• Journal of Technologic Dentistry
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• v.34 no.4
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• pp.413-421
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• 2012

Purpose: The purpose of this study is to investigate the improvement measure of dental technician practical examination. Methods: A survey was performed on 111 dental technicians who were self-written questionnaire. The results were analyzed by SPSS 12.0. The collected data was analyzed by frequency and chi-square test. Results: Questions most necessary work tools with the articulator was the highest with 55.9%, Electric wax carver(16.2%), heating clear(14.4%), CAD/CAM program(9.0%) was in the order. The need for additional practical test in response to actions included maxillary and mandibular comments occlusal work that you have to wax up the highest rate of 63.8%. Items most in need of improvement was strengthen of clinically oriented(48.0%), separation of written and practical(14.6%), control the degree of difficulty(11.4%) were the next order. Conclusion: Based on the results of this study, the current dental technician did not show major problems in the practical test. However, clinicians should be strengthened mainly. To do this, the use of the articulator is essential.

• Journal of computational fluids engineering
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• v.20 no.4
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• pp.81-92
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• 2015

Construction waste mainly consists of concrete aggregates of various size. Improper handling of concrete waste would be a major environmental problem whereas its recycling would be both economically useful and environmentally friendly. Bigger concrete aggregates are crushed and converted to medium and fine particles to make them recyclable. An apparatus to separate the concrete aggregates by their size is thus needed for their effective recycling. In this work, segregation of concrete particles in air flows from a newly designed rotary separator having three stages of blades is simulated using a commercial software, ANSYS-CFX. Both 2-D and 3-D models with 360, 240 and 180 blades in each stage are considered. Fundamental mechanism of separation of particles(pase) and the effect of design parameters such as particle size, rotor speed, air flow rate etc. on the performance of the separator are investigated. Critical size of particles that can be separated by the developed separator is also presented in this work. Simulation results are overall in good agreement with data predicted from the theoretical model previously reported in the companion paper.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.21-25
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• 2001

Major saponins in ginseng were analysed using reverse phase high performance liquid chromatography with binary mobile phase gradient control system instead of normal phase column. The optimum condition were as following : reverse phase column; ${\mu}{\beta}ondapak\;C_{18}$ column (Waters, $3.9mm{\times}300\;mm,\;5{\mu}m$), methyl cyanaide/water binary mobile phase gradient control system, solvent flow rate; 1.5 ml/min, and UV($203{\mu}m$ ) detector. The complete separation of ginsenoside $Rb_1,\;Rb_2,\;Rc,\;Rd,\;Re,\;Rf\;and\;Rg_1$ was achieved within 55 min. The Regression coefficients of the calibration curves for seven ginsenosides were 0.99.

• Applied Chemistry for Engineering
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• v.8 no.2
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• pp.286-291
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• 1997

Taxol which is recognized as a powerful anticancer reagent was extracted from yew extract and separated by NP-HPLC (Normal-PhaseHigh Performance Liquid Chromatography). The experiments were performed in the isocratic mode with $5{\mu}l$ to $100{\mu}l$ injection volume and 1ml/min mobile phase flow rate. The major mobile phase was hexane and small amounts of ethanol, methanol, 1-propanol and isopropanol were added to change the retention behavior. Prior to a real sample, the artificial mixture of taxol, cephalomannine and 10-deacetyltaxol was tested. They are hard to be separated because of similar chemical structures. The experimental results showed that the proper composition of mobile phase for separating the three components was found 96% hexane and 4% ethanol(vol.%) or 96% hexane, 2% 1-propanol and 2% methanol(vol.%). Compared to the run time of 80 minutes for the binary system, the mixture was separated within 50 minutes with the less amount of mobile phase for the ternary system. Finally, $1{\mu}g$ of taxol was separated from yew tree extracts under the optimum operating conditions.

• Journal of Korean Elementary Science Education
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• v.20 no.1
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• pp.59-73
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• 2001

The National Science Fairs has been held in every year for the promotion of science and technology and scientific civil life. The purpose of this study is to survey the current problems in National Science Fairs by making up a question and to improve them. The study makes up a questionnaires about the existing Science Fairs to the teachers who have submitted their works to the Science Fairs and suggest the ways of improvement. The result of questionnaires is that little availability of reference materials, difficulty of paying proper attention to regular classes because of frequent travels, and absence of experimental devices and experimental rooms are perceived as major obstacles in preparing for the exhibition. Respondents teacher also pointed out some problems with the current organization of the exhibition; non-voluntary selection process for the potential authors, separation of research content from regular teaching materials, unrealistically high standard, low participation rate of teachers, so many exhibited works, lack of professionalism in the screening process. The majority however, agreed that the exhibition event should be continuously done, after remedial measures have been taken about the problems. First, the side of science education is emphasized in a purpose of the science fairs to let activity related to science fair be closely connected with school education. Second, students have to be divided according to grade. Third, research level have to be set not too higher than regular instruction content. Fourth, hand book related to science fairs have to be manufactured and spread. Fifth, data of local science fair have to be arranged. Sixth, judging section have to be subdivided and people related to science education have to be included to a judge. Seventh, excellent works have to be support to participate in ISEF.

• Korean Journal of Pharmacognosy
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• v.45 no.3
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• pp.200-208
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• 2014

In this study, we performed quantification determination of four major components including aurantio-obtusin, emodin, chrysophanol, and physcion in the 70% ethanol extracts of non-processed Cassiae Semen and processed Cassiae Semen using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the 4 constituents used a Gemini $C_{18}$ column kept at $40^{\circ}C$ by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was $10{\mu}L$. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in non-processed Cassiae Semen were 0.07%, 0.02%, 0.25%, and 0.10%, respectively. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in processed Cassiae Semen were 0.04-0.14%, 0.01-0.03%, 0.02-0.42%, and 0.01-0.24%, respectively. Consequently, the optimal processing condition of Cassiae Semen for the improvements of amounts of four anthraquinone compounds was obtained by roasting at $240^{\circ}C$ for 15 min.

• Journal of Ginseng Research
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• v.48 no.4
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• pp.366-372
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• 2024

Background: The biological activity and pharmacological effects of rare ginsenosides have been proven to be superior to those of the major ginsenosides, but they are rarely found in ginseng. Methods: Ginsenoside Rb1 was chemically transformed with the involvement of methanol molecules by a synthesized heterogeneous catalyst 12-HPW@MeSi, which was obtained by the immobilization of 12-phosphotungstic acid on a mesoporous silica framework. High-performance liquid chromatography coupled with mass spectrometry was used to identify the transformation products. Results: A total of 18 transformation products were obtained and identified. Methanol was found to be involved in the formation of 8 products formed by the addition of methanol molecules to the C-24 (25), C-20 (21) or C-20 (22) double bonds of the aglycone. The transformation pathways of ginsenoside Rb1 involved deglycosylation, addition, elimination, cycloaddition, and epimerization reactions. These pathways could be elucidated in terms of the stability of the generated carbenium ion. In addition, 12-HPW@MeSi was able to maintain a 60.5% conversion rate of Rb1 after 5 cycles. Conclusion: Tandem and high-resolution mass spectrometry analysis allowed rapid and accurate identification of the transformation products through the characteristic fragment ions and neutral loss. Rare ginsenosides with methoxyl groups grafted at the C-25 and C-20 positions were obtained for the first time by chemical transformation using the composite catalyst 12-HPW@MeSi, which also enabled cyclic heterogeneous transformation and facile centrifugal separation of ginsenosides. This work provides an efficient and recyclable strategy for the preparation of rare ginsenosides with the involvement of organic molecules.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.12
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• pp.1653-1661
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• 2007

The research aimed to establish the standard extraction procedure for examining brazilin, the major chromophoric substance of Sappanwood, using GC-MS with the ultimate goal of identifying the sappanwood dye in severely faded archaeological textiles. The amount of brazilin represented by the GC abundance was the largest when acetone was used as the extraction medium, followed by methanol. Shaking plate operated at room temperature was more effective than the waterbath shaker which was operated at $30^{\circ}C$. In both cases, the extraction method which incorporated one hour pre-soaking before the 12 hours of actual extraction resulted in a larger amount of brazilin detection than the extraction procedure without the one hour pre-soaking. In case of water extraction, pH 5 resulted in the most effective pH level for the extraction of brazilin, The best GC-MS parameter for detecting brazilin was to set the column temperature initially at $50^{\circ}C$. gradually increase to $210^{\circ}C$ at a $23^{\circ}C/min$ rate, finally increase to $305^{\circ}C$ at $30^{\circ}C/min$ rate, and hold for 14 minutes, and the MSD scan range at $75{\sim}400m/z$.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.3
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• pp.288-294
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• 2005

This study was conducted to evaluate the grit removal efficiency of tornado block-type vortex grit separator. Vortex grit separator was manufactured for this study, and it was characterized by the impeller and tornado block installed in separator. Impeller was installed to increase water velocity in the separator and tornado block was installed to increase the grit lifting efficiency. Pilot study was also conducted in A sewage treatment plant (STP) in Gyeonggi province from November 2003 to May 2004 (64 days). Major findings are as follows. 1. Impeller was proven to increase water velocity in the grit separator, especially in low flow rate. This influence will increase separation ratio of organics from grits, preventing those organics from sedimentation. 2. Sand (with 0.2~0.3mm size) removal efficiency was over 98 % and 96 %, at the flow rate of $500m^3/day$ and $750m^3/day$ under the condition that impeller rotation velocity kept at 15 rpm. Originally that grit separator was designed to have the capacity of $500m^3/day$. $750m^3/day$ was tried to investigate the performance of this type of grit separator under overload condition. Stable grit removal was still available to the extent of 150% of designed capacity. 3. It took less than 3 minutes for the grit separator to completely lift out 3 kgs of 0.2-0.3 mm sized, settled sand at the bottom to 2,060 mm high above water surface. But it showed the tendency to spend a little more time on lifting the grit as the grit size and the vertical height of the lift increased. 4. During experimental duration in A STP, it was found that the average amount of inlet grit was about 981 g/day (160~1,685 g/day) under $500m^3/day$ of operation condition, but it varied so severely during the experimental duration. After classification of discharged grit according to its size, grit with 0.3-0.42 mm size was found as largest part of output.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.5
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• pp.368-372
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• 1984

This paper deals with a rapid method for determination of ATP and its related compounds in fish and shellfish products using high performance liquid chromatography(HPLC). The HPLC used is a HPLC/ALC-224 equiped with UV-spectrophotometer (254 nm) as detector and integrator (Yanagimoto system-1000). The column used is a stainless steel tubing ($30.0\;cm{\times}3.9\;mm\;i.d.$) packed with ${\mu}-Bon-dapak\;C_{18}$. A mixture of $1\%$ triethylamine-phosphoric acid(pH6.5) was used as an eluent and the flow rate of the eluent was controlled at 2 ml/min. For the separation of ATP and its related compounds, a standard mixture of ATP, ADP, AMP, IMP, inosine and hypoxanthine was subjected to HPLC under the above mentioned conditions. Six peaks were obtained with retention times within 20 min, and elution order were hypoxanthine, IMP, inosine, AMP, ADP and ATP. But 5'-IMP and 5'-GMP fractions were not separated by this method. In generally, IMP content in boiled-dried fish and shellfish products purchased from the market was comparatively higher than that of other nucleotides. Especially, boiled-dried big eye herring marked higher value in IMP content than other boiled-dried ones. Hypoxanthine and inosine were major components of ATP-related compounds in dried products and seasoned-dried ones. And IMP content in seasoned-dried products was higher than that of dried ones. This fact is suggested that a part of IMP in seasoned-dried ones was derived from flavoring matter (MSG, 5'-IMP and 5'-GMP) which is added during the seasoning treatment.