• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.237-244
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• 2021

Camellia sinensis, Green tea, contains phenolic compounds that act to scavenge reactive oxygen species (ROS), such as catechin, epicatechin, etc. In contrast with the tea leaf, the bioactivity of its fruit and the fruit peels remains still unclear. This study focused on the effects of fruit and fruit peels of C. sinensis (FC and PC) against oxidative DNA damage in NIH/3T3 cells. The scavenging effects of FC and PC on ROS were assessed using 1,1-diphenyl-2-picryl hydrazyl or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radicals. The measurement of ROS in cellular levels was conducted by DCFDA reagent and the protein expression of γ-H2AX, H2AX, cleaved caspase-3, p53, and, p-p53 was analyzed by immunoblotting. The gene expressions of p53 and H2AX were assessed using polymerase chain reaction techniques. The major metabolites of FC and PC were quantitatively measured analyzed and the amounts of phenolic compounds and flavonoids in PC were greater than those in FC. Further, PC suppressed ROS production, which protects the oxidative stress-induced DNA damage through reducing H2AX, p53, and caspase-3 phosphorylation. These results refer that the protective effects of FC and PC are mediated by inhibition of p53 signaling pathways, probably via the bioactivity of phenolic compounds. Thus, FC and PC can serve as a potential antioxidant in DNA damage-associated diseases.

• Steel and Composite Structures
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• 제42권2호
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• pp.151-159
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• 2022

This study discusses a hypothetical method for tracking the propagation damage of Carbon Reinforced Fiber Plastic (CRFP) components underneath vibration fatigue. The High Cycle Fatigue (HCF) behavior of composite materials was generally not as severe as this of admixture alloys. Each fissure initiation in metal alloys may quickly lead to the opposite. The HCF behavior of composite materials is usually an extended state of continuous degradation between resin and fibers. The increase is that any layer-to-layer contact conditions during delamination opening will cause a dynamic complex response, which may be non-linear and dependent on temperature. Usually resulted from major deformations, it could be properly surveyed by a non-contact investigation system. Here, this article discusses the scanning laser application of that vibrometer to track the propagation damage of CRFP components underneath fatigue vibration loading. Thus, the study purpose is to demonstrate that the investigation method can implement systematically a series of hypothetical means and dynamic characteristics. The application of the relaxation method based on numerical simulation in the Artificial Intelligence (AI) Evolved Bat (EB) strategy to reduce the dynamic response is proved by numerical simulation. Thermal imaging cameras are also measurement parts of the chain and provide information in qualitative about the temperature location of the evolution and hot spots of damage.

• Biomolecules & Therapeutics
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• 제17권1호
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• pp.92-97
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• 2009

As nanomaterials might enter into cells and have high reactivity with intracellular structures, it is necessary to assay possible genotoxic risk of them. One of these approaches, we investigated possible genotoxic potential of gold nanoparticle (AuNP) using L5178Y cells. Four different sizes of AuNP (4, 15, 100 or 200 nm) were synthesized and the sizes and structures of AuNP were analyzed using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and stability was analyzed by a UV/Vis. Spectrophotometer. Cytotoxicity was assessed by direct cell counting, and cellular location was detected by dark field microscope at 6, 24 and 48 h after treatment of AuNP. Comet assay was conducted to examine DNA damage and tumor necrosis factor (TNF)-${\alpha}$ mRNA level was assay by real-time reverse transcription polymerase chain reaction. Synthetic AuNP (4, 50, 100 and 200 nm size) had constant characteristics and stability confirmed by TEM, SEM and spectrophotometer for 10 days, respectively. Dark field microscope revealed the location of AuNP in the cytoplasm at 6, 24 and 48 h. Treatment of 4 nm AuNP induced dose and time dependent cytotoxicity, while other sizes of AuNP did not. However, Comet assay represented that treatment of 100 nm and 200 nm AuNP significantly increased DNA damage compared to vehicle control (p <0.01). Treatment of 100 nm and 200 nm AuNP significantly increased TNF-${\alpha}$ mRNA expression compared to vehicle control (p<0.05, p<0.01, respectively). Taken together, AuNP induced DNA damage in L5178Y cell, associated with induction of oxidative stress.

• Journal of Biosystems Engineering
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• 제27권5호
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• pp.391-398
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• 2002

During handling unitized products, they are subjected to a variety of environmental hazards. Shock and vibration hazards are generally considered the most damaging of the environmental hazards on a product and it may encounter while passing through the distribution environment. A major cause of shock damage to products is drops during manual handling. The increasing use of unitization of pallets has been resulted in a reduction of the shock hazards. This has caused an increasing interest in research focused on vibration caused dam age. Damage to the product by the vibration most often occurs when a product or a product component has a natural frequency that falls within the range of the forcing frequencies of the particular mode of transportation being used. Transportation vibration is also a major cause of fruit and vegetable quality loss due to mechanical damage. This study was conducted to determine the vibration characteristics of the corrugated fiberboard bones for packages of pears, and to investigate the degree of vibration injury of the pears in the boxes during the simulated transportation environment. The vibration tests were performed on an electrohydraulic vibration exciter. The input acceleration to exciter was fixed at 0.25 G for a single container resonance test and 0.5 G for the vertical stacked container over the frequency range from 3 to 100 Hz. Function generator (HP-33120A) was connected by wire to the vibration exciter for controlling the input acceleration at a continuous logarithmic sweep rate of 1.0 octave per min. The peak frequency and acceleration on the single box test were 22.02 Hz, 1.5425 G respectively, and these values on the vertical stacked boxes were observed from the bottom box 19.02, 18.14, 16.62 and 15.40 Hz and 2.2987, 3.7654. 5.6087, and 7.9582 G, respectively. The pear in the bottom box had a slightly higher damage level than the fruit packed in the other stacked boxes. It is desirable that the package and transportation system has to be so designed that 15∼20 Hz frequency will not occur during the transportation environment.

• 한국CDE학회논문집
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• 제22권2호
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• pp.141-149
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• 2017

Vulnerability assesses the loss of major performance functions of GCV (Ground Combat Vehicles) when it is hit by enemy's shell. To decide the loss of major functions, it is determined what effects are on the performance of GCV when some components of GCV are failed. M&S (Modeling and Simulation) technology is used to vulnerability assessment. The hydro-pneumatic suspension is used as a sample part. The procedures of vulnerability assessment of the hydro-pneumatic suspension are shown as follows: 1) The components of the suspension are defined, and shot lines are generated evenly around the part. 2) The penetrated components are checked by using the penetration equation. 3) The function model of the suspension is designed by using IDEF0. 4) When the failure of the critical components of the suspension happens, its effect on the function of the suspension can be estimated using DMEA (Damage Mode and Effects Analysis). 5) The diagram of FTA (Fault Tree Analysis) is designed by exploiting DMEA. 6) The damage probability of the suspension is calculated by using FTA and vulnerable area method. In this paper, SLAP (Shot Line Analysis Program) which was developed based on COVART methodology. SLAP calculates the damage probability and visualizes the vulnerable areas of the suspension.

• Computers and Concrete
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• 제32권5호
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• pp.455-464
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• 2023

Seismologists now suggest that the earth has entered an active seismic period; many earthquake-related events are occurring globally. Consequently, numerous casualties, as well as economic losses due to earthquakes, have been reported in recent years. Primarily, significant and colossal damage occurs in reinforced concrete (RC) buildings with masonry infill wall systems, and the construction of these types of structures have increased worldwide. According to a report from the Ministry of Education in the Republic of Korea, many buildings were built with RC frames with masonry infill walls in the Republic of Korea during the 1980s. For years, most structures of this type have been school buildings, and since the Pohang earthquake in 2017, the government of the Republic of Korea has paid close attention to this social event and focused on damage from earthquakes. From a long-term research perspective, damage from structural collapse due to the short column effect has been a major concern, specifically because the RC frame with a masonry infill wall system is the typical form of structure for school buildings. Therefore, the short column effect has recently been a major topic for research. This study compares one RC frame with four different types of RC frames with masonry infill wall systems. Structural damage due to the short column effect is clearly analyzed, as the result of this research is giving in a higher infill wall system produces a greater shear force on the connecting point between the infill wall system and the column. The study is expected to be a useful reference for research on the short column effect in RC frames with masonry infill wall systems.

• Environmental Analysis Health and Toxicology
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• 제21권2호
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• pp.127-138
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• 2006

In urban areas, diesel exhaust particles (DEP) are probably a major component of particulate matters, especially in Korea where drive many diesel vehicles. The aim of this study was to investigate genotoxic effects of DEP using single ceil gel electrophoresis. In order to evaluate the mechanisms of DEP genotoxicity, the rat microsome mediated and DNA repair enzyme treated comet assays together with conventional comet assay were performed. Total diesel particles (DEPT) was collected without site fractionation from diesel engine bus and dichloromethane extract was obtained. The organic extract of DEPT revealed DNA damage itself in NIH/3T3 cells. The level of DNA breaks plus oxidative DNA lesions and microsome mediated DNA damage was assessed by modified single cell gel eletrophoresis. DEPT was able to induce oxidative DNA damage as well as microsome mediated DNA damage. Vitamin C as an model antioxidant reduced DNA damage in endonuclase III treated comet assay. One of flavonoid, galangin as a CYP1A1 inhibitor. reduced DNA damage in the presence of S-9 mixture. $DEP_T$ is the sources of oxidative stress, but antioxidants can significantly reduce oxidative DNA dmage. And $DEP_T$ may contain indirect mutagens which can be inhibited by CYP1A1 inhibitors. The ethanol extracts of the mixed vegetables (BV) or the mixed fruits (BF) were evaluated for their in vitro antigenotoxic effects. BV and BF showed potent Inhibitory effects against DEPT induced DNA damage with oxidative DNA lesions and in the prescence of S-9 mixture. These results indicate that BV and BF could prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P4501A1 in cell culture.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.247-247
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• 2017

Byobusan area of Aomori prefecture in Japan was a marshy sand dune and had developed for agricultural land use with a large-scale sprinkler system. Recently, it becomes an agricultural problem at this area that distinctive damage with browning maculation and fissures frequently occurs in Chinese yam tubers. Acetaldehyde is one of the factor candidates of underground part damage in plants. In this study, incidence rate of the tuber damage, and the morphological character and elemental composition of the damage parts in tubers were investigated with applications of excessive irrigation water or acetaldehyde water solution into the yam field. The incidence rate of the distinctive tuber damage increased as the input amount of irrigation water was increased. At the browning maculation parts of the tubers, many fissures and damages of cork layer were observed under scanning electron microscopy. In addition, the periderm of tubers was significantly thicker in damaged parts than in non-damaged parts. Funguses, bacterium and nematodes were not observed in the damaged part under scanning electron microscopy. The weight ratio of each constituent element in an analyzed area relative to the total weight of major essential elements was measured with energy dispersive X-ray spectrometry. The results showed that the weight ratios of boron, carbon, phosphorus, sulfur and calcium were higher in damaged parts than in non-damaged parts whereas the weight ratios of oxygen and chlorine were lower in damaged parts than in non-damaged parts. It was also shown by this spectrometry that iron, cadmium, lead and zinc were not directly involved in occurrence of the tuber damage. In this study, there was no remarkable difference of tuber appearance between non-acetaldehyde and acetaldehyde application treatments. From the above results, it is shown that the damage would be a physiological disorder induced by the input of a large quantity of water in the sandy field.

• 한국수자원학회논문집
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• 제44권1호
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• pp.1-8
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• 2011

본 연구는 패널자료(15개 시도의 20년간 자료)를 이용하여 홍수피해 비용과 복구비와의 상관관계에 대한 분석을 시도하였다. 패널 분석은 자료의 성격에 따라 고정효과모형 또는 확률효과모형을 사용하지만 본 분석은 두 모형을 함께 추정하였다. 예상대로 모든 변수들은 복구비와 정의 상관관계를 보였지만 사망자수와 이재민 수는 유의하지 않거나 오히려 음의 상관관계를 보이기도 하였다. 그리고 공공시설의 피해가 가장 중요한 인자였다. 무엇보다 중요한 것은 우리나라의 경우 복구비가 피해액이나 투자액에 비해 항상 높다는 것이다. 우리나라와는 반대로 일본의 경우 복구비가 가장 낮고 투자비가 가장 높다는 점을 미루어 볼 때 결국 적절한 투자가 향후 홍수피해는 물론 복구비도 낮출 수 있다는 점을 확인할 수 있었다.

• Journal of the Korean Data and Information Science Society
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• 제19권2호
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• pp.463-473
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• 2008

In this study, we measure damage depth and calculate effective impact speed in case of rear-end collision using real car insurance data. We study the relationship between demage depth and effective impact speed, and present statistical model for these two variables. In our real data study, 3-degree polynomial equation model is better fit to effective impact speed and demage depth than the simple linear model that are estimated in previous other studies. Damage depth is a major factor to see the extent of impact in a car collision, and by using this equation, it is possible to evaluate the severity of driver's injury.