• Animal Bioscience
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• 제34권5호
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• pp.801-810
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• 2021

Objective: microRNAs (miRNAs) can play a role in a variety of physiological and pathological processes, and their role is achieved by regulating the expression of target genes. Our previous high-throughput sequencing found that ssc-miR-185 plays an important regulatory role in piglet diarrhea, but its specific target genes and functions in intestinal porcine epithelial cell (IPEC-J2) are still unclear. We intended to verify the target relationship between porcine miR-185 and cell division cycle 42 (CDC42) gene in IPEC-J2 and to explore the effect of miR-185 on the proliferation of IPEC-J2 cells. Methods: The TargetScan, miRDB, and miRanda software were used to predict the target genes of porcine miR-185, and CDC42 was selected as a candidate target gene. The CDC42-3' UTR-wild type (WT) and CDC42-3'UTR-mutant type (MUT) segments were successfully cloned into pmirGLO luciferase vector, and the luciferase activity was detected after co-transfection with miR-185 mimics and pmirGLO-CDC42-3'UTR. The expression level of CDC42 was analyzed using quantitative polymerase chain reaction and Western blot. The proliferation of IPEC-J2 was detected using cell counting kit-8 (CCK-8), methylthiazolyldiphenyl-tetrazolium bromide (MTT), and 5-ethynyl-2'-deoxyuridine (EdU) assays. Results: Double enzyme digestion and sequencing confirmed that CDC42-3'UTR-WT and CDC42-3'UTR-MUT were successfully cloned into pmirGLO luciferase reporter vector, and the luciferase activity was significantly reduced after co-transfection with miR-185 mimics and CDC42-3'UTR-WT. Further we found that the mRNA and protein expression level of CDC42 were down-regulated after transfection with miR-185 mimics, while the opposite trend was observed after transfection with miR-185 inhibitor (p<0.01). In addition, the CCK-8, MTT, and EdU results demonstrated that miR-185 promotes IPEC-J2 cells proliferation by targeting CDC42. Conclusion: These findings indicate that porcine miR-185 can directly target CDC42 and promote the proliferation of IPEC-J2 cells. However, the detailed regulatory mechanism of miR-185/CDC42 axis in piglets' resistance to diarrhea is yet to be elucidated in further investigation.

• 제어로봇시스템학회논문지
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• 제15권3호
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• pp.323-329
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• 2009

This paper presents an automatic LCD gamma control system using gamma curve optimization. It controls automatically gamma adjustment registers in mobile LCD driver IC to reduce gamma correction error and adjusting time. The proposed gamma system contains Module-Under-Test (MUT, LCD module), PC installed with program, multimedia display tester for measuring luminance, and control board for interface between PC and LCD module. Proposed algorithm and program are applicable for most of the LCD modules. It is realized to calibrate gamma values of 1.8, 2.0, 2.2 and 3.0. The control board is designed with DSP and FPGA, and it supports various interfaces such as RGB and CPU. Developed automatic gamma control system showed significantly reduced gamma adjusting time of 240 sec. and much less average gamma error of 11% than 42h and 27% with conventional manual method. We believe that the proposed system is very useful to provide high-quality LCD and to improve production process.

• 전기학회논문지
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• 제66권8호
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• pp.1215-1221
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• 2017

Parametric array transducers are used for highly directional speaker in an air environments. Piezoelectric micromachined ultrasonic transducers for parametric array transducers need DC-biased voltage driving signals in order to get high-directional quality-sound features. The existing power amplifier such as class A amplifiers has low efficiency and require large volume heatsinks. To overcome the above-mentioned disadvantages of the conventional amplifier, this paper proposes a new power amplifier system. The proposed power amplifier system ensures high linearity of output characteristic by utilizing the push-pull class B type amplifier. Furthermore, the proposed power amplifier system gets high efficiency because it contains the DC-DC converter-type power supply which can perform energy recovery and envelope tracking function. Also the paper suggests the detailed circuit topology. Its characteristics are verified by the detailed experimental results.

• 한국식품영양학회지
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• 제6권1호
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• pp.1-7
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• 1993

19 Samples of koji-starters using in brewing were collected from Korea and Japan, and then 31 strains of koji-molds were isolated from them. After Identification of the Isolate, rice koji was made with each strains, and its saccharogenic activity, dextrogenic activity, proteolytic activity, acid Producing ability, browning reaction and flavor were tested. Among 31 strains of isolates, 10 strains were Identified as Asp nwamori var. kawachii, 18 strains as Asp. oryzae, 3 strains as Asp. usamii mkt. shirousamii. The koji-starters made in Korea were composed of single species of koji-mold with same strain, but those made in Japan were composed of the mixture of different two species or the mixture of different 2 ∼4 strains in same species. Judging from amylolytic and proteolytic ability by species, Asp. awamori var. kawachii H1, I1 and 11, Asp. owsae J2, L2, M2, P3 and P4, and Asp. usamii writ. shirousamii S1 were better than the others. Mold strains isolated from Korean koji-starters were much lower in amylolytic or proteolytic activity than those from Japanese koji-starters. The typical characteristics for the 3 species of koji-molds were that Asp. awamori var. kawachii was strong in acid producing ability, but week in amylolytic and proteolytic ability, that Asp. owzae had strong amylolytic activity and good aroma, but produced little amount of acid, and that Asp. usamii mut. shirousamii had strong Proteolytic activity but some off-flavor.

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2004년도 학술논문집
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• pp.261-267
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• 2004

처분시스템 설계 시 기초 자료로 사용되는 국내 사용후핵연료의 발생량, 특징 및 연소이력 등의 현재 및 향후 현황을 파악하였다. 2055년까지 PWR 및 CANDU 사용후핵연료 발생량은 각각 20,500 및 14,800MTU로 나타났다.$17{\times}17$ 핵연료 집합체의 사용후핵연료 발생량비율은 2003년 기준으로 전체대비 60%를 점유하는 것으로 나타났으며, 2012년 이후부터는 .$16{\times}16$ KSFA 사용후핵연료 발생량이 .$17{\times}17$ 핵연료를 능가하기 시작하여 최종시점인 2055년에는 70% 정도를 점유할 것으로 보인다. 사용후핵연료의 평균 연소도는 90년대 후반에는 36GWD/MUT 정도, 2000년대 초반에는 40GWD/MTU를 나타냈으며, 2000년대 중ㆍ후반부터는 45GWD/MTU를 초과할 것으로 보인다. 따라서, 현재는 1997년에 선정한 제원을 기준 핵연료 제원으로 사용하되, 2010년을 기점으로 기준핵연료를 .$16{\times}16$ KSFA 4.5w/o, 55GWD/MTU로 반영하는 것이 타당해 보인다.

• 한국식품영양학회지
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• 제17권2호
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• pp.199-205
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• 2004

파쇄미, 색채불량미와 일반미에 효모 Saccharomyces cerevisiae, 혼합 밀 누룩으로 Aspergillus oryzae와 Aspergillus niger mut. kawachii를 접종하여 만든 개량누룩으로 탁주를 2단 담금한 후 발효과정 중의 품질을 검토한 결과는 다음과 같다. pH는 3.5 정도로서 잡균에 의한 오염이 방지되면서 곰팡이가 생성한 당화 효소에 의해 2일에 당화되었고, 환원당이 알코올 발효되면서 ethanol 18%로 정상적으로 알코올 발효가 진행되었다. 유기산 함량은 일반미에서 citric acid가 매우 높게 나왔다. 탁주 술덧에 함유하는 미량의 알코올로서 n-propanol, 2-methyl-1-propanol, 1-butanol, 3-methyl-1-butanol이 검출되었다. 일반미에서만 1-butanol이 정량되지 않았다. 술덧의 유리당은 파쇄미, 색채불량미에 비해 일반미에서 sucrose함량이 현저히 낮게 나왔다. 유리아미노산 함량은 일반미 술덧이 단맛을 나타내는 serine, glycine, alanine과 쓴맛을 나타내는 leucine, isoleucine, phenylalanine의 함량이 낮게 나왔으며, 색채불량미와 파쇄미술덧은 이와 반대의 결과를 나타내었다. 색차기에 의한 색의 구분에서 뚜렷한 차이가 없었고, 발효후 5단계 평점법에 의해 맛, 향, 색등을 관능검사한 결과 모두 4.3 이상의 $\ulcorner$좋다$\lrcorner$로 평가되어 전통탁주의 이용이 가능할 것으로 사료된다.

• The Plant Pathology Journal
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• 제36권5호
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• pp.440-449
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• 2020

The purpose of this study was to analyze the genetic and pathogenic characteristics of Ralstonia pseudosolanacearum in roses in Korea, and to examine the similarities and differences between Korean isolates and the first-reported European strains. Between 2017 and 2019, seventeen isolates from rose plants were identified as R. pseudosolanacearum using Ralstonia-specific primers. All 17 isolates were identified as race 1 using race-specific primers, and were confirmed as biovar 3 due to their ability to utilize carbon sources. Multiplex PCR using phylotype discriminating specific primers identified the 17 isolates as phylotype I. Sequevar comparison with reference sequevars using the sequences of the egl, mutS, and fliC genes, and only the egl gene, revealed that the strains evaluated in this study corresponded to sequevar I-33. The pathogenicity in roses differed depending on the rose cultivars. The different methods used for the genetic characterization of R. pseudosolanacearum indicate that the 17 rose bacterial wilt isolates had the same genetic characteristics. The lack of genetic variation in these isolates indicates their recent introduction from other countries (likely European countries). Therefore, appropriate quarantine and control measures should be taken in order to avoid further increases in the pathogenicity and/or secondary host range of R. pseudosolanacearum through genetic mutation.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1098-1105
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• 2017

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones ($Mut^s$) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6411-6413
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• 2013

Adenocarcinoma of esophagus (AE) is a complex disease, affected by a variety of genetic and environmental factors. Much evidence has shown that the MutY glycosylase homologue (MUTYH) plays a key role in the pathogenesis of many cancers. However, there have been no reports on influence on AE in the Han Chinese population. The objective of this study was to investigate this issue. A gene-based association study was conducted using three single nucleotide polymorphisms(SNPs) reported in previous studies. The three SNPs (rs3219463, rs3219472, rs3219489) were genotyped in 207 unrelated AE patients and 249 healthy controls in a case-control study using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The results revealed that the genotype distribution of rs3219472 differed between the case and control groups (OR=1.66,95%CI=1.11-2.48, P=0.012), indicating that an association may exist between MUTYH and AE. These findings support a signifcant role for MUTYH in AE pathogenesis in the Han Chinese population.

• BMB Reports
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• 제43권12호
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• pp.801-806
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• 2010

The existence of glycine residues in long-chain scorpion toxins has been well documented. However, their role as analgesics has not been evaluated. To address this issue, we investigated the functional role of glycines in the C-terminal end of Chinese-scorpion toxin from Buthus martensii Karsch (BmK AGP-SYPU2) using site-directed mutagenesis and analgesic activity assays. Recombinant BmK AGP-SYPU2 and its mutants were efficiently expressed in E. coli and purified to homogeneity using immobilized metal ion affinity chromatography (IMAC) and cation exchange chromatography. The mouse-twisting test was used to detect the analgesic activity of BmK AGP-SYPU2 and its mutants. As a result, we identified glycines at the C-terminal end that, when altered, significantly affected analgesic activity. Also, Mut6566 was significantly decreased compared to BmK AGP-SYPU2. These data indicate that the glycines at the C-terminal end are important for the analgesic activity of BmK AGP-SYPU2.