• Journal of the Korean Society of Tobacco Science
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• v.26 no.2 s.52
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• pp.168-178
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• 2004

Uncertainty of final measurement results considering main uncertainty sources being in nicotine of mainstream smoke was estimated. This study was accomplished by using the ISO 'The Guide to the Expression of Uncertainty in Measurement'. Using the two point re-calibration method, uncertainty for nicotine concentration was calculated considering the uncertainty sources of each step. The concentration and uncertainty of nicotine in mainstream smoke was estimated as $153.95{\pm}17.84\;{\mu}g/mL\;(0.77\pm0.089 mg/cig)$. The expanded uncertainty was $17.84 {\mu}g/mL(\pm0.089 mg/cig).$ The reported expanded uncertainty of the measurement is stated as the standard uncertainty of measurement multiplied by a coverage factor of 2, which for a normal distribution corresponds to a coverage probability of approximately $95\%$ The former expression indicates the conversion concentration into the sample.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.144-151
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• 2006

A measurement uncertainty for analysis of volatile organic compound (benzene) in cigarette mainstream smoke was carried out. In this study one point re-calibration method was used to estimate uncertainty for benzene. The measurement uncertainty was calculated based on the uncertainty sources of each analysis step, quality appraisal sources, drift and repeatability. As a result, the concentration and expanded uncertainty of benzene in cigarette mainstream smoke were measured as $38.08{\pm}4.36{\mu}g/cig$. Relative uncertainty of drift and repeatability obtained were 5% and 3%, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.152-157
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• 2006

A new procedure has been developed for the quantitation of aromatic amines in mainstream cigarette smoke. Two solid-phase extraction (SPE) clean up steps, using a different retention mechanisms, are required to process the samples. The first step used a cation-exchange cartridge, followed by a second step that used a cartridge with a hydrophobic retention character. The aromatic amines eluted from the second SPE cartridge are derivatized with pentafluoropropionic anhydride. This new method have advantages over other reported techniques, being sensitive, robust, and easily automated. The detection limits were ranged from 0.12 ng/mL for 1-aminonaphthalene to 0.16 ng/mL for 3-aminobiphenyl and the recoveries were from 97 to 106%. Compared with other reports for analysis of 2R4F reference cigarette, this method shows a close analytical data and good repeatability.

• Journal of the Korean Society of Tobacco Science
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• v.32 no.1
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• pp.35-40
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• 2010

This study was carried out to enhance the analytical methods of phenolic compounds in mainstream cigarette smoke using high efficiency column and RRLC(Rapid Resolution Liquid Chromatography) system, and to compare these methods. RRLC system offers significantly faster results with higher data quality of phenolic compounds than conventional HPLC, but it is disadvantage that it is expensive. On the other hand, the method using monolithic column offers faster results by the use of conventional HPLC system without new equipment introduction. In this study, we used the linear type smoking machine and Health Canada method for pre-treatment process of phenolic compounds. The analysis time of phenolic compounds using RRLC and monolithic column was individually 8 and 15 minutes, whereas in the conventional HPLC it was 45 minutes. These new methods were accompanied with the minimal solvent consumption and had lower analysis costs. Also, we proved that there were no difference between new methods and conventional method in accuracy by statistic.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.2
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• pp.178-188
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• 2005

To use the filter materials for selective removal of carbonyl compounds in cigarette mainstream smoke, hydrazine such as 2,4-dinitrophenylhydrazine and dansylhydraznie impregnated adsorbents were prepared with perchloric acid or phosphoric acid as a accelerator in hydrazone formation reaction. The change of morphology of adsorbents in various of impregnator were investigated by SEM. Impregnation amount caused by reaction time, acid type and impregnation reagent, and the adsorption properties of carbonyl compounds in cigarette mainstream smoke were investigated. Amounts of impregnation was increased as increasing reaction time. The removal amount for vapor phase carbonyl compounds by 2,4-DNPH impregnated adsorbent was higher than that of dansylhydrazine impregnated adsorbent. The selectivity of 2,4-DNPH impregnated polyacrylic type adsorbent was superior to those of other adsorbents. This results indicated that the 2,4-DNPH impregnated polyacrylic adsorbent was applicable to cigarette filter material because of its fast reactivity and porosity.

• Journal of the Korean Society of Tobacco Science
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• v.30 no.1
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• pp.33-38
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• 2008

This study was carried out to determine the analytical methods for heterocyclic amines(HAs) of the tobacco smoke by LC/MS/MS. HAs have been found in pyrolysate of protein and cooked food including protein, were known the Sugimura compound. HAs content of the smoke were known to exist very low ppb level. Especially, some of HAs are mutagenic and carcinogenic compounds. In according to IARC, the toxicity of N-heterocyclic amines classified IARC class 2A or 2B group. Precursors of these compounds are glutamic acid, protein and free amino acids including tryptophan, therefore, the precursors have been proved in cooked food continuously. This study was investigate multiple analysis methods for HAs and HAs contents of some commercial products. In this study, we used the linear type smoking machine for HAs analysis. At the ISO conditions, mainstream smoke was collected on cambridge filter pad, and then cambridge filter pad was extracted by 0.1% acetic acid. The extracted solution were passed cation exchange SPE cartridge to remove matrix, samples were analyzed using LC/MS/MS on MRM mode. From the result that optimized this methods, the correlation coefficient(R) of the individual compounds were good linearity over 0.999, recovery rate over 96% and the limit of detection were good values between 0.06 to 0.37 ng/mL, In addition, HAs content of some commercial products were in range of 0.02 to 43.8 ng/cig.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.146-151
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• 2007

Gas phase and particulate phase radicals in mainstream cigarette smoke were determined Electron Spin Resonance(ESR) spectroscopy. The free radicals in particulate phase have been investigated by benzene extract of Cambridge Filter Pad containing the smoke condensate. Spin trapping method in conjunction with ESR was used to investigate free radicals in the gas phase of cigarette smoke. Several analytical experiments were conducted in order to determine the optimal conditions for maximum signal intensities and reproducibility of results. All the tests were optimized and normalized using the University of Kentucky 2R4F reference cigarette. The optimal conditions were 0.6 mL for analysis volume of ESR, $4{\sim}5\;mL$ for collection volume of spin-adducts, and PBN for quantification of free radicals in gas phase. The radical levels of Kentucky 2R4F cigarettes were found $2.18{\times}10^{14}\;spins/cig.$ and $2.10{\times}10^{15}\;spins/cig.$ in gas phase.

• Journal of the Korean Society of Tobacco Science
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• v.30 no.1
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• pp.77-84
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• 2008

• Journal of the Korean Society of Tobacco Science
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• v.31 no.2
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• pp.75-84
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• 2009

The objective of this study was to investigate the effect of nitrogen compounds such as protein on the chemical composition and toxicity of cigarette mainstream smoke. BSA protein was treated into the tobacco leaf of original 2R4F cigarette at 1~4 % level. The studies were performed which included a bacterial mutagenicity assay and a mammalian cell cytotoxicity assay for total particulate matter(TPM), and glutathione(GSH) consumption assay for gas/vapor phase(GVP) and determination of smoke chemical constitute. Cigarettes treated with protein were observed dose-dependent increase in yield of volatiles, semi-volatiles and aromatic amines compared with control cigarette. However, carbonyl compounds such as acrolein was lower than that of control cigarette when calculated on an equal TPM basis. The cytotoxicity of TPM obtained from the protein-added cigarettes was not different from that of control cigarette. However, the mutagenicity of the TPM from protein-treated cigarettes(1~4 %) was up to 10-27 % higher than that of control. On the other hand, toxicity of GVP from protein-treated cigarette(4 %) was significantly decreased compared with control cigarette. An overall assessment of our data suggests that nitrogen compounds such as protein should be important for the chemical composition and biological activity of cigarette mainstream smoke.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 2000.05a
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• pp.12-21
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• 2000

The smoke from a burning cigarette is classified as mainstream, which is the smoke inhaled by the smoker during a puff, and sidestream, which is defined by ISO 10185 as all smoke which leaves a cigarette during the smoking process other than from the butt end. Most of the sidestream smoke is generated during static burn, that is, in between puffs. The amount of sidestream smoke generated by a cigarette depends on the cigarette construction, tobacco blend, and properties of the cigarette paper, The main paper properties affecting sidestream smoke generation are: porosity, basis weight, type and amount of filler, type and amount of burn additive.Sidestream smoke is composed of a visible phase (small liquid droplets) and an invisible phase (gaseous molecules). This paper focuses on the visible portion of the sidestream smoke. Optical methods, which are based on the relationship between light scattering and density of the rising plume of smoke, have been used successfully by the industry. However, the present trend is to use gravimetric methods where the particulate matter is captured on a Cambridge(R) filter pad and weighed. The gaseous portion of the sidestream smoke, which does not contribute to the visible sidestream smoke, passes through the Cambridge filter pad.Sidestream smoke reduction is achieved by modifying certain mass transport processes occurring in a smoldering cigarette. There are four main pathways for reducing sidestream smoke: A) less tobacco burned, B) slower rate of tobacco combustion, C) more efficient trapping of smoke by the cigarette paper, and D) more complete combustion of tobacco. This paper discusses how the physical properties of paper and cigarette construction affect sidestream smoke reduction via the above four mechanisms.