• Horticultural Science & Technology
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• v.19 no.3
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• pp.358-361
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• 2001

Factors affecting fruit conformation of 'Fuji' apples (Malus domestica Borkh.) were investigated through correlation study of fruit weight, seed numbers, and seed weight in relation to fruit conformation indices. Apples were harvested from two orchards in 'Andong' and 'Youngyang' areas at commercial maturity and grouped by fruit weight. Average number of seeds and seed weight were 8.9 and 0.64 g in 'Andong' orchard, while 6.3 and 0.47 g in 'Youngyang' orchard, respectively. When pooled data from the both orchards were analyzed, correlation of fruit weight with seed weight was significantly positive, whereas that with seed number was not significant. Fruit shape index determined by length/diameter ratio was positively correlated with fruit weight, while relationship of the shape index with seed number or with seed weight was not significant. Asymmetry index tended to be low in apples from the 'Andong' orchard in which higher seed numbers and seed weight were observed. The asymmetry index showed highly negative correlations with seed numbers and with seed weight.

• The Plant Pathology Journal
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• v.19 no.3
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• pp.162-165
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• 2003

Transformation of Fuji apple (Malus domestica 'Fuji') was performed using Agrobacterium tumefaciens harboring a coat protein (CP) gene of Cucumber mosaic virus (CMV). A plasmid DNA containing the virus CP and NPT II genes was introduced into the loaves of apple by th e Agrobacterium - mediated transformation procedure. Regenerated transformants of the apple were obtained by kanamycin resistance conferred by the introduced NPT II gene. PCR analysis showed that 3 out of 20 putatively selected R0 plant lines contain the CMV-CP gene. Nine putative transgenic lines out of 20 lines were investigated with the PCR analysis; 5 regenerants produced a 450 bp DNA band and 3 regenerants showed a 671 bp DNA band for the NPT II and CMV-CP genes, respectively. Southern hybyidization results demonstrate the successful integration of the CMV-CP gene into the genome of the apple. This is the first report on the generation of useful vius resistance source of transgenic apple for molecular breeding program.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.4
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• pp.242-246
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• 2000

The Near Infrared Reflectance Spectroscopy(NIR) was used to evaluate nutritional diagnosis for rapid leaf analysis method, 177 'Fuji' apple and 130 'Campbell Early' grape leaves were measured by Near Infrared reflectance spectra in the NIR region(1,100~2.500nm). Total nitrogen content was measured by kjelldhal distillation, after salycilic acid-sulfuric acid digestion. An empirical equation to predict total nitrogen content from its spectral signature was developed by adapting the Near Infrared Reflectance Spectroscopy analysis(NIRa) technique and the results were apple-0.965(R). 0.086(SEC), grape-0.926(R), 0.152(SEC). Standard Error of Prediction(SEP) of NIRa for predicting the total nitrogen of apple and grape leaves was 0.360 and 0.210, respectively. It was concluded that Near infrared reflectance spectroscopy analysis is promising method for rapid analysis of apple and grape leaves.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.400-408
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• 2012

Effects of initial controlled atmosphere (CA) treatment on quality maintenance of 'Fuji' apples were assessed and compared with 1-methylcyclopropene (1-MCP) treatment and continuous CA storage. Apples were harvested twice at different maturity, treated with 1 ${\mu}L{\cdot}L^{-1}$ 1-MCP and then stored for 8 months at $0^{\circ}C$ under 3 conditions: air, CA for the first month followed by air (initial CA), and continuous CA (full CA). CA storage was performed with 1.5 kPa $O_2$ and < 1.0 kPa ($N_2$ balance). Following long-term storage, export simulation, refrigerated shipment and local distribution, were performed by holding apples at $0^{\circ}C$ for 2 weeks and on the shelf at $20^{\circ}C$ for 7 days. Both the application of 1-MCP and CA storage reduced ethylene production and respiration rates. Initial CA storage was also effective on reducing the metabolism although the effects were not as noticeable as full CA. Full CA storage with or without 1-MCP treatment maintained titratable acidity, flesh firmness, and sensory quality at the acceptable to excellent level even after the export simulation following 8-month storage regardless of harvest maturity. In contrast, effects of initial CA storage were limited to the maintenance of firmness and texture in early-harvested apples. Overall results indicated that harvest maturity is the critical factor for export fruit quality after long-term storage when separate treatment of initial CA storage or 1-MCP treatment is applied as a postharvest program.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.170-175
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• 2000

A pot-lysimeter experiment was conducted with 3-year-old 'Tsugaru' apple (Malus domestica Borkh) trees to examine the changes in oxygen diffusion rate (ODR) with lateral flow velocity of water through soil. The influence of lateral water flow velocity on water relations and elemental content in leaf, and sap temperature distribution patterns of the xylem of trees were also determined. Trees were grown under four soil water regimes: (1) fast laterally flowing (FWT, $2.50{\times}10^{-4}cm\;s^{-1}$), (2) slow laterally flowing (SWT, $0.25{\times}10^{-4}cm\;s^{-1}$), and (3) stagnant water table (WLT) at 60-cm, and (4) drip-irrigation at -40 kPa of soil matric potential as a control. The rate of $O_2$ diffusion converged near $2{\times}10^{-3}g\;m^{-2}\;min^{-1}$ for FWT and control soils, but decreased below $1{\times}10^{-3}g\;m^{-2}\;min^{-1}$ 40 days after treatment (DAT) for WLT soils. For SWT soils, however, the ODR at 15 cm below the soil surface was similar to that of control, but at 45 cm below the soil surface, ODR was similar to that of the WLT treatment. Leaf water potential of FWT and SWT plants was similar to that of control plants, but the values for SWT plants declined by 98 DAT. Leaf water potential of WLT plants decreased from -1.86 MPa (9 DAT) to -2.41 MPa (59 DAT) and finally down to -2.70 MPa. The sap temperature measured at 1100-hr was lowest at top and highest at bottom for FWT and control plants, but this pattern of SWT and WLT plants was disturbed from 29 DAT. However, for SWT plants, such thermal disturbance of sap temperature disappeared from 63 DAT.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.52-52
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• 2018

Apple (Malus domestica) is one of the most economically important fruits in Korea. But virus infection has decreased sustainable production of apple and caused the serious problems such as yield loss and poor fruit quality. Virus or viroid infection including Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV) and Apple scar skin viroid (ASSVd) has been also reported in Korea, furthermore, its damages and economic losses have increased constantly. In our research, we tried to survey virus infection for commercial nursery trees of major apple cultivars, especially dwarfing rootstocks 'M.9' and 'M.26' as well as scions. Trees were collected from 11 locations which have produced a great amount of apple nursery stocks in Korea. Infection degree was investigated in apple cultivars, 'Hongro' and 'Fuji' using RT-PCR method. In the scion of cultivar 'Hongro', infection ratio of ACLSV, ASPV and ASGV were 100%, 81.8% and 100% respectively. In the rootstock of cultivar 'Hongro', infection ratio of ACLSV, ASPV, ASGV and ApMV were 90.9%, 81.8%, 100% and 9.1% respectively. In the scion of cultivar 'Fuji', infection ratio of ACLSV, ASPV and ASGV were 81.8%, 90.9% and 100% respectively. In the rootstock of cultivar 'Fuji', infection ratio of ACLSV, ASPV, ASGV and ApMV were 81.8%, 90.9%, 100% and 9.1% respectively. Infection of ASSVd was not detected in both cultivars. From our results, it was found that most of apple rootstocks and scions had multiple infections by apple viruses which have caused economic damage in fruit production.

• Biomedical Science Letters
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• v.24 no.3
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• pp.196-205
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• 2018

Reactive oxygen species (ROS) generated from metabolic reactions cause oxidative DNA damage, which results in oxidative tissue injury. Therefore, there is an increasing demand in the intake of high antioxidant sources in order to maintain a healthy environment in cells. In this study, we investigated the antioxidant and anti-inflammatory activities of Malus domestica (apple), Pyrus communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage), and Raphanus sativus L. (radish) obtained from the local market. Since these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties, placing particular emphasis on their antioxidant and anti-inflammatory properties. The samples were processed via an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assays, processed samples showed better antioxidant activities when compared to raw samples and processed pear samples exhibited the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. mRNA expression of pro-inflammatory mediators and cytokines (iNOS, COX-2, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6) was assessed using RT-PCR. As expected, processed samples exhibited better iNOS inhibition when compared to their raw forms and processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples, up to 1 mg/mL concentration, did not exhibit cytotoxicity against RAW 264.7 cells as demonstrated by cell viability assays. Altogether, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

• Journal of Life Science
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• v.16 no.4
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• pp.653-658
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• 2006

An apple polygalacturonase-inhibiting protein (PGIP), that specifically inhibited endopolygalacturonase (PG, EC 3.2.1.15) from Botryosphaeria dothidea, was purified from B. dothidea infected apple (Malus domestica cv. Fuji) fruits. The apple PGIP was a mixed-type inhibitor of PG from B. dothidea. Optimal temperature for the maximum enzyme activity was $40^{\circ}C$, and optimum pH of the purified PGIP was pH 5.0. PGIP was stable up to temperature of $60^{\circ}C$ and was completely suppressed after heating at $70^{\circ}C$ for 10 min, PGIP was stable at pH between 4 and 8. Inhibition of PG by PGIP was reduced by $K^+$, $Cu^{2+}$, $Mg^{2+}$, $Ca^{2+}$ and $Zn^{2+}$ metal ion, sodium dodecyl sulfate (SDS) and 1,2-diaminocyclohexane tetra acetate (CDTA).

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.127-134
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• 2009

This study was carried out to investigate the effects of air temperature during midsummer on sunburn occurrence that had some problems recently on 'Fuji'/M.9 planting system in Korea. There was a positive relationship between sunburn incidence and accumulated days with high air temperature over $31^{\circ}C$, or accumulated total quantum per day. 'Fuji'/M.9 apple trees with relatively open canopies, compared to 'Fuji'/M.26, were more susceptible to sunburn injury because the fruit was more exposed to sunlight. In 'Fuji'/M.9 apple trees, those that were poor in vigor or with short bourse shoot length were more susceptible to sunburn injury. Sunburn incidence was affected by neither regions nor row orientations. Sunburn was observed most often on the fruits on the south and west sides of the trees. The reason was due to high skin temperature of the fruits reaching $40{\sim}45^{\circ}C$ under full sunlight.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.38-49
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• 2013

Prohexadione-calcium (Pro-Ca), ethephon and transient water stress were evaluated in a factorial design, as potential inhibitors of early-season shoot growth of high density orchard management of apple (Malus domestica Borkh.) trees. In the experiment, water stress was imposed to one-half of the 7-year-old 'Golden Delicious'/M.9 apple trees in each of 5 blocks, by stopping irrigation for 3 weeks between 35 and 56 days after full bloom (AFB). Within each whole unit, the following Pro-Ca and ethephon treatments were randomly allocated at $2{\times}2$ factorial: a) 0 or 250 $mg{\cdot}L^{-1}$ a.i. Pro-Ca applied at 28 days AFB and b) 0 or 300 $mg{\cdot}L^{-1}$ a.i. ethephon applied twice (35 and 71 days AFB). All trees were hand thinned to king flowers prior to treatments. Vegetative shoot growth was markedly reduced by Pro-Ca, with its effect being obvious within 14 days after application, while ethephon and water stress treatments were less effective. Pro-Ca had no effect on fruit set and yield but slightly increased fruit size. Ethephon substantially reduced the fruit size and yield but had no effect on fruit set. Water stress reduced fruit set, fruit size and yield. With regard to fruit quality, Pro-Ca did not influence fruit shape, flesh firmness and soluble solids contents (SSC) but slightly reduced titratable acidity. Ethephon had no effect on fruit shape but increased firmness, SSC and acidity, while water stress did not influence these fruit quality attributes. Dry weight of dormant spur buds was reduced by both Pro-Ca and water stress, while increased by ethephon. The larger dormant buds led to the larger spur flowers at the tight cluster stage the following spring. Return flowering was promoted only by ethephon, especially on previous season's shoots. There were no significant interactions between Pro-Ca and ethephon or water stress on most variables observed in this study.