• Title/Summary/Keyword: Manila

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Development of a PCR Assay for Detection of the Protozoan Parasite Perkinsus (PCR 기법을 이용한 바지락포자충 Perkinsus 진단 기술개발)

  • 박경일;박영미;이제희;최광식
    • Korean Journal of Environmental Biology
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    • v.20 no.1
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    • pp.109-109
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    • 2002
  • Detection of protozoan parasites Perkinsus sp. and P. atlanticus was developed in this study using a specific polymerase chain reaction (PCR) to diagnose the presence of those organisms that causes extensive mortalities of marine shellfishes. The PCR was conducted together with fluid thioglycollate medium (FTM) method and 2 M NaOH lysis method. For the test, Manila clams, Ruditapes philippinarum, were collected from four coastal locations in Korea including Wando Island, Gimnyeong, Sungsan and Sogwipo in Jeju. In addition, trophozites of Perkinsus sp. cultivated in vitro and the granular ark clam, Tegillarca granosa, taken from Gangjin on the south coast of Korea, were used as positive and negative controls, respectively. Expected DNA bands were detected in the samples from Wando Island, Sungsan and the in vitro cultured Perkinsus sp. when the probes specific for the genus Perkinsus and P. atlanticus were used. The samples were also positively diagnosed by the FTM and 2 M NaOH methods. In contrast, the Manila clams from Gimnyeong and Sogwipo, and the granular arks clams from Gangjin showed no detectable signs of infection with the PCR, the FTM method and the 2 M NaOH lysis method. On the other hand, being amplified by p. atlanticus specific primer, it is suggested that the protozoan parasite Perkinsus sp. found in the Korean Manila clam is P. atlanticus. Finally the PCR- based assay developed in the present study can be used in detection of Perkinsus infection and discrimination of Peykinsus species in quarantine stations or laboratories due to the high sensitivity and specificity as well as its rapid detection.

Changes in density and culture conditions of the Manila Clam Ruditapes philippinarum on the West coast of Korea (서해안 바지락 양식장의 서식환경과 서식밀도의 변화)

  • Park, Kwang-Jae;Song, Jae-Hee;Choi, Yoon-Seok;An, Kyoung-Ho
    • The Korean Journal of Malacology
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    • v.29 no.3
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    • pp.207-216
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    • 2013
  • Changes in density of manila clam, Ruditapes philippinarum to environment, catch and recruitment were studied in a few stations (Seonjae, Seongam, Hwangdo and Padori) in the West coast area of Korea from January, 2007 to December, 2009. Water temperature, salinity, dissolved oxygen and pH in the study stations were 0.8 to $31.2^{\circ}C$, 22.1 to 33.7 psu, 5.0 to 12.0 mg/L and 7.39 to 8.99, respectively. The concentrations of dissolved inorganic nitrogen, phosphate and silicate were 0.016 to 1.281 mg/L, 0.004 to 0.093 mg/L and 0.016 to 1.617 mg/L, respectively. Chlorophyll-a ranged from 0.2 to 12.1 ${\mu}g/L$, respectively. Substrata were mainly composed of muddy sand and very poorly sorted in Padori, muddy sand and well sorted in Seonjae and Hwangdo, gravelly muddy sand and poorly sorted in Seongam. Density was high in Seonjae and Seongam, but low in Hwangdo and Padori. In clam culture station, in which spat was naturally produced without sowing seedlings, the living density was decreased by increasing of death and a catch of shellfish, and recruitment was changed. Also, Density affected condition factor and shape of clam. Condition factor was the highest in Hwangdo, in which temperature in the winter and chlorophyll-a were high, and was the lowest in Padori. In the shape of clam, the shape in Seongam was a elongated form, but in Padori was a stunted form.

Mass Balance Using the LOICZ Model in Gomso and Geunso Bays (LOICZ 모델을 이용한 곰소만, 근소만 갯벌어장 물질수지 산정)

  • Choi, Yong-Hyeon;Cho, Yoon-Sik;Choi, Yoon-Seok;Jeon, Seung Ryul
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.7
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    • pp.869-877
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    • 2017
  • Recently, Gomso Bay saw a decrease in the production of manila clam, which accounted for 17.8 % of production across the whole country in 2015, while Geunso Bay saw an increase that raised its contribution to 49.1 %. The tidal flats in these bays are inhabited by various benthos, and material flows in and out according to the tide. It is essential to understand the mass balance of these regions while giving consideration to the characteristics of these tidal flats. In this study, we compared areas where aquaculture farms were located in Gomso Bay with those without from May to August 2015. We divided the region into three sea areas (Sector I, Sector II, and Control), and the mass balance was estimated using the LOICZ model. As a result, the DIP of Sector II in Gomso Bay and another region in Geunso Bay, where a manila clam farm was located, were found to be -207.2 kg/day and 77.2 kg/day. The DIN was -4,996.7 kg/day and 926.6 kg/day. These results suggest that Gomso Bay han a higher density than Geunso Bay, which is thought to be mainly due to the feeding action of organisms. Therefore, in order to maintain a healthy tidal flat ecosystem and to sustain the production of manila clams, sustainable management to reduce the high density of tidal flat farms is necessary.

Comparison of the Seawater-Sediment Environment and Habitat Properties with Variable Mud Shrimp Upogebia major Burrow Hole Density and Its Influence on Recruitment and Settlement in the Cheonsu Bay Tidal Flats (천수만 갯벌, 쏙(Upogebia major) 유입 및 정착 밀도에 따른 해수-퇴적물 환경과 서식지 특성 비교)

  • Jeon, Seung Ryul;Ong Giho;Koo, Jun-Ho;Park, Jong-Woo;Kim, Yu Cheol;Jeung, Hee-Do;Cho, Jae-Kwon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.2
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    • pp.171-182
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    • 2022
  • The habitat degradation caused by large-scale reclamation leads to devastating impacts, such as fine sediment and mud shrimp Upogebia major settlement on Manila clam Ruditapes philippinarum aquaculture in the eastern Cheonsu Bay tidal flats, Republic of Korea. Despite these impacts, there is a lack of studies on the influence of fine sediments on tidal flats that constitute key mud shrimp habitats. This study provides information on the seawater-sediment environment and the influence of dissolved inorganic nitrogen (DIN) fluctuations depending on mud shrimp burrow hole density. Additionally, it discusses countermeasures for Manila clam habitat management. The results show that mean DIN effluxes in areas with a high-density of burrow holes were up to 4 times (0.12 mmol m-2 d-1) higher than those in sites of low-density (0.03 mmol m-2 d-1) within the Saho and Songhak-ri tidal flats. To manage interference within the competition zone of Songhak-ri tidal flat, it is important to utilize the settlements of spawning season in all three dimensions. Consequently, additional studies in other tidal flats are essential and research in zones where mud shrimps and juvenile clams coexist will help to determine the priorities in the efficient management of clam aquaculture.

과학기술잡지 편집에 관하여 I

  • 고명삼
    • 전기의세계
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    • v.24 no.6
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    • pp.12-17
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    • 1975
  • UNESCO는 UN국제과학기술정보시스템과 동남아시아의 과학기술 정보의 지부결성과정에서 과학, 기술잡지의 편집에 관한 일련의 연수과정을 가졌다. 이 과정은 1973년과 1974년의 두번에 걸쳐 Indonesia에서 열렸고 인도네시아, 한국, 말레이시아, 필리핀, 원남, 태국의 대표들이 참석한 지부연수과정이 1974년 5원 Manila에서 개최되었다. 이 글은 이 연수과정에서 고문으로 일하신 서독의 H.Grunewald 박사의 강의노트 중 중요한 것을 번역 발췌한 것으로 본학회회원으로 하여금 현재 세계적으로 통용되고 있는 특허, 논문체계 및 심사 등에 관한 절차를 이해하는데 도움을 주고져 여기에 실리게 되었음을 밝힌다.

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DPO 양성제도의 전략적 도입에 관한 연구

  • Ye, Byeong-Deok
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2014.10a
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    • pp.31-33
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    • 2014
  • 최근 동적위치제어시스템 (Dynamic Positioning System, DPS)을 설치한 선박과 해양구조물이 늘어나면서 이 시스템을 운영하는 DPO (Dynamic Positioning Operator) 자격증을 취득하고자 하는 해기사가 급증하고 있다, 특히 2010년 STCW Manila Amendments에 DPO 자격과 관련된 사항이 Code B에 채택됨으로서 DPO 관련 자격기준이 급변하고 있다. 그러나 아직 우리나라에는 DPO 자격과 관련된 교육훈련제도가 정비되어 있지 않음은 물론 자격증 취득을 위한 교육훈련기관도 전무한 실정이다. 이 논문에서는 DPO 양성제도의 현황을 고찰한 후 이 제도를 효율적으로 도입하기 위한 방안을 제안하였다.

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