• The Korea Journal of Herbology
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• v.28 no.2
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• pp.61-65
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• 2013

Objectives : Samchulgeonbi-tang (shenzhujianpi-tang) has been prescribed as one of traditional herbal medicine for treatment of stomach diseases since ancient time in Korea. Samchulgeonbi-tang extract was fermented by Lactobacillus spp. for improving the effect. However, the toxicity and safety of fermented Samchulgeonbi-tang (FS) extract were not confirmed. Therefore, this study was performed to evaluate the acute toxicity and safety of FS extract. Methods : To evaluate the acute toxicity and safety of FS extract, several doses of FS extract, 0, 500, 1000 and 2000 mg/kg, were orally administered to 20 male and 20 female ICR mice, respectively. After treatment with FS extract, we observed mortality, general toxicity, behavior and change of body weight for the 14 days. After 14 days of oral administration, all mice were sacrificed and hematological parameters were analyzed from blood serum. Results : In present study, the toxic signs such as mortality or abnormal behaviors by FS extract were not observed. There are no significant differences between FS-treated group and control group in body weight, organ weights, and hematological parameters. Conclusions : The remarkable adverse effects by FS extract were not observed in ICR mice. Also, any death was not occurred at all treated FS doses, 500, 1000 and 2000 mg/kg. Therefore, the approximate lethal dose (ALD) of FS extract may be more than 2000 mg/kg.

• YAKHAK HOEJI
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• v.36 no.2
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• pp.93-109
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• 1992

The purpose of this experiment was to investigate both the immunomodulatory effect of evening primrose(EP) oil and the effects of EP oil on immunoregulation by cyclophosphamide in mice. EP oil at doses of 0.1, 0.2 and 0.4 ml/kg were orally administered to ICR male mice once daily for 28 consecutive days. Cyclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5 mg/kg at 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells(S-RBC). Immnune responses were evaluated by humoral and cellular immune responses and non-specific immune response. The results of this study were summarized as follows; (1) The humoral immune responses such as hemagglutination titer(HA), hemolysin titer(HY), Arthus reaction and plaque forming cell(PFC) were significantly enhanced in the low dose EP oil administered groups(0.1 and 0.2 ml/kg). However, in the high dose EP oil administered group(0.4 ml/kg) the responses were significantly lowered. (2) In the case of cellular immune responses, delayed type hypersensitivity reaction(DTH) was significantly decreased in EP oil whereas rosette forming cell(RFC) was remarkably enhanced. (3) Activities of natural killer cells and phagocyte were generally enhanced in EP oil. In addition, serum albumin and globulin were also increased.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.6
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• pp.367-373
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• 2017

This study was performed using histochemical and immunohistochemical methods to investigate the effect of Gami-Shinkiwhan(GS) on reproductive function of male aged mice. 8-weeks-old ICR mice were used as control group, without any treatment, and 15-month-old ICR mice were used as aging elicited group(AE) and Gami-Shinkiwhan treatment group(GS). AE group didn't restrict diets and drinking for 6 months without any treatment. GS was administered 0.56g/kg/day for 6 months. Compared with AE group, the cell division of sertoli cells, spermatids, and spermatogonial cells was increased and the apoptosis of sertoli cells was decreased on GS group. Androgen receptor positive reaction and $17{\beta}$-HSD positive reaction were significantly increased in the GS group compared to AE group. In addition, the DJ-1 positive reaction was significantly increased and the HDAC3 positive response was significantly decreased in the GS group compared with AE group. Based on the above results, GS prevented the apoptosis of sertoli cells in the tubules, and increased the production of sertoli cells, spermatozoa and testosterone. Based on this, it is thought that it improves male reproductive dysfunction caused by late-onset hypogonadism.

• Journal of Food Hygiene and Safety
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• v.8 no.3
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• pp.163-169
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• 1993

The acute toxicity of lAS-Na(Linear Alkylbenzene Sulfonate-Na) and MES (ASME, Alpha-Sulfo fatty acid Methyl Ester), surfactans, was eyaluated in ICR mice for 14 days. Mice aging 6 weeks were administered orally with 0, 1,000, 1,320, 1,780, 2,280, 3,000 mg/kg of lASNa or 0, 1,000, 1,560, 2,450, 3,830, 6,000 mg/kg of MES in saline. The body weight of the treated animals was not significantly different from the controls. The main clinical signs of animals treated with lAS-Na or MES were diarrhea, decreased motor acthity and piloerection. The congestion in small intestine was only gross finding in dead animals treated with two sulfactants. In this study, $LD_{50}$ values of lAS-Na and MES were eyaluated 1,319 mg/kg in male and 1,402 mg/kg in female mice, 2,040 mg/kg in male and 2,548 mg in female mice, respectiyely.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.2
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• pp.451-454
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• 2004

A total of forty male mice (32∼33 g average initial body weight) were used in a 10-d growth study to evaluate the effects of dietary yeast hydrolysate SCP-20 on reproductive function in male mice. Dietary treatments included 1) CON (basal diet) and 2) TRT (CON diet +1.5% yeast hydrolysate SCP-20). Testis weight of male mice fed TRT diets was higher than that of male mice fed CON diet (p<0.05). Also VSL was significantly (p<0.05) increased in male mice fed TRT diet compared to that in male mice fed CON diet. Male mice fed TRT diet showed significant (p<0.05) improvement in VAP (average-path velocity) and LIN (linearity) compared to male mice fed CON diet. Also, velocity of epididymal sperm of male mice fed TRT diets was higher than that of male mice fed CON diet (P<0.05). In conclusion, the results obtained from this trial suggests that the dietary yeast hydrolysate SCP-20 was an effective means of improving sperm velocity in male mice.

• Korean Journal of Pharmacognosy
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• v.37 no.2 s.145
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• pp.85-91
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• 2006

The present study was undertaken to evaluate the effect of evening primrose oil (EPO) on the male sexual functions. EPO (daily 0.5 ml/mouse) was orally intubated for 28 consecutive days to experimental ICR mice, and same vol. of vehicle to control mice. On the 14th and 28th experimental day, the testis weight, number of complete intromissions and mating, serum testosterone and cGMP levels, prostaglandin leveIs of penile corpus cavernosum smooth muscle cells, and NO-productive activity of endothelial cells were determined. The weight of body and testis, the number of complete intromissions during the 3hour period were somewhat increased in EPO treated mice than those of control. The number of sperm-positive females and testosterone level in serum were increased in experimental groups. The serum cGMP level was significantly increased but the NO production of ionomycin-stimulated HUVEC cells was not affected when EPO was added into cultures. These results suggest that oral administration of EPO enhanced the sexual functions of male mice, and EPO could be developed as a tonic improving sexual functions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.1
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• pp.53-58
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• 2014

The object of this study was to evaluate the single dose toxicity of Choweseuncheng-tang (CWS), a polyherbal formula have been traditionally used as prevention or treatment agent for various diseases as Tae-eumin prescription on Korean medicinal theory, Sasang-euihak, in male and female mice. Aqueous extracts of CWS (yield = 11.00%) was administered to female and male mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 14 principle organs were also examined. As results, we could not find any CWS treatment related mortality and clinical signs, changes in the body and organ weights, gross findings and changes in histopathology of principle organs, except for some dose-independent accidental findings. The results obtained in this study suggest that the 50% lethal dose and approximate lethal dose of CWS aqueous extracts in both female and male mice were considered as over 2,000 mg/kg, the limited highest dosage recommended by KFDA Guidelines, and can be safety used in clinics.

• Laboraroty Animal Research
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• v.33 no.2
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• pp.57-67
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• 2017

The inhibitory effects of Asparagus cochinchinensis against inflammatory response induced by lipopolysaccharide (LPS), substance P and phthalic anhydride (PA) treatment were recently reported for some cell lines and animal models. To evaluate the hepatotoxicity and nephrotoxicity of A. cochinchinensis toward the livers and kidneys of ICR mice, alterations in related markers including body weight, organ weight, urine composition, liver pathology and kidney pathology were analyzed in male and female ICR mice after oral administration of 150, 300 and 600 mg/kg body weight/day saponin-enriched extract of A. cochinchinensis (SEAC) for 14 days. The saponin, total flavonoid and total phenol levels were found to be 57.2, 88.5 and 102.1 mg/g in SEAC, respectively, and the scavenging activity of SEAC gradually increased in a dose-dependent manner. Moreover, body and organ weight, clinical phenotypes, urine parameters and mice mortality did not differ between the vehicle and SEAC treated group. Furthermore, no significant alterations were measured in alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and the serum creatinine (Cr) in the SEAC treated group relative to the vehicle treated group. Moreover, the specific pathological features induced by most toxic compounds were not observed upon liver and kidney histological analysis. Overall, the results of the present study suggest that SEAC does not induce any specific toxicity in the livers and kidneys of male and female ICR mice at doses of 600 mg/kg body weight/day.

• Nutritional Sciences
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• v.8 no.4
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• pp.205-211
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• 2005

This study examined the time course effects of conjugated linoleic acid (CLA) on the body weight, adipose depots and lipid profiles of ICR male mice using two different sources of fats in the diet Ninety eight mice weighing 25 to 30 g were divided into four groups: beef tallow (BT) and fish oil (FO), beef tallow with CLA supplementation (BTC), and fish oil with CLA supplementation (FOC) group. Eight to nine mice in each group were fed with the experimental diets for 1, 2 or 4 weeks, respectively. All mice were fed experimental diets containing $12\%$ of total dietary fat (w/w) with or without $0.5\%$ CLA (w/w). CLA supplementation did not affect the body weight The weight of epididymal and visceral fats were significantly lower in BTC compared to those in BT groups during the periods examined (p<0.05), whereas they were significantly lower in FOC than those in FO only at 4 weeks (p<0.05). The levels of triglycerides in the plasma were significantly decreased in the BTC group than in BT group throughout the experimental periods (p<0.05). But, FOC was only effective at 4 weeks as compared to FO. The levels of total cholesterol and HDL-C were significantly increased in the BTC than in BT during the entire period (p<0.05), whereas there were no difference between FO and FOC on the levelsof total cholesterol and HDL-C. The levels of free fatty acids (FFA) were significantly decreased in BTC than in BT at 1 and 4weeks and in FOC only at 4 weeks as compared to FO (p<0.05). Taken these results together, CLA was more effective in the beef tallow diet in lowering the epididymal and visceral fat weights and triglyceride level rather than fish oil diet with CLA. Furthermore, the effect became clearer at 4 weeks than at one week of the experiment.

• Korean Journal of Oriental Medicine
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• v.18 no.2
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• pp.151-157
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• 2012

In this research, the genotoxic effect of Bupleuri Radix (BR), the dried roots of Bupleurum falcatum Linne has been traditionally used as anti-inflammatory agent, was evaluated using the mouse micronucleus test. BR aqueous extract (yield = 16.52%) was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2,000, 1,000 and 500 mg/kg. Cyclophosphamide (CPA) 70 mg/kg was used as a known genotoxic agent in a positive control. The appearance of a micronucleus (MN) in polychromatic erythrocyte (PCE) is used as an index for genotoxic potential, and PCE ratio is used as an index of cytotoxicity. Although significant (p<0.01) increase of the number of PCE with one or more nuclei (MNPCE) was detected in CPA treated groups, no significant increases of MNPCE numbers were observed in all three different dosages of BR extracts treated mice with over 0.30 of the individual polychromatic erythrocyte ratio in all mice used in this study. The results obtained indicated that BR extract shows no genotoxicity effects up to 2,000 mg/kg dosing levels the limit dosage in rodents.