• Journal of Plant Biotechnology
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• 제1권2호
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• pp.109-115
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• 1999

A linkage map of Capsicum annuum L. was constructed by random amplified polymorphic DNA (RAPD) markers followed in a backcross population of an intraspecific cross between cultivars HDA210 and Yatsufusa. A total of 420 random primers were tested and 311 polymorphic bands were generated by 158 random primers. Among them, 86 Yatsufusa specific bands generated by 52 primers were examined for mapping. Most bands except three segregated in Mendelian fashion fitting the expected 1:1 ratio. The total length of the map was 533 cM distributed in 15 linkage groups. The map distance between adjacent markers ranged 0 to 32.8 cM, with an average distance of 9.1 cM (63 markers). Some markers were clustered and this may be due to the amplification of a repetitive sequence by the RAPDs. Primer pairs for a sequence characterized amplified region (SCAR) were developed and the segregation scores by the SCAR primers were in accordance with the RAPD data. Two QTL markers for number of axillary shoots and for early flowering were developed. One QTL for early flowering located in the linkage group 3 and explained 61 "io of the phenotypic variation. The other QTL for the number of axillary shoots located in the linkage group 4 explained 55 % of the phenotypic variation.tion.

• Journal of information and communication convergence engineering
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• 제14권1호
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• pp.26-34
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• 2016

The alignment of DNA sequences is one of the important processes in the field of bioinformatics. The Smith-Waterman algorithm (SWA) performs optimally for aligning sequences but is computationally expensive. Field programmable gate array (FPGA) performs the best on parameters such as cost, speed-up, and ease of re-configurability to implement SWA. The performance of FPGA-based SWA is dependent on efficient cell-basic implementation-unit design. In this paper, we present an optimized systolic cell design while avoiding oversimplification, very large-scale integration (VLSI)-level design, and direct mapping of iterative equations such as previous cell designs. The proposed design makes efficient use of hardware resources and provides portability as the proposed design is not based on gate-level details. Our cell design implementing a linear gap penalty resulted in a performance improvement of 32× over a GPP platform and surpassed the hardware utilization of another implementation by a factor of 4.23.

• 미생물학회지
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• 제28권1호
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• pp.27-34
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• 1990

지속성 및 유발성 발한의 두 macrolide-lincosamide-streptogramin B 저항성 인자가 한 Staphylococcus aureus DHI 균주의 염색체 DNA 및 plasmid pDE1(7.4kb)로부터 각각 분리되었다. pDE1상의 유발성 Em 저항성 인자의 염기서열은 이미 보고 된 바 있는 pE194상의 ermC와 동일하였으며 지속성 Em 저항성 인자의 경우는 그 제한효소 인식부위의 mapping 결과로 보아 ermCdb전자에서 유발성 기구에 관여하는 leader peptide 부위가 결여된 인자인 것으로 밝혀졌다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 학술대회 논문집 정보 및 제어부문
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• pp.486-488
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• 2008

300 MHz가 넘는 초고자장 MRI에서는 송신 또는 수신 RF Magnetic Field 의 불균일도가 심해져서 이를 개선하기 위한 많은 방법들이 제안되고 있다. 그 중 가장 대표적인 방법은 $4{\sim}32$ 채널의 Transmit Array의 각 채널에 인가되는 전압과 위상을 변화시켜 RF Magnetic Field의 불균일도를 개선하는 방법이다. 본 논문에서는 Transmit Array 내부에서 머리위치의 변화에 따라 RF Magnetic Field ($B_1$ Field) 의 불균일도가 많이 변화하며 이에 따라 RF 송신용 전압과 위상의 Pattern을 새로 최적화 해야 함을 확인하였다. 또한 RF field Mapping을 하기 위해서 Composite RF Sequence를 사용한 Rapid Sequence의 사용과 채널 전압과 위상을 최적화하기 위해서 일반적인 Iterative 방식보다 간편하고 빠른 Target Method를 제안하였다. Driving 패턴의 최적화는 Complex 행렬식을 사용했으며 RF Magnetic Field ($B_1$ Field) 분포는 FDTD 방식으로 계산하였다.

• BMB Reports
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• 제39권4호
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• pp.464-467
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• 2006

Recently, the nucleotide sequences of entire genomes became available. This information combined with older sequencing data discloses the exact chromosomal location of millions of nucleotide markers stored in the databases at NCBI, EMBO or DDBJ. Despite having resolved the intron/exon structures of all described genes within these genomes with a stroke of a pen, the sequencing data opens up other interesting possibilities. For example, the genomic mapping of the end sequences of the human, murine and rat BAC libraries generated at The Institute for Genomic Research (TIGR), reveals now the entire encompassed sequence of the inserts for more than a million of these clones. Since these clones are individually stored, they are now an invaluable source for experiments which depend on genomic DNA. Isolation of smaller fragments from such clones with standard methods is a time consuming process. We describe here a reliable one-step cloning technique to obtain a DNA fragment with a defined size and sequence from larger genomic clones in less than 48 hours using a standard vector with a multiple cloning site, and common restriction enzymes and equipment. The only prerequisites are the sequences of ends of the insert and of the underlying genome.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.844-848
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• 2002

In more than half of human tumors, the p53 tumor suppressor gene is mutated. Thus, restoration of wild-type p53 activity by repair of mutant RNA could be a potentially promissing approach to cancer treatment. To explore the potential use of RNA repair for cancer therapy, trans-splicing group I ribozymes were developed that could replace mutant p53 RNA with RNA sequence attached to the 3'end of ribozymes. By employing a mapping library of ribozymes, we first determined which regions of the p53 RNA are accessible to ribozymes, and found that the leader sequences upstream of the AUG start codon appeared to be particularly accessible. Next, trans-splicing ribozymes were generated that specifically recognized the sequences around these accessible regions. Subsequently, the ribozymes reacted with and altered the p53 transcripts by transferring a 3'exon tag sequence onto the targeted p53 RNA with high fidelity. Thus, these ribozymes could be utilized to repair mutant p53 in tumors, which would revert the neoplastic phenotype.

• 한국수학교육학회지시리즈D:수학교육연구
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• 제26권4호
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• pp.291-310
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• 2023

In this paper, we propose a theoretical framework for Chinese high school mathematics teachers use new textbooks based on the work of Remillard (1999) and Chau (2014). Based on this framework, a multiple case approach was used to investigate how two high school mathematics teachers from Shanghai use new textbooks. The results suggest that in the curriculum mapping arena, both the novice teacher and the expert teacher often planned to appropriate the unit content, and sometimes planned to add supplemental content. When organizing the unit content, novice teacher always planned to follow the new textbook in sequence, while expert teacher often would follow the new textbook in sequence, but sometimes planned to rearrange the unit content. In the design arena, both the novice teacher and the expert teacher tended to appropriate the introduced tasks and definitions. The novice teacher often planned to appropriate the example problems and exercise problems, while the expert teacher often intended to flexibly use the example problems and exercise problems. In the construction arena, the novice teacher seldom adjusted the planned tasks; in contrast, the expert teacher adjusted the planned tasks more frequently. In the reflection arena, the novice teacher often thought she should improve the mathematics tasks, while the expert teacher almost always thought he needed to improve the mathematics tasks. The framework shown in this paper provides a tool to investigate how mathematics teachers use textbooks.

• Investigative Magnetic Resonance Imaging
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• 제2권1호
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• pp.67-72
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• 1998

자기공명 영상의 의학적 이용에서 T2이완시간의 화소별 영상화(T2 이완 영상)는 병변의 정량적 진단도구로서 제안된 바 있다. NMR의 물리/화학적 이용에서 T2 이완시간의 측정으로서 CPMG(Carr-Pucell-Meiboom-Gill) 펄스열이 가장 효과적으로 인정되고, 쓰이고 있으나, 선형자계를 가하는 MR 영상화 장비에서는 측정된 다른 TE의 영상들을 이용한 T2 이완시간의 영상화 자체에 대한 복잡한 계산을 영상기기에서 수행에 문제가 있고, 일반적으로 최대 4개의 CPMG 에코를 영상화하는 펄스열이 제공되어 있다. 좀더 정확한 T2 이완영상화를 위하여 적어도 8개의 다른 반향시간 TE를 가진 영상들을 필용로 하므로, MR 영상화 장비사에서 제공된 펄스열을 이용하면, 적어도 두번 이상의 영상화를 하여야한다. 이는 TR을 2500msec로 할 때 적어도 15분 정도의 시간이 걸리며, 이 동안 환자의 약간의 움직임, 특히 각 4개 단위의 영상화 사이에 움직임에 의한 임의적 잡음이 계산될 T2 영상에 큰 영향을 미친다. 이 연구에서는 시뮬레이션에 의하여 1, 5, 10% 의 이론적인 임의 잡음을 포함한 영상들을 이용하여 잡음이 T2 영상의 작성에 미치는 영향을 연구하였다. 그리고 4개 에코 펄스열을 이용하여 세 번의 영상화로 12개의 영상을 얻고, 이들로부터 4개, 8개의 다른 TE를 가진 영상들을 선택하여 T2 영상을 계산하였다. 그리고 이 연구에서 개발된 8에코 CPMG펄스열로 한 번에 얻은 8개의 영상을 이용한 T2 이완 영상과 결과를 비교하였다. 잡음이 클수록 실제치보다 T2가 길었고, 8-에코 펄스열은 영상화하는 동안에 환자의 움직임을 줄여서 더 정확한 T2 이완 영상을 만들수 있었다.

• 한국해양바이오학회지
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• 제2권3호
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• pp.168-173
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• 2007

of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics. To analyze the transcriptome of olive flounder, Paralichthys olivaceus, we have conducted EST analysis using cDNA libraries made from muscle of P. olivaceus. Redundant ESTs were assembled into overlapping contigs by using the assembly program ICAtools software. We found that the 221 ESTs were composed of 21 clusters and 35 singletons, suggesting that the overall redundancy of the library was 74.7%. Of the 221 clones, 218 clones (98.6%) were identified as known genes by BLAST searches and 3 clones (1.4%) did not match to any previously described genes. Based on major functions of their encoded proteins, the identified clones were classified into 13 broad categories. Sequence analysis of the ESTs revealed the presence of microsatellite-containing genes which may be valuable for further gene mapping studies. This study contributes to the identification of many EST clones that could be useful for genetics and developmental biology of olive flounder.

• 한국전자파학회논문지
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• 제14권3호
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• pp.276-282
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• 2003

OFDM(Orthogonal Frequency Division Multiplexing)은 고속 data 전송에 효과적인 방식이다. 그러나 많은 부반송파들로 인해 높은 PAPR(Peak-to-Average Power Ratio)이 되어 비선형 왜곡이 발생하게 된다. 기존의 PAPR 저감 기법인 SLM(Selective Mapping) 기법은 동일하게 복사된 정보에 phase sequences를 곱한 다음, 이들 중에서 가장 낮은 PAPR을 갖는 신호를 선택하여 전송하는 방식이다. 이 방식은 선택에 관한 부가 정보를 수신기에 전송해야 data를 정확히 복원할 수 있다. 본 논문에서는 SLM 기법에 부가 정보를 삽입하는 방법과 계산량을 줄이는 적응 SLM 기법을 새롭게 제안한다. 그리고, 세가지 phase sequences를 phase rotation factor로 적용하였을 때의 성능을 비교하였다. 제안된 부가정보 삽입의 적응 SLM 기법을 사용한 PAPR 저감성능은 기존의 SLM 기법과 동일하고, 계산량에서는 1/2 이상 감소되므로 더빠른 OFDM통신이 가능하게 된다. 또한 제시한 부가정 보 삽입방식을 적용하여 만족하는 BER 성능을 얻을 수 있음을 확인하였다.