• Title/Summary/Keyword: Map Size

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Construction of a Bacterial Artificial Chromosome Library Containing Large BamHI Genomic Fragments from Medicago truncatula and Identification of Clones Linked to Hypernodulating Genes

  • Park So-Yeon;Nam Young-Woo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.256-263
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    • 2006
  • In the model legume Medicago truncatula, two mutants, sickle and sunn, exhibit morphologically and genetically distinct hypernodulation phenotypes. However, efforts to isolate the single recessive and single semidominant genes for sickle and sunn, respectively, by map-based cloning have so far been unsuccessful, partly due to the absence of clones that enable walks from linked marker positions. To help resolve these difficulties, a new bacterial artificial chromosome (BAC) library was constructed using BamHI-digested genomic fragments. A total of 23,808 clones were collected from ligation mixtures prepared with double-size-selected high-molecular-weight DNA. The average insert size was 116 kb based on an analysis of 88 randomly selected clones using NotI digestion and pulsed-field gel electrophoresis. About 18.5% of the library clones lacked inserts. The frequency of the BAC clones carrying chloroplast or mitochondrial DNA was 0.98% and 0.03%, respectively. The library represented approximately 4.9 haploid M. truncatula genomes. Hybridization of the BAC clone filters with a $C_{0}t-l$ DNA probe revealed that approximately 37% of the clones likely carried repetitive sequence-enriched DNA. An ordered array of pooled BAC DNA was screened by polymerase chain reactions using 13 sequence-characterized molecular markers that belonged to the eight linkage groups. Except for two markers, one to five positive BAC clones were obtained per marker. Accordingly, the sickle- and sunn-linked BAC clones identified herein will be useful for the isolation of these biotechnologically important genes. The new library will also provide clones that fill the gaps between preexisting BAC contigs, facilitating the physical mapping and genome sequencing of M. truncatula.

A Study on Efficient Noise Filtering of Patent Data Analysis and Level Assessment of Patent Technology which improve reliability (특허 데이터 분석시 효율적인 노이즈 제거와 신뢰도가 향상된 특허 기술수준 평가에 관한 연구)

  • Kang, Hee-Seop;Lee, Seung-Ho
    • Journal of Korea Technology Innovation Society
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    • v.15 no.1
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    • pp.105-128
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    • 2012
  • This paper proposes the technological level assessment which improved reliability and the efficient noise elimination methods in the process of establishing patent map analysis data. In order to eliminate efficiently noise (removed by the manual process in the past), the paper applies the Logical Operator 'AND', makes it a program in excel VBA(Visual Basic Application), and obtains the valid data. For the improved reliability technological level assessment of the patents, the study calculates average number of claims, Patent Family Size(PFS), Cites Per Patent (CPP), Triad Patent Families, Standardization Patent Diversification Index (stdPCPI), and haF-index(Hirsch a Family index). The result which applied noise exclusion work showed less than 10% of acquired patent data ratio and confirmed high reliability. The result that apply proposed technological level assessment index makes sure that balanced technological level assessment which improved reliability by producing synthetic technological level assessment.

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A Study on Interior Simulation based on Real-Room without using AR Platforms (AR 플랫폼을 사용하지 않는 실제 방 기반 인테리어 시뮬레이션 연구)

  • Choi, Gyoo-Seok;Kim, Joon-Geon;Lim, Chang-Muk
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.1
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    • pp.111-120
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    • 2022
  • It is essential to make a purchase decision to make sure that the furniture matches well with other structures in the room. Moreover, in the Untact Marketing situation caused by the COVID-19 crisis, this is becoming an even more impact factor. Accordingly, methods of measuring length using AR(Augmented Reality) are emerging with the advent of AR open sources such as ARCore and ARKit for furniture arrangement interior simulation. Since this existing method using AR generates a Depth Map based on a flat camera image and it also involves complex three-dimensional calculations, limitations are revealed in work that requires the information of accurate room size using a smartphone. In this paper, we propose a method to accurately measure the size of a room using only the accelerometer and gyroscope sensors built in smartphones without using ARCore or ARKit. In addition, as an example of application using the presented technique, a method for applying a pre-designed room interior to each room is presented.

Detection of Individual Trees in Human Settlement Using Airborne LiDAR Data and Deep Learning-Based Urban Green Space Map (항공 라이다와 딥러닝 기반 도시 수목 면적 지도를 이용한 개별 도시 수목 탐지)

  • Yeonsu Lee ;Bokyung Son ;Jungho Im
    • Korean Journal of Remote Sensing
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    • v.39 no.5_4
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    • pp.1145-1153
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    • 2023
  • Urban trees play an important role in absorbing carbon dioxide from the atmosphere, improving air quality, mitigating the urban heat island effect, and providing ecosystem services. To effectively manage and conserve urban trees, accurate spatial information on their location, condition, species, and population is needed. In this study, we propose an algorithm that uses a high-resolution urban tree cover map constructed from deep learning approach to separate trees from the urban land surface and accurately detect tree locations through local maximum filtering. Instead of using a uniform filter size, we improved the tree detection performance by selecting the appropriate filter size according to the tree height in consideration of various urban growth environments. The research output, the location and height of individual trees in human settlement over Suwon, will serve as a basis for sustainable management of urban ecosystems and carbon reduction measures.

A Study on the Effect of Grid Size to Extract Topographical Parameters by DEM (DEM에 의한 지형인자 추출에 따른 격자크기의 영향에 관한 연구)

  • Jeong, In-Ju;Lee, Jung-Min;Kim, Sang-Yong;Lee, Jong-Chool
    • Journal of Korean Society for Geospatial Information Science
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    • v.10 no.2 s.20
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    • pp.67-75
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    • 2002
  • Recently, GIS tend to be studied in water resoruces field. In hydrology analysis, GIS propose way that can develop subjective element of designer objectively. The development project is conducting disaster effect estimation to breed disaster, and cope these disaster beforehand provoking soil erosion and food recently. In this study, receive value of LS factor through DEM data at volume of soil erosion computation by disaster effect estimation and whether it are some relation effect of gradient and change of soil erosion by grid size did comparative analysis. As a result, according as grid size great, gradient became slow and could know that error value of gradient great according as storing scale of digital topographical map grows.

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Stereo matching for large-scale high-resolution satellite images using new tiling technique

  • Hong, An Nguyen;Woo, Dong-Min
    • Journal of IKEEE
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    • v.17 no.4
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    • pp.517-524
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    • 2013
  • Stereo matching has been grabbing the attention of researchers because it plays an important role in computer vision, remote sensing and photogrammetry. Although most methods perform well with small size images, experiments applying them to large-scale data sets under uncontrolled conditions are still lacking. In this paper, we present an empirical study on stereo matching for large-scale high-resolution satellite images. A new method is studied to solve the problem of huge size and memory requirement when dealing with large-scale high resolution satellite images. Integrating the tiling technique with the well-known dynamic programming and coarse-to-fine pyramid scheme as well as using memory wisely, the suggested method can be utilized for huge stereo satellite images. Analyzing 350 points from an image of size of 8192 x 8192, disparity results attain an acceptable accuracy with RMS error of 0.5459. Taking the trade-off between computational aspect and accuracy, our method gives an efficient stereo matching for huge satellite image files.

Determination of Variable Rate Fertilizing Amount in Small Size Fields for Precision Fertilizing (정밀 시비를 위한 소구획 경작지내의 가변적 시비처리량 결정)

  • 조성인;강인성;최상현
    • Journal of Biosystems Engineering
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    • v.25 no.3
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    • pp.241-250
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    • 2000
  • The feasibility of precision fertilizing for small size fields was studied by determining fertilizing amount of nitrogenous and calcareous to a cite specific region. A detailed soil survey at three experimental fields of $672m^2$, $300m^2$ and $140m^2$ revealed a considerable spatial variation of the pH and organic matter(OM) levels. Soil organic matter was measured using Walkley-Black method and soil pH was measured with a pH sensor. Soil sample was obtained by Grid Node Sampling Method. The soil sampling depth was 10∼20 cm from the soil surface. To display soil nutrient variation, a soil map was made using Geographic Information System (GIS) software. In soil mapping, soil data between nodes was interpolated using Inverse Distance Weighting (IDW) method. The variation was about 1∼1.8 in pH value and 1.4∼7% in OM content. Fertilizing Amount of nitrogenous and calcareous was determined by th fertilizing equation which was proposed by National Institute of Agricultural Science and Technology(NIAST). The variation of fertilizing amount was about 3∼11 kg/10a in nitrogenous and 70∼140 kg/10a in calcareous. The results showed a feasibility of precision fertilizing for small size fields.

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Vascular Morphometric Changes During Tumor Growth and Chemotherapy in a Murine Mammary Tumor Model Using OCT Angiography: a Preliminary Study

  • Kim, Hoonsup;Eom, Tae Joong;Kim, Jae Gwan
    • Current Optics and Photonics
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    • v.3 no.1
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    • pp.54-65
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    • 2019
  • To develop a biomarker predicting tumor treatment efficacy is helpful to reduce time, medical expenditure, and efforts in oncology therapy. In clinics, microvessel density using immunohistochemistry has been proposed as an indicator that correlates with both tumor size and metastasis of cancer. In the preclinical study, we hypothesized that vascular morphometrics using optical coherence tomography angiography (OCTA) could be potential indicators to estimate the treatment efficacy of breast cancer. To verify this hypothesis, a 13762-MAT-B-III rat breast tumor was grown in a dorsal skinfold window chamber which was applied to a nude mouse, and the change in vascular morphology was longitudinally monitored during tumor growth and metronomic cyclophosphamide treatment. Based on the daily OCTA maximum intensity projection map, multiple vessel parameters (vessel skeleton density, vessel diameter index, fractal dimension, and lacunarity) were compared with the tumor size in no tumor, treated tumor, and untreated tumor cases. Although each case has only one animal, we found that the vessel skeleton density (VSD), vessel diameter index and fractal dimension (FD) tended to be positively correlated with tumor size while lacunarity showed a partially negative correlation. Moreover, we observed that the changes in the VSD and FD are prior to the morphological change of the tumor. This feasibility study would be helpful in evaluating the tumor vascular response to treatment in preclinical settings.

Linkage Disequilibrium and Effective Population Size in Hanwoo Korean Cattle

  • Lee, S.H.;Cho, Y.M.;Lim, D.;Kim, H.C.;Choi, B.H.;Park, H.S.;Kim, O.H.;Kim, S.;Kim, T.H.;Yoon, D.;Hong, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1660-1665
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    • 2011
  • This study presents a linkage disequilibrium (LD) analysis and effective population size ($N_e$) for the entire Hanwoo Korean cattle genome, which is the first LD map and effective population size estimate ever calculated for this breed. A panel of 4,525 markers was used in the final LD analysis. The pairwise $r^2$ statistic of SNPs up to 50 Mb apart across the genome was estimated. A mean value of $r^2$ = 0.23 was observed in pairwise distances of <25 kb and dropped to 0.1 at 40 to 60 kb, which is similar to the average intermarker distance used in this study. The proportion of SNPs in useful LD ($r^2{\geq}0.25$) was 20% for the distance of 10 and 20 kb between SNPs. Analyses of past effective population size estimates based on direct estimates of recombination rates from SNP data demonstrated that a decline in effective population size to $N_e$ = 98.1 occurred up to three generations ago.

Performance Improvement of the battening Effect of the new Asymmetric Turbo Codes (새로운 비대칭 구조를 갖는 터보부호의 Flattening Effect의 성능향상에 관한 연구)

  • 정대호;정성태;김환용
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.6A
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    • pp.533-539
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    • 2002
  • It is well known the fact that turbo lodes has better performance as the number of iteration and the interleaver size increases in the AWGN channel environment. However, as the number of iteration and the interleaver size are increased, it is required much delay and computation for iterative decoding, and caused the flattening effect phenomenon which is very litter BER performance improvement at the arbitrary SNR. In this paper, We proposed the new asymmetric turbo codes, which consist of parallel concatenated turbo codes that use mixed types of component codes with different not only constraint length but also generate polynomial and analyzed its BER performance for log-MAP decoding algorithm with frame size of 128, 256, 512 and 1024 bits, and coding rate of 1/3. As a results of simulation, proposed asymmetric turbo codes verify that its BER performance is superior to conventional symmetric turbo codes. It can be also observed that the flattening effect phenomenon is very reduced by applying the proposed asymmetric turbo codes. It gains respectively 1.7dB ~2.5dB and 2.0dB~2.5dB SNR improvements in the case of short frame(128, 256) and large frame(512, 1024) size for the BER $10_{-4}$>/TEX> region.