• Progress in Medical Physics
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• v.23 no.1
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• pp.33-41
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• 2012

For applying the quality assurance (QA) of volumetric modulated arc therapy (VMAT) introduced in Eulji Hospital, we classify it into three different QA steps, treatment planning QA, pretreatment delivering QA, and treatment verifying QA. These steps are based on the existing intensity modulated radiation therapy (IMRT) QA that is currently used in our hospital. In each QA step, the evaluated items that are from QA program are configured and documented. In this study, QA program is not only applied to actual patient treatment, but also evaluated to establish a reference of clinical acceptance in pretreatment delivering QA. As a result, the confidence limits (CLs) in the measurements for the high-dose and low-dose regions are similar to the conventional IMRT level, and the clinical acceptance references in our hospital are determined to be 3 to 5% for the high-dose and the low-dose regions, respectively. Due to the characteristics of VMAT, evaluation of the intensity map was carried out using an ArcCheck device that was able to measure the intensity map in all directions, $360^{\circ}$. With a couple of dosimetric devices, the gamma index was evaluated and analyzed. The results were similar to the result of individual intensity maps in IMRT. Mapcheck, which is a 2-dimensional (2D) array device, was used to display the isodose distributions and gave very excellent local CL results. Thus, in our hospital, the acceptance references used in practical clinical application for the intensity maps of $360^{\circ}$ directions and the coronal isodose distributions were determined to be 93% and 95%, respectively. To reduce arbitrary uncertainties and system errors, we had to evaluate the local CLs by using a phantom and to cooperate with multiple organizations to participate in this evaluation. In addition, we had to evaluate the local CLs by dividing them into different sections about the patient treatment points in practical clinics.

• Journal of Radiation Protection and Research
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• v.42 no.1
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• pp.9-15
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• 2017

Background: The purpose of this study is to assign an appropriate density to virtual phantom for 2D diode array detector with different dose calculation algorithms to guarantee the accuracy of patient-specific QA. Materials and Methods: Ten VMAT plans with 6 MV photon beam and ten VMAT plans with 15 MV photon beam were selected retrospectively. The computed tomography (CT) images of MapCHECK2 with MapPHAN were acquired to design the virtual phantom images. For all plans, dose distributions were calculated for the virtual phantoms with four different materials by AAA and AXB algorithms. The four materials were polystyrene, 455 HU, Jursinic phantom, and PVC. Passing rates for several gamma criteria were calculated by comparing the measured dose distribution with calculated dose distributions of four materials. Results and Discussion: For validation of AXB modeling in clinic, the mean percentages of agreement in the cases of dose difference criteria of 1.0% and 2.0% for 6 MV were $97.2%{\pm}2.3%$, and $99.4%{\pm}1.1%$, respectively while those for 15 MV were $98.5%{\pm}0.85%$ and $99.8%{\pm}0.2%$, respectively. In the case of 2%/2 mm, all mean passing rates were more than 96.0% and 97.2% for 6 MV and 15 MV, respectively, regardless of the virtual phantoms of different materials and dose calculation algorithms. The passing rates in all criteria slightly increased for AXB as well as AAA when using 455 HU rather than polystyrene. Conclusion: The virtual phantom which had a 455 HU values showed high passing rates for all gamma criteria. To guarantee the accuracy of patent-specific VMAT QA, each institution should fine-tune the mass density or HU values of this device.

• Journal of Pharmacopuncture
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• v.13 no.3
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• pp.63-71
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• 2010

Objectives: Gallic acid (GA) is the major component of tannin which could be easily founded in various natural materials such as green tea, red tea, grape juice, and Corni Fructus. The purpose of this study is to investigate the effect of Gallic acid (GA) on production of interleukin (IL) in mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS). Methods: Productions of interleukins were measured by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. Firstly, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, it was incubated with the antibody-conjugated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System and concentration of interleukin was determined. Results: The results of the experiment are as follows. 1. GA significantly inhibited the production of IL-3, IL-10, IL-12p40, and IL-17 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). 2. GA significantly inhibited the production of IL-6 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). 3. GA diminished the production of some cytokine such as IL-4, IL-5, and IL-13 in LPS-induced mouse macrophage RAW 264.7 cells. 4. GA did not show the inhibitory effect on the production of IL-$1{\alpha}$ and IL-9 in LPS-induced mouse macrophage RAW 264.7 cells. Conclusions: These results suggest that GA has anti-inflammatory activity related with its inhibitory effects on the production of interleukins such as IL-3, IL-10, IL-12p40, IL-17, and IL-6 in LPS-induced macrophages.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.145-145
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• 2017

Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative genes to foxglove aphid resistance in wild soybean, PI 366121 (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4:8 recombinant inbred lines developed from a cross between susceptible variety, Williams 82 and foxglove aphid resistance wild soybean, PI 366121 were used. The two type of resistance response, antibiosis and antixenosis resistance were evaluated through choice and no-choice test, graded by the degree of total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 29,898 single-nucleotide polymorphism markers utilizing a Axiom(R) 180K soyaSNP array. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 was identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. The newly identified major QTL was consistent with previously reported QTL, Raso2, which showed around 5 times narrow down interval range with 8 candidate genes. Furthermore, total 1,115 soybean varieties including Glycine soja and Glycine max were exposed to germplasm screening, and 31 varieties, which showed significant antibiosis type foxglove aphid resistance were identified. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars and developing novel insecticides.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.4
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• pp.586-591
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• 2010

Chemokine and Growth Factor are major mediumtors of immuno-inflammatory pathway. The purpose of this study is to investigate whether productions of Chemokine and Growth Factor in lipopolysaccharide (LPS)-induced mouse macrophage RAW 264.7 cells are modulated by Gallic acid (GA), which is easily founded in tannin-containing natural materials such as red wine, green tea, grape juice, and Corni Fructus. Productions of Chemokine and Growth Factor were analyzed by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. At first, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, the antibody-conjugated beads were added and incubated for 30 minutes. After incubation, detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Based on fluorescence intensity, concentrations of Chemokine and Growth Factor were determined. The results of the experiment are as follows. GA significantly inhibited the production of interferon-inducible protein (IP)-10, keratinocyte-derived chemokine(KC), and vascular endothelial growth factor (VEGF) in LPS-induced RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). GA significantly inhibited the production of monocyte chemoattractant protein-1(MCP-1) and macrophage-colony stimulating factor(M-CSF) in LPS-induced RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). GA diminished the production of granulocyte macrophage-colony stimulating factor (GM-CSF) in LPS-induced RAW 264.7 cells. But GA did not show the inhibitory effect on the production of leukemia inhibitory factor (LIP) and macrophage inflammatory protein (MIP)-2 in LPS-induced RAW 264.7 cells. These results suggest that GA has the immuno-modulating activity related with its inhibitory effects on the production of IP-10, KC, MCP-1, VEGF, and M-CSF in LPS-induced macrophages.

• Korean Journal of Acupuncture
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• v.27 no.3
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• pp.109-118
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• 2010

Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on chemokine and growth factor production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Methods : Productions of chemokine and growth factor were measured by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on xMAP$^{(R)}$ technology. Firstly, cell culture supernatant was obtained after treatment with LPS (1 ${\mu}g$/mL) and SB for 24 hours. Then, it was incubated with the antibody-conjugated beads for 30 minutes. Detection antibody was then added and incubated for 30 minutes. After incubated for 30 minutes, strepavidin-conjugated phycoerythrin (SAPE) was added. After another 30 minutes incubation, the level of SAPE fluorescence was analyzed in Bio-plex Suspension Array System. Results : The results of the experiment are as follows. 1. SB significantly inhibited the LPS-induced production of vascular endothelial growth factor (VEGF), interferon-inducible protein (IP)-10, and granulocyte-colony stimulating factor (G-CSF) at the concentration of 25, 50, 100, and 200 ${\mu}g$/mL in RAW 264.7 cells (P < 0.05). 2. SB significantly inhibited the LPS-induced production of Eotaxin at the concentration of 25, 100, and 200 ${\mu}g$/mL in RAW 264.7 cells (P < 0.05). 3. SB significantly inhibited the LPS-induced production of MIP-$1\alpha$ at the concentration of 25 and 100 ${\mu}g$/mL in RAW 264.7 cells (P < 0.05). Conclusions : These results suggest that SB has immuno-modulatory property related with its inhibition of VEGF, IP-10, G-CSF, and Eotaxin production in macrophages.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.10
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• pp.5129-5152
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• 2016

Multi-view super-resolution (MVSR) aims to estimate a high-resolution (HR) image from a set of low-resolution (LR) images that are captured from different viewpoints (typically by different cameras). MVSR is usually applied in camera array imaging. Given that MVSR is an ill-posed problem and is typically computationally costly, we super-resolve multi-view LR images of the original scene via image fusion (IF) and blind deblurring (BD). First, we reformulate the MVSR problem into two easier problems: an IF problem and a BD problem. We further solve the IF problem on the premise of calculating the depth map of the desired image ahead, and then solve the BD problem, in which the optimization problems with respect to the desired image and with respect to the unknown blur are efficiently addressed by the alternating direction method of multipliers (ADMM). Our approach bridges the gap between MVSR and BD, taking advantages of existing BD methods to address MVSR. Thus, this approach is appropriate for camera array imaging because the blur kernel is typically unknown in practice. Corresponding experimental results using real and synthetic images demonstrate the effectiveness of the proposed method.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.5
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• pp.2366-2395
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• 2018

Multi-view super-resolution (MVSR) refers to the process of reconstructing a high-resolution (HR) image from a set of low-resolution (LR) images captured from different viewpoints typically by different cameras. These multi-view images are usually obtained by a camera array. In our previous work [1], we super-resolved multi-view LR images via image fusion (IF) and blind deblurring (BD). In this paper, we present a new MVSR method that jointly realizes IF and BD based on an integrated energy function optimization. First, we reformulate the MVSR problem into a multi-channel blind deblurring (MCBD) problem which is easier to be solved than the former. Then the depth map of the desired HR image is calculated. Finally, we solve the MCBD problem, in which the optimization problems with respect to the desired HR image and with respect to the unknown blur are efficiently addressed by the alternating direction method of multipliers (ADMM). Experiments on the Multi-view Image Database of the University of Tsukuba and images captured by our own camera array system demonstrate the effectiveness of the proposed method.

• Geophysics and Geophysical Exploration
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• v.3 no.1
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• pp.1-6
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• 2000

The 1 $km^2$ area studied is located in Sukchun-ri, Hwasung-koon, the southern part of Kyeonggi-do. Even though this site has been known as a contaminated area caused by seawater intrusions, geophysical and geochemical surveys have never been carried out at the site to determine the extent of the seawater contamination and to investigate whether the seawater intrusion is in progress. The purpose of this study is to determine the extent of seawater contamination and a preferred channel of the seawater intrusion using geophysical methods such as DC resistivity surveys with Schlumberger array and a dipole-dipole array. In order to determine whether the seawater intrusion is in progress in the area, DC resistivity monitoring with Schlumberger array was performed. According to the resistivity map obtained from the inversion of the resistivity data measured with Schlumberger array, the study area is divided into two districts as relatively lowly resistive (less than 30 ohm-m) and highly resistive (more than 30 ohm-m) areas. The distribution of the lowly resistive area is consistent with the distribution of the layer composed of clay minerals, and the resistivity of this layer decreases slowly as approaching to the old seashore. Hydrogeological analysis shows that the clay layer within a distance of about 200 m from the seashore has been already contaminated by sea-water and its electric conductivity is 8 times higher than that of the sand layer covered by the clay layer. According to the results of the 2-dimensional DC resistivity surveys with a dipole-dipole array, there are two preferred channels of the seawater intrusion in the site, and both the channels are in the NW-SE direction from the old seashore. The lowly resistive zone in the southern channel extends to a depth of 80 m. The DC resistivity monitoring with Schlumberger array was carried out along the preferred channel which has the low resistivity Bone (fracture zone) that extended to a depth of 80 m. The time series of apparent resistivity, measured at a distance of 260 m from the old coast line, fluctuates with a period of 12 hours. From these observations, it can be concluded that the seawater intrusion caused by tidal action is still in progress along the fractured zone interpreted by the DC resistivity surveys with a dipole-dipole array.

• Journal of Korean Society on Water Environment
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• v.25 no.5
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• pp.735-741
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• 2009

In order to support the basic information for planning and performing the environment management such as Total Maximum Daily Loads (TMDLs), it is highly recommended to understand the spatial distribution of water quality and runoff data in the unit watersheds. Therefore, in the present study, we applied Self-Organizing Map (SOM) to detect the characteristics of spatial distribution of Biological Oxygen Demand (BOD) concentration and runoff data which have been measured in the Yeongsan, Seomjin, and Tamjin River basins. For the purpose, the input dataset for SOM was constructed with the mean, standard deviation, skewness, and kurtosis values of the respective data measured from the stations of 22-subbasins in the rivers. The results showed that the $4{\times}4$ array structure of SOM was selected by the trial and error method and the best performance was revealed when it classified the stations into three clusters according to the basic statistics. The cluster-1 and 2 were classified primarily by the skewness and kurtosis of runoff data and the cluster-3 including the basic statistics of YB_B, YB_C, and YB_D stations was clearly decomposed by the mean value of BOD concentration showing the worst condition of water quality among the three clusters. Consequently, the methodology based on the SOM proposed in the present study can be considered that it is highly applicable to detect the spatial distribution of BOD concentration and runoff data and it can be used effectively for the further utilization using different water quality items as a data analysis tool.