• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.475-481
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• 1999

The study of lignin, a major component of secondary cell wall, has been partly focused on its removal from the woody part in the kraft pulping industry. Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.l95) catalyzes the synthesis of cinnamyl alcohols from corresponding cinnamaldehydes. A cDNA clone, MdCADl, encoding putative CAD from apples (Malus domestica Borkh. cv Fuji) was characterized in this study. The clone contains an open reading frame of 325 amino acid residues, which shows a greater than 80％ identity with Eucalyptus CADl. MdCADl mRNA was detectable in vegetative tissues and was strongly expressed in the fruit. The expression pattern of MdCADl mRNA in the fruit peel after light exposure was also examined. The mRNA was rapidly increased until 1 day after light exposure and remained stable thereafter, suggesting that MdCADl is light inducible. The inducibility of the MdCADl gene was examined using several environmental stresses. Mechanical wounding of leaves increased the MdCADl mRNA level and the induction was further increased by salicylic acid. Southern blot hybridization showed that there is either one or a few copies of CAD genes in apples. To our knowledge, it is believed that MdCADl is the first CAD clone expressed predominantly in fruit.

• 자연과학논문집
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• 제6권1호
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• pp.49-54
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• 1993

사과나무에 대한 칼슘용액의 수관살포 시 칼슘염의 종류에 따른 과실 내 칼슘함량증가효과를 구명하고 자 본 연구를 수행하였다. $CaCl_2$, $CaCO_3$, Ca$(CH_3COO)_2$ 및 Ca$(NO_3)_2$ 등의 칼슘염을 수관살포한 결과 "쓰가루" 품종에는 Ca$(NO_3)_2$, $CaCl_2$ 및 $CaCO_3$, 그리고 "후지" 품종에는 $CaCl_2$와 $CaCO_3$가 과실의 칼슘함량증가에 효과적이었다. 수관살포에 의하여 과실로 흡수된 칼슘은 과피와 과피직하의 과육에 국한되었다. Ca$(CH_3COO)_2$를 살포한 과실은 저장 중에 에틸렌 발생량이 감소하는 경향이었다.

• 원예과학기술지
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• 제34권2호
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• pp.257-268
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• 2016

Physiological characteristics of five new apple cultivars from the Korean apple breeding program were evaluated as a function of harvest time and storage after harvest. Internal ethylene concentration (IEC), flesh firmness, soluble solids concentration (SSC), and titratable acidity (TA) were measured in 'Summer Dream', 'Summer King', 'Green Ball', 'Picnic', and 'Hwangok' apples at harvest, during shelf life at $20^{\circ}C$, and one day after cold storage at $0.5^{\circ}C$ in air. IEC increased during shelf life in 'Summer Dream', 'Summer King', and 'Green Ball' but not in 'Picnic' or 'Hwangok', regardless of harvest time. Flesh firmness decreased towards harvest time and decreased gradually with time in cold storage only in the former three cultivars. In turn, IEC increased during cold storage in the first three cultivars but not for the last two cultivars, irrespective of harvest time. Changes in SSC and TA did not consistently relate to harvest time or storage period but TA tended to decrease as IEC increased. Furthermore, IEC was negatively correlated with flesh firmness except in the 'Green Ball' cultivar but the significance level was much greater in 'Summer Dream' and 'Summer King' (p < 0.0001) than in 'Picnic' (p < 0.01) or 'Hwangok' (p < 0.05) cultivars. Flesh firmness was positively correlated with TA in the first three cultivars but not in the last two cultivars. Overall, the results indicate that cultivars for which IEC increased after harvest had reduced flesh firmness and TA after storage.

• 한국농림기상학회지
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• 제4권2호
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• pp.114-118
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• 2002

충주지역에서 표고가 다른 9개 지점(계명산 5지점, 남산 4지점)에서 측정한 기온 관측자료를 분석하여 기온이 표고가 높아짐에 따라 일정한 비율로 변화하는 단열조건에 의한 기온체감율을 계산하고, 표고별 후지 품종의 만개기를 추정하였다. 충주 계명산 남서사면에서 측정한 시간별 기온자료를 표고와의 관계로부터 표고가 100 m 상승함에 따라 기온은 0.89$^{\circ}C$의 일정한 비율로 체감하였다. 남산 북서사면에서 측정한 시간별 기온자료와 표고와의 상관으로부터는 0.74$^{\circ}C$/100 m의 비율로 기온이 체감함을 알 수 있었다. 충주지역에서 일평균 기온 5$^{\circ}C$ 이상인 날의 발육속도(DVR)를 계산하여 추정한 만개기와 실제로 조사된 만개기는 서로 일치하는 경향이었으며 표고별로 추정된 충주지역의 사과 만개기는 해발고도 100 m 상승함에 따라 4~5일 늦어졌는데 고도에 따라 4월 25~5월 10일의 범위이었다.

• 원예과학기술지
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• 제19권3호
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• pp.358-361
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• 2001

경북 안동과 영양 지역 과수원에서 적숙기에 수확한 '후지' 사과 과실을 중량별로 분류한 후, 과중, 종자 수, 종자 중량과의 관계를 분석하고 이들 요인과 과형지수 및 기형지수와의 상관관계를 조사하였다. 과실당 평균 종자수와 무게는 안동과수원의 경우 8.9개, 종자 중량 0.64g이었고 영양 과수원은 6.3개, 0.47g이었다. 두 과수원의 조사자료를 종합하여 분석한 결과, 과중은 종자 중량과 정의 상관관계가 있었던 반면 종자의 수와는 상관관계가 낮은 것으로 분석되었다. 과형지수는 종자 수나 종자 중량과 미미한 상관관계를 보였으나 과중과 정의 상관관계를 보였다. 과실의 기형지수는 종자수가 많고 종자 중량이 큰 안동과수원 과실에서 낮은 경향을 보였으며 종자의 수 또는 종자 중량과 고도로 유의한 부의 상관관계를 보였다.

• 한국토양비료학회지
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• 제33권4호
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• pp.242-246
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• 2000

근적외 분광분석기를 이용하여 과수의 영양진단을 위한 신속한 엽분석 수단으로 활용하고자 본 시험을 수행하였다. 사과 '후지'품종 잎 177점, 포도 '캠벨얼리' 품종 잎130점의 시료를 이용하여 1,100~2.500nm까지 근적외영역의 흡수스펙트럼을 측정하였다. 총질소 분석은 살리실산-황산으로 분해후 켈달 증류하여 측정하였다. 근적외 스펙트럼과 총 질소함량과의 상관분석을 이용, 검량식을 개발하였고 화학분석한 결과에 대한 예측식을 구하였다. 사과는 중회귀계수 0.965, 검량식의 표준오차 0.086, 포도는 중회귀계수 0.926, 검량식의 표준오차 0.152이었다. 개발된 검량식을 이용하여 예측식을 작성한 결과는 사과의 경우 0.360, 포도 0.210의 결과를 얻었다. 지역간, 년차간 발생되는 변이가 보정된다면 신속한 엽분석 수단이 될 것으로 판단된다.

• Journal of Applied Biological Chemistry
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• 제43권3호
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• pp.170-175
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• 2000

A pot-lysimeter experiment was conducted with 3-year-old 'Tsugaru' apple (Malus domestica Borkh) trees to examine the changes in oxygen diffusion rate (ODR) with lateral flow velocity of water through soil. The influence of lateral water flow velocity on water relations and elemental content in leaf, and sap temperature distribution patterns of the xylem of trees were also determined. Trees were grown under four soil water regimes: (1) fast laterally flowing (FWT, $2.50{\times}10^{-4}cm\;s^{-1}$), (2) slow laterally flowing (SWT, $0.25{\times}10^{-4}cm\;s^{-1}$), and (3) stagnant water table (WLT) at 60-cm, and (4) drip-irrigation at -40 kPa of soil matric potential as a control. The rate of $O_2$ diffusion converged near $2{\times}10^{-3}g\;m^{-2}\;min^{-1}$ for FWT and control soils, but decreased below $1{\times}10^{-3}g\;m^{-2}\;min^{-1}$ 40 days after treatment (DAT) for WLT soils. For SWT soils, however, the ODR at 15 cm below the soil surface was similar to that of control, but at 45 cm below the soil surface, ODR was similar to that of the WLT treatment. Leaf water potential of FWT and SWT plants was similar to that of control plants, but the values for SWT plants declined by 98 DAT. Leaf water potential of WLT plants decreased from -1.86 MPa (9 DAT) to -2.41 MPa (59 DAT) and finally down to -2.70 MPa. The sap temperature measured at 1100-hr was lowest at top and highest at bottom for FWT and control plants, but this pattern of SWT and WLT plants was disturbed from 29 DAT. However, for SWT plants, such thermal disturbance of sap temperature disappeared from 63 DAT.

• 한국농림기상학회지
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• 제11권4호
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• pp.127-134
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• 2009

본 실험은 '후지'/M.9 재배체계에 있어 최근 문제시되는 일소증상 발생에 미치는 한여름의 기온 요인을 구명하기 위하여 실시되었다. 일소증상은 최고기온이 $31^{\circ}C$이상인 누적일수 및 하루 누적 총광량이 많을수록 발생이 증가하였다. 상대적으로 개방된 수관을 형성한 '후지'/M.9은 '후지'/M.26보다 과실이 광에 노출되기 쉬워 일소증상 피해가 더 컸다. '후지'/M.9 사과나무 중에서는 과대지 길이가 짧은 나무에서 일소증상 피해가 더 컸다. 일소증상 발생은 재배지역 및 재식 열방향에 영향을 받지 않았고, 주로 남쪽과 서쪽에 착과 된 과실에서 일소증상 발생이 많았다. 그 이유는 과실이 직사광선에 노출되어 과실온도가 $40{\sim}45^{\circ}C$를 넘었기 때문으로 판단되었다.

• 원예과학기술지
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• 제31권1호
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• pp.38-49
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• 2013

Prohexadione-calcium (Pro-Ca), ethephon and transient water stress were evaluated in a factorial design, as potential inhibitors of early-season shoot growth of high density orchard management of apple (Malus domestica Borkh.) trees. In the experiment, water stress was imposed to one-half of the 7-year-old 'Golden Delicious'/M.9 apple trees in each of 5 blocks, by stopping irrigation for 3 weeks between 35 and 56 days after full bloom (AFB). Within each whole unit, the following Pro-Ca and ethephon treatments were randomly allocated at $2{\times}2$ factorial: a) 0 or 250 $mg{\cdot}L^{-1}$ a.i. Pro-Ca applied at 28 days AFB and b) 0 or 300 $mg{\cdot}L^{-1}$ a.i. ethephon applied twice (35 and 71 days AFB). All trees were hand thinned to king flowers prior to treatments. Vegetative shoot growth was markedly reduced by Pro-Ca, with its effect being obvious within 14 days after application, while ethephon and water stress treatments were less effective. Pro-Ca had no effect on fruit set and yield but slightly increased fruit size. Ethephon substantially reduced the fruit size and yield but had no effect on fruit set. Water stress reduced fruit set, fruit size and yield. With regard to fruit quality, Pro-Ca did not influence fruit shape, flesh firmness and soluble solids contents (SSC) but slightly reduced titratable acidity. Ethephon had no effect on fruit shape but increased firmness, SSC and acidity, while water stress did not influence these fruit quality attributes. Dry weight of dormant spur buds was reduced by both Pro-Ca and water stress, while increased by ethephon. The larger dormant buds led to the larger spur flowers at the tight cluster stage the following spring. Return flowering was promoted only by ethephon, especially on previous season's shoots. There were no significant interactions between Pro-Ca and ethephon or water stress on most variables observed in this study.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.23-23
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• 2021

Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.