• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.33-33
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• 2023

Potatoes are the world's 4th major food crop after maize, rice, and wheat and also are a staple food for 1.3 billion people. Due to their wide adaptability to various environmental conditions, their yeild capacity, and high commercial value, potatoes have contributed to global food security. Many potato germplasms are commonly preserved as whole plants in fields or in storage to maintain their particular genetic combinations. However, field maintenance is expensive and has the risk of potential losses from diseases, pests, plant ageing and climate change. Over the past four decades, meaningful efforts have been made toward the safe long-term conservation of potatoes through cryopreservation methods such as droplet-vitrification. In this study, we tested 4 Korean potato varieties('Golden Egg', 'Golden Ball', 'Ja-Young' and 'Ha-Ryeong') with the modified potato droplet -vitrification protocol. Potato shoot tips are precultured in a sucrose-enriched medium(0.3 and 0.7M for 7 and 17hrs, respectively) and submitted to a loading step with C4 solution for osmoprotection. The treated explants were dehydrated with Plant Vitrification Solution(PVS)2 which is 80% A3 solution in ice for 30 minutes. Thawing and unloading steps were performed with 0.8M sucrose solution for 30 sec(40℃) followed by 30min(25℃, room temperature). In a potato post-culture medium(MS+0.1 mg·L^-1 GA₃+0.1 mg·L^-1 kinetin), we obtained a survival rates of post-thawed explants ranging 16.1-82.2%. The results suggest that modified and optimized protocols are required dependinig on every cultivar, genetic and ecological types. To achieve higher survival and regeneration rates, each step within the cryoprocedure must be carefully optimized.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2023년도 춘계학술대회
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• pp.51-51
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• 2023

Global warming has significant effects on the growth and development of wheat and can cause a reduction in grain yield and quality. Grain quality is a major factor determining the end-use quality of flour and a reduction in quality can result economic losses. Therefore, it is necessary to study the physiological characteristic of wheat to understand its response to temperature elevation, which can aid in the development of strategies to mitigate the negative effects of high temperature and sustain wheat production. This study investigated the effects of elevated temperature on grain characteristics and quality during the grain filling period of two Korean bread wheat cultivars Baekkang and Jokyoung. These two bread wheat cultivars were subjected to an increasing temperature conditions regime; T0 (control), T1 (T0+1℃), T2 (T0+2℃) and T3 (T0+3℃). The results showed that high temperature, particularly in T3 condition, caused a significant decrease in the number of grains per spike and grain yield compared to the T0 condition. The physical properties, such as grain weight and hardness, as well as chemical properties, such as starch, protein, gluten content and SDSS, which affect the quality of wheat, were changed by high temperature during the grain filling period. The grain weight and hardness increased, while the grain size not affected by high temperature. On the other hand, amylose content decreased, whereas protein, gluten content and SDSS increased in T3 condition. In this study, high temperature within 3℃ of the optimal growth temperature of wheat, quantity properties decreased while quality-related prosperities increased. To better understand the how this affects the grain's morphology and quality, further molecular and physiological studies are necessary.

• 한국어병학회지
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• 제37권1호
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• pp.79-88
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• 2024

Aeromonas spp. 감염은 관상어 산업뿐만 아니라 양식업에서도 상당한 경제적 손실을 초래하는 것으로 보고되고 있다. 한편 2022년 12월부터 2023년 1월 사이에 국내에서 사육중인 금붕어(Crassius auratus)에서 Aeromonas병증을 보이는 어체에 대해서 발병 원인균을 규명하기 위해서 조사 하였다. 병증을 보이는 금붕어의 피부와 내부 장기에서 그람 음성균이 분리되었다. Biochemical test와 16S rRNA gene으로는 Aeromonas 속까지 동정할 수 있었으나 종레벨까지는 구분하기 어려웠다. 이에 가장 상동성이 높았던 A.veronii를 대상으로 species-specific PCR을 수행했고 명확히 종 수준으로 동정할 수 있었다. 아울러서 장독소 유전자, 감염 실험 및 항생제 내성 분석을 수행하였다. 금붕어에 대한 균주의 생체 내 병원성 시험 결과, 실험 주사 후 일주일 이내에 감염된 숙주에서 100%의 치사율을 나타냈다. 장독소 유전자인 act 독력 유전자가 검출되었다. 균주의 항균제 감수성 분석 결과 대부분의 항균제에 감수성을 보였으나, Ampicillin, Imipenem, Meropenem 및 Clindamycin 약제에는 내성을 보였다.

• 한국방재안전학회논문집
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• 제9권2호
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• pp.63-75
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• 2016

안전한 수돗물 공급을 위해 정수처리장부터 최종 단계인 수도꼭지까지 일정 수준 이상의 잔류염소농도가 유지되어야 한다. 하지만 국내 문헌에 따르면 상수공급의 전체 과정 중에 30-60%의 잔류염소가 소실되고, 이에 대한 주요 원인으로 정수처리 과정에서 염소 사용량 감소 추세, 급수배관 내에서 염소분해 손실, 여름철의 높은 온도에 의한 잔류염소 분해 속도 증가, 급수배관의 노후화에 따른 잔류염소 손실, 저수조 내 저장 시 잔류염소 감소 발생 등이 파악되었다. 이러한 이유로 저수조를 거치는 급수 방식의 경우 최종 수도꼭지의 잔류염소 농도가 기준치보다 낮아질 개연성이 높고, 용량과 체류시간을 단순히 고려하는 기존의 저수조 설계 방식으로 인해서 수돗물 공급의 안전성에 대한 우려가 존재한다. 이의 개선 방안 도출을 위해서 본 연구에서는 저수조 내 잔류염소 감소에 관여하는 주요 기작들인 수체 내 잔류염소 분해, 벽체 표면 흡착, 그리고 증발에 의한 물질전달을 수학적으로 묘사하는 공식들과 계수 값들을 문헌을 통해서 획득하고, 일반적 저수조 조건에서 모델 시뮬레이션을 수행하였다. 그 결과 저수조에 유입되는 수돗물 내 유기물 농도, 수돗물이 저수조에 유입되는 수리학적조건(난류 정도), 그리고 저수조 벽체 표면 재질의 흡착능 등이 저수조 내 잔류염소 감소에 주요 영향 인자들임을 알 수 있었다. 본 연구에서 획득된 결과들은 잔류염소 감소를 최소화하여 안전한 수돗물 공급을 가능하게 하는 새로운 저수조 설계기법이나 기술 개발에 유용하게 활용될 것이다.

• 한국응용곤충학회지
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• 제22권2호
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• pp.52-66
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• 1983

The brown planthopper, N. lugens (Stal), has become a serious pest of rice in tropical Asia during the last decade. At high pest density, its feeding damage causes 'hopperburn' or complete wilting and drying of the rice plant. It also transmits grassy and ragged stunt virus diseases. The estimated losses caused by the pest in tropical Asia exceed $US\$300$ millions. While cultivation of resistant rice varieties has proved to be highly effective against the pest, their long-term stability is threatened because of the evolution of prolific biotypes which can destroy these varieties. At present, identification of biotypes is based principally on the differential reactions of host rice varieties to the pest and on host-mediated behavioral and physiological responses of the pest. Recent findings of morphological differences in adult rostrum, legs, and antennae, body parts that possess receptors for host plant location and discrimination, and cytological differences in N. lugens populations maintained as stock cultures strongly complement other biotype studies. So far, three N. lugens biotypes have been identified in the Philippines. Biotype I can survive on and damage varieties that do not carry and genes for resistance, while Biotype 2 survives on resistant varieties carrying Bph 1 gene and Biotype 3 on varieties carrying gene bph 2. However, none of these biotypes can survive on varieties with genes Bph 3 or bph 4. Several varieties which are resistant in the Philippines are susceptible in India and Sri Lanka as the South Asian biotypes of N. lugens are more virulent than Southeast Asian biotypes. To monitor the pest biotypes in different geographical regions and to identify new sources of resistance, an International Brown Planthopper Nursery has been established in many cooperating countries. The evolution of biotypes is an exceedingly complex process which is governed by the interactions of genetic and biological factors of the pest populations and the genetic makeup of the cultivated varieties. While the strategy for sequential release of varieties with major resistance genes has been fairly successful so far, the monegenic resistance of these varieties makes them vulnerable to the development of the pest biotypes. Therefore, present breeding endeavors envisage utilizing both major and minor resistance genes for effective control of the pest.

• 유통과학연구
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• 제14권5호
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• pp.115-123
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• 2016

Purpose - Nowadays the competition between companies has been intensified in the aviation industry. It is hard to maintain successful market share in challenging managerial environment. Not long ago, a Korean major aviation company had faced social condemnation cause of managerial staff's immoral behaviors. That company suffers great losses in company brand value in terms of authenticity as an aviation company. This research tried to show the effect of brand authenticity in the aviation industry. First of all, this research tried to define the dimensions of the brand authenticities based on the former researches. This research suggested the airline brand authenticities as three kinds of dimensions. The dimensions of authenticities consist of performance aspects, symbolic aspects and moral aspects. And this research also tried to show the relationships between brand authenticities and consumers attitudes. Research design, data and methodology - The empirical research design is based on the experiments with six types of advertisement prototypes. The advertisement prototypes were based on three types of authenticities' characteristics. The prototypes were made of core statements about each authenticity. And the advertisement prototypes also were based on the aviation company types. The types of aviation companies could be divided into FSC(full service carrier)and LCC(low cost carrier). So the whole experiments were performed with six kinds of advertisement prototypes(3 brand authenticities X 2 aviation company types). The age of participants were from 20s to 40s. The proportion of participants' demographics are as follow. Age proportion is 50% of 20s and 50% of 30s and 40s. Gender proportion is 46% males and 54% females. The experiments performed through mobile devices. Advertisement prototypes were exposed to the participants through their mobile devices, and they answered the questionnaires. All the process of experiments were performed by a professional research firm to maintain the quality of data. Results - This research suggested some important outcomes as follow. First, brand authenticity had an important role to make a positive consumer attitude on the aviation company. All the three types advertisement of authenticities had a positive impact on the consumer attitude for the aviation company. Second, the three types of brand authenticities in the performance aspects, symbolic aspects, and moral aspects had a major impact on the consumers attitudes. The performance authenticity had the biggest effect on the consumer attitudes. Third, the types of aviation companies like FSC and LCC had a different correlation with types of authenticities. All the types of authenticities affected on the consumers attitudes in the FSC case. The symbolic authenticity had the biggest effect in the FSC case. But the performance authenticity showed the most striking effect in the LCC case. Conclusion - From this research, we can get a conclusion. The brand authenticity of aviation company should be managed carefully to maintain a positive brand image and consumers attitudes. And airline brand authenticities can be consist of three type dimensions. All the types of authenticities affects on the consumers attitudes positively. The symbolic authenticity affects more in the FSC case, and the performance authenticity influences more in the LCC case.

• 디지털융복합연구
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• 제18권2호
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• pp.197-204
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• 2020

본 논문은 전국과 서울, 부산, 대구, 광주, 대전을 대상으로 아파트 전세가율의 동조화 현상과 변동률을 분석하여 향후 방향성을 찾아보는데 목적이 있다. 이를 위해 국민은행 주택통계에서 2000년 1월부터 2019년 11월까지 총 239개 월간자료를 이용하였다. 상관관계분석에서 전국은 서울, 부산, 인천, 대구 순서로 높은 상관계수를 보여 주었다. 서울은 각 도시와 큰 특징 없이 0.3대의 동일한 수치로 보인 반면 부산과 대전, 대구의 3개 도시는 서로 연관성이 높은 관계로 나타났다. 회귀분석결과, 전국과 주요 5개 도시는 모두 양(+)의 수치를 보여 모두 동일한 방향으로 움직이고 있지만 부산과 서울은 상대적으로 크게 반응하는 것으로 나타났다. 충격반응에서 전국은 서울의 변동에 의해 가장 큰 충격을 받고 있으며, 대구는 부산과 대전에 의해 상대적으로 큰 충격을 받아온 것으로 나타났다. 서울의 전세가율이 최근 3년 동안 가장 강하게 감소하고 있어 갭 투자로 인한 손실이 발생하지 않도록 주의가 필요한 시점이다. 차후 연구를 통해 전세가율과 매매가와의 관계를 살펴본다면 한국 아파트시장을 매우 잘 이해할 수 있을 것이다.

• 한국수정란이식학회지
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• 제6권2호
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• pp.1-17
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• 1991

It is widely recognized that the embryonic or fetal loss after breeding is common in the cattle and that it is an important factor affecting reproductive efficiency. The causes of this loss have been subject of extensive researches and the results indicate that the embryonic mortality may he primary factor responsible for low pregnancy rates in non-embryo transfer bovine populations as well as embryo transfer programs. However, it's causes are still not clearly understood. The embryonic mortality or pregnancy rate has been influenced by various embryonic and maternal effects related to genetic and environmental factors. The timing and extent of embryonic mortality vanes greatly according to authors and estimating methods, because it is difficult to make direct measurements. The major important factors that may influence the embryonic losses or pregnancy rates after embryo transfer can be summeirized. 1.When an embryo is transferred to unmated recipients, the contralateral transfer to corpus luteum results in a lower survival rate than ipsilateral deposition. When the embryos are transferred for the production of twin calves, their survivals and twin pregnancies have quite inconsistent according to the transfer methods either to the unmated-synchronized or already mated recipients and more works are needed to accurrately clarify the previous results. 2.Although embryos can be cultured in vitro some hours without the great declines in pregnancy rates, the rates differ markedly among culture times and media but may be improved by co-transfer systems. 3.Embryo developmental stages and quality grades clearly affect the survival rate following freezing and the pregnancy rate after transfer and the selection of embryos without chromosome abnormalities and of high fertile semen may also be considered to increase the pregnancy rates. 4.Many researches have attempted to relate the plasma progesterone levels to pregnancy rates and others have done either direct progesterone supplementation or luteal stimulation by hCG treatment in order to increase the pregnancy rates. However, these effects on pregnancy rates are inconsistent and also contradictory. 5.The asynchrony between donors or embryos and recipients may he a major cause of embryo death and low pregnancy rate and the sensitivity to uterine asynchyony differs in according to the quality and stages of embryos. 6.The extremes of poor or over nutrition during early pregnancy in the recipients are detrimental to the survival of embryos and the good body condition is required to prevent a reduejion of pregnancy rates. The uterine pathogens in embryonic mortality or fertility have been questioned but the infection of C.pyogenes and Campylobacter fetus is still important pathogens. 7.The heat stress during early pregnancy may reduce conceptus weight and possibly increase the embryonic mortality.

• 한국재난정보학회 논문집
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• 제8권4호
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• pp.362-375
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• 2012

관광호텔은 숙박과 음식점, 운동, 휴양 등의 부대시설을 함께 갖춘 불특정 이용객과 투숙객 이 많은 복합시설로서 화재발생에 따른 인명피해와 재산손실에 매우 취약한 위험이 상존하는 시설이다. 본 연구에서는 관광호텔의 화재발생 현황에 대한 2001년부터 2010년까지 10 년간의 화재통계분석과 대형 호텔화재사례 15건을 분석하였다. 화재발생 장소는 호텔 객실 (33.2%)과 음식점 주방(11.8%)가 주요 발생장소이며 주요 화재원인인 전기화재(40.8%), 담배화재(14.5%), 용접 등 화기작업(9.2%) 등으로 나타났다. 또한 화재사례연구에서는 방화구획설치 및 관리 불량, 가연내장재 사용 등이 연구기간 동안 반복적으로 모든 사례에서 나타나서 주요 화재위험으로 분석되었다. 관광호텔은 이러한 화재위험의 특성에 따라 적합한 방화위험관리의 개발과 현장 운영이 필요하다. 예방점검, 사용자 교육 및 훈련, 투숙객의 화재예방안내 등의 적극적 예방관리가 필요하다. 또한 화재의 연소확대를 차단하기 위한 방화구획의 관리, 스프링클러 등의 자동소화설비를 설치가 필요하며, 가연성 물질의 사용 을 자제하여야 하고, 투숙객 피난 안전에 대한 신속한 조치, 비상절차 등과 관련된 비상대응규정의 운용이 매우 중요하다.

• Journal of Veterinary Science
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• 제21권4호
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• pp.65.1-65.13
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• 2020

Background: Aleutian mink disease virus (AMDV) causes major economic losses in fur-bearing animal production. The control of most AMDV outbreaks is complex due to the difficulties of establishing the source of infection based only on the available on-farm epidemiological data. In this sense, phylogenetic analysis of the strains present in a farm may help elucidate the origin of the infection and improve the control and biosecurity measures. Objectives: This study had the following aims: characterize the AMDV strains from most outbreaks produced at Spanish farms between 2012-2019 at the molecular level, and assess the utility of the combined use of molecular and epidemiological data to track the possible routes of infection. Methods: Thirty-seven strains from 17 farms were partially sequenced for the NS1 and VP2 genes and analyzed phylogenetically with other strains described worldwide. Results: Spanish AMDV strains are clustered in four major clades that generally show a good geographical correlation, confirming that most had been established in Spain a long time ago. The combined study of phylogenetic results and epidemiological information of each farm suggests that most of the AMDV outbreaks since 2012 had been produced by within-farm reservoirs, while a few of them may have been due to the introduction of the virus through international trade. Conclusions: The combination of phylogenetic inference, together with epidemiological data, helps assess the possible origin of AMDV infections in mink farms and improving the control and prevention of this disease.