• ALGAE
• /
• v.19 no.2
• /
• pp.145-148
• /
• 2004

As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.

• ALGAE
• /
• v.20 no.2
• /
• pp.113-120
• /
• 2005

The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method ($R^2$ = 0.906), and the blue-green algal cell number determined by microscopic observation ($R^2$ = 0.588), which gives insight into applications to early detection of blue green algal bloom.

• Journal of the korean society of oceanography
• /
• v.32 no.1
• /
• pp.28-37
• /
• 1997

Two marine bivalve shells were collected from the eastern and western coastal regions of Korea, respectively. Stable oxygen and carbon isotope profiles are constructed using the incremental sampling along the axis of maximum growth to provide the continuous ${\delta}^{18}$O and ${\delta}^{13}$C records, which register the physical, biological and chemical properties of seawater where the organisms live. Cycles in the ${\delta}^{18}$O profiles are interpreted as annual along with the identification of annual growth bands; the maximum ${\delta}^{18}$O values correspond with the coldest temperature of seawater whereas the minimum ${\delta}^{18}$O values with the warmest temperature. The primary control on the amplitude of the ${\delta}^{18}$O profiles is seasonal variation of seawater temperature. The offset of the baseline between ${\delta}^{18}$O values of the two specimens is attributed to differences in both temperature and seawater ${\delta}^{18}$O values between two localities. The ${\delta}^{13}$C profiles show the similar seasonality of carbon cycling associated with phytoplankton productivity. The offset in the ${\delta}^{13}$C profiles between two specimens may be, as in the case of oxygen isotope profile, attributed to the different ${\delta}^{13}$C value of the seawater DIC (dissolved inorganic carbon) between the western coast and the eastern coast. Relationships between the shell isotopic composition and the coastal water properties of shell growth are readily interpreted from the ${\delta}^{18}$O-${\delta}^{13}$C pair diagram of the shell isotope data, similar to the use of salinity-${\delta}^{18}$O diagram for identifying water masses. The preliminary stable isotope results of this study suggest that mollusk shell isotope geochemistry may be useful to monitor the properties of water masses in the coastal and inner shelf setting around Korea and improve the interpretation of paleoceanography, provided the fossil mollusks are well preserved.

• Ocean and Polar Research
• /
• v.35 no.2
• /
• pp.147-155
• /
• 2013

The purpose of this study is to investigate seasonal difference in linear trends in satellite-derived chlorophyll-a concentration (Chl-a) and their related environmental changes in the South Sea of Korea (SSK) and East China Sea (ECS) for recent 15 years (Jan. 1998~Dec. 2012) by analyzing climatological data of Chl-a, Rrs(555), sea surface wind (SSW) and nutrient. A linear trend analysis of Chl-a data reveals that, during recent 15 years, the spring bloom was enhanced in most of the ECS, while summer and fall blooms were weakened. The increased spring (Mar. - May) Chl-a was associated with strengthened winter (Dec. - Feb.) wind that probably provided more nutrient into the upper ocean from the deep. The causes of decreased summer (Jun. - Aug.) Chl-a in the northern ECS were uncertain, but seemed to be related with the nutrient limitation. Recently (after 2006), low-salinity Changjiang diluted water in the south of Jeju and the SSK had lower phosphate that caused increase in N/P ratio with Chl-a decrease. The decreased fall (Sep. - Nov.) Chl-a was associated with weakened wind that tends to entrain less nutrient into the upper ocean from the deep. This study suggests that phytoplankton in the ECS differently changes in response to environmental changes depending on season and region.

• Proceedings of KOSOMES biannual meeting
• /
• 2009.06a
• /
• pp.149-150
• /
• 2009

영산강 하구둑에 의해 폐쇄내만의 조건을 가진 영산강 하구역에서 식물플랑크톤의 계절변동을 파악하고자 2008년에 계절별로 7개 정점에서 조사하였다. 영산강 하구역에서 출현한 식물플랑크톤 개체수는 14$\sim$34,958cells/mL의 범위를 보였다. 조사시기별로는 7월에 평균 10,796cells/mL가 출현하여 조사기간 중 가장 많은 출현 개체수를 보였고, 이어서 9월(평균 3,327cells/mL), 5월(평균 590cells/mL), 11월(평균 34cells/mL) 순으로 감소하여 조사시기별 출현개체수의 변동이 매우 심하였다. 규조류는 모든 조사시기에 걸쳐 최고의 점유율을 나타내었고 11월을 제외하면 전체 개체수의 90%이상을 차지하고 있어 규조류가 영산강 하구역의 식물플랑크톤 생물량을 좌우하고 있는 종임을 알 수 있었다. 영산강 하구역에서 출현한 식물플랑크톤의 제 1 우점종은 5월에 Eucampia zodiacus(83.1%), 7월에 Chaetoceros curvisetus(24.2%), 9월에 Pseudo-nitzschia delicatissima(94.3%), 11월에는 Chroomnnas류(33.6%)로 변화하였다. 5월과 9월은 한 종이 전체 식물플랑크톤 개체수의 80% 이상을 차지하는 극우점양상을 보여주었고, 7월에는 C. curvisetus, Skeleton듬 costatum 및 Chaetoceros sp. 등이 유사한 점유율로 우점하고 있었다. 영산강 하구역의 식물플랑크톤 종다양성지수는 0.228$\sim$2.260의 범위로 소수의 우점종에 의해 전체 군집의 분포양상이 결정되는 전형적인 연안, 하구수역의 군집 특성을 보이고 있었다.

• Journal of the korean society of oceanography
• /
• v.35 no.1
• /
• pp.56-64
• /
• 2000

The importance of terrestrial organic matter as a food source for a brackish water clam Corbicula japonica was evaluated using stable carbon isotope ratios (${\delta}^{13}$C) in its tissues and potential food resources in an estuarine system of Youngil Bay, Korea. Suspended particulate organic matter (POM) had distinct ${\delta}^{13}$C values from riverine (-31.8 to -27.2%$_o$) to marine waters (-21.0 to -16.6%$_o$). Estuarine macroalgae had a wide ${\delta}^{13}$C range of -22.8 to -15.0%$_o$. The ${\delta}^{13}$C values of riverine POM were more negative than that of riverine phytoplankton (-26.5 to -24.2%$_o$) but similar to that of freshmarsh plant species (-29.1 to -27.5%$_o$ for Phragmites communis and -28.5 to -27.0%$_o$ for Salix gracilistyla), These ${\delta}^{13}$C values suggest that the POM transported by the Hyungsan River is predominantly of terrestrial origin rather than riverine autochthonous sources. The ${\delta}^{13}$C values of Corbicula japonica tissues (-28.7 to -27.2%$_o$) were most similar to values for riverine POM and freshmarsh plants. There was no significant difference in the isotopic composition of the clam individuals. The results indicate a predominant contribution of organic carbon derived from terrestrial and fresmarsh plant detritus to the diet of Corbicula japonica. Our results also confirm previous suggestion that terrestrial organic matter can be incorporated into estuarine food webs although its role is confined to the upper estuarine reaches.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.50 no.6
• /
• pp.669-674
• /
• 2017

Paralytic shellfish toxins (PSTs) are produced by marine dinoflagellate phytoplankton Alexandrium spp. and Gymnodinium spp. These toxins accumulate in filter feeding organisms such as bivalves and the ingestion of contaminated shellfish can cause illness in humans. The mouse bioassay (MBA) has been the preferred PST testing method worldwide for more than 50 years. However, this assay has several disadvantages, such as detection limits, non-toxic-profiles, and the ethical issues of using animals. The aim of this study was to establish an alternative to the MBA method for testing for PSTs. We optimized the analysis conditions of a post-column oxidation-high performance liquid chromatography (PCOX-HPLC) method and the Scotia Rapid Test Kit, and then compared the accuracy of these methods to the MBA method. The results demonstrated a strong correlation between the PCOX-HPLC method and the MBA, although the PCOX-HPLC method required expensive equipment and standard material, and was time consuming. The Scotia Rapid Test Kit promises to be a useful tool, as it provided rapid and qualitative results, although the method sometimes gave a false positive result that could not be explained by toxin profiles.

• Ocean and Polar Research
• /
• v.34 no.2
• /
• pp.239-251
• /
• 2012

The food web dynamics in a coastal ecosystem of Korea were predicted with Ecosim, a trophic flow model, under various scenarios of primary productivity due to ocean warming and ocean acidification. Changes in primary productivity were obtained from an earth system model 2.1 under A1B scenario of IPCC $CO_2$ emission and replaced for forcing functions on the phytoplankton group during the period between 2020 and 2100. Impacts of ocean acidification on species were represented in the model for gastropoda, bivalvia, echinodermata, crustacean and cephalopoda groups with effect sizes of conservative, medium and large. The model results show that the total biomass of invertebrate and fish groups decreases 5%, 11~28% and 14~27%, respectively, depending on primary productivity, ocean acidification and combined effects. In particular, the blenny group shows zero biomass at 2080. The zooplankton group shows a sudden increase at the same time, and finally reaches twice the baseline at 2100. On the other hand, the ecosystem attributes of the mean trophic level of the ecosystem, Shannon's H and Kempton's Q indexes show a similar reduction pattern to biomass change, indicating that total biomass, biodiversity and evenness shrink dynamically by impacts of climate change. It is expected from the model results that, after obtaining more information on climate change impacts on the species level, this study will be helpful for further investigation of the food web dynamics in the open seas around Korea.

• Ocean and Polar Research
• /
• v.31 no.1
• /
• pp.97-110
• /
• 2009

Two of the most important topics of the 21st century are ensuring harmony between man and his environment and the emerging long-tail economy in which niche markets are becoming increasingly more important. Since the Industrial Revolution in 17th century, human beings have increasingly exploited the world's natural capital, such as the natural environment and its ecosystems. Now the world is facing limits to sustainable economic growth because of limits to this natural capital. Thus, most countries are beginning to adopt a new development paradigm, the so-called"Green Development Paradigm" which pursues environmental conservation in parallel with economic growth. Recently, the Korean government announced an ambitious national policy of Low Carbon & Green Growth for the next six decades. This is an important step that transforms the existing national policy into a new future-oriented one. The fisheries sector in particular has great potential for making a substantial contribution to this national policy initiative. For example, the ocean itself with its sea plants and phytoplankton has an enormous capacity for fixing carbon, and its vast areas of tidal flats have a tremendous potential for cleaning up pollutants from both the sea and the land. Furthermore, the fishing industry has great potential for the development of fuel-saving biodegradable technologies, and a long-tail economy based on digital technologies can do much to promote the production and consumption of green goods and services derived from the oceans and the fisheries. In order for this potential to be realized, the fisheries authority needs to develop a new green-growth strategy that is practical and widely supported by fishing communities and the markets, taking into account the need for greenhouse gas reduction, conservation of the ocean environment and ecosystems, an improved system for seafood safety, the establishment of strengthened MCS (monitoring control surveillance) system, and the development of coastal ecotourism. In addition, fisheries green policies need to be implemented through a well-organized system of government aids, regulations and compensation, and spontaneous (voluntary) orders in fishing communities should be promoted to encourage far more responsible fisheries.

• Journal of the korean society of oceanography
• /
• v.37 no.3
• /
• pp.154-159
• /
• 2002

Satellite remote sensing technology for ocean observation has evolved considerably in these last twenty years. Ocean color is one of the most important parameters of ocean satellite measurements. This paper describes a remote sensing of ocean color data project - Asian I-Lac Project; it also introduces several case studies using satellite images in the Asian waters. The Asian waters are related to about 30 Asian countries, representing about 60% of the world population. The project aims at generating long-term time series images (planned for 10 years from 1996 to 2006) by combining several ocean color satellite data, i.e., ADEOS-I OCTS and SeaWiFS, and some other sensors. Some typical parameters that could be measured include Chlorophyll- a (Chl-a), Colored Dissolved Organic Matter (CDOM), and Suspended Material (SSM). Reprocessed OCTS images display spatial variation of Chl-a, CDOM, and SSM in the Asian waters; a short term variability of phytoplankton blooms was observed in the Gulf of Oman in November 1996 by analyzing OCTS and NOAA sea surface temperature (SST)； Chl-a concentrations derived from OCTS and SeaWiFS have also been evaluated in coastal areas of the Taiwan Strait, the Gulf of Thailand, the northeast Arabian Sea, and the Japan Sea. The data system provides scientists with capability of testing or developing ocean color algorithms, and transferring images for their research. We have also analyzed availability of OCTS images. The results demonstrate the potential of long-term time series of satellite ocean color data for research in marine biology, and ocean studies. The case studies show multiple applications of satellite images on monitoring of coastal environments in the Asian Waters.