• Animal Systematics, Evolution and Diversity
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• 제9권2호
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• pp.261-280
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• 1993

1993년 6월 28일부터 7월 3일 까지 문섬, 범섬 및 숲섬에서 채집된 히드라충류는 10과 25속 46종/아종으로 동정, 분류되었다. 이 중 뿌리히드라(Rhizocaulus chinenesis), 쌍뾰족혹히드라(Obelia bicuspidata), 사슬화관히드라(Sertularia desmoides), 가시깃히드라(Plumularia spinulosa)의 4종은 제주 미기록종이고, 기생털히들(Hebella parastica), 그린란드종히들 (Campanularia groelandica), 나팔종히드라(Campanularia everta), 나탈테히드라(Sertularella natalensis)의 4종은 한국미기록종으로 밝혀졌다. 문헌기록에 의하면 제주도 해산 히드라충류는 12과 41속 91종/아종이 된다. 제주해역의 우점종은 자색깃히드라(Aglaophenia suensonii)이다. 채집기별 종의 분포를 서귀포에 71종(78%)이 분포하여 가장 높은 분포를 나타냈고 그 다음이 문섬 43종(47%), 숲섬 26종(29%), 위미리 13종(14%), 범섬 11종(12%)의 순으로 나타났다. 제주해역에만 분포하는 종은 30종(33%)으로 밝혀졌다.

• Animal cells and systems
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• 제16권1호
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• pp.77-84
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• 2012

The objective of the study was to investigate if reproductive characteristics of $Pandalus$ $eous$ affect the depth distribution in the East Sea of Korea. $P.$ $eous$ was found at depths of 500-900 m in the East Sea of Korea, with the highest percentage occurrence (34%) at 500 m. A negative correlation was observed between the number of individuals and the depth. The overall sex ratio also turned out to be significantly correlated with depth. On average, the larger individuals (bigger than 26.37 cm), which included transitional, female, and ovigerous females, were mostly distributed at 700 m depth. The percentage of males increased by depth and ovigerous females were mainly distributed in the shallow water (300 m) during winter. Ovigerous females were not found at 900 m, which is the deepest depth range in this study. The percentage of transitional individuals was greatest at 500 m and decreased gradually with depth. All ovigerous female individuals were of the spent ovarian stage in winter. Female numbers in the ripe ovarian stage increased with depth and immature females rarely appeared. The gonadosomatic indices of the nonovigerous females and ovigerous females were highest at 700 m in depth. The mean egg size of $P.$ $eous$ was $0.83{\pm}0.11mm^3$ in the non-eyed stage and $0.93{\pm}0.17mm^3$ in the eyed stage.

• Animal cells and systems
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• 제13권4호
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• pp.447-452
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• 2009

We cloned the complete complementary DNA (cDNA) of a Pacific oyster (Crassostrea gigas) cytochrome P450 (CYP450)-related protein using rapid amplification of cDNA ends (RACE). The cDNA included a 1470 bp open reading frame that began with the first ATG codon at position 103 bp and ended with a TAG stop codon at position 1573 bp (GenBank accession EF451959). The sequence had all major functional domains and characteristics of previously characterized CYP450 molecules, including the heme-binding region (FGVGRRRCVG) and putative arginine codon (R) integral to enzymatic function. An NCBI/GenBank database comparison to other CYP450 genes revealed that the deduced C. gigas CYP450 amino acid sequence is similar to that of mouse (Mus musculus) CYP450 2D/II (28%, accession AK078880), rabbit (Oryctolagus cuniculus) CYP450 2D/II (28%, AB008785), and white-tufted-ear marmoset (Callithrix jacchus) CYP450 2D (28%, AY082602). Thus, although the C. gigas CYP450 we cloned appears to belong to the 2D type of the CYP450 group, it has low similarity to this type. CYP450 mRNA expression increased over 6 h in C. gigas gills at $30^{\circ}C$ and $10^{\circ}C$, and then decreased, indicating that CYP450 plays an important role in C. gigas exposed to water temperature changes. This finding can be used as a physiological index for Pacific oysters exposed to changing water temperatures.

• Animal cells and systems
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• 제13권4호
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• pp.439-445
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• 2009

Cytochrome P450 aromatase (P450arom) catalyzes the conversion of androgens to estrogens and plays an important role in reproduction and development in vertebrates. We investigated the expression patterns of ovarian P450arom (P450aromA) and brain P450arom (P450aromB) mRNA during sex change in black porgy. Maturity was divided into seven stages from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary, and mature ovary). P450aromA expression was significantly higher in the ovarian portion of mostly-ovarian stage fish, and P450aromB expression was highest in the brain of black porgy with mostly-ovarian gonads. Histology showed that testicular tissues were disintegrated with the development of ovarian tissue associated with an increase in the expression of the two P450arom mRNAs during sex change. Interestingly, among various tissues, P450aromA was only expressed in the ovary, and P450aromB was only expressed in the brain. To understand the role of gonadotropin-releasing hormone (GnRH) and estradiol ($E_2$), we injected exogenous hormone (GnRH analogue [GnRHa] and $E_2$) into immature black porgy. In the GnRHa group, expression of the two P450arom genes decreased 12 h after injection, and expression of the two P450arom genes were significantly higher at 6 dafter $E_2$ injection. These results provide useful baseline knowledge on the mechanism of natural sex change in black porgy.

• Current Optics and Photonics
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• 제1권2호
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• pp.132-137
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• 2017

To investigate the usefulness of optical coherence tomography (OCT) for imaging lymphedema, we directly compared it to other histological methods in a mouse model of lymphedema. We performed detailed imaging of the lymphedema lesion on a mouse tail. We imaged the mouse tail in vivo with OCT and created histopathological samples. We constructed a spectrometer-based OCT system using a fiber-optic Michelson interferometer. The light was directed to 50:50 couplers that split the light into reference and sample arms. Backscattered light from a reference mirror and the sample produced an interference fringe. An OCT image of the lymphedema model revealed an inflammatory reaction of the skin that was accompanied by edema, leading to an increase in the light attenuation in the dermal and subcutaneous layers. Similar to OCT image findings, histological biopsy showed an inflammatory response that involved edema, increased neutrophils in epidermis and subdermis, and lymphatic microvascular dilatation. Furthermore, the lymphedema model showed an increase in thickness of the dermis in both diagnostic studies. In the mouse tail model of lymphedema, OCT imaging showed very similar results to other histological examinations. OCT provides a quick and useful diagnostic imaging technique for lymphedema and is a valuable addition or complement to other noninvasive imaging tools.

• Animal cells and systems
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• 제12권4호
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• pp.305-311
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• 2008

Age and growth of the mudskipper, Scartelaos gigas were investigated using the second actinost bone of the pectoral girdle, based on an analysis of 560 individuals collected from the mud flats in south west Korea. Specimens were collected semimonthly from March to September of 2003. Actinost and ring radii of each ring group showed a direct one-to-one relationship, with ring radius increasing in tandem with actinost radius. Monthly change in the marginal indices showed that ring formation occurred between May and July(mainly June), being supported by the monthly change in the gonadosomatic(highest in June) and hepatosomatic(lowest in July) indices. Because the species has not been found since November, when air temperature was less than about $10^{\circ}C$, it is thought to enter to hibernation. Therefore, it was suggested that the two new actinost rings may appear a year: one during the spawning season(May-July) and another during hibernation(since November). Although the growth of S. gigas must be limited to short periods each year from March to April and from August to October, its growth rate was considerably fast. The von Bertalanffy growth parameters of S. gigas were $TL_{\infty}$=179.36 mm, K=0.78 $year^{-1}$, $t_0$=-0.7762, and age-length key were $TL_1$=134.3 mm, $TL_2$=158.6 mm, $TL_3$=169.1 mm, $TL_4$=175.0 mm.

• 수산경영론집
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• 제24권2호
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• pp.1-16
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• 1993

Because of the radical changes in the domestic and foreign economic circumstances Koreaa fisheries is confronted with difficulties. Along the end of the UR marine products of other nations are rushing into Korea. Also migration of labor to other industries and rise of wage level in Korean fisheries deteriorate managerial conditions. But in Korea which has little natural resourses fisheries is still more important. That is \circled1 creating job opportunites \circled2 increase of income \circled3 supply of foodstuffs and animal protein \circled4 acquisition of foreign currency \circled5 enlargement of domestic market for industrial products \circled6 development of other interrelated industries \circled7 rational use of domestic resourses \circled8 diversification of population and production activites \circled9 contribution to balanced growth of national economy by the developing regional economy. These roles of fisheries in national economy mentioned above are to be excuted forward. In spite of the radical change in the economic circumstances at the end of the UR fisheries if crucial in Korea as a industry. For this our fisheries is to be made to compete with foreign fisheries. Through the cheap price and high quality our fisheries must be came to compete with foreign fisheries and meet the people's needs for marine products. For this it is necessary to maintain high productivity and competitive power. Now with the exception of a portion of the deep - sea fishing, our fisheries is generally paltry, Especially inshore fishery which is the main stock in our fisheries is very paltry and so productivity and competetive power are very low. So to develop our fisheries which has s comparative disadvantage active polices that follows below are to be promoted on a large scale. \circled1 improvement of structure \circled2 augmentation of productivity in fishing ground by making fisheries resourses \circled3 enlargement of finantial and monetary assistance \circled4 effective administration of fisheries cooperative union \circled5 activation of R&D etc These polices which need to be scientific and comprehensive are very valuable. Especially without making fishieries resources we cannot expect economy of scale, promotion of productivity and development of fisheries. And we do also endeavour to gather the results of the study and investigation about fisheries domestic and foreign and do ceaselessly put these to practical use systimatically.

• Animal cells and systems
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• 제14권2호
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• pp.137-145
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• 2010

Four specimens of unknown Coilia sp. were collected for the first time from the Yellow Sea in 2008 and compared with Coilia mystus and Coilia nasus. Coilia sp. showed similar morphology to C. mystus and C. nasus, but differed in that its tail was considerably shorter. We conducted an analysis of the morphological and genetic characteristics in an effort to clarify the taxonomic position of Coilia sp. In counts and measurements, Coilia sp. were well distinguished from C. nasus by the number of scutes (42-44 in Coilia sp. vs. 40-45 in C. mystus vs. 45-55 in C. nasus), ratio of dorsal base length to head length (43.4-47.6 vs. 37.9-47.6 vs. 33.0-41.0), and eye length to head length (19.2-20.8 vs. 17.0-22.4 vs. 13.8-18.2). In caudal skeleton of Coilia sp., urostyle, hypural and epural bones were not observed; instead of them, caudal fin rays were supported by the last vertebra, neural and haemal spines' extension. The molecular phylogenetic relationship was analyzed using 414 base-pair 12S rRNA mitochondrial DNA sequences. The Kimura-2-parameter distance between Coilia sp. and C. mystus was 0.3%, but was 1.3% between Coilia sp. and C. nasus. Both the neighbor-joining tree and maximum-likelihood tree showed that Coilia sp. are closely clustered with C. mystus. Therefore, our results suggest that the Coilia sp. may be a deformed fish of C. mystus.

• 한국발생생물학회지:발생과생식
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• 제13권1호
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• pp.43-49
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• 2009

We examined the effects of the vibration stress on cortisol secretion and hematological characteristics in soft-shelled turtle, Pelodiscus sinensis. For the stressed group vibration of $45{\sim}78 dB(V)$ from electric vibrator applied for 30 min with 2-h intervals during daytime ($08:00{\sim}18:00$) up to 28 days. Using the blood samples collected from ten turtles held once a week after vibration stress, we measured hematocrit, hemoglobin, red blood cells, cortisol, glucose, lactic acid, osmolality, $Na^+,\;K^+,\;Cl^－$, aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results have showed that P. sinensis received vibration stress exhibit the 'typical' stress-induced physiological responses (cortisol, glucose, lactic acid, osmolality, ions, hematocrit and hemoglobin) induced by vibration stress. Our data suggested that chronic vibration stress caused substantial stress in the animal, and in particular, the persisting elevated levels of AST and ALT would be highly correlated with the adverse effects of the stress. The high hematological characteristics during entire experimental period showed that the P. sinensis could not adapt to chronic stimuli provoked by vibration stress.

• 한국해양바이오학회지
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• 제3권1호
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• pp.7-12
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• 2008

Cameroon is a country in Western Africa with 16 million inhabitants. Located between Nigeria and Equatorial Guinea, the country is bordered by the Bight of Biafra. It has a 402 km long coastline. It covers an area of about 475,440 sq km. Cameroon obtains its fish supply from five distinct sources notably-small scale maritime Fisheries, inland fisheries, industrial fisheries, aquaculture and importation. Despite its enormous potential, Cameroon produces only 180,000 metric tons fish annually. The total artisanal annual catch is estimated to be 55,000 t of which bonga/Sardinella, white shrimp and demersal fish contribute 58%, 27% and 15% respectively. The industrial fleet expanded rapidly during the sixties and by 1973 there were 29 trawlers and 13 shrimpers which landed a total of about 17,600 t of fish and shrimp. The total catch of the industrial fisheries peaked at about 20,400 t in 1976 and since then catches have generally declined. The per capita fish consumption in Cameroon stands at about 17.9 kg per inhabitant per year. This means that the demand of fish in Cameroon stands at about 280,000 tons. There is a deficit of in fish supply for which the government always resort to massive importation of fish products to meet the local demand. The main fish product for export from Cameroon is the prawn (Panacus duorarum). The main export market of Cameroon's prawn is the European Union (EU). Between 1998 and 2003, Cameroon's export value has been on the decline, dropping from 1,836 metric tons of prawns for a value of 264 millions USD in 1998 to 51 tonnes, for an estimated value of 315,000 USD in 2003. This drastic drop in exportation is consequential to Cameroon's auto suspension from the exportation of prawns towards the EU as a result of non compliance with EU standards. Today, a good quantity of the catches is being exported illicitly through neighbouring countries.