• YAKHAK HOEJI
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• v.37 no.3
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• pp.254-261
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• 1993

Developing new substance for immunosuppressor or immunomodulator from natural products is extremely important in the present biomedicine. In this paper, we focused our efforts on the mitogenicity and immunochemical properties of the two lectins (LCC-I, LCC-II) obtained from marine animal Lunella coronata coreensis. Immunochemical techniques were employed to elucidate the structural and/or functional similarities between the LCC lectins. Molecular weight of the LCC lectins, LCC-I and LCC-II were estimated to be around 60 KD and 66-70 KD, respectively. LCC lectins were mitogens for murine splenic lymphocytes, and the optimum mitogenic doses were 31.25 $\mu\textrm{g}$/ml and 3.91 $\mu\textrm{g}$/ml, respectively. LCC-II lectin was a good mitogen toward human peripheral lymphocytes at a concentration about 31.25 $\mu\textrm{g}$/ml.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.3
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• pp.455-462
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• 2003

Biotechnological research and development are moving at a very fast rate. The subject has assumed greatest importance in recent years in the development of agriculture and human health. The science of biotechnology has endowed us with new tools and tremendous power to create novel genes and genotypes of plants, animals and fish. The application of biotechnology in the fisheries sector is a relatively recent practice. Nevertheless, it is a promising area to enhance fish production. The increased application of biotechnological tools can certainly revolutionise our fish farming besides its role in biodiversity conservation. The paper briefly reports the current progress and thrust areas in the use of synthetic hormones in fish breeding, production of monosex, uniparental and polyploid individuals, molecular biology and transgenesis, biotechnology in aquaculture nutrition and health management, gene banking and the marine natural products.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.227-228
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• 2003

The most important of various merits are the capacity to investigate an total genome for polymorphism and AELP is superior to any other systems in terms of the number of sequences amplified per reaction and its reproducibility(Vos et al., 1995). The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of origin o. complexity (Vos et at., 1995). (omitted)

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.388-391
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• 2020

Sea stars were collected from the adjacent waters of Jukbyeon and Jumunjin, Korea, using fishing nets at depths of 70-100 m. The specimens were identified as Henricia perforata (O.F. Müller, 1776) in the family Echinasteridae and order Spinulosida, with worldwide species distribution. H. perforata can be distinguished from some morphologically related Henricia species by its long, slender, and pointed adambulacral spines. The abactinal skeleton of H. perforata has wide papular areas resembling those of H. pachyderma, but the former has more papulae (two to seven). Previously, two genera of Echinasteridae, Aleutihenricia and Henricia, with a total of 12 echinasterid species, have been reported in Korea. The morphological characteristics of H. perforata are described and photographs are provided.

• Korean Journal of Environmental Biology
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• v.39 no.3
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• pp.289-292
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• 2021

Henricia specimen was collected from the East Sea of Korea using fishing nets. The specimen was identified as Henricia hayashii (Djakonov 1961), belonging to the family Echinasteridae of the order Spinulosida. This species can be distinguished from other Henricia species by slender arms (R/r=7.4), constricted arm base, six to thirty-five slender spinelets, open-meshed, reticulated abactinal skeleton comprising semi-triangular, rod-like form, and small ossicles present inside the papular areas. This species superficially resembles H. reniossa in terms of its body size but differs in the shape and arrangement of the abactinal and actinal plates. To date, two genera of Echinasteridae, Aleutihenricia and Henricia, including a total of 14 species, have been reported in Korea. Herein, the morphological characteristics of H. hayashii are described, and photographs are provided.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.563-566
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• 2020

Henricia specimens were collected using fishing nets from the East Sea of Korea. The specimens were identified as Henricia oculata Pennant, 1777, belonging to the family Echinasteridae of the order Spinulosida. This species can be distinguished from other Henricia species by broad arms (R/r=4-4.1), rough skin, a thick arm base, three to nine minute delicate abactinal spines, and inferomarginal plates reniform in shape. This species superficially resembles H. pachyderma in its body size and wide papular areas but differs mainly in the number of papulae and abactinal spines, and the shape and arrangement of the inferomarginal plates. To date, two genera of Echinasteridae, Aleutihenricia and Henricia, with a total of 13 species, have been reported in Korea. The morphological characteristics of H. oculata are described, and photographs are provided.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.4
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• pp.289-293
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• 2008

Reproduction of the goby fish Periophthalmus magnuspinnatus was examined using 298 specimens collected from April to October 2005 in a mud flat of Suncheon Bay, Korea. Specimens ranged in body length(BL) from 1.3 to 9.1 cm. The gonadosomatic index(GSI) of females was highest in June and decreased until August. The hepatosomatic index(HSI) and fatness index of females were high in April and then decreased to nadirs in July. Spawning season lated from April to August and the ratio of females to males did not significantly differ($x^2$-test, p>0.05). The first spawning length was 4.5 cm BL, and the size of 50% maturity was estimated at 4.98 cm BL. Fecundity(F) ranged from 1,316 to 4,768 eggs, and the relationship between F and BL was estimated as $F=0.1562BL^{1.4068}(R^2=0.59)$.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.2
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• pp.272-277
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• 2012

Compensatory growth of grower olive flounder (Paralichthys olivaceus) was determined at suboptimal temperature ($13.0{\pm}1.9^{\circ}C$). Fifteen fish averaging 201.1 g per tank were distributed into 18 of 300 L flow-through tanks. Six treatments were prepared in triplicate: fish were hand-fed with an extruded pellet to apparent satiation once a day for 16 weeks (16 WF); and the other five groups of fish were hand-fed for 15, 14, 13, 12 and 10 weeks after 1-, 2-, 3-, 4- and 6-week feed deprivation, referred to as 15 WF, 14 WF, 13 WF, 12 WF and 10 WF, respectively. A linear relationship between body weight of fish and feed deprivation was observed: Y (Body weight of fish) = -1.81X (Weeks of feed deprivation)+201.07, $R^2$ = 0.83. Weight gain of grower olive flounder in 15 WF, 14 WF, 13 WF and 12 WF treatments was comparable to that of fish in 16 WF treatment, but lower than that of fish in 10 WF treatment. Specific growth rate of fish in 15 WF treatment was higher than that of fish in 16 WF, 14 WF and 10 WF treatments. Feed consumption of fish was not affected by feeding regime. Feed and protein efficiency ratios of fish in 15 WF treatment were higher than those of fish in 13 WF, 12 WF and 10 WF treatments. Grower olive flounder could achieve full compensatory growth when fish were daily fed for 12 weeks after 4-week feed deprivation at suboptimal temperature.

• Animal cells and systems
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• v.13 no.2
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• pp.133-139
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• 2009

Green tea seed was extracted with absolute ethanol,and the green tea seed extract(GTSE)was subjected to assays for toxicity, antioxidant ability, angiogenesis inhibitory effects and cell adhesion, as well as western blotting, and an in vivo experiment against 4 high-ranking adult cancers in Korea. Our series of experimental data demonstrated that GTSE has an antioxidant ability superior to that of EGCG in the green tea leaf, and also exhibits a profound high tumor growth inhibitory activity on a variety of cancer cell lines, as well as nude mice infected with cancer cells. GTSE was identified as a natural anticancer compound showing excellent angiogenesis inhibition and cancer cell suppression abilities. Our preliminary observations also indicate that GTSE may be another potential source of natural dietary antioxidants and also may be applicable as a novel natural anticancer agent.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.5
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• pp.647-653
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• 2015

A 12-wk feeding trial was conducted to evaluate the essentiality of choline supplementation in diets for parrot fish. Five isonitrogenous and isocaloric diets were supplemented with 0 (as control), 500, 1,000, and 2,000 mg choline per kg diet, and a positive control diet without choline contained 0.3% of 2-amino-2-methyl-1-propanol as choline biosynthesis inhibitor (designated as Con, C500, C1000, C2000 and $Con^+$, respectively). Triplicate groups of fish (body weight, $8.8{\pm}0.01g$) were fed one of the experimental diets at a rate of 4% body weight twice daily. The fish fed $Con^+$ diet revealed significantly lower growth performance and feed utilization efficiency than other fish groups. Supplementation of choline to the basal diet did not significantly influence fish growth. The highest liver lipid content was observed in fish fed the $Con^+$ diet and inversely correlated with liver choline concentration although the differences were not significant. Also, significantly higher liver linoleic, eicosapentaenoic and docosahexaenoic acid contents were found in fish fed the $Con^+$ diet. Innate immune parameters including respiratory burst and myeloperoxidase activities were not significantly affected by dietary choline levels. The findings in this study conclude that choline concentration of approximately $230mgkg^{-1}$ diet meets the requirement of parrot fish.