• Journal of Plant Biotechnology
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• v.50
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• pp.176-182
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• 2023

Plant regeneration protocols for adventitious shoot organogenesis from apple (Malus domestica 'Fuji') leaf explants were developed in the present study. The effects of dark incubation periods in the early stages of culture, pre-treatment methods, the number of explants per culture container, the type of culture containers, and the orientation of the explants on culture media were evaluated to determine the optimal shoot regeneration conditions for 'Fuji' apple leaf explants. Light incubation of explants produced minimal response. However, dark incubation of explants for 4 weeks during the initial culture period enhanced shoot regeneration frequency. Comparing the number of explants per container, a higher percentage of shoot regeneration was obtained with nine explants per container compared with four explants per container. Pre-treatment, before culture, by dipping explants in a liquid regeneration medium containing 40 g/L of sorbitol for 2 hours produced the highest shoot formation rate, and the time of shoot formation was accelerated. The percentage of shoot regeneration and number of shoots per regenerating explant reached a maximum of 87.5% and 4.7, respectively. The regenerated shoots were elongated and rooted on a rooting medium of 1/4 MS with 0.2 mg/L IBA. The plantlets were successfully acclimatized, and the regenerated plants produced normal phenotypes.

• Applied Biological Chemistry
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• v.20 no.1
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• pp.66-80
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• 1977

This research was carried as a part of the basic study, in which the aptitude of theKorean oak varieties as barrels for aging apple fine spirits was investigated, and thefollowing results were obtained. 1. Following was the result of the chemical analysis of the fruits which are now mass-produced and can be used as a substitute for raw materials for wine production. Apple (Malus pumila Miller var. domestica Schneider) : Total sugar. total acid, volatile acid and pectin of Jonathan (Hong-og) were 13.95%, 0.46%, 0.012%, 0.20% respectively. Total sugar, total acid, volatile acid and pectin of Ralls (Koog-kwang) were 13.35%, 0.43%, 0.011%, 0.45% respectively. 2. Because of low yield of apple juice due to cellulose, pectin, hemicellulose which are present besides sugars, acids in apples, the apple juice were treated with xylanase of Aspergillus niger SUAFM-430, cellulase and pectinase of Aspergillus niger SUAFM-6. This treatment increased the yield of apple juice. And the apple juice was sterilized by adding potassium metabisulfite $(K_2S_20_5)$ and Saccharomyces cerevisae var. ellipsoideus Rasse Johannisberg II (SUAFM-1018) as a cultivation yeast, which has a strong fermentation power was used to ferment. The yield of apple wine based on raw material was 86-87%. The amount of ethanol, extract and methanol obtained from Jonathan and Ralls were 13.5%, 5.4%, 0.04-0.05% respectively. 3. Wines were distilled for two times by the pot still method to make fine spirits. The yield of fine spirits from apple wine mash was 86.6%, and the pH of fine spirits from Jonathan and Ralls were 4.1, 4.2 respectively. 4. The oak chips made of inner part or outer part of 24 Korean oak varieties were used to select the barrel for aging fine spirits. Two oak chips (one oak chip: $1{\times}1{\times}5cm$) of the inner part or of the outer part of each oak variety were dipped into 300 ml of fine spirits, which was bottled in 640ml beer bottle, and followed aging. The colors, flavors and tastes of the fine spirits were checked during 6 months. A. As a criterion for the first screening of oak barrels for aging fine spirits, the rate five of color extraction was determined. The oak chips showed good results in their order as follows and the best 5 varieties were selected. Gal-cham: Quercus aliena Blume (Inner part), Gul-cham: Quercus variabilis Blume (Outer part), Gal-chain: Quercus aliena Blume (Outer part), Jol-cham: Quercus serrata Thumb (Inner and Outer part). Sin-gal-cham: Quercus mongolica Fisher (Outer and Inner part) Sang-su-ri: Quercus acutissima Carruthers (Outer and Inner part) B. To find out the influence of aging temperature on aging, apple fine spirits were aged by dipping each oak chip at room temperature $(24-25^{\circ}C)$) and $45^{\circ}C$. Aging at $45^{\circ}C$ gave the best result followed aging at $30^{\circ}C$ and then at room temperature. C. Apple fine spirits was aged for six months by dipping oak chips in Erlenmeyer flasks and was irradiated with U.V light. The U.V irradiation enhanced the aging effect by nearly two times, compared with the aging without U.V irradiation. D. In aging apple fine spirits by dipping two oak chips, it was observed that the extent of the extraction of most components of oak chips were strongly dependent upon the pH of fine spirits. E. Oak chips of five selected oak varieties and a Limousin white oak from France as a control were used. Each apple fine spitits was dipped by two oak chips, and was aged at room temperature $(24-25^{\circ}C)$, $30^{\circ}C$, $45^{\circ}C$, and with the U.V irradiation at room temperature shaking every week. After six months of aging, the panel test of these aged fine spirits (Young Brandy) showed the following result. Young brandy of apples aged at $45^{\circ}C$ by dipping oak chips of Gal-chain was almost as the fine spirits which were aged at room temperature by dipping Limousin white oak chips from France. Young brandy of with U.V. irradiation at room temperature which were aged by dipping oak chips of Gal-chain was a little worse than that from the fine spirits aged at room temperature by dipping Limousin white oak chips from France. And so, Korean oak varieties are thought to be able to be used for aging every apple fine spirit which was here investigated.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.3
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• pp.130-144
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• 2013

In this study, we examined the effect of high temperature of urban area on the physiological response of apple tree including the photosynthesis, shoot growth, and fruit quality of 'Fuji'/M.9 apple trees planted at Daegu urban area (DUA) and Gunwi rural area (GRA) for 2 years (2009-2010). During the apple growing season (April-October), the average air temperature of DUA was about $3.0^{\circ}C$ higher than that of GRA and the total rainfall of DUA was 130 mm more than that of GRA. During fruit enlargement stage (June-August), the number of days that recorded daily mean temperature of over $30^{\circ}C$ were ten on DUA in 2010, but there was no day when such temperature was experienced in 2009. Average air temperature of DUA during the maturation stage (September-October) was $19.8^{\circ}C$, which was $4.0^{\circ}C$ higher than that of GRA. The higher temperature of over $30^{\circ}C$ during fruit enlargement stage decreased the photosynthetic rate, shoot growth, fruit weight, and soluble solid content of 'Fuji'/M.9 apple tree. The moderate temperature of about $20^{\circ}C$ during maturation stage increased the photosynthetic rate and soluble solid content of 'Fuji'/M.9 apple tree, but decreased fruit red color. In regional comparison with GRA, photosynthetic rate of DUA was changed from lower before rainy season to higher after rainy season. Fruit weight was higher in DUA than that of GRA. However, fruit weight between DUA and GRA did not show the difference when accumulated days that recorded daily maximum temperature over $35^{\circ}C$ of DUA was increased. Compared to the GRA, soluble solid content of DUA was higher, but fruit red color of DUA was less. These results indicate that the poor red coloring is the most problematic in 'Fuji'/M.9 apple tree by global warming and urbanization.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.155-165
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• 2015

Well-feathered (over 10 feathers) 'Fuji'/M.9 apple trees were planted at $3.0{\times}1.0m$ and trained to slender spindle with 2.5 m height or to tall spindle with 3.5 m height, and the vegetative growth, productivity, and fruit quality of two training systems were compared for 8 years. The canopy volume of the tall spindle trees surpassed that of the slender spindle trees 4 years after planting and was 25% larger than that of the slender spindle trees 5 years after planting. The accumulated yield over 8 years for the tall spindle system was 14% higher than that of the slender spindle system. Alternate bearing and incidence of marssonina blotch were observed in both treatments after 5 years of planting. There was often vegetative imbalance in the trees however, the degree of yield loss and vegetative imbalance of the tall spindle trees was lower than those of the slender spindle trees. Soluble solid content and fruit red color of the tall spindle trees were higher than that of the slender spindle trees in 5 year after planting, resulting from increased light penetration in the canopy due to even distribution of lateral branches and from fruit bearing in different height locations of the trees. In conclusion, increasing the tree height to about 3.5 m using slender spindle 'Fuji'/M.9 apple trees planted with over 333 trees per 10a led to better light penetration, yield and fruit quality compared to a conventional wide training system with the slender spindle.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.1
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• pp.1-8
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• 2013

This study was conducted to compare the local climate conditions and growth periods for the apple (Malus domestica 'Borkh') orchards at different altitudes (330, 500, and 670 m) in Jangsu-Gun, Korea. Observation data for the growth period show that the monthly mean air temperatures at the 'Hongro' apple orchard sites decrease with height at the rate of 1.0 to $3.0^{\circ}C$/100 m. The monthly minimum temperatures in April (blooming period for 'Hongro' apple) were 4.3, 2.9, and $0.4^{\circ}C$ at 330, 500, and 670 m, respectively. The monthly mean temperatures in September (i.e., the coloration and maturation period) were 20.6, 18.7, and $14.5^{\circ}C$, respectively. The annual precipitation range varied from 1,234 to 1,439 mm, which tended to increase with height. The heavy rainfall occurred in summer (June to August) and amounted to 827-933 mm. No significant differences in the duration of sunshine were observed amongst the orchards at three different altitudes. The earliest bud break was observed at the 330 m altitude (18 March 2009), which was 4 and 11 days earlier in comparison to those at 500 and 670 m, respectively. The time of full bloom at 330 m was 12 days ahead of that at 670 m. The optimal maturation of fruit (based on skin redness > 80%) was observed between 7 and 10 September at 330 m, 15 and 18 September at 500 m, and 21 and 23 September at 670 m.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.178-183
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• 2014

This study aimed to evaluate the fruit quality characteristics and incidence of flesh browning in response to air storage at $0{\pm}1^{\circ}C$ a fter controlled atmosphere s torage (CA condition: $O_2$ $2.5{\pm}0.5$%, $CO_2$ $1.5{\pm}0.5$%) at $0{\pm}1^{\circ}C$ in 'Fuji' apples (Malus domestica Borkh.). The storage system was performed as followed: air storage for one month, CA storage 4 months + air storage 3 months ( CA 4M + A ir 3M), CA storage 5 m onths + air storage 2 months ( CA 5M + Air 2M) a nd C A storage 6 m onths + air storage 1months (CA 6M + Air 1M), while the control fruits were stored at CA storage for 8 months right after harvest. The incidence of flesh browning ranged from 17.1% to 30.2% during CA storage but not detected under the treatments of CA 4M + Air 3M and CA 5M + Air 2M. The respiration rate was not affected by storage treatments for 6M while the respiration rate was lower in the treatments of CA 4M + Air 3M and CA 5M + Air 2M than the other storage treatments after 7 months. Ethylene production and internal ethylene concentration were lowest in rapid CA storage and increased with a decreasing CA storage duration. Therefore, the results indicate that CA 5M + Air 2M storage treatment should be recommended to maintain the fruit quality and reduce the risk development of flesh browning rather than typical CA storage in 'Fuji' apples.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.378-384
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• 1996

This study was conducted to investigate the influence of root zone temperature on the growth of shoot and root and the mineral contents in leaf of 'Fuji/M26' apple tree. Shoot growth and enlargement of trunk girth increased linearly with increasing root zone temperature. Fresh and dry weight of root reached maximum at $35^{\circ}C$. Water content of root increased with rising root zone temperature. The chlorophyll content of leaves showed insignificant difference with root zone temperature. Leaf water potential was high at $35^{\circ}C$ at 15 day after treatment but 60 day after treatment this was decreased. The nitrogen content of the leaves was not different by root zone temperature whereas the phosphorus content of the leaves was increased at $30^{\circ}C$ in 1993 and at $25^{\circ}C$ in 1994. The potassium content of the leaves reached a maximum at $30^{\circ}C$ in 1993 and $25^{\circ}C$ in 1994. In 1994 the calcium content of the leaves was increased with rising root zone temperature and with lengthening duration of treatment but no such differences were found in 1993. The magnesium content of the leaves was highest at $25^{\circ}C$ in 1993 and at $20^{\circ}C$ in 1994. The nitrogen and potassium content of the roots were increased linearly with root zone temperature in 1993 and 1994 and the magnesium and phosphorus content of the roots were high at $35^{\circ}C$ in 1994 but no such differences were found in the calcium content of the roots.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.501-510
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• 2015

In this study, we investigated the onset and release of endo-dormancy under natural conditions by observing bud break characteristics in 'Fuji' apple trees using water cuttings. Through examinations of bud break rate and days to bud break, we found that the endo-dormancy of 'Fuji' apple tree continues for 70 d from 165 to 255 d after full bloom (DAFB), from late October to early January of the following year. In addition, within 20 d of first bud break, based on a final bud break rate of 60% or more, we able to identify the timing of the changeover from para-dormancy to endo-dormancy, and endo-dormancy to eco-dormancy. Analysis of the chilling requirement during the endo-dormancy period revealed that chilling accumulation up to 255 DAFB to release endo-dormancy amounted to 666 and 517 h based on the CH and Utah models, respectively. Observation of internal changes in the bud during endo-dormancy showed that flower bud differentiation begins from mid-July, and t ime of inflorescence o f the disk f lower is a vailable to f ind. The f lower buds subsequently developed slowly but steadily during endo-dormancy and in the following year in February, the developmental stage of each organ had progressed. Moreover, the flower buds of 'Fuji' apples were mostly healthy during the dormancy period, but some exhibited necrosis of flower primordium, due partial cell damage from the formation of ice crystals rather than a direct effect of the low temperature. Flower buds were formed in both the axillary buds of bourse shoots and terminal buds of spurs, but lower bud differentiation was observed for the terminal buds of spurs at rate of about 65% of total buds, which was directly related to the bud size and shoot diameter.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.542-549
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• 2015

This work was carried out to evaluate the effects of preharvest 1-methylcyclopropene (1-MCP, $Harvista^{TM}$) and postharvest 1-MCP ($SmartFresh^{TM}$) treatments on the fruit quality attributes of cold-stored 'Fuji' ( Malus domestica Borkh.) apples. Fruits were exposed to 0, 95, 125, or $250 mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 3, 2, 1 weeks before harvest (WBH), and treated with 0 or $1{\mu}{\cdot}L^{-1}$ $SmartFresh^{TM}$ at harvest. Fruit was then stored for up to 180 days at $0{\pm}1^{\circ}C$. Fruit fresh weight, Hunter's value a, internal ethylene concentration (IEC), flesh firmness, titratable acidity (TA), and soluble solids content (SSC) in fruit treated with $Harvista^{TM}$ were not different from those of control fruit at harvest. During cold storage, flesh firmness and TA were higher in fruit treated with $250mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 2 and 3 WBH than in control fruit. IEC was 5.5-10.0% lower in fruit treated with $250mg{\cdot}L^{-1}$ $Harvista^{TM}$ at 2 and 3 WBH compared with control fruit as storage duration progressed, while SSC was not affected. Furthermore, flesh firmness, TA, and IEC were affected neither by $Harvista^{TM}$ nor $Harvista^{TM}+SmartFresh^{TM}$ treatments, compared with those fruit quality attributes at harvest. The correlation maps indicated that IEC was negatively correlated with firmness and TA, regardless of $Harvista^{TM}$ application levels. In addition, positive correlations between fruit quality attributes were detected in treatments with $250mg{\cdot}L^{-1}$ $Harvista^{TM}+SmartFresh^{TM}$. Therefore, the results suggest that with a single application of $SmartFresh^{TM}$, a higher level of $Harvista^{TM}$ application would help in retention of fruit quality attributes during cold storage.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.437-446
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• 2013

This study was conducted for three years (2007, 2009, and 2010) to investigate the changes in fruit quality during maturation, and the quality and storage ability of fruits harvested at different times of 'Fuji' apple in Daegu region with a high air temperature during the fall season. Changes in apple fruit quality during the maturation period were investigated from 120-135 days to 183-198 days after full bloom. In comparing quality and storage ability of fruits harvested at different times, fruits harvested more than 180 days after full bloom were used. During the maturation period, poor coloring was the problem for 'Fuji' apple in Daegu region by the high air temperature about $20^{\circ}C$. In comparing quality of fruits harvested at different times, the soluble solid contents and hunter a value were increased by the extended harvest time. Fruit weight during harvest was not affected by different harvest time, while the fruit firmness and titratable acidity during harvest were decreased critically when the freezing damage happened. Ethylene production, fruit firmness, and titratable acidity during cold storage for twenty weeks did not differ according to the different harvest time. Soluble solid contents of fruits harvested at 216 days after full bloom in 2009 were similar at the time of harvest and cold storage. For fruits harvested at 201 days after full bloom, soluble solid content during cold storage was higher than during harvest time. However fruit firmness, soluble solid content, and titratable acidity after cold storage of fruit harvested after freezing damage was lower than those of the fruit harvested before freezing damage. The results show that the extended harvest time of 'Fuji' apples about 2-4 weeks from 180-200 days after full bloom in area with above-air temperature during fall season was seemed to be beneficial to enhancing soluble solid contents and fruit red color, but harvesting after the middle of November was dangerous because minimum air temperature began to fall under $-3.0^{\circ}C$.