• Analytical Science and Technology
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• v.19 no.4
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• pp.290-300
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• 2006

Disinfection by-products(DBPs), such as volatile trihalomethanes and the nonvolatile organochlorine acids, created by chlorination have been extensively studied. However MX which contributes 20-50% of the mutagenic activity in drinking water began to people's attention since 1990. Its chemical name is 3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone. According to WHO guidelines its concentration should be controlled, but its value has not been set up. Due to analytical difficulties in measuring this compound at such a low concentrations and lack of information on toxicity to human. Because concentration (ng/L) of MX in drinking water is low traditional testing methods are ineffective. Therefore this study compared LLE and SPE and have chosen SPE to improve preconcentration. MX has been identified in chlorinated drinking water samples in several countries but not in korea Therefore this study analyzed concentration of MX in different water sources and in spring water. This study examined the causes of changing MX content. Chlorine dosage, seasons, water temperature and distance from the source was all discoverd to be relavant. MX was analyzed in various treatment to find optimum disinfection methods. The outcome was that the concentration of MX was minimized when using biological activated carbon-O3 and granular activated carbon.

• Biomedical Science Letters
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• v.16 no.4
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• pp.229-238
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• 2010

Chicken Mx protein (cMx) induced interferon (IFN) is an antiviral protein to inhibit replication of RNA virus, particularly negative stranded RNA virus, through blockage of transfortation of viral RNA and proteins. In order to determine antiviral effects of cMx from different MHC haplotype chicken, we characterized cMx gene by studying on nucleotide sequencing, antiviral effects to Newcastle disease virus, VSV and MDV, and transcription activities. Three types of eMx genes (2,118 bp) were detected from the different MHC haplotype chickens [B19 (N), B15(F) and B21 (GSP)] chickens, which have showed different susceptible to Marek's disease (MD). Several amino acid substitutions were showed in the cMx. The amino acid 548 and 631 in the cMxs from N and F, chickens susceptible to MD, was Val and Asn which was important on antiviral effects, and showed in resistant cMx. Those in the cMx from GSP, chicken resistant to MD, were same that showed in susceptible cMx. Though every cMx transactivated the expression of the reporter gene, the transcription activation by resistant cMx from N and F was lower compared to that by susceptible cMx from GSP. The decease of the cell growth in the resistant cMx cloned cells was seen in comparison with another cMx clone cells. Replication of NDV and VSV was suppressed in the clones with resistant cMx from N and F. NMx258-transducted cells lack of antiviral effects, and NMx437 or NMx646-transducted cells was showed 60% of antiviral effects compared to NMx705. Mean death time (MDT) and hemaggutination (HA) titer to NDV was long and low in the eggs of N and F lines, but short and high in the egg of GSP line. Interestingly, strong suppression to NDV was observed in the clone with N-Mx and in the eggs of N line. However, the effects of Mx for replication of vvMDV1 have not been. Thus, resistant types of cMx, N- and F-Mx, have showed the anti-viral effects to only RNA virus including NDV and VSV, but not to DNA virus. Antiviral effects of cMx were required whole length of amino acid including Val and Asn in amino acid 548 and 631.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.9
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• pp.1880-1889
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• 1997

CMS-MX(CDMA Mobile System-Mobile eXchange) software has a large-scale and needs frequency chanbes to adopt new functions. Therefore, an efficient version management is necessary for the software. General-purpose version management system are not suitable for the CMS-MX software, which consists of various types of files and has its own development method. In this paper, we present a version management system for the CMS-MX software. The version management system provides file registration control. version control, and information management on the basis of software configuration and soft ware developer information in accordance with CMS-MX software developement procement process. It has characteristics such as full file storing, file verification, and association with software integration process.

• Analytical Science and Technology
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• v.29 no.1
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• pp.29-34
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• 2016

This study explores the means by which MX can be effectively extracted from chlorinated water 3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), a potent mutagen commonly found in chlorinated drinking water at concentrations of up to a few hundred ng/L, was quantitatively determined using sample enrichment followed by liquid-liquid extraction (LLE), derivatization to methylated form, and analysis with GC-MS. A 4-L water sample was enriched to a concentration of 0.4 L using a vacuum rotary evaporator at 30 ℃. MX in the water was extracted using ethyl acetate (100 mL × 2) as a solvent and MX in the extract was methylated with 10 % H₂SO₄ in methanol. MX was recovered at a rate of 73.8 %, which was higher than that (38.1 %) for the resin adsorption method. The limit of quantification and repeatability (as relative standard deviation) were estimated to be 10 ng/L and 2.2 %, respectively. This result suggested that LLE can be used for the determination of MX in chlorinated water as an alternative to more time-consuming resin adsorption method.

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1088-1097
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• 2021

Grouper nervous necrosis virus (GNNV) infection causes mass grouper mortality, leading to substantial economic loss in Taiwan. Traditional methods of controlling GNNV infections involve the challenge of controlling disinfectant doses; low doses are ineffective, whereas high doses may cause environmental damage. Identifying potential methods to safely control GNNV infection to prevent viral outbreaks is essential. We engineered a virus-binding bacterium expressing a myxovirus resistance (Mx) protein on its surface for GNNV removal from phosphate-buffered saline (PBS), thus increasing the survival of grouper fin (GF-1) cells. We fused the grouper Mx protein (which recognizes and binds to the coat protein of GNNV) to the C-terminus of outer membrane lipoprotein A (lpp-Mx) and to the N-terminus of a bacterial autotransporter adhesin (Mx-AIDA); these constructs were expressed on the surfaces of Escherichia coli BL21 (BL21/lpp-Mx and BL21/Mx-AIDA). We examined bacterial surface expression capacity and GNNV binding activity through enzyme-linked immunosorbent assay; we also evaluated the GNNV removal efficacy of the bacteria and viral cytotoxicity after bacterial adsorption treatment. Although both constructs were successfully expressed, only BL21/lpp-Mx exhibited GNNV binding activity; BL21/lpp-Mx cells removed GNNV and protected GF-1 cells from GNNV infection more efficiently. Moreover, salinity affected the GNNV removal efficacy of BL21/lpp-Mx. Thus, our GNNV-binding bacterium is an efficient microparticle for removing GNNV from 10‰ brackish water and for preventing GNNV infection in groupers.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.38 no.6
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• pp.707-716
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• 2020

Spectroradiometers have recently been drawing great attention in earth observing communities for its capability for obtaining target's quantitative properties. In particular, light-weighted multispectral cameras are gaining popularity in many field domains, as being utilized on UAV's. Despite the importance of the radiometric accuracy, studies are scarce on the performance of the inexpensive multispectral camera sensors that have various applications in agricultural, vegetation, and water quality analysis. This study conducted assessment of radiometric accuracy for MicaSense RedEdge-MX multispectral camera, by comparing the radiometric data with an independent hyperspectral sensor having NIST-traceable calibration quality. The comaprison showed that radiance from RedEdge-MX is lower than that of TriOS RAMSES by 5 to 16% depending on the bands, and the irradiance from RedEdge-MX is also lower than RAMSES by 1~20%. The correction coefficients for RedEdge-MX alculated through the 1-st and the 3-rd order regression analysis were presented as a result of the study.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.7
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• pp.855-862
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• 2010

Among the known interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful pathways. The Mx protein has direct antiviral activity and inhibits a wide range of viruses by blocking an early stage of the viral replication cycle. Cloning, characterization, and expression of Mx in vivo and in vitro have been conducted. The chicken Mx gene spans 21 kb and is made up of 14 exons and 13 introns, of which the promoter region was analyzed. The real-time PCR results showed that Mx expression was increased in chicken embryo fibroblasts (CEF) after 12- and 24-h induction with polyI: C. Induction of Mx expression by poly I: C in vivo revealed tissue-specific patterns among the chicken tissues tested. A trace expression of Mx was detected in healthy chicken liver tissues from adult chickens without inducement; the expression levels in the liver, heart, and gizzard were higher than in the muscle and kidney. This is the first report to demonstrate the expression of a glutathione-S-transferase-tagged-Mx fusion protein of 75 KDa, as well as the biological activity tested by SDS-PAGE and western blotting.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2022.11a
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• pp.96-98
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• 2022

MX-S2X(선박중심직접통신)은 디지털 통신 연계기술 개발 및 실증을 통한 육상·선박·시설의 유기적 연결을 제공함으로써, 향후 도래할 해상 통신 기술 인프라로서의 효과적 활용이 가능할 것으로 기대된다. 본 논문은 '초고속해상무선통신망 무설설비 다각화 및 통신연계 기술개발 '의 1차년도에 수행한 MX-S2X 시스템의 물리계층 설계에 대한 보완 및 해상통신을 위한 최적화를 통해 상세설계를 확정하고, 이를 구현/제작한 HW의 성능을 검증한다. 제작한 HW의 검증을 위해 시험환경을 구성하여 PER 성능을 측정하고 M&S 결과와 비교 분석함으로써 MX-S2X HW의 기능 및 성능을 검증하였다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2021.11a
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• pp.121-123
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• 2021

선박중심직접통신(MX-S2X)은 디지털 통신 연계기술 개발 및 실증을 통한 육상·선박·시설의 유기적 연결을 제공함으로써, 해양사고 저감 및 자율운항선박을 위한 인프라로 활용될 수 있다. 본 논문은 MX-S2X 기술개발에 대한 소개, MX-S2X 개발을 위한 운용 및 설계 요구사항에 대해 기술하고 있으며, 더 나아가 해상 통신 환경이 통신성능에 영향을 끼칠수 있는 경우에 대해 살펴보고 이를 극복하기 위한 MX-S2X 시스템의 물리계층 설계에 대해 기술하였다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.11a
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• pp.149-151
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• 2023

MX-S2X(선박중심직접통신)은 선박을 중심으로 한 D2D 통신을 통해 육상·선박·시설의 유기적 연결을 제공함으로써, 해양 분야의 4차 산업혁명에서 해상 통신 기술 인프라로서의 핵심 역할이 가능할 것으로 기대 된다. '초고속해상무선통신망 무설설비 다각화 및 통신연계 기술개발 '의 1단계 3차년도에서는 2차년도에 제작한 MX-S2X 1차 시제품에 대한 기능을 보완하고 ISM 대역의 소출력 요구사항을 만족하기 위한 DSSS을 적용하여 2차 시제품을 구현/제작하고 실제 해상환경에서 성능을 검증한다. 본 논문에서는 MX-S2X 2차 시제품에 대한 1, 2차 실해역 시험결과 및 향후 계획에 대해 공유한다.