The structural components of microorganism are quite related to the toxin and environmental conditions. The vegetative and dormant cells are quite affected by the physical and chemical environments to survive and they will be dormant when they are in the extreme environment. The mechanism to activate the microorganisms however, is not well defined yet in the area of activation state and sporulation state through the analysis of EPS. Other than that even the main mechanism of prior to acquisition of analysis values is not well understood. Therefore, what kind of specific environment to affect the activation and sporulation will be discussed through the analysis of the extracellular polymeric substances(EPS). EPS are a high molecular weight mixture of polymers presenting both outside of cells and interior of microbial aggregates. They are a major complex materials in microbial aggregation for sustaining them together in a three dimensional matrix. Three commonly used extraction methods were applied to this study their effectiveness and quantification in extracting EPS from several Bacillus microorganisms and activated sludge. Three extraction methods used for this study are regular centrifugation with formaldehyde (RCF), Steaming, and EDTA extraction. The results of EPS contents such as the quantitative proteins, carbohydrates and the ratio of protein versus carbohydrate from the several species with the several specific methods showed in this research. This study aims to get comparable results of the quantitative production of EPS and the effectiveness of sedimentation for Bacillus microorganisms and activated sludge from several wastewater treatment plans. The results revealed that the protein amount extracted was the highest by the Steaming method of three extraction methods before sporulation and the carbohydrate amount extracted was the highest by the RCA method of three extraction methods after sporulation. The higher amount of protein compared with carbohydrate from Bacillus microorganisms affected higher sedimentation efficiency, however it could not be found the relation between the EPS production and sedimentation efficiency for the activated sludge.
Proceedings of the Korean Society of Sericultural Science Conference
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1997.06a
/
pp.1-21
/
1997
Silk is praised "Queen of Fiber", which wrote a glorious page in the history of human adornment culture. Thanks to the development of science and technology, silk production has been increased dramatically during last half century. Nowadays, as "Healthy Fiber" silk lets all the globe to be forming the "Silk Boom" Like in other scientific fields, a great deal of progress has been made in sericultural science and technology, which also accelerated the development of multipurpose utilization of sercultural recourses. The main tendency of the sericulture development is raising the quality of cocoon, and at the same time, increasing the output per unit mulberry area and labor production efficiency. The chief measure of raising the cocoon quality is breeding and selecting the new mulberry and silkworm varieties, preventing and curing the diseases and pests and improving the environment of mounting, and the symble for increasing the labor productivity is carrying out the technological system of saving labor and operation ease, and enlarging the production scale. As a natural protein fiber, silk will be still a favorite with human in the next century, because its structure and properties can not be replaced by other any fiber. The 21st century is the biological time, and measures of developing sericulture will use the achievement on the field of molecular biology and biotechnology to carry out the molecular breeding, development regulating, silkworm rearing industrializing, exploiting the new silk and its new usage, utilizing the sericultural resources, and producing the special protein from silkworm as a host of expressing exogenous genes so that make the sericulture to contribute more what it can do for the human being.
The weather has a lot of influence on the cultivation of crops. Weather information on agricultural crop cultivation areas is indispensable for efficient cultivation and management of agricultural crops. Despite the high demand for agricultural weather, research on this is in short supply. In this research, we deal with the production method of agricultural weather in Jeollanam-do, which is the main production area of onions through GloSea5 and deep learning. A deep neural network model using the sliding window method was used and utilized to train daily weather prediction for predicting the agricultural weather. RMSE and MAE are used for evaluating the accuracy of the model. The accuracy improves as the learning period increases, so we compare the prediction performance according to the learning period and the prediction period. As a result of the analysis, although the learning period and the prediction period are similar, there was a limit to reflect the trend according to the seasonal change. a modified deep layer neural network model was presented, that applying the difference between the predicted value and the observed value to the next day predicted value.
Common mushroom production per area has been decreased and are up to less than 50% of the 1980 production. To determine the main reasons for the decrement, we performed this study. Two main reasons, which are mushroom disease and the low compost quality because of mechanized compost making, were assessed. In mechanized mushroom farms, nitrogen concentration in compost was lower than recommended and total compost quantity was about 100-150 $kg/3.3m^2$, which was also lower than usual. Our study revealed that higher nitrogen concentration (about 1.5%) in compost gave better production. Also, use of large amount of compost appeared to increase the mushroom production, although more insects and disease problems were observed. The relationships between the presences of microorganisms and occurrence of diseases were assessed by monitoring the microorganism densities near the mushroom farms. Higher number of microorganisms were observed near the mushroom farm area, compared to control region, Daechon beach. Most contaminating molds were found in the circulating fans, tunnel and culture room floor. The bacterial isolates were collected from the air in mushroom culture room and killed with 0.005% Benzalkonium solution, indicating treatment of Benzalkonium are the effective methods to sanitize the mushroom culture room.
Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.
Coastal sediment archives are used as indicators of changes on shore sediment production and fluvial sediment transport, but rivers crossing coastal plains may not be efficient conveyors of sediment to the coast. In some case there is a net loss of sediment in lower coastal plain reaches, so that sediment input from an upstream exceeds the sediment yield (SY) at the river mouth. The main source of sediment in coastal area is the load from land. In Korea, data on suspended SY are limited owing to a lack of logistic support for systematic sediment sampling activities. This paper presents an integrated approach to estimate SY for ungauged coastal basins, using a soil erosion model and a sediment delivery ratio (SDR) model. For applying the SDR model, a basin specific parameter was validated on the basis of field data. The proposed relationships may be considered useful for predicting suspended SY in ungauged basins that have geologic, climatic and hydrologic conditions similar to the study area.
Seo, Jong Ho;Hwang, Chung Dong;Yi, Hwi Jong;Choi, Weon Young;Bae, Hyun Kyung;Kim, Sang Yeol;Oh, Meong Kyu
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
/
pp.345-345
/
2017
In order to reduce rice cultivation area in paddy fields and to increase domestic self-sufficiency of imported upland grain, it is necessary to increase double cropping area of upland crops in paddy field in accordance with decrease of rice consumption in Korea. The double cropping of spring potato-summer grain maize can be combined because of enough growing season in the southern plain of Korea. Spring potato, which is profitable, can be planted in the late February and harvested in the late May as the main crop. Subsequent grain maize can be planted in early June and harvested in November (maturity in the early October). Spring potato (variety Soomi) yielded $2,544kg\;10a^{-1}$ (tuber) when planted in late February, 2016. When maize was planted in June as the second cropping crop, though growth of plant decreased much, grain yield decreased slightly compared to normal planting in April or May. There was enough time to dry maize ear in the field after maturity before harvesting, which saved labor and time for grain drying, since there is no autumn planting at the double cropping of spring potato-summer grain maize. When grain maize (variety Gwangpyeongok) was planted in the early June (June $10^{th}$), average grain yield of above $860kg\;10a^{-1}$ over 2 years of 2015 and 2016 was obtained, and the annual total yield (potato tuber + maize grain) of 3,400 kg $10a^{-1}$ was obtained. The result indicates that the double cropping of spring potato-summer maize using paddy fields in southern plain of Korea, could contribute to the self-sufficiency of upland crops through the maximum production.
This study was conducted to investigate the production, elver stocking, rearing facilities and rearing method of eel culture to determine aquaculture management conditions for optimal rearing of eel Anguilla japonica. The production of eel culture was evaluated by the proportion of eels from the main inland fin fish species production in Korea. Elver stocking was assessed by the elver stocking densities of pond and recirculation culture. Rearing facilities were investigated according to the rearing tank size proportion of the pond and recirculation culture. We selected sample farms by region and by size. We visited sample farms and recorded the number of elvers stock for pond area, size of tanks, feed and feed quantity, and the size and number of harvest eels. The production capacity of Jeollanam-do and Jeollabuk-do were 71.9% and 21.3% respectively. This production quantity represented 93.2% of the total Korean eel production quantity. In Jeollanam-do, there are 236 eel farms, 202 pond farms, and 34 recirculation aquaculture facilities. The elvers' first density data by each aquaculture method revealed that elvers' first density varied more in recirculation system farms, as compared to pond aquaculture. In intensive pond farms, the elvers' first density decreased as the size of farm increased. There was a correlation between the size of tank(x) and the facility of a water wheel for dissolved oxygen in pond culture systems(y=0.022x-0.494; $R^2$=0.860). Another strong correlation was found between the weight of eel(x) and eel density(y) in pond culture systems(y=283.5x-0.27; $R^2$=0.992). Finally, there was a strong correlation between the length of eel(x) and the weight of eel(y) in intensive pond culture(y=0.0005x-3.2783; $R^2$=0.9775). The final survival rate did not differ significantly among pond sizes and culture types.
China has become the group of two (G2) in almost fields including the scientific technology following the economic growth and joining the WTO in 2001. The main reason is that the government had strong intention for the industrialization of the scientific technology and connected the scientific technology and the economy. Typically, for analyzing the cause of the meteoric rise of China, the competitiveness of the scientific technology was analyzed by the entire score of the nation. However, in the case of China, there are differences in the pattern of the development between the eastern, central, and western province. Also, the industrialization and the competitiveness of the scientific technology are difference because each province established the decentralization of power. Therefore, it is more meaningful to analyze the main factors of Chinese economic growth on a province unit. In this study, therefore, we analyzed the competitive of R&D in China by 124 indexes in 31 areas. The data was analyzed by Partial least squares regression analysis. In conclusion, the scale of the area and the ability of R&D of the company are very important factors for total amount of production in the area. And the journals, patents, the transfer of technical know-how and the investment of R&D are main factors of the amount of export on the high-tech product. According to these results, the factors which make the difference in the industrialization and the competitiveness of the scientific technology in China were analyzed. Finally, it will be helpful to establish the policy for the development of the industrialization and the scientific technology in Korea.
Journal of the Korean Society of Food Science and Nutrition
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v.42
no.3
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pp.457-463
/
2013
The purpose of this study was to apply the Hazard Analysis Critical Control Point (HACCP) system to the production of dressing. The hazard analysis examined the main materials, industrial water, microbial evaluation, and airborne microorganisms of each working area, as well as the pathogenic microbial contamination risk. The survey was conducted at SJ Company in Jincheon (Chungchengbuk-do), Korea for 30 days from April 1, 2012 to April 30, 2012. The results showed that raw material microorganisms had a total plate count in industrial water below $3.00{\times}10$ CFU/mL in working room I, working room II, the packing room, washing water, and the inspection room for five times in each place. During dressing production (including heat treatment and mixing), general bacteria were detected at an average of $3{\times}10$ CFU/mL, but yeast, mold, and pathogenic bacteria were not detected. Airborne microbiological evaluation (for total plate count, yeast, and mold) found levels below the legal limit at each working area. While workers were positive for microbes in total plate counts, coliform and Staphylococcus aureus were not detected. In conclusion, standards for hygienic management should be established to prevent and decrease hazards, such as general bacteria and pathogenic microorganisms (for example, E. coli, B. cereus, Listeria spp, Salmonella spp, Staph. aureus, Clostridium perfringens, yeast, and mold), and to found critical limits for microorganisms with an HACCP system.
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