• 한국수산과학회지
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• 제34권5호
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• pp.502-508
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• 2001

병원성 비브리오 속인 Vibrio furnissii의 용혈소를 분리정제하고 그 물리화학적 특성, 생물학적인 그리고 면역학적인 상호관련성을 검토하여, 다음과 같은 결과를 얻었다. 용혈소의 정제는 ammonium sulfate precipitation, DEAE-Sephadex A-50 (1st와 2nd)와 Sephaedx G-100 gel permeation chromatography를 거쳐 738배로 정제되었으며 회수율은 각각 $11.6\%$ 와 $5.2\%$로 나타났다 전기영동법으로 용혈소의 정제를 확인한 결과 단일 밴드의 형성을 관찰할 수 있었다. 분자량은 61 kDa이였으며 또한 SDS-PAGE 결과 subuint는 존재하지 않았다. 정제된 용혈소는 열에 불안정한 이열성 용혈소이며 $Cu^{2+},\;Zn^{2+}$와 cholesterol에 의해 용혈활성이 모두 저해되었다. 용혈기작에 있어서는 온도의존성 용혈기작으로서 최적 용혈의 온도는 $37^{\circ}C\sim47^{\circ}C$로 나타났다. 정제용혈소의 pH 안정성은 pH 6.0, 6.5, 10.0에서 각각 불안정하였다. 용혈활성에 있어서 최적 혈구 농도는 $1.0\sim2.0\%$였다. 또한 각종 동물 혈구의 감수성 시험 결과 두 용혈소 모두 토끼에서 가장 감수성이 높았으며 사람의 혈구에서는 B형 혈액형에서 다소 높은 감수성을 나타내었다.

• Animal Systematics, Evolution and Diversity
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• 제28권3호
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• pp.192-207
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• 2012

To supply the morphological and ecological information of oligotrich ciliates in Korea, water samples were seasonally collected in Gwangyang Bay and Jinhae Bay from August, 2010 to February, 2012 and processed by quantitative protargol staining method. As a result, six species belonging to the genus Strombidium Clapar$\grave{e}$de and Lachmann, 1859 are identified: Strombidium emergens Kahl, 1932; S. dalum Lynn et al., 1988; S. epidemum Lynn et al., 1988; S. tressum Lynn et al., 1988; S. bilobum Lynn and Gilron, 1993; S. pollostomum Lynn and Gilron, 1993. These six species were newly reported from Korean coastal waters. Strombidium emergens is a middle sized Strombidium ($20-50{\mu}m$ in length) and has open and deep oral groove to girdle portion. Strombidium dalum is a small sized Strombidium (${\geq}20{\mu}m$ in length), that has torch-like spiral anterior membranelles and an inverted triangles-shaped macronucleus on the posterior pole. The small sized S. epidemum has conspicuous trichites surrounding the girdle portion and ventral membranelles distinctly separated from anterior membranelles. The small sized S. tressum has torch-like spiral and extremely long trichites among the cilia of anterior membranelles. The middle sized S. bilobum has a bilobed macronucleus. Strombidium pollostomum is also a small sized Strombidium but its ventral membranelles are continuously connected with anterior membranelles. The five species except S. emergens occurred frequently over the wide range of water temperatures and salinities.

• 한국양식학회지
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• 제11권3호
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• pp.401-419
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• 1998

제주도의 8개지점(함덕, 온평, 위미, 법환, 화순, 대포, 한림, 외도)에서 해조장형성과 조식성의 대표적이 동물인 성게류(보라성게,Anthocidaris crassispina와 말똥성게 Hemicentrotus pulcherrimus)의 섭이형태에 대하여 연구하였다. 각 조사지점의 해조류 생체량이 조사되었으며, 성게류의 장내용물과 생물학적 측정치에 관련된 해조류 군락의 양적분포와 우점도가 조사되었다. 결론적으로 대포지점의 조사지점이 해조상으로는 가장 우세함에도 불구하고 성게의 생물학적인 특성인 체고, 체장, 체중 그리고 생식소 지수와의 관련이 없었다. 이는 성게류들이 조류의 풍도에만 의존하는 곳이 아니고 다른 먹이물질을 섭이함을 뜻한다. 성게의 음식물 선호도를 위해 섭이된 장내용물을 조사한 결과 부착성 조류와 유조(drifted algae) 및 다양한 동물들 (해면, 태충류, 갑각류 및 미확인된 캡슐에 싸인 알 등)과 펄이나 모래와 같은 저질들이 먹이물질로 조사되었다. 섭이된 음식물의 상대적인 양으로 볼 때, 해조류의 섭식률은 낮은 것으로 밝혀졌으며, 이로서 성게가 조장형성이나 갯녹음 현상을 일으키는 해조류의 우요 섭식자가 아닐 것으로 추정할 수 있다.

• 한국어류학회지
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• 제21권sup1호
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• pp.29-37
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• 2009

The tiny fragment of freshwater body is providing home for huge biodiversity and resources for the existence of human. The competing demand for freshwater have been increased rapidly and it caused the declination of biodiversity in recent decades. Unlike the natural process of extinction in gradual progress, the current species extinction is accelerated by human activity. As a result many fish species are already extinct or alive only in captivity in the world and about fifty eight animal species are in endangered in Korea including eighteen freshwater species. Conservation of biodiversity is the process by which the prevention of loss or damage is attained, and is often associated with management of the natural environment. The practical action is classified into in-situ, or ex-situ depending on the location of the conservation effort. Recovery means the process by which the status of endangerment is improved to persist in the wild by re-introduction of species from ex-situ conservation population into nature or translocation of some population. However there are a lot of restrictions to complete it and successful results are known very rare in case. In this article the authors explore some strategies for conservation and restoration of freshwater fish species conducted in Korea for few years. The major causes are discussed in relation with the decline of freshwater fish diversity during few decades and some strategies are evaluated to advance the process of conservation. A study on the Korean bullhead, Pseudobagrus brevicorpus, is introduced as a case for ex-situ conservation and restoration in freshwater ecosystem.

• 한국수산과학회지
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• 제49권1호
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• pp.38-44
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• 2016

The quality and quantity of live food sources strongly influence the success of fish production in farming operations. Thus, critical studies of live forage species are a crucial element for progress in fish aquaculture. The fat content of food is an especially important determinant of growth in marine fish. Omega-3 highly unsaturated fatty acids (HUFA) are essential components of diet that determine the nutritional value of larval fish. Euglena is a protist that has potential as a forage species. These single-celled organisms have plant and animal characteristics they are motile, elliptical in shape and 15–500 μm in diameter. Their nutritional content is excellent, but most studies have focused on cells raised in autotrophic culture. We therefore examined differences in the lipid and fatty acid contents, and the growth of Euglena cells grown under autotrophic, heterotrophic, and mixotrophic conditions. Biomass production reached 15.03 g/L, 12.28 g/L, and 3.66 g/L under mixotrophy, heterotrophy, and autotrophy, respectively. The proportional n-3 HUFA content differed among culture methods: 10.04%, 5.80% and 10.01% in mixotrophic, heterotrophic and autotrophic cultures, respectively. Mixotrophy was to be the best form of cultivation for improving the growth and nutritional content of Euglena.

• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.135-144
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• 2015

Heat shock protein (HSP) 70, the highly conserved stress protein families, plays important roles in protecting cells against heat and other stresses in most animal species. In the present study, we identified and characterized four Hsp70 (RuHSP4, RuHSC70, RuHSP12A, RuGRP78) family proteins based on the expressed sequence tag (EST) analysis of the Korean rose bitterling R. uyekii cDNA library. The deduced RuHSP70 family has high amino acid identities of 72-99% with those of other species. Phylogenetic analysis revealed that RuHsp70 family clustered with fish groups (HSP4, HSC70, HSP12A, GRP78) proteins. Quantitative RT-PCR analysis showed the specific expression patterns of RuHsp70 family members in the early developmental stages and several tissues in Korean rose bitterling. The expression of 4 groups of Hsp70 family was detected in all tested tissue. Particularly, Hsp70 family of Korean rose bitterling is highly expressed in hepatopancreas and sexual gonad (testis and ovary). The expression of Hsp70 family was differentially regulated in accordance with early development stage of Rhodeus uyekii.

• Animal Systematics, Evolution and Diversity
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• 제27권1호
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• pp.25-33
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• 2011

Three morphospecies of the genus Uronychia, i.e. U. setigera Calkins, 1902, U. binucleata Young, 1922, and U. multicirrus Song, 1997, were collected from the coastal waters of Gumjin-ri on the East Sea and the public waterfront of Incheon on the Yellow Sea in Korea, respectively. These species are described based on live observation, protargol impregnation, silver nitrate impregnation, and their morphometrics. Diagnostic keys for these species are also provided. In addition, their small subunit ribosomal DNA sequences were compared with previously known sequences of Uronychia species. Diagnostics of three Uronychia species are as follows: U. setigera: $50-80\;{\mu}m$ long in vivo, oval-shaped, 2 macronuclear nodules (Ma), 1 spur on the left margin, 11 adoral membranelles (AM) 1, 4 AM2, 1 buccal cirrus (BC), 4 frontal cirri (FC), 3 left marginal cirri (LMC), 2 ventral cirri (VC), 5 transverse cirri (TC), 3 caudal cirri (CC), 6 dorsal kineties (DK), and approximately 23 cilia in the leftmost kinety. U. binucleata: $70-110\;{\mu}m$ long in vivo, oval to slightly rectangular shaped, 2 Ma, 1 micronucleus (Mi), 2 spurs on the posterior region, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, 2 VC, 5 TC, 3 CC, 6 DK, and approximately 37 cilia in the leftmost kinety. U. multicirrus: $140-200\;{\mu}m$ long in vivo, oval to slightly rectangular shaped, ca. 7 Ma, 1 Mi, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, approximately 8 VC, 5 TC, 3 CC, and 6 DK. This study presents the first record of this genus in Korea.

• Animal Systematics, Evolution and Diversity
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• 제28권4호
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• pp.279-290
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• 2012

Plecoglossus altivelis (ayu) is an amphidromous fish widely distributed in Northeastern Asia from the East China Sea to the northern Japanese coastal waters, encompassing the Korean Peninsula within its range. The shore lines of northeastern region in Asia have severely fluctuated following glaciations in the Quaternary. In the present study, we investigate the population genetic structure and historical demographic change of P. altivelis at a population level in East Asia. Analysis of molecular variance (AMOVA) based on 244 mitochondrial control region DNA sequences clearly showed that as the sampling scope extended to a larger geographic area, genetic differentiation began to become significant, particularly among Northeastern populations. A series of hierarchical AMOVA could detect the genetic relationship of three closely located islands between Korea and Japan that might have been tightly connected by the regional Tsushima current. Neutrality and mismatch distribution analyses revealed a strong signature of a recent population expansion of P. altivelis in East Asia, estimated at 126 to 391 thousand years ago during the late Pleistocene. Therefore it suggests that the present population of P. altivelis traces back to its approximate demographic change long before the last glacial maximum. This contrasts our a priori expectation that the most recent glacial event might have the most crucial effect on the present day demography of marine organisms through bottleneck and subsequent increase of effective population size in this region.

• Animal Systematics, Evolution and Diversity
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• nspc8호
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• pp.1-176
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• 2010

Siphonostomatoid copepods associated with marine invertebrates are described from tropical waters of the West Indies and Madagascar. They belong to the families Asterocheridae (7 new genera and 39 new species), Dinopontiidae (one new species), and Nanaspididae (one new species). New taxa of the Asterocheridae are 14 species of Asterocheres (Asterocheres unioviger n. sp., A. trisetatus n. sp., A. bahamensis n. sp., A. tricuspis n. sp., A. plumosus n. sp., A. peniculatus n. sp., A. oricurvus n. sp., A. planus n. sp., A. sensilis n. sp., A. indivisus n. sp., A. nudicoxus n. sp., A. tenuipes n. sp., A. galeatus n. sp., and A. fastigatus n. sp.); 3 species of Hetairosynella n. gen. (Hetairosynella bifurcata n. sp.; H. angulata n. sp.; H. aculeata n. sp.); 15 species of Asteropontius (Asteropontius capillatus n. sp., A. membranulatus n. sp., A. plumatus n. sp., A. parvipes n. sp., A. humesi n. sp., A. angulatus n. sp., A. latioriger n. sp., A. magnisetiger n. sp., A. pinnatus n. sp., A. trifilis n. sp., A. orcafer n. sp., A. bilinguis n. sp., A. dentatus n. sp., A. minutisetiger n. sp., and A. bispinifer n. sp.); Collocherides minutus n. sp.; Cyclocheres sensilis n. gen. n. sp.; Stenomyzon edentatum n. gen. n. sp.; Cephalocheres flagellatus n. gen. n. sp.; Humesimyzon pusillum n. gen. n. sp.; Thermocheres validus n. gen. n. sp.; and Gascardama longisiphonata n. gen. n. sp. New taxa of the remaining two families are Stenopontius spinulatus n. sp. in the Dinopontiidae and Stephopontius ahni n. sp. in the Nanaspididae. Asterocheres crinoidicola Humes, Asteropontius genodon Stock, and Asteropontius ungellatus Stock are redescribed. Asteropontius gonioporae Kim is reported as a new record from Madagascar. A key to species of Asteropontius is provided.

• 한국실내디자인학회논문집
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• 제20권5호
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• pp.125-134
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• 2011

Art Deco is a decorative and eclectic design style, popularized at the interwar period. The term Art Deco derives from the Exposition Internationale des Arts D$\acute{e}$coratils et Industriels Modernes held in Paris in 1925. The aim of the exposition was to create new modern aesthetics. This exposition introduced the modern decorative and industrial art to the world and influenced all designers of area, including architects, interior designers, industrial designers, craftsmen, fashion designers, etc. Art Deco designers applied inspirations from a variety of sources and movements such as the Cubist abstract, the Neoclassical refinement, Egyptian exotic elements, Babylonian and Aztec temples, the machine aesthetic, avant-garde movements, etc to their modern works. Art Deco style rapidly spread all over the design areas nationwide in America. In Art Deco architecture, in particular, its inception was French but its domination was American. Skyscrapers, airplanes, automobiles, ocean liners, jazz, Hollywood film, streamline, and native Indian symbols are the defining features of American Art Deco. This study began from questions on how these features are expressed and stylized to decoration elements as the modern aesthetics in American Art Deco architecture. Thus, the purpose of the study is to find out the ornamental and eclectic factors of Art Deco style and to define a concept of the modernity and the regionalism of Art Deco architecture in America. This article provides an overview of the decoration style of Art Deco architecture in America through the analysis of ornamental and eclectic factors reflecting diverse roots. It also analyzes the wide variety of building examples of American Art Deco which represent regionalism. In addition, this study focuses on Art Deco architecture in Miami, Florida. Miami is one of typical cities that has the most unique regional aspects of 1920's to 1940's in Art Deco architecture. Miami Art Deco architecture reveals the tropical and nautical references such as streamlined and curved walls, exotic animal motifs, flora and fauna motifs, and marine motifs: use of glass block, porthole window, terra-cotta, and pastel color stucco.