• Animal cells and systems
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• 제14권2호
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• pp.115-123
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• 2010

The complete larval development of Rhinolithodes wosnessenskii Brandt, 1848 is described, based on laboratory rearing. The species has four zoeal stages and a megalopa. The zoeas of R. wosnessenskii are readily distinguished from all other described zoeas of the subfamily Lithodinae by having a middorsal spine of the posterior margin of the carapace.

• Animal Systematics, Evolution and Diversity
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• 제14권3호
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• pp.219-228
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• 1998

1994년부터 1998년까지 거제도에서 어망과 잠수부에 의해서 채집된 해면류는 15과 25속 34종으로 동정, 분류되었다. 이중 Tedania rhoi 로이테다니해면은 신종으로 밝혀졌고, Clathria(Axociella) simae 둥근축털해면은 한국 미기록종으로 판명되었다.

• Animal Systematics, Evolution and Diversity
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• 제20권1호
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• pp.45-54
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• 2004

A new species of marine Halacaridae, Copidognathus laevisetosus is recorded from Yeongdeok coast, Korea. In morphological aspects, the present new spe챠es is closely related to C. koreanus Chatterjee and Chang. Morphological similarities and dissimilarities between them are discussed. Copidognathus cerberoideus Bartsch is reported here for the first time from Korea and away from its type locality.

• Animal Systematics, Evolution and Diversity
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• 제37권4호
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• pp.280-286
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• 2021

During a field survey on Korean coastal marine waters, two ciliates belonging to the genera Pseudovorticella Foissner & Schiffmann, 1975 and Frontonia Ehrenberg, 1838 in the class Oligohymenophorea de Puytorac et al., 1974, were collected. Even though the two genera are very common in the aquatic ecosystems, they had been superficially studied in Korean habitats. Using the observation of living cells and specimens impregnated by protargol, silver carbonate, and silver nitrate, two newly recorded ciliate are identified as P. paramarina Sun, Ji, Warren & Song, 2009 and F. ocularis Bullington, 1939. A brief diagnosis, remark, and comprehensive micrographs are provided for each species.

• Animal Systematics, Evolution and Diversity
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• 제22권2호
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• pp.153-168
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• 2006

A taxonomic study on the marine sponges was conducted with the materials collected from Chujado Island, Korea during the period June 2001 to May 2005 by SCUBA and hands. They were classified into 63 species, 31 genera, 25 families, seven orders in a class. Among them, Antho (Arcania) manarensis Carter, 1880 and Eurypon lendenfeldi Hentschel, 1912 were newly added to Korean Fauna. Two species, Biemna chujaensis and B. jeolmyongensis, were new to science.

• Ocean and Polar Research
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• 제40권2호
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• pp.69-75
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• 2018

We investigated the effects of environmental variations on the growth and survival rate of Stichopus japonicus to determine the optimum environmental conditions for its growth. Literature studies and a 12 month-long diver survey were carried out to understand the habitat, ecology and size of the surveyed area. Based on the collected data, we suggested optimum habitat conditions for releasing S. japonicus. Experiments on releasing S. japonicus were conducted in the breakwater of the Hwagye fishing cooperative in Hwagey-ri, Namhae-un, Gyeongsangnam-do, Korea. To implement the experiments, we divided the surveyed area into 4 sub-areas with different characteristics: (1) sand and silt zone; (2) artificial sea cucumber bank zone; (3) artificial rock bank zone; and (4) marine algae zone. The experiment lasted for 12 months. We released 32,000 sea cucumbers over $120m^2$ of each of the sand and silt zone, artificial rock bank zone and marine algae zone and released 6,000 sea cucumbers over $120m^2$ of the artificial sea cucumber bank zone. The average density of the released sea cucumbers from day 30 to day 360 after the releasing was conducted was the highest in the artificial sea cucumber bank zone ($23.7animal/m^2$), which was followed by artificial rock bank zone ($2.0animal/m^2$), marine algae zone ($1.9animal/m^2$) and sand and silt zone ($0.8animal/m^2$). The analysis on growth showed that the initial average weight of 2.3 g increased on day 360 after the releasing to 12.5 g in the artificial sea cucumber rank zone, 20.2 g in the sand and silt zone, 23.3 g in the artificial rock bank zone and 22.9 g in the marine algae zone. Results from the experiment along with the literature analysis suggest the following optimum habitat conditions: $10-15^{\circ}C$ water temperature; 28-34 psu salinity; 5-10 m water depth; 0.2-0.5 m/s velocity; rock, stone and muddy sand as substrate; and less than 20% mud in the substrate.

• Fisheries and Aquatic Sciences
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• 제25권6호
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• pp.327-334
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• 2022

Marine resources have various biological activities and their constituents are more novel than those of land organisms. Several biologically active constituents have been found in marine organisms. Recently, many studies have reported that marine animals (MAs) can be used as functional ingredients in functional foods or nutraceutical due to their health benefits. However, no studies have extensively investigated the cosmeceutical activities of MAs extracts. Here, 70% ethanol extracts of 38 MAs were investigated for their activities of whitening and anti-aging properties for use as materials in novel cosmeceuticals. Anti-aging activities were determined by skin aging-related enzyme activities (anti-collagenase, anti-elastase, anti-hyaluronidase) and whitening activities (anti-tyrosinase, anti-3,4-dihydroxyl-L-phenylalanine [DOPA] oxidation) evaluated by colorimetric method. Among the 38 MAs, we found that Urechis unicinctus and Petrosia corticata extracts showed the strongest inhibitory effects against tyrosinase and DOPA oxidation, respectively. Our results additionally showed that Protankyra bidentata extract might provide a major source of anti-hyaluronidase and anti-elastase; meanwhile, anti-collagenase effects were similar in most MAs. Overall, these results suggest that extracts of marine animals have potential as a tyrosinase, collagenase, elastase, and hyaluronidase inhibitors. Taken together, MA resources could be considered as a novel cosmeceutical agent to be applied in cosmetic industry.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1582-1588
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• 2005

This study aimed to evaluate the effects of silage additives on the fermentation qualities and residual mono- and disaccharides composition of silages. Forage Oats (Avena sativa L.) and Italian Ryegrass (Lolium multiflorum Lam.) were ensiled with glucose, sorbic acid and pre-fermented juice of epiphytic lactic acid bacteria (FJLB) treatments for 30 days. In both species grass silages, although the respective controls had higher contents of butyric acid (20.86, 33.45g $kg^{-1}$ DM) and ammonia-N/total nitrogen (100.07, 114.91 g $kg^{-1}$) as compared with other treated silages in forage oats and Italian ryegrass, the fermentation was clearly dominated by lactic acid bacteria. This was well indicated by the low pH value (4.27, 4.38), and high lactic acid/acetic acid (6.53, 5.58) and lactic acid content (61.67, 46.85 g $kg^{-1}$ DM). Glucose addition increased significantly (p<0.05) lactic acid/acetic acid, and significantly (p<0.05) decreased the values of pH and ammonia-N/total nitrogen, and the contents of butyric acid and volatile fatty acids as compared with control, however, there was a slightly but significantly (p<0.05) higher butyric acid and lower residual mono- and di-saccharides as compared with sorbic acid and FJLB additions. Sorbic acid addition showed the lowest ethanol, acetic acid and ammonia-N/total nitrogen, and highest contents of residual fructose, total mono- and di-saccharides and dry matter as well as high lactic acid/acetic acid and lactic acid content. FJLB addition had the lowest pH value and the highest lactic acid content, the most intensive lactic acid fermentation occurring in FJLB treated silages. This resulted in the faster accumulation of lactic acid and faster pH reduction. Sorbic acid and FJLB additions depressed clostridia or other undesirable bacterial fermentation, thus this decreased the water-soluble carbohydrates loss and saved the fermentable substrate for lactic acid fermentation.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1699-1704
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• 2007

This experiment was carried out to evaluate the effects of adding encapsulated-glucose, glucose, sorbic acid or prefermented juice of epiphytic lactic acid bacteria (FJLB) on the fermentation quality and residual mono- and disaccharide composition of Italian ryegrass (Lolium multiflorum Lam) silages. The additive treatments were as follows: (1) control (no addition), (2) encapsulated-glucose addition at 0.5% for glucose, (3) glucose addition at 1%, (4) sorbic acid addition at 0.1%, (5) FJLB addition at a theoretical application rate of $2.67{\times}10^5$ CFU (colony forming unit) $g^{-1}$, on a fresh weight basis of Italian ryegrass. Although control and encapsulated-glucose treatments had higher contents of butyric acid (33.45, 21.50 g $kg^{-1}$ DM) and ammonia-N/Total nitrogen (114.91, 87.01 g $kg^{-1}$) as compared with the other treated silages, the fermentation in all silages was clearly dominated by lactic acid. This was well indicated by the low pH (4.38-3.59), and high lactic acid/acetic acid (4.39-22.97) and lactic acid content (46.85-121.76 g $kg^{-1}$ DM). Encapsulated-0.5% glucose and glucose addition increased lactic acid/acetic acid, and significantly (p<0.05) decreased ammonia-N/total nitrogen, and the contents of butyric acid and total volatile fatty acids (VFAs) as compared with the control. However, there were higher butyric acid and lower residual mono-and di-saccharides on the two treatments as compared with sorbic acid and FJLB addition, and their utilization efficiency of water soluble carbohydrates (WSC) was lower than that of both sorbic acid and FJLB additions. Sorbic acid addition showed the lowest content of ethanol and ammonia-N/total nitrogen, and the highest content of residual fructose and total mono-and disaccharides as well as the higher lactic acid/acetic acid value. Sorbic acid addition decreased the loss of mono-and disaccharides, and inhibited the activity of clostridial and other undesirable bacteria, and greatly increased the utilization efficiency of fermentable substrates by epiphytic LAB. FJLB addition had the lowest pH value and the highest lactic acid content among all additive treatments, with the most intensive lactic acid fermentation occurring in FJLB treated silage. This resulted in the faster accumulation of lactic acid and faster pH reduction. Sorbic acid and FJLB addition depressed clostridia or other undesirable bacterial fermentation which decreased the WSC loss and saved the fermentable substrate for lactic acid fermentation.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1273-1278
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• 2005

This study is to evaluate the effect of different levels of ensiling density on the fermentation quality of guineagrass silages during the early stage of ensiling. Guineagrass at the milky ripe stage was chopped and ensiled into a small-scale laboratory silo at two ensiling density levels (high density at 95 g/silo and low density at 75 g/silo). Three silos per level were opened after six ensiling periods (0.5, 1, 1.5, 2, 3 and 7 days of ensiling) and the fermentation qualities were analyzed. Within the initial 1.5 days of ensiling there were not significant (p>0.05) differences in the fermentation qualities between two density levels silages, and an almost constant pH and no or only small amounts of lactic acid, acetic acid and total volatile fatty acids were detected. However, the high density silage significantly (p<0.05) increased the rate and extent of fermentation after 1.5 days of ensiling, which was well reflected in significantly (p<0.05) faster and larger pH decline and lactic acid production at each elapsed time as compared with the low density silage. This resulted in significantly (p<0.05) lower finial pH and significantly (p<0.05) higher lactic acid content at the end of the experiment. Moreover, there was higher AA content relative to LA in both the H-D and L-D silages during the full fermentation course, and resulted in the AA-type silage. There were generally somewhat or significantly (p<0.05) higher acetic acid, volatile fatty acids and ammonia-N/total nitrogen in the high density silage than in the low density silage during the initial 3 days of ensiling. However, there were higher (p>0.05) ammonia-N/total nitrogen and significantly (p<0.05) higher butyric acid content in the low density silage at day 7 of ensiling. The silages of two density levels showed an initial increase in glucose between 0.5 and 1 day for the high density silage and between 1 and 1.5 days for the low density silage, respectively, thereafter showed a large decrease until the end of the experiment. There were not large differences (p>0.05) in ethanol content between the low density and high density silages that showed small amounts within initial 3 days of ensiling. However, the low density silage had a significantly (p<0.05) higher ethanol content than the high density silage at the end of experiment. From the above results it was suggested that the increase in ensiling density was an effective method to improve the fermentation quality, especially for tropical grasses.