• Biomolecules & Therapeutics
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• 제31권3호
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• pp.340-349
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• 2023

Mad2B (Mad2L2), the human homolog of the yeast Rev7 protein, is a regulatory subunit of DNA polymerase ζ that shares sequence similarity with the mitotic checkpoint protein Mad2A. Previous studies on Mad2B have concluded that it is a mitotic checkpoint protein that functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C). Here, we demonstrate that Mad2B is activated in response to cisplatin-induced DNA damage. Mad2B co-localizes at nuclear foci with DNA damage markers, such as proliferating cell nuclear antigen and gamma histone H2AX (γ-H2AX), following cisplatin-induced DNA damage. However, unlike Mad2A, the binding of Mad2B to Cdc20 does not inhibit the activity of APC/C in vitro. In contrast to Mad2A, Mad2B does not localize to kinetochores or binds to Cdc20 in spindle assembly checkpoint-activated cells. Loss of the Mad2B protein leads to damaged nuclei following cisplatin-induced DNA damage. Mad2B/Rev7 depletion causes the accumulation of damaged nuclei, thereby accelerating apoptosis in human cancer cells in response to cisplatin-induced DNA damage. Therefore, our results suggest that Mad2B may be a critical modulator of DNA damage response.

• The Korean Journal of Physiology and Pharmacology
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• 제27권5호
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• pp.427-436
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• 2023

Mitotic arrest deficient 2 like 2 (Mad2L2, also known as Mad2B), the human homologue of the yeast Rev7 protein, is a regulatory subunit of DNA polymerase ζ that shares high sequence homology with Mad2, the mitotic checkpoint protein. Previously, we demonstrated the involvement of Mad2B in the cisplatin-induced DNA damage response. In this study, we extend our findings to show that Mad2B is recruited to sites of DNA damage in human cancer cells in response to cisplatin treatment. We found that in undamaged cells, Mad2B exists in a complex with Polζ-Rev1 and the APC/C subunit Cdc27. Following cisplatin-induced DNA damage, we observed an increase in the recruitment of Mad2B and Cdc20 (the activators of the APC/C), to the complex. The involvement of Mad2B-Cdc20-APC/C during DNA damage has not been reported before and suggests that the APC/C is activated following cisplatin-induced DNA damage. Using an in vitro ubiquitination assay, our data confirmed Mad2B-dependent activation of APC/C in cisplatin-treated cells. Mad2B may act as an accelerator for APC/C activation during DNA damage response. Our data strongly suggest a role for Mad2B-APC/C-Cdc20 in the ubiquitination of proteins involved in the DNA damage response.

• 한국통신학회논문지
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• 제24권8B호
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• pp.1506-1517
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• 1999

본 논문에서는 이웃 블록과 중첩된 움직임 탐색영역에서의 MAD 분포 및 블록내 화소의 부표본화를 이용한 두가지 고속 움직임 추정 방법을 제안하였다. 제안한 두 가지 방법에서는 이웃 블록과 중첩된 영역에서 현재 블록 MAD의 최소 및 최대 범위를 구하여 이용하였으며, 이때 사용되는 MAD의 최소 및 최대 범위는 이웃 블록의 MAD와 현재 블록과 이웃 블록간의 MAD를 이용하여 구하였다. 또한, 블록 정합은 블록내 화소를 부표본화한 뒤 행하였다. 제안한 첫 번째 방법에서는 블록 MAD의 최소 범위를 이용하여 이웃 블록과 움직임이 중첩되지 않는 영역에서 구한 기준 MAD가 블록 MAD의 최소 범위보다 큰 탐색점에 대하여서만 블록 정합을 행함으로써 고속으로 움직임을 추정하였다. 또한, 본 논문에서는 계산량을 크게 줄이기 위한 방법으로 현재 블록 MAD의 분포 특성을 이용한 고속 움직임 추정 방법을 제안하였다. 이 방법에서는 이웃 블록과 중첩된 각 탐색점에서의 실제 MAD는 MAD의 최소 및 최대 범위 사이에 존재하게 된다는 것을 이용하여, 실제 MAD 분포의 확률적인 모델을 이용하여 고속으로 움직임을 추정하였다. 제안한 방법의 성능을 평가하기 위한 컸뼜\ulcorner모의 실험결과로부터 제안한 방법이 움직임 추정 오차 측면에서 우수한 성능을 유지하면서도 계산량을 현저히 줄일 수 있음을 확인할 수 있었다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2005년도 춘계학술대회논문집
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• pp.83-86
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• 2005

The aim of this study is to establish the indoor noise limits of apartment houses for mad traffic noise. To achieve this goal, psycho-acoustic experiments were carried out with the mad traffic sound sources modulated by the transmission loss characteristics of the external windows. As a result of this study, followings are suggested. 1) On correlation between dose level and psycho-acoustical response, the initial level of negative feeling is located on $40.1{\sim}40.6$ Leq dB(A). 2) On the degree of satisfaction to road traffic noise, near 35% Point being same dissatisfaction degree is to be assumed $40{\sim}41$ dB(A) of indoor noise level presented into three vocabulary. It is suggested to be reasonable level of 40 dB(A) on the indoor noise limits for intruding road traffic noise, and it is appropriate to be the 5dB level difference between grades.

• 생명과학회지
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• 제16권5호
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• pp.828-833
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• 2006

옥수수 (Zea mays L.) 꽃은 암술 세포사멸과 수술 세포 성장정지 등을 통하여 양성상태에서 단성 상태로 성결정 과정을 완성한다. 본 논문에서는 옥수수 성 결정 동안 세포주기 유전자들의 시간적, 공간적 발현조절을 조사하였다. 세포주기의 양성조절 인자 즉 cyclin A, cyclin B, cyclin dependent kinase A (CDK A), Mad2 유전자들은 성장하는 암술과 수술에서 높게 발현되는 반면 죽어가는 암술과 성장이 정지되는 수술에서는 이들의 발현이 사라졌다. 이와 반대로, Wee1과 CDK inhibitor (CKI) 같은 세포주기 음성 조절유전자들은 야생형 암꽃과 tasselseed2 돌연변이 수꽃의 성장이 정지하고 있는 수술에서 발현이 증가되었지만, 흥미롭게도, 이들 유전자들은 죽어가는 암술세포에서는 발현되지 않았다. 이들 결과들을 통하여 옥수수 성 결정 과정 중에서 암술 세포사멸과 수술세포 성장정지는 세포주기조절과 밀접한 관계가 있으며, 특히 성장이 정지하는 수술과 죽어가는 암술에서의 음성 세포주기 조절 유전자들의 다른 발현양상은 이 둘의 성 결정 메커니즘이 구별 될 것이라고 사료된다.

• 천문학회지
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• 제33권1호
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• pp.47-55
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• 2000

Nonpotential characteristics of magnetic fields in AR 5747 are examined using Mees Solar Observatory magnetograms taken on Oct. 20, 1989 to Oct. 22, 1989. The active region showed such violent flaring activities during the observational span that strong X-ray flares took place including a 2B/X3 flare. The magnetogram data were obtained by the Haleakala Stokes Polarimeter which provides simultaneous Stokes profiles of the Fe I doublet 6301.5 and 6302.5. A nonlinear least square method was adopted to derive the magnetic field vectors from the observed Stokes profiles and a multi-step ambiguity solution method was employed to resolve the $180^{\circ}$ ambiguity. From the ambiguity-resolved vector magnetograms, we have derived a set of physical quantities characterizing the field configuration, which are magnetic flux, vertical current density, magnetic shear angle, angular shear, magnetic free energy density, a measure of magnetic field discontinuity MAD and linear force-free coefficient. Our results show that (1) magnetic nonpotentiality is concentrated near the inversion line in the flaring sites, (2) all the physical parameters decreased with time, which may imply that the active region was in a relaxation stage of its evolution, (3) 2-D MAD has similar patterns with other nonpotential parameters, demonstrating that it can be utilized as an useful parameter of flare producing active region, and (4) the linear force-free coefficient could be a evolutionary indicator with a merit as a global nonpotential parameter.

• 한국산림과학회지
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• 제108권4호
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• pp.592-599
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• 2019

본 연구는 수간재적과 상관관계가 높은 흉고직경과 수고를 독립변수로 하여 우리나라 일본잎갈나무의 수간재적 추정식을 개발하고자 하였다. 전국의 분포하고 있는 일본잎갈나무를 총 2,840본의 표준목을 벌채하여 수간고별 직경과 수간석해 자료를 수집하여 생장특성을 조사하였다. 6개의 수간재적모델의 적합도 검정을 위하여 적합도지수, 편차, 평균절대편차, 변이계수를 이용하여 모델의 적합성을 판단하였다. 최종 선정된 모델은 흉고직경만을 이용한 Model 1(V=a+bDBH²), 흉고직경과 수고를 이용한 Model 4(V=a+bDBH²H)가 최적의 모델로 선정되었다. 비선형회귀분석을 통한 모델의 절편과 기울기계수인 a, b는 0.05수준에서 통계적으로 유의한 값을 보였다. 적합도지수는 94%~99%로 나타났고, 편차는 두 모델이 0에 근접하였으며, 평균절대편차는 0.01~0.05, 변이계수는 5.97~14.43으로 높은 적합도를 보였다. 본 연구에서 제시된 수간재적모델을 이용하여 현장에서 흉고직경 또는 흉고직경과 수고를 측정한 후 바로 재적을 추정할 수 있으며, 산림경영에 필요한 기초 정보를 제공해 줄 것이다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.162-172
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• 2002

Schizosaccharomyces pombe is suited for the study of cytokinesis as it divides by forming a septum in the middle of the cell at the end of mitosis. To enhance our understanding of the cytokinesis, we have carried out a genetic screen for temperature-sensitive S. pombe mutants that show defects in septum formation and cell division. Here we present the isolation and characterization of a new temperature-sensitive mutant, sun1(septum uncontrolled), which undergoes uncontrolled septation during cell division cycle at restrictive temperature $(37^{\circ}C)$. In sun1 mutant, actin ring and septum are positioned at random locations and angles, and nuclear division cycle continues. These observations suggest that the sun] gene product is required for the proper placement of the actin ring as well as precise septation. The sun] mutant is monogenic recessive mutation unlinked to previously known various cdc genes of S. pombe. In a screen for $sunl^+$ gene to complement the sun] mutant, we have cloned a gene, $susl^+$(suppressor of sun1 mutant), that encodes a protein of 689 amino acids. The predicted amino acid sequence of $susl^+$ gene is similar to the human hMadlp and Saccharomyces cerevisiae Mad1p, a component of the spindle checkpoint in eukaryotic cells. The null mutant of $susl^+$ gene grows normally at various temperatures and has the increased sensitivity to anti-microtubule drug, while $susl^+$ mutant shows no sensitivity to microtubule destabilizing drugs. The putative S. pombe Sus1p directly interacts with S. pombe Mad2p in yeast two-hybrid assays. These data suggest that the newly isolated susr gene encodes S. pombe Mad1p and suppresses sun] mutant defective in controlled septation in a cell division cycle.

• Parasites, Hosts and Diseases
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• 제57권2호
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• pp.185-189
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• 2019

To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.

• ETRI Journal
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• 제18권2호
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• pp.61-74
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• 1996

We propose two new rate control schemes to improve MPEG-2 rate control in view of visual quality when scene changes happen. Two proposed schemes are characterized by real-time and non real-time improvement to reduce the impact of scene changes. We also propose a new target-bit prediction method using spatial activity of pictures and present a simple and efficient scene change detection scheme using signed difference of mean absolute difference (MAD). Computer simulation results show that the proposed real-time algorithm effectively alleviates visual quality degradation after scene changes. The proposed non real-time algorithm gives maximum 2 dB improvement in peak signal-to-noise ratio (PSNR) at a scene-changed picture, compared with MPEG-2 rate control scheme and it shows better quality than the real-time one.