• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.197-205
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• 2007

In order to take measures for solving conflicts between Korean government such as MOMAF(Ministry of Maritime Affairs and Fisheries) and MAF(Ministry of Agriculture and Forestry) and the relevant industry circles such as KSA(Korea Swine Association) and KODA(Korea Ocean Dumping Association) regarding the prohibition of marine disposal of livestock. excreta wastes from land, the present conditions of marine disposal of livestock excreta wastes and the standpoints of MOMAF, MAF, KSA and KODA were reviewed in Korea.

• Parasites, Hosts and Diseases
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• v.39 no.3
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• pp.261-264
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• 2001

Trichinellosis is a parasitic zoonosis of public health importance. It is caused by Trichinella spiralis which has a wide host range including humans. In the present communication, the ELISA technique was employed on a total of 803 blood samples from 7 selected pig breeding farms in 1996 for diagnosis and surveillance of trichinellosis. Out of the entire 803 samples, nine were found to be suspected while one was positive by ELISA. But western blot analyses employed for further confirmation have shown that all of 10 samples did not react to larval excretory-secretory product antigens. These results indicate that pig breeding farms included in the present study are free from trichinellosis . However, it does not mean Korea is free from trichinellosis since human trichinellosis has recently been reported. The necessity of continued surveillance for trichinellosis in both pigs and wild animals was discussed.

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.201-208
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• 1998

Piglets' diarrhea is one of the major causes of economic loss in Korean swine industry. To prevent this serious and persistant problem, we have developed systemic farm evaluation and analysis program. The program consists of the checklist and the computerized program. The items of the checklist are selected on the basis of various causative factors related with the piglets' diarrhea including sanitational status, nutritional management, or farm environment. The checklist is composed of 30 items including 6 factors on facility, 8 factors on environment, 6 factors on nutrition and 10 factors on sanitation, respectively. Minimum 1 to maximum 10 points was assigned to each item depending on level or status of farm. The scores calculated by sum of points obtained from each item could find out the problems which the farm was confronted with. To investigate the applicability of a program 150 swine farms raising over 500 heads were selected from 7 provinces throughout the country and surveyed. There were significant correlations between the piglets' diarrhea and the scores of the checklist. Based on above results we revised the checklist which consisted of 22 items including 5 factors on facility, 7 factors on environment, 3 factors on nutrition and 7 factors on sanitation. After that we composed a computerized diagram out of each point of an item which showed and indicated weak factors to be improved promptly. Our present study suggested that this newly established swine farm evaluation program would be a very efficient way to find out the problems and support the systemic control program of the piglets' diarrhea for swine farms.

• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.745-750
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• 1998

A sensitive and rapid enzyme immunoassay(EIA) to detect Escherichia coli O157 in ground beef was developed by using a sandwich type assay with polyclonal antibodies to E coli O157. E coli O157 in ground beef could be detected within 15hr, including incubation for 12hr in enrichment broth and 3hr in immunoassay. The EIA could detect $1.3{\times}10^5$ cells of E coli O157/g of ground beef without enrichment. The lowest limit of detection was 0.23 E coli O157 per g of meat after enrichment. Confirmation was required in the positive specimens in the EIA by culture method even though the negative specimens were not. These results suggested that the immunoassay could be a very efficient method for the screening E coli O157 in meat.

• International Journal of Oral Biology
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• v.32 no.1
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• pp.1-5
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• 2007

Receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) induces osteoclast formation from hematopoietic cells via up-regulation of positive regulators, including $NF-{\kappa}B$, c-Fos, microphthalmia transcription factor (Mitf), PU.1, and nuclear factor of activated T cells (NFAT) c1. In addition to the positive regulation by these transcription factors, RANKL appears to regulate negative regulators such as MafB and inhibitors of differentiation (Ids). Ids and MafB are abundantly expressed in osteoclast precursors, bone marrowderived monocyte/macrophage lineage cells (BMMs). Expression levels of these genes are significantly reduced by RANKL during osteoclastogenesis. Overexpression of these genes in BMMs inhibits the formation of tartarate-resistant acid phosphatase (TRAP)-positive multinuclear osteoclasts by down-regulation of NFATc1 and osteoclast-associated receptor (OSCAR), which are important for osteoclast differentiation. Furthermore, reduced expression of these genes enhances osteoclastogenesis and increases expression of NFATc1 and OSCAR. Taken together, RANKL induces osteoclastogenesis via up-regulation of positive regulators as well as down-regulation of negative regulators.

• 전자공학회논문지 IE
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• v.48 no.2
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• pp.19-25
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• 2011

This paper presented neural network modeling method using vehicle data to predict fuel consumption. To acquire data for training and testing the proposed neural network, medium-class gasoline vehicle drove at downtown and parameters measured include speed, engine rpm, throttle position sensor (TPS), and mass air flow (MAF) as input data, and fuel consumption as target data from OBD-II port. Multi layer perception network was used for nonlinear mapping between the input and the output data. It was observed that the neural network model can predict the vehicle quite well with mean squared error was $1.306{\times}10^{-6}$ for the fuel consumption.

• Korean Journal of Veterinary Research
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• v.47 no.3
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• pp.291-298
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• 2007

Olive flounder, Paralichthys olivaceus is one of the most important cultured fish in Korea, its farming has been negatively impacted by viral, bacterial and parasitic diseases. Streptococcal infection was considered as a serious problem because of significant economic losses in olive flounder farm industry. The development and evaluation of vaccine for protection against infection by this agent were required. We evaluated the safety and efficacy of ${\beta}$-hemolytic Streptococcus (S.) iniae vaccine on olive flounder Three hundreds of flounders (weight $119.8{\pm}20.7g$, body length $22.6{\pm}1.4cm$) were reared in 0.5 tons aquaria in land-marine tank system. Seawater was provided from the sea of Inchon in Korea, and water temperature was set to $22^{\circ}C$ and $25^{\circ}C$ in the vaccination and challenge test, respectively. We used the formalin-inactivated ${\beta}$-hemolytic S. iniae (F2K) vaccine (M VAC INIAE; Kyoritsu seiyaku, Japan) originated in Japan. The vaccine was intraperitoneally administered to fish. Both of vaccinated group and control group were challenged with intraperitoneally injection by virulent S. iniae SI-36 isolates with $1.0{\times}10^7CFU/fish$ at 3 weeks after vaccination. Difference on mortality of control and vaccinated group (90.0 and 15.0%, 76.5 and 8.0% respectively) at two trials were found significant (p<0.05), and relative percent survival were 83.4% and 89.5%, respectively. The dead fishes were showed dark pigmentation of skin, abdominal extension, hemorrhagic ascites, and liver necrosis, and isolated the S. iniae strain from ascites, liver and kidney. We confirmed the safety and efficacy of ${\beta}$-hemolytic S. iniae vaccine by determinations of the optimal management condition and artificial challenge test in olive flounder.

• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.771-776
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• 1998

Synthetic oligonucleotide primers of 20 and 21 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the mrp gene, which encodes the muramidase released protein of Streptococcus suis. Amplification was not recorded when 5 other streptococcal species were tested or when 9 different nonstreptococcal species were tested. A DNA fragment of 517bp was amplified from the genomic DNA of S suis. The lower detection limit was 100pg of the genomic DNA. The primers recognized 34 serotypes of S suis reference strains and 9 isolates from pneumonic lung, brain, nasal discharge, tonsil. This results suggest that the amplification of the mrp gene by PCR method is potential for the identification of S suis isolates.

• Biomedical Science Letters
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• v.18 no.3
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• pp.193-200
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• 2012

We examined the effects of polyamines such as putrescine and cadaverine on the biosynthesis and secretion of insulin in the mouse pancreatic ${\beta}$-cell line, MIN-6. Basal insulin secretion (BIS) and glucose-stimulated insulin secretion (GSIS) from the MIN-6 cells were significantly increased by 20 min- or 24 h-treatment with micromolar concentrations of polyamines. To determine whether the enhancement was due to increase of insulin production by polyamines, we investigated the insulin mRNA and protein production. Both insulin mRNA and protein production were found to be not significantly affected by the polyamine treatment. Next, we examined the expression of several transcription factors (TFs) related to insulin synthesis and secretion in order to identify upstream events responsible for the promotion of insulin secretion of MIN6 cells by polyamines. Of the 6 TFs tested, MafA was induced by treatment of polyamines. MafA mRNA and protein expressions increased with treatment of polyamines. Overall results suggest that cadaverine and putrescine promote the insulin secretion process rather than the insulin biosynthesis from MIN6 cells. Also MafA may be involved in the enhanced insulin secretion process. Further studies are needed to elucidate the underlying mechanisms for promotion of insulin secretion by polyamines.

• Restorative Dentistry and Endodontics
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• v.19 no.1
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• pp.73-84
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• 1994

Using a model system that can compare the before and after of canal preparation in the same tooth, we measured the area of the cross section, and canal wall thickness of the distal portion of the mesial root of the mandibular molar, and compared the amount of reduction in the canal using hand flared preparation the Gates-Glidden drill flared preparation according to the changes in the MAF. The results were as follows. 1. After canal preparation, the canal wall thickness had no significant difference between the hand flared preparation and Gates-Glidden drill flared preparation. 2. The canal wall thickness, after canal preparation, there was no significant difference between the sizes of the MAF. 3. The area variation range of each cross section of root had no significant difference between MAF size and methods of canal preparation. 4. After canal preparation, the frequency of the canal wall thickness under 0.5mm showed 3.5mm below the furcation to be the most frequent with statistical significance(p<0.05). 2mm below the furcation and 5mm below the furcation followed but there was no statistical significance. 5. The danger zone of the mesial root of the mandibular molar seems to be around 3.5mm.