• Fisheries and Aquatic Sciences
• /
• v.13 no.1
• /
• pp.71-78
• /
• 2010

We investigated the effect of Cu exposure on the activities of protective antioxidant enzymes in the gills and digestive glands of the manila clam Ruditapes phillippinarum exposed to subchronic concentrations (0, 20, 40, and $80{\mu}gL^{-1}$) of waterborne Cu. No mortality occurred during the experimental period, and no significant condition index differences were observed in any exposure group compared with the control. No significant differences were observed in the digestive glands and gills of the clams observed during 15 days of exposure, but after 30 days, the SOD activity in the gill showed a significant difference between the $80{\mu}gL^{-1}$ Cu-exposed group and the control. GPx activities in the digestive glands and gills were significantly lower after 30 days of Cu exposure. Gill GR activity in the high-exposure group ($80{\mu}gL^{-1}$) was significantly elevated compared with that in the control group. GST activities in the digestive glands of all groups did not change over 30 days. However, GST activity in the gill at $80{\mu}gL^{-1}$ Cu was significantly higher after 15 and 30 days of exposure. GSH activities in the gill showed patterns similar to those of GST activities during exposure periods. In the digestive glands, GSH activity was higher only at $80{\mu}gL^{-1}$ after 30 days exposure. In digestive glands and gills, the MDA levels of clams exposed to $80{\mu}gL^{-1}$ Cu were significantly higher after 30 days of exposure.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2013.06a
• /
• pp.227-230
• /
• 2013

Recently, IMO STW Committee has released a model course of Bridge Resource Management. The Leadership and Teamwork among non-technical skills related to human factor is included at STCW 2010 Manila Amendments. Until now, the BRM education in Korea has been independently operated by each related educational institution, i.e., Shipping Company, University due to absence of model course. In this research the focus is on the simulation module among items for the BRM program. During the simulation from the BRM training, the trainee's reaction and popular functions on simulator have been investigated and analyzed to develop a simulation module for effective BRM education.

• Journal of Convergence for Information Technology
• /
• v.11 no.1
• /
• pp.128-135
• /
• 2021

The possibility and effectiveness of cryopreservation was determined to assess survival rates and improve stock management of thawed embryos of Manila clam, Ruditapes philippinarum. The ideal freezing rates were designed and tested to allow cryoprotectants to equilibrate across the membrane during freezing. Survival rates ranging from 0 to 64.3% were obtained using a stepwise freezing protocol compared with 82.3% control rates. Embryos of Ruditapes philippinarum were equilibrated in 2 CPAs plus sea water for 10 min at 25℃ and then cooled at -1℃/min from 20℃ to -12℃. Straws containing more than 100 embryos were held at 12℃ for 5 min allowing equilibration after seeding and slowly cooled at 2℃/min. to -35℃ for 30 min for equilibration before quenching in liquid nitrogen. Dimethyl sulfoxide (DMSO) is the best cryoprotectant indicated for embryos of R. philippinarum with a survival rate of 64.3±3.28% in the presence of 2.0 M DMSO.

• Microbiology and Biotechnology Letters
• /
• v.51 no.4
• /
• pp.390-402
• /
• 2023

Salmonella are Gram-negative pathogenic bacteria commonly found in food animals such as poultry and swine and potentially constitute risks and threats to food safety and public health through transmissible virulence and antimicrobial resistance (AMR) genes. Although there are previous studies in the Philippines regarding genotypic and phenotypic AMR in Salmonella, there are very few on virulence and their associations. Hence, this study collected 700 Salmonella isolates from swine samples in abattoirs and wet markets among four districts in Metro Manila and characterized their genotypic virulence and β-lactam AMR profiles. Gene frequency patterns and statistical associations between virulence and bla genes and comparisons based on location types (abattoirs and wet markets) and districts were also determined. High prevalence (>50%) of virulence genes was detected encompassing Salmonella pathogenicity islands (SPIs) 1-5 suggesting their pathogenic potential, but none possessed plasmid-borne virulence genes spvR and spvC. For bla, bla_TEM was detected with high prevalence (>45%) and revealed significant associations to four SPI genes, namely, avrA, hilA, mgtC, and spi4R, which suggest high resistance potential particularly to β-lactam antibiotics and relationships with pathogenicity that remain mechanistically unestablished until now. Lastly, comparisons of location types and districts showed variations in gene prevalence suggesting effects from environmental factors throughout the swine production chain. This study provides vital data on the genotypic virulence and AMR of Salmonella from swine in abattoirs and wet markets that suggest their pathogenicity and resistance potential for policymakers to implement enforced surveillance and regulations for the improvement of the Philippine swine industry.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.3
• /
• pp.303-309
• /
• 1999

Mass mortality of the Manila clam, Ruditapes philippinarum has been reported all along the west and south coast of Korea for the past several years. As a pathogenic agent, Perkinsus sp., an endoparasitic protozoan has been identified in this study and believed to be responsible for the mass mortalities. Prevalence and infection intensity of Perkinsus sp. was investigated from a Manila clam population inhabiting at Komsoe Bay in the west coast where mass mortality of the clam has been reported. A total of 142 Manila clam, 50 oyster, Crassostrea gigas, 10 ark shell, Scapharca broughtonii, and 5 predatory gastropada, Rapana venosa were examined for the presence and the quantity of Perkinsus sp. Ray's fluid thioglycollate medium method (FTM method) with modified Mackin's infection intensity scale and Choi's quantitative method were used in detecting and quantifying the parasite. All individuals of R. philippinarum examined in this study were infected with Perkinsus sp., indicating $100\%$ prevalence while none of the oysters and the gastropods exhibited the parasite. Six to ten individual hypnospores of Perkinsus sp. were counted from the ark shells. The number of hypnospores in the clam tissues varied from 16,667 to 4,091,667, with a mean number of 1,077,628. Average infection intensity according to Mackin's was 2.87, indicating a moderate infection. A negative correlation was observed between the number of Perkinsus sp. in the tissue and the condition index, a ratio tissue wet weight to shell cavity volume. The clam size and the infection intensity in terms of total number of parasites were positively correlated; the bigger clam, the heavier infection. Such high number of Perkinsus sp. counted in the clams could be enough to cause physiological disturbance of clams, such as retarded growth and reproduction. It is also believed that such a high infection leads mortality of the clam via continuous draining of the energy by metabolic activities and reproduction of the parasites. Correlation between the condition index and the infection intensity observed in this study supports this hypothesis.

• Journal of fish pathology
• /
• v.18 no.1
• /
• pp.39-48
• /
• 2005

Pathogenicity of Vibrio tapetis, the causative bacterium of 'brown ring disease (BRD)' was evaluated in Manila clams (Ruditapes philippinarumi by artificially 0.1 $m\ell$ infection of $1.0\times10^5$cells and $1.0\times10^8$ cells at 20 $^{\circ}C$. A PCR assay based on 16S rRNA to detect the bacteria in clam tissues was established. Accumulative mortality of clams infected with $1.0\times10^7$cells and $1.0\times10^4$ cells per an individual of the bacteria was 67.5% and 7.5%, respectively. However, the deposit of brown pigment in the inner shells by accumulation of chonchiolin was not found. The bacteria were not be able to re-isolate from the infected clams by the conventional agar plate method but were easily detected by PCR assay established in this experiment. In clams artificially infected with 10 species of Vibrio, a 414bp for V. tapetis was detected in PCR assay. The specific band in the clams infected with $1.0\times10^4$cells per an individual of V. tapetis was detected only in gills one day after the infection but never be found in any tissues including gills three days after the infection. In the case of clams infected with $1.0\times10^8$cells per an individual of V. tapetis the specific band was detected in gills and intestine one day after the infection, in all tissues three days after the infection, and then in gills and adductor muscle nine days after the infection. The PCR assay was applied to detect V. tapetis in manila clam, surf clam (Mactra veneriformis), oyster (Crassostrea gigas) and Thomas' rapa whelk (Rapana venosa) taken from Taean and Gochang from April to July 2004. The infection rates were detected to 23.1% and 9.4% in the oyster and surf clam, while manila clam and Thomas' rapa whelk were not found.

• The International Journal of Costume Culture
• /
• v.8 no.1
• /
• pp.43-50
• /
• 2005

This research analyzed the anthropometric data for Filipino plus-size women. The study aims to identify key dimensions used for the sizing system that can improve on fit and style concerns/issues for the plus-sizes. This study also develop a reference of body measurements for Filipino plus-size women from the data collected. The data was collected from subjects residing or working in the University of the Philippines and within Metro Manila. The full figure body shapes have been derived from the raw anthropometric data. The pear and the barrel shapes were derived figure types based on the anthropometric data collected. These body types show that weight distribution is on the lower torso for the pear and the upper torso for the barrel.

• Journal of Korean Dental Science
• /
• v.2 no.2
• /
• pp.31-34
• /
• 2009

The survival rate of the OSSTEM GS II Implant 1 year after serving the prosthetic function in 2 domestic and foreign medical institutes was 97.57%; the success rate was 95.7%, and the average alveolar bone resorption was 0.24mm(n=389). In particular, the alveolar bone resorption occurred differently according to the placement location as well as whether or not the patient underwent bone grafting operation, but the implant s length and diameter did not have significant impact on alveolar bone resorption.

• International Journal of Advanced Culture Technology
• /
• v.6 no.2
• /
• pp.86-99
• /
• 2018

This study examined the relationship between the demographic profiles of 260 staff nurses in the SPC Health Care Ministry Hospitals and their Transcultural Self-Efficacy (TSE) level on the Cognitive, Practical and Affective dimensions. To obtain the result, among the nine (9) demographic profile items, three (3) common positive predictors were considered, namely, 1) Educational attainment level; 2) Age; and 3) Willingness to work abroad in all three dimensions. While in the Practical and Affective dimensions, the number of years employed were a common negative predictor and in the Affective dimension, Gender of participants was added as the positive predictor. Therefore, there are some significant demographic characteristics of respondents influence to the transcultural self-efficacy. Further, based on the results of this diagnostic study, the researcher designed the One (1) year Cultural Competence Training Program for staff nurses. For future research, the researcher recommended to conduct a further study that will evaluate the effectiveness of the educational intervention by approaching a longitudinal study form administering the test and retest of TSET.

• Journal of Energy Engineering
• /
• v.27 no.3
• /
• pp.63-67
• /
• 2018

The Democratic People's Republic of Korea, or the DPRK, is one of the poorest country in world facing chronic economic, energy and food security issues. Prolonged improper management of economic and natural resources has led to extreme poverty, malnutrition and critically vulnerability to nature's forces. Presistent deforestation and forest degradation in the DPRK has national and global consequences which has attracted attention from international community, whose offering financial and technical assistance for targeted interventions. Through REDD+ programs, the DPRK has the opportunity to establish its credentials as a responsible nation while improving the quality of life of its population. This study offers an enabling context under which suitable climate change action related to forestry can be identified and implemented in the DPRK.