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Quantitative Analysis of Anthraquinones in Cassiae Semen by Processing Method  

Seo, Chang-Seob (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Hwang, Seock-Yeon (Department of Biomedical Laboratory Science, College of Natural Science, Daejeon University)
Kim, Byoung-Soo (Department of Physiology, College of Korean Medicine, Daejeon University)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.3, 2014 , pp. 200-208 More about this Journal
Abstract
In this study, we performed quantification determination of four major components including aurantio-obtusin, emodin, chrysophanol, and physcion in the 70% ethanol extracts of non-processed Cassiae Semen and processed Cassiae Semen using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the 4 constituents used a Gemini $C_{18}$ column kept at $40^{\circ}C$ by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was $10{\mu}L$. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in non-processed Cassiae Semen were 0.07%, 0.02%, 0.25%, and 0.10%, respectively. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in processed Cassiae Semen were 0.04-0.14%, 0.01-0.03%, 0.02-0.42%, and 0.01-0.24%, respectively. Consequently, the optimal processing condition of Cassiae Semen for the improvements of amounts of four anthraquinone compounds was obtained by roasting at $240^{\circ}C$ for 15 min.
Keywords
Cassiae Semen; Processing method; Aurantio-obtusin; Emodin; Chrysophanol; Physcion;
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