• Title/Summary/Keyword: Macronutrients

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Physiological and Proteomics Analysis to Potassium Starvation in Rice

  • Kim, Sang-Gon;Wang, Yiming;Lee, Chang-Hoon;Chi, Yong-Hun;Kim, Keun-Ki;Choi, In-Soo;Kim, Yong-Chul;Kang, Kyu-Young;Kim, Sun-Tae
    • Korean Journal of Environmental Agriculture
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    • v.30 no.4
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    • pp.395-401
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    • 2011
  • BACKGROUND: Potassium (K) is one of the macronutrients which are essential for plant growth and development. Its deficiency in paddy soils is becoming one of the limiting factors for increasing rice yield in Asia. METHODS AND RESULTS: To investigate physiological symptoms under K-starvation (NP) compared with complete media (NPK) condition, we measured shoot/root length, weight, nutrients, and patterns of protein expression. The shoot growth was significantly reduced, but root growth was not affected by K-starvation. However, biomasses were decreased in both shoot and root. Uptake of K was reduced up to 85%, while total concentrations of P, Ca, Mg, Na were increased in root and shoot. To better understand the starved K mechanism of rice, comparative proteome analysis for proteins isolated from rice leaves was conducted using 2-DGE. Five spots of differentially expressed proteins were analyzed by MALDI-TOF MS. Analysis of these K-starvation response proteins suggested that they were involved in metabolism and defense. CONCLUSION(s): Physiological and 2-DGE based proteomics approach used in our study results in observation of morphology or nutrients change and identification of K-starvation responsive proteins in rice root. These results have important roles in maintaining nutrient homeostasis and would also be useful for further characterization of protein function in plant K nutrition.

Comparison of Biochemical Composition and Antimicrobial Activity of Southern-Type Garlic Grown in the Eastern and Western Regions of Jeju (제주 동부와 서부지역 남도마늘의 생화학적 성분 및 항균활성 비교)

  • Kim, Ju-Sung;Ra, Jong Hwan;Hyun, Hae-Nam
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.763-771
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    • 2015
  • Components and physiological activity of crops are strongly affected by the natural environments of the growth regions. In this study, we carried out soil analysis and determined the allicin, alliin, total pyruvate, total phenol and flavonoid, and reducing sugar contents of garlic from garlic-cultivating regions in the eastern and western portions of Jeju Province. Significantly higher contents of macronutrients were found in the soil and garlic from the eastern region of Jeju compared to western region, but micronutrients were higher in the western region than eastern region. In addition, the alliin and total phenolic contents were higher in the western region than in the eastern region. Also, allicin, total pyruvate, total flavonoid content, and ${\alpha}$-glucosidase inhibitory activity of the eastern region samples were higher than those of the western region in Jeju. This study promotes our understanding of the different components of garlic according to the cultivation regions of Jeju.

Assessment of Dietary Intake and Plasma Lipid Profiles by Age Groups of Korean Men

  • Kim, Hee-Seon
    • Journal of Community Nutrition
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    • v.3 no.1
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    • pp.14-20
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    • 2001
  • A cross-sectional study to determine dietary intake and plasma triglyceride total cholesterol LDL-cholesterol and HDL-cholesterol of 185K Korean men was conducted across three different age groups The younger group (age 21 to 34) was significantly (p<0.001) taller but showed lower (p<0.05) percent body fat than the older group (age 45 to 60) Weight and body mass index was not different among age groups. Older men showed significantly(p<0.01) lower energy and total fat intake than younger men Besides macronutrients, most participants consumed an adequate amount of micronutrients but calcium consumption of the middle age group (age 35 to 44) was less than 75% of RDA In older men plasma triglyceride(207.8$\pm$155.5 mg/dl) total cholesterol (201.4$\pm$40.0 mg/dl) and LDL-cholesterol(106.0$\pm$32.7 mg/dl) concentrations were significantly hight(p<0.001) than in younger men wereas no significant difference was observed in HDL-cholesterol concentration Subjects with a higher BMI(bMI$\geq$25.0) showed significantly higher (p<0.001) triglyceride(200.2$\pm$107.6 mg/dl) total cholesterol(211.0$\pm$40.1 mg/dl) LDC-cholesterol(118.16$\pm$35.5 mg/dl) concentrations and lower(p=0.001) HCL-cholesterol concentration (52.8$\pm$15.9 mg/dl) than subjects with lower BMI(BMI<23.0) Dietary intake of fat cholesterol did not show significant associations with any of the plasma lipid profiles. However, anima fat intake was significantly (p<0.05) correlated with plasma total cholesterol and triglyceride concentrations in the older age group. On the hand percent body fat was correlated (p<0.05) with all of the plasma lipid and lipoprotin concentrations examined for all age groups Results indicate both dietary intake and percent body fat are important determinants of the plasma lipid concentrations is the elderly but only percent body fat or body mass could be valid predictors for the plasma lipid concentrations of the younger age group.

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Factors influencing Adventitious Shoot induction from Leaves of Populus deltoides Bartr (미류나무 잎절편체 조직배양을 통한 식물체 재분화에 미치는 요인)

  • 신동일;설일환
    • Journal of Life Science
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    • v.7 no.2
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    • pp.134-141
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    • 1997
  • We have investigated several factors influencing adventitious shoot induction from the Populus deltodies bartr. leaf segments. To obtain in vitro materials, at first, stem segments from ex vivro were tested for axillary bud breaking on the five macronutrients levels of two differenr media. WPM was better both in bud breaking and leaf expansion than MS medium. The amount of NH$_{4}$NO$_{3}$ added in the medium did not seem to affect axillary bud breaking, significantly and subsequent shoot elongation from the stem segments of P. deltodies. However, other components in WPM might have played important roles in axillary bud breaking and shoot elongation. Regenerability from three sections (the distal, the middle and the base) of leaves cultured on WPM supplemented with TDZ and NAA combinations appeared to be different; middle and basal sections of leaves produced more organogenic sites than those of top section on WPM and those sesctions produced the highest ogranogenic sites on the same medium supplemented with 0.01 mg/l TDZ and 0.02mg.l NAA. Among 3 carbon sources tested for adventitious shoot elongation, fructose seemed to be stimulating the elongation of adventitious shoots. Sucrose and glucose added in the medium resulted in the necrosis which caused dying of adventitious shoots, eventually.

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Relation of Mineral Nutrition Status and Climacteric Symptoms in Pre- and Postmenopausal Women (폐경기를 전.후한 중년 여성의 무기질영양상태와 갱년기증상)

  • Kim Soon-Kyung;SunWoo Jae-Gun;Lee Eun-Ju
    • Journal of Nutrition and Health
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    • v.39 no.2
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    • pp.121-132
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    • 2006
  • Minerals are extremely important in human metabolism, growth, and tissue repair. The risks of disturbances in mineral nutrition and metabolism are high following menopause. The aim of the study was to investigate the mineal nutrition status in middle-aged women (Pre & Postmenopausal) and the relation to climacteric symptoms. In the present study, we conducted the anthropometric measurement, climacteric symptoms survey and the dietary intakes including macronutrients, vitamins and minerals to assess the nutrient intakes. Also plasma mineral levels (Ca, P, Mg, Na, K, Fe, Cu, Zn) was measured. The participants were 105 middle-aged women (pre=51, Postmenopausal=54). The anthropometric measurement showed that 74% of subjects was overweight and obesity. The energy intakes were 83% of Korean EER. The macromineral intake (Ca, P, Mg, Na, K), Ca, P and Mg were 65%, > 116% and > 107% of Korean RI. Na and K intakes were > 243% and 53% of Korean AI. For the trace mineral intake (Fe, Cu, Zn), Fe, Cu and Zn intakes were 82%, > 146%, and 94% of Korean RI. The serum mineral concentration measurement, in case of Ca, Fe and Zn, > 10% of subjects showed lower level than normal clinical range. The most frequently appeared climacteric symptoms were dry eye, forgetfulness, difficult concentration, shoulder pain, fatique, depression & lonesomeness, backache, snore, dry skin, ankle & knee pain. In relation to mineral nutrition with climacteric symptoms, significant correlations have been founded between Ca intake and difficult concentration, Mg intake and snore, Fe & Zn intake and forgetfulness & dry skin, serum Ca concentration and forgetfulness, serum Na concentration and dry eyes & snore, serum K concentration and shoulder pain. From these results, it has been indicated that some mineral nutrition status may effect the prevention and reduction of climacteric symptoms in pre and postmenopause women.

Differences in Nutrient Intake with Homemade versus Chef-Prepared Specific Carbohydrate Diet Therapy in Inflammatory Bowel Disease: Insights into Dietary Research

  • Morrison, Alex;Braly, Kimberly;Singh, Namita;Suskind, David L.;Lee, Dale
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.24 no.5
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    • pp.432-442
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    • 2021
  • Purpose: The aim of this study was to evaluate the nutrient content consumed by children and adolescents on home-prepared versus chef-prepared specific carbohydrate diets (SCD) as therapy for inflammatory bowel disease (IBD). Methods: Dietary intake of two cohorts with active IBD initiating the SCD over 12 weeks was assessed. The home-prepared cohort received detailed guidance from dietitians on implementation of the SCD. The chef in the other cohort was knowledgeable in the SCD and prepared meals from a fixed set of recipes. Data from 3-day diet diaries at 4 different time points were collected. US Recommended Daily Allowances (RDA) were calculated for macronutrients, vitamins, and minerals. Results: Eight participants on the homemade SCD and 5 participants on the chef-prepared SCD were included in analysis. Mean % RDA for energy intake was 115% and 87% for homemade and chef-prepared groups (p<0.01). Mean % RDA for protein intake was 337% for homemade SCD and 216% for chef-prepared SCD (p<0.01). The homemade SCD group had higher mean % RDA values for vitamin A and iron, while the chef-prepared SCD group had higher intake of vitamins B1, B2, D, phosphorus and zinc (p<0.01 for all). Conclusion: The SCD implemented homemade versus chef-prepared can result in significantly different intake of nutrients and this may influence efficacy of this dietary therapy. Meal preparation dynamics and the motivation of families who pursue dietary treatment may play an important role on the foods consumed and the outcomes on dietary therapy with the SCD.

Nitrate supplementation of rations based on rice straw but not Pangola hay, improves growth performance in meat goats

  • Paengkoum, Siwaporn;Khotsakdee, Jiravan;Paengkoum, Pramote;Schonewille, Thomas;Yuangklang, Chalermpon
    • Animal Bioscience
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    • v.34 no.6
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    • pp.1022-1028
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    • 2021
  • Objective: Supplemental nitrate is known to be an effective tool to mitigate methane emission by ruminants. Based on theoretical considerations, supplemental nitrate can improve but also deteriorate the growth performance. The overall effect of supplemental nitrate on growth performance, however, is not yet known. The objective of the current study was therefore to evaluate the effect of a higher dose of NO3- on overall growth performance when feeding either Pangola grass hay or rice straw. Methods: Thirty-two crossbred, 3-month-old Thai native×Anglo-Nubian crossbred male goats were used. The experiment had a 2×2 factorial design with an experimental period of 60 days. Eight goats were randomly allocated to each dietary treatment, i.e. a ration containing either Pangola hay (Digitaria eriantha Steud) or rice straw (Oryza Sativa) as a source of roughage, supplemented with a concentrate containing either 3.2% or 4.8% potassium nitrate. The rations were formulated to be isonitrogenous. The animals were weighed at the start of the experiment and at days 30 and 60. Feces were collected during the last five days of each 30-day period. Results: High-nitrate increased overall DM intake by approximately 3%, irrespective the source of roughage, but only the goats fed a rice straw-based ration responded with an increase in body weight (BW). Thus, the overall feed conversion ratio (kg feed/kg BW gain) was influenced by roughage source ×nitrate and decreased by almost 60% when the goats were fed rice straw in combination with a high versus a low dietary nitrate content. The digestibility of macronutrients was only affected by the source of roughage and the digestibility of organic matter, crude protein, and neutral detergent fibre was greater when the goats were fed Pangola hay. Conclusion: It was concluded that the replacement of soybean meal by nitrate improves the growth performance of meat goats fed rations based on rice straw, but not Pangola hay.

Crosstalk of Zn in Combination with Other Fertilizers Underpins Interactive Effects and Induces Resistance in Tomato Plant against Early Blight Disease

  • Awan, Zoia Arshad;Shoaib, Amna;Khan, Kashif Ali
    • The Plant Pathology Journal
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    • v.35 no.4
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    • pp.330-340
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    • 2019
  • The present study was undertaken to evaluate the integrated effect of zinc (Zn) with other nutrients in managing early blight (EB) disease in tomato. A pot experiment was carried out with basal application of the recommended level of macronutrients [nitrogen, phosphorus and potassium (NPK)] and micronutrients [magnesium (Mg) and boron (B)] in bilateral combination with Zn (2.5 and 5.0 mg/kg) in a completely randomized deigned in replicates. Results revealed that interactive effect of Zn with Mg or B was often futile and in some cases synergistic. Zn with NPK yield synergistic outcome, therefore EB disease was managed significantly (disease incidence: 25% and percent severity index: 13%), which resulted in an efficient signaling network that reciprocally controls nutrient acquisition and uses with improved growth and development in a tomato plant. Thus, crosstalk and convergence of mechanisms in metabolic pathways resulted in induction of resistance in tomato plant against a pathogen which significantly improved photosynthetic pigment, total phenolics, total protein content and defense-related enzymes [superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL)]. The tremendous increase in total phenolics and PAL activity suggesting their additive effect on salicylic acid which may help the plant to systemically induce resistance against pathogen attack. It was concluded that interactive effect of Zn (5.0 mg/kg) with NPK significantly managed EB disease and showed positive effect on growth, physiological and biochemical attributes therefor use of Zn + NPK is simple and credible efforts to combat Alternaria stress in tomato plants.

Intraspecific variations in macronutrient, amino acid, and fatty acid composition of mass-cultured Teleaulax amphioxeia (Cryptophyceae) strains

  • Lee, Bae Ik;Kim, Shin Kwon;Kim, Jong Hyeok;Kim, Hyung Seop;Kim, Jong Im;Shin, Woongghi;Rho, Jung-Rae;Yih, Wonho
    • ALGAE
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    • v.34 no.2
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    • pp.163-175
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    • 2019
  • To compare the nutritional quality of TPG (Teleaulax / Plagioselmis / Geminigera) clade species of cryptomonads with that of RHO (Rhodomonas / Rhinomonas / Storeatula) clade species 6 Teleaulax amphioxeia (TA) and 1 Rhinomonas sp. strains were mass-cultured in newly designed 500-L photobioreactors to the end of exponential growth phase. Intraspecific variations (IVs) in terms of one standard deviation among the 6 TA strains in the compositions of the three macronutrients were 41.5 (protein), 89.8 (lipid), and 15.6% (carbohydrate) of the mean. When harvested from stationary growth phase mean compositions of essential amino acids (EAAs, 47.3%) and non-EAAs (52.7%) of the 2 TA strains, CR-MAL07 and CR-MAL08-2, were similar to those of a Chroomonas strain. The IVs between the 2 TA strains in the composition of EAAs (10.3 and 2.4) and non-EAAs (8.5 and 2.1% of the mean) were rather smaller than those of saturated fatty acids (30.3 and 26.1) and unsaturated fatty acids (UFAs, 12.0 and 12.5% of the mean) in f/2-Si and urea-based compound fertilizer (UCF) culture media, respectively. Mean compositions of eicosapentaenoic acid (EPA, 17.9%) and docosahexaenoic acid (DHA, 12.7%) of total fatty acids of the 2 TA strains were higher than those that of a Chroomonas strain. EPA and DHA compositions exhibited similar level of IVs between the 2 TA strains in f/2-Si (14.6 and 11.0) and UCF media (12.6 and 13.5% of the mean). Thus, the nutritional quality in terms of amino acids, UFAs, EPA, and DHA in a TPG clade species, T. amphioxeia was comparable to those of RHO clade species with notable IVs. Practically, biotechnological targets for TPG clade cryptomonad strains might be subspecies or clone level.

Workplace foodservice meals are associated with higher nutritional quality among adult Korean workers: a nationwide cross-sectional study

  • Shin, Woo-young;Cho, Soo Hyun;Kim, Jung-ha
    • Nutrition Research and Practice
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    • v.16 no.2
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    • pp.248-259
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    • 2022
  • BACKGROUND/OBJECTIVES: Adequate nutritional intake is essential for good health and well-being. We aimed to compare the nutrient intake from homemade meals, meals sourced externally ("eating out"), and meals from workplace foodservices, as well as the potential association between foodservices used and nutritional quality among adult Korean workers. SUBJECTS/METHODS: We used data from the Korea National Health and Nutrition Examination Survey, a nationwide cross-sectional survey on the health and nutritional status of the Korean population. Data from 6,845 workers aged 20-64 years were included. A survey on dietary behavior and nutrition was conducted using the 24-h dietary recall method. Nutritional quality was examined using the index of nutritional quality (INQ) score. Higher INQ scores reflected poorer nutritional quality, with insufficient intake of a higher number of nutrients. Multivariate linear regression was used to identify the association between the foodservices used and INQ scores after adjusting for other covariates. RESULTS: We found that foodservice users exhibited a higher 1-day intake of total energy and macronutrients (all P < 0.05) and lower INQ scores (P < 0.01) than non-users. The INQ scores for total daily meals decreased with the frequency of foodservice use (P < 0.01). A significant association was observed between the non-use of foodservices and INQ scores (β = 0.29, P < 0.01) after adjusting for other covariates. CONCLUSIONS: The use of workplace foodservices contributed to higher dietary nutritional quality among workers. This study highlights the importance of expanding the scope of workplace foodservices to promote higher nutritional quality and healthy dietary habits among workers.