to examine the response of phytoplankton according to size. Their response to HA treatment was assessed by repeated measures analysis of variance (RM-ANOVA). The experiments showed that phytoplankton biomass (chlorophyll-a) significantly increased after HA were added at the stations near the sea dike. Especially, nano-sized chlorophyll-a concentrations increased significantly throughout the seasons. This indicates that understanding the behavior of refractory organic matters such as humic substances is required to better manage altered estuarine ecosystems including the Yeongsan River estuary which are affected by episodic discharge of freshwater from sea dikes.

• Ocean and Polar Research
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• v.25 no.2
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• pp.213-226
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• 2003

Kongsfjorden near Korean Arctic Station, Dasan, is a glacial fjord in the Svalbard archipelago, Arctic that is influenced by both Atlantic and Arctic water masses. During the Arctic field season August 2002, surface temperature, salinity, density, and phytoplankton biomass (chi a) was measured in Kongsfjorden. A total of 15 surface samples were collected for the phytoplankton related measurements. Chl a values ranged from 0.08 to 1.4mg chi a $m^{-3}$ (mean of 0.53mg chl a $m^{-3}$) in the overall surface stations. The highest values of the chi a concentrations (> 1.0mg chi a $m^{-3}$) were found near glacier in the northeastern part of Kongsfjorden. Nanoplanktonic (< $20{\mu}m$) phytoflagellates were important contributors for the increase of the chi a. The nano-sized phytoflagellates accounted for more than 90% of the total chi a biomass in the study area. Surface temperatures and salinities ranged from 2.5 to $7.18^{\circ}C$ (mean of $4.65^{\circ}C$) and from 22.55 to 32.97 psu (mean of 30.16 psu), respectively. The physical factors were not highly correlated with phytoplankton distribution. The character of surface water due to down-fjord wind was highly similar to phytoplankton distribution. Drifting ice, freshwater, and semdiment inputs from large tidal glaciers located in the inner part of Konsfjorden create steep physico- and biogeochemical environmental gradients along the length of this ford. The glacial inputs cause reduced biodiversity biomass and productivity in the pelagic community in the inner fjord. Primary production of benthic and pelagic microalgae is reduced due to the limited light levels in the turbid and mixed inner waters. The magnitude of glacial effects diminishes towards the outer fjord. Kongsfjorden is an important feeding ground fer marine mammals and seabirds. Especially, seabirds play the largest energy intake and also export nutrients for primary production of the marine microalgae. Kongsfjorden has received a lot of research attention as a site for exploring the impacts of climate changes. Dasan Station in Kongsfjorden will be an important Arctic site for monitoring and detecting future environmental changes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7761-7772
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• 2015

This study describes the spatio-temporal distributions in phytoplankton community such as species composition, standing crops and dominant species from April to November 2008 in the Jeju Marine Ranching Area (JMRA). A total of 106 species of phytoplankton belonging to 63 genera was identified. In particular, diatoms and dinoflagellates were occupied more than 61% and 35% of total species, respectively. The annual dominant species were Skeletonema costatum-like species(ls) in April, Torodinium teredo, Cylindrotheca closterium, Scrippsiella trochoidea in June, S. costatum - ls, Thalassionema nitzschioides, Pseudo-nitzschia pungens, Ebria tripartita in September and Corethron pennatum, Dictyocha fibura, Neoceratium teres in November. Phytoplankton cell density ranged between $0.6cells{\cdot}mL^{-1}$ in June and $64.0cells{\cdot}mL^{-1}$ in April. It fluctuated with an annual mean of $11.7cells{\cdot}mL^{-1}$ between the lowest value of $0.9cells{\cdot}mL^{-1}$ in November and the highest value of $37.0cells{\cdot}mL^{-1}$ by S. costatum -ls in April. Diversity index in September was higher than diatom blooming seasons in April. According to the phytoplankton community structure, the biological oceanographic characteristics of the JMRA was characterized by nanoplankton during a year. That is, primary production is deemed to have a higher possibility of being adjusted by a reproduction by material cycle in the ecosystems than nutrients supply from the lands.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.4
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• pp.223-231
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• 2014

In order to prevent the spread of non-indigenous aquatic species through the ballast water in commercial ships, International Maritime Organization (IMO) adopted in 2004 the International Convention for Control and Management of Ship's Ballast Water and Sediments. The Convention mandates treatment of ballast water for most transoceanic voyages and its confirmation of treatment is made with plankton live/dead assay. Fluorescein diacetate assay (FDA), which produces bright green light for live phytoplankton, has been a de facto standard method to determine the survival of marine plankton, but its staining efficacy has been in dispute. In the present study, we examined the limitation of FDA, and compared its efficacy with Neutral red (NR) staining, another promising assay and widely used especially for zooplankton mortality. For all phytoplankton species studied in the present study, except Ditylum brightwellii, the staining efficiency was <50% with FDA. The green FDA fluorescence interfered with phytoplankton autofluorescence in most samples. In contrast, NR assay stained over 90% of both phytoplankton and zooplankton species tested in this study. FDA assay also showed that green FDA fluorescence rapidly faded when phytoplankton cells were exposed to microscope light. Both FDA and NR assay were negative on formalin-killed individuals of both phytoplankton and zooplankton species. Our results suggest that NR assay is more effective for determining the survival of marine plankton and can be applied to test the efficacy of ballast water treatment.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.5
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• pp.474-483
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• 2015

In order to understand the seasonal change of phytoplankton as well as the effect of water physico-chemical parameters, we investigated 18 stations in coastal areas of the East Sea in February, May, August and November in 2009. The taxa of phytoplankton observed in this study were classified as 37 Bacillariophyceae, 22 Dinophyceae, 1 Euglenophyceae, 3 Dictyophyceae and 1 Cryptophyceae. Phytoplankton abundance ranged from $1.2{\times}10^3cells/L$ to $246.6{\times}10^3cells/L$(with a mean value of $24.8{\times}10^3cells/L$), the highest biomass was observed in May. The dominant species were Leptocylindrus danicus, Chaetoceros affinis, Pseudo-nitzschia pungens, Thalassionema nitzschioides and etc. Pearson's correlation co-efficient between phytoplankton abundance and other water parameters showed the positive relationships with pH, DO, Secchi-disk depth, and SS, the negative relationships with $SiO_2-Si$. Seasonal patterns of phytoplankton dominant species were affected by the characteristics of water masses based on T-S diagram analysis. In particular, phytoplankton distributional patterns were related with water temperature in May and salinity in August, respectively. According to the result of MDS(Multi-dimensional scaling) using the phytoplankton abundance and species composition, the spatial distribution of phytoplankton were characterized with Ganwon(Group A) and Gyeongbuk(Group B) at the coastal areas of Jukbyeon or Uljin.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.5
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• pp.435-444
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• 2023

Suitable environmental conditions in Saemangeum frequently favor phytoplankton growth. There have been occurrences of sudden phytoplankton blooms, surpassing the algae management standards. A model was designed to prevent such blooms using scientific predictive techniques to forecast and regulate the possibility of phytoplankton blooms. We propose effective and efficient algae control measures concerning every phytoplankton species optimized through the policy control of nutrients (DIN, PO4-P) from rivers and controlling lake salinity using gate operations. The probability of phytoplankton blooms was initially forecast using an artificial neural network algorithm based on observations. The model's Kappa number fluctuated from 0.7889 to 1.0000, indicating good to excellent predictive power. The Garson algorithm was then utilized to assess the significance of explanatory variables for every species. Meanwhile, the probability of phytoplankton blooms was anticipated depending on the DIN and salinity value changes. Therefore, the model predicted the precise DIN and salinity concentrations to inhibit phytoplankton blooms for each species. Hence, the green algae model can create effective proactive measures to avoid future phytoplankton blooms in enormous artificial lakes.

• Korean Journal of Ecology and Environment
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• v.45 no.2
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• pp.139-149
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• 2012

Four in situ incubation experiments were conducted in May, August and November 2009, as well as February 2010 to determine the seasonal primary productivity and the community structure of phytoplankton at the Seomjin estuary. The primary production of phytoplankton ranged from 9 to 3560 mgC $m^{-2}\;d^{-1}$. Primary productivity was the highest in the summer season (August), which was influenced by improved optical and temperature conditions of the water, as well as the supply of nutrients derived from its surrounding watershed. Particularly, the upper station (SJ-1, SJ-2) of Seom-jin estuary showed a higher productivity, as a result of inflow of input nutrients originated from the terrestrial source. The fucoxanthin, as an index pigment of diatoms showed the highest concentration (0.74~9.51 ${\mu}g\;L^{-1}$) at all stations, occupying 30~80% to total Chl a concentrations. The phytoplankton species composition determination, using a microscope showed similar results to the pigment analysis, which indicated diatom Skeletonema costatum, as the dominant species. The primary productivity in Seom-jin estuary indicates temporally and spatially large variation, according to different environmental conditions. Also, Skeletonema costatum has euryhaline features with relatively higher contribution.

• Korean Journal of Environmental Biology
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• v.34 no.4
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• pp.292-303
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• 2016

This study described the spatial distributions of marine environmental factors such as water temperature, salinity, chlorophyll a concentration and turbidity, and characteristics of phytoplankton community such as species composition, standing crops and dominant species at 19 fishing ports around Jeju Island during the early summer of 2016. I analyzed bio-oceanographical characteristics using principal component analysis (PCA) of the environmental factors and biological parameters. Water temperature, salinity, chlorophyll a and turbidity ranged from 17.6 to $20.7^{\circ}C$, from 26.19 to 32.33 psu, from 0.76 to $7.13{\mu}g\;L^{-1}$, and from 0.51 to 14.49 FTU, respectively. A total of 51 species of phytoplankton belonging to 35 genera were identified. In particular, diatoms and dinoflagellates accounted for more than 56.8% and 27.4% of all the species, respectively. Moreover, the number of phytoplankton species was controlled by salinity. Phytoplankton cell density ranged from $2.9cells\;mL^{-1}$ to $185.9cells\;mL^{-1}$. The dominant species were Navicula spp. Stephanopyxis turris, Eutreptiella gymnastica and Mesodinium rubrum. Environmental factors and the phytoplankton community varied greatly between sampling sites. According to PCA, the biological oceanographic characteristics of the around Jeju Island were characterized by meteorological factors such as air temperature, precipitation and discharge of ground water during early summer.

• Korean Journal of Environmental Biology
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• v.38 no.2
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• pp.254-270
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• 2020

We conducted a seasonal field survey to analyze the distribution patterns of a phytoplankton community and biological oceanographic characteristics in the Geum river estuary in 2018. The results showed that the phytoplankton community consisted of 58 genera and 116 species, showing a relatively simple distribution. It was controlled by diatoms at 70.2%, a low number of species in winter and spring, and a high number in summer and autumn. The phytoplankton cell density ranged from 10.0 to 2,904.0 cells mL^-1, with an average layer of 577.2 cells mL^-1, which was low in autumn and high in winter. The seasonal succession of phytoplankton dominant species was mainly centric diatoms from winter to summer, including Thalassiosira nordenskioeldii, Cerataulina bergonii, and Skeletonema costatum-ls in winter, S. costatum-ls and C. bergonii in spring, and Eucampia zodiacus and Th. nordenskioeldii in summer. However, the autumn species depended upon the regions, with the inner bay dominated by the centric diatom, Aulacoseira cf. granulata, the mixed areas by S. costatum-ls, and the open sea by the dinoflagellate, Lingulodinium polyedra. According to principal component analysis (PCA), the phytoplankton community was greatly affected by the inflow and expansion of freshwater, including high nutrients, which are introduced annually through the rivermouth weir in Geum river estuary. However, the estuary, which is strongly affected by annual freshwater, was limited to areas near Geumran Island, which is adjacent to the river-mouth weir.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.3
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• pp.151-164
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• 2010

Field observations on the seasonal variations of environmental factors and phytoplankton community were carried out four times at 30 stations in the narrow strait between Yeosu and Dolsan Island of the Korean South Sea from September 2005 to May 2006. The ranges of water temperature, salinity and extinction coefficient in the surface waters were 5.6~26.3, 25.36~33.92 psu and 0.13~2.13, respectively. The water temperature measured higher at Gamak Bay in summer and spring. It measured higher at Yeosuhae Bay in autumn and winter. Salinity showed uniformity of distributions in almost all areas, except for an area near a sewage disposal outlet. Extinction coefficient indicated that the turbidity of Gamak Bay and the area near the sewage disposal outlet were higher than that of the Yeosuhae Bay. In the phytoplankton community were identified a total of 99 species belonging to 51 genera. The species composition showed itself to be various in summer and autumn, but poor in winter and spring with a high ratio of centric diatoms all the year round. Seasonal succession of dominant species were Skeletonema costatum and Chaetoceros curvisetus in summer, Eucampia zodiacus in autumn and winter, and Chaetoceros affinis and Thalassionema nitzschioides inspring. Standing crops of phytoplankton and Chlorophyll $\alpha$ concentration were greatly higher at Gamak Bay in summer with ranges of $0.2{\times}10^4\;cells\;L^{-1}$ to $296{\times}10^4\;L^{-1}$, and $1.94\;L^{-1}$ to $22.12\;L^{-1}$, respectively. From the results of principal component analysis (PCA), the northern part of Dolsan Island was divided into two or three regions from the characteristics of marine environment and phytoplankton community.