• 대한의용생체공학회:의공학회지
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• 제32권3호
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• pp.185-190
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• 2011

The purpose of this study is to investigate the influence of organism vigor information solution(OVIS) on the animal cell growth and to set up a condition for cell culture. We investigated the reactions on the MG-63 and MCF-7 cell line in mixture culture media with various amount of REVIEW solution, which is an example among various OVIS. DMEM-HG was used as basic media. The concentration range of the mixture was limited from 0% to 15%. MTT assay is used for cell viability test. The cell was incubated for 14days and the MTT assay was performed on day 1, 3, 7, 10 and 14 throughout the experiment. We used the ELISA reader to measure the Optical Density(O.D) at 595 nm wavelength filter. MCF-7 was linearly proliferated according to culture time and concentrations of OVIS. On the other hand, MG-63 cells were measured the highest O.D value at 12%. The growth rates of both cells cultured in mixed culture media with OVIS are much higher than those in only basic media, DMEMHG, after 14days. It was confirmed that cell cultured at OVIS grows rapidly at certain period although cells showed a negative effect in initial stage.

• 동아시아식생활학회지
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• 제4권2호
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• pp.59-67
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• 1994

The present study was conducted to evaluate the hepatoprotective effect of puerariae Radix methanol extract on benzo(a) pyrene(B(a)P) - induced liver injuries in rats. In vitro experiment, primary cultured hepatocytes (5X105 cells/$m\ell$) were cultured for 20~24 hours after adding puerariae Radix mehtanol extract(32$\mu\textrm{g}$/$m\ell$) and B(a)P(50 uM). In vivo experiment, Puerariae Radix methanol extract(0.25 g/kg/day, per os) was administered for 7 days and B(a)P(0.1 mg/kg/day, intraperitoneally) was given after the last administration of extract. And then the hepatoprotective effect of Puerariae Radix methanol extract was investigated biochemically through in vitro and in vivo experiments. Namely, activities of enzymes (GOT, GPT and LDH) were measured and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT) assay were carried out in vitro cell culture study and GOT, GPT, LDH and ALP activities and HDL-cholesterol, total cholesterol and triglyceride contents were performed in vivo study. In vitro experiment, as a result of enzyme activity measurement(GOT, GPT and LDH) and MTT assay, GOT,GPT and LDH activities changed by B(a)P were recovered to normal levels and hepatocytes impaired by B(a)P were recovered to normal. In vivo experiment, Puerariae Radix methanol extract significantly decreased the enzyme activities(GOT, GPT, ALP and LDH in serum and GPT and ALP in tissue) and lipid contents in comparison to B(a)P-treated group.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.54-60
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• 2003

To develop a functional chungkookjang; the anticancer effects of chungkookjangs prepared with different varieties of soybeans, starters, fermentation periods and seasoning additive ratios; were studied against AGS human gastric adenocarcinoma cells using the MTT assay, at different stages chungkookjang processing. The chungkookjang samples exerted different antiproliferative effects according to the variety of soybeans used. The chungkookjangs manufactured with soybean var. manrikong exhibited the highest cytotoxicity against AGS human cancer cells. The chungkookjangs fermented with rice straw and B. licheniformis strongly inhibited the growth of the AGS human cancer cells. All fermented chungkookjangs had a strong inhibitory effect on the growth of the cancer cells; however, the non-fermented soybean (chungkookjang) showed a low inhibition rate. The fermented chungkookjangs mixed with red pepper powder (RPP) and garlic exhibited strong antiproliferative effect against the cancer cells, and chungkookjang prepaved with 1.1 % RPP and 1.1 % garlic showed the highest cytotoxicity against the cancer cells. The functional chungkookjang fermented with soybean variety of manrikong and B. licheniformis for 3 days at 4$0^{\circ}C$ and then mixed with 7.9% salt, 1.l% RPP and 1.1% garlic, exhibited a higher antiproliferative effect than the chungkookjangs prepared by traditional or modified methods, according to the MTT assay. The functional chungkookjang exhibited a similar anticancer effect to the traditional doenjang. These results indicate that the fermentation period and the ratio of seasoning additives, as well as the variety of soybeans and starter cultures may affect the degree of the anticancer effect of chungkookjang.

• 한국축산식품학회지
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• 제27권4호
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• pp.538-542
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• 2007

본 연구는 kefir로부터 EPS를 분리하여 MA104 세포에 대한 독성과 rotavirus에 대한 저해효과를 확인하기 위하여 수행하였다. Kefir culture 및 grain 파쇄입자에서 Lactobacillus fermentum, Lactobacillus acidophilus, Lactobacillus brevis 등의 유산균과 Candida kefyr, Cryptococcus albidus, Pichia ohmeri 등의 효모가 분리 동정되었다. EPS의 1% 농도에서, MTT assay에 의한 EPS의 rotavirus에 대한 억제효과는 human rotavirus(KU)가 $72.52{\pm}6.48%$, bovine rotavirus(NCDV)가 $36.06{\pm}7.63%$, porcine rotavirus(OSU)가 $81.66{\pm}1.11%$로 나타났으며, EPS의 1/128% 농도에서, human rotavirus(KU)가 $24.98{\pm}4.58%$, bovine rotavirus(NCDV)가 $4.71{\pm}6.16%$, porcine rotavirus(OSU)가 $4.05{\pm}14.90%$로 나타났다. Kefir에서 분리한 EPS는 다양한 혈청형과 유래 동물의 rotavirus 모두에게 억제 효과가 있는 것으로 확인되었다.

• 대한본초학회지
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• 제32권6호
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• pp.41-48
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• 2017

Objectives : This study was designed to estimate the antioxidative and neuroprotective effects of Torreyae Semen hot water extracts (TS). Methods : Torreyae Semen was extracted by hot water for 2 hours with a temperature of 105 degrees. Polyphenols and total flavonoid were measured and LC-MS/MS was used to certificate anticipated antioxidative compounds. The antioxidant activities of TS were measured as scavenging effects of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and Nitrite Oxides (NO). Cell viability and proliferation rate was measured MTT assay. The toxicities to thymocytes and splenocytes were evaluated by the proliferation rate of primary cultured cells of 7 weeks, male Balb/c mice. The antioxidant activities of TS on C6 mouse glioma cells were measured by the analysis of total glutathione contents variation. The neuroprotective effects against oxidative stresses were measured by MTT assay. Results : Polyphenols of TS was $92.00{\pm}1.24{\mu}g/mg$, and total flavonoids was $0.36{\pm}0.14{\mu}g/mg$. TS includes gallocatechin, epigallocatechin, gallocatechin gallate and epigallocatechin gallate. TS included gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate. TS showed DPPH and NO scavenging effects as dose-dependent manner at the concentrations of $0-10mg/m{\ell}$. In MTT assay, TS shows no significant toxicity to C6 cells, primary cultured thymocytes and splenocytes of Balb/c mice. TS increased the level of total glutathiones. TS increased cell viabilities of C6 cells against oxidative stresses such as $H_2O_2$, sodium nitroprusside (SNP), Rotenone at the concentrations of $0-0.063mg/m{\ell}$. Conclusions : TS shows the antioxidant and neuroprotecitive effects in these experiments.

• 한국표면공학회지
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• 제56권4호
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• pp.273-282
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• 2023

In this study, the surface of Polyetheretherketone (PEEK) disks was modified to have a hydrophilic surface by applying a coating of Polyethylene glycol (PEG), Hyaluronic acid(HA), and Poly-Dopamine(PDA). The investigation aimed to examine whether the coated surfaces showed enhanced bioactivity for orthopedic applications compared to the pure PEEK. The microstructure, surface characteristics, and wettability of PEEK coated with PEG, HA, and PDA were analyzed using scanning electron microscopy(SEM), FT-IR spectrophotometer, Roughness Measurement System, Micro-Vickers, and Contact angle measurement. The mechanical properties were analyzed using a tensile testing machine, while the MTT assay for cell activity was analyzed using a microplate reader to measure optical density. According to the SEM and FT-IR results, the composition and crystal structure of PEG, HA and PDA coated surface were verified. Also, roughness, hardness, and contact angle were all improved in the coating group compared to the pure PEEK. We checked the HepG2 cell proliferation by using MTT assay on 7th days. In MTT assay results, HepG2 cell proliferation was increased with time, at 7 days, cell viability on discs coated with PDA was significantly higher than pure PEEK, PEG, HA coated group. PDA coated PEEK exhibited the highest surface roughness, hardness, contact angle, and cell activity. The mechanical properties were not affected by the presence of the coating.

• 한국자원식물학회지
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• 제26권1호
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• pp.111-118
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• 2013

본 연구에서는 붉은장목수수(Sorglum Bicolor M.) MeOH 추출물과 분획물들의 다양한 생리활성을 비교하기 위하여 항산화(DPPH assay, 환원력, 총 페놀 및 플라보노이드 함량), 항당뇨(${\alpha}$-Glucosidase 저해활성 및 ${\alpha}$-Amlyase 저해활성), 항암(MTT assay) 활성을 관찰 하였다. 항산화활성의 경우 EtOAc fraction이 높은 총 페놀과 플라보노이드 함량을 나타내었으며, DPPH assay와 reducing power 결과 역시 EtOAc fraction과 MeOH fraction이 positive control로 사용한 ${\alpha}$-tocopherol보다 우수한 활성을 나타냈다. ${\alpha}$-Glucosidase, ${\alpha}$-amlyase 저해능 평가 결과 D.W. fraction이 가장 높은 활성을 보였으며 n-BuOH fraction과 MeOH extract도 활성을 나타내었다. 암세포인 AGS, HT29, HCT116세포주에 대한 세포독성 연구인 MTT assay에서는 EtOAc, n-BuOH, D.W. fraction을 처리한 처리구에서 독성을 보였으며 이중 n-BuOH fraction이 모든 세포주에서 농도 의존적으로 세포독성을 가지는 것을 확인할 수 있었다. 연구결과 붉은장목수수의 추출물 EtOAc fraction의 항산화활성, D.W. fraction의 항당뇨활성, n-BuOH fraction의 암세포에 대한 독성과 관련된 화합물의 분리 및 구조규명에 관한 연구가 필요 할 것으로 사료되어지며, normal cell에 대한 MTT assay를 진행하여 항암활성에 관한 연구를 진행하여 붉은장목수수 추출물을 이용한 다양한 건강기능식품과 의약품 개발을 통해 건강 증진과 질병으로부터 보호 받을 수 있을 것으로 기대한다.

• 한국식품과학회지
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• 제50권1호
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• pp.1-7
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• 2018

본 연구에서는 식품성분이나 천연물의 세포활성 측정에 널리 사용되는 MTT 분석 상의 최종산물인 MTT 포마잔 색소에 대한 여러 용매 상에서의 안정성과 다양한 빛과 ZnPP가 미치는 영향을 조사하였다. MTT 포마잔의 발색도는 0.01 N 이상의 NaOH 농도에서 가장 높게 나타났으며, 증류수, DMSO, 에탄올, 메탄올 등의 용매에 비해 2배 이상의 발색강도를 보였다. 증류수나 0.01 N 이하의 NaOH 농도에서 MTT 포마잔은 불안정하여 빛의 조사와 관계없이 신속하게 분해되었으며 0.1 N NaOH, DMSO에서는 안정하였다. DMSO나 0.1 N NaOH 상에서 MTT 포마잔은 형광등과 UVA (365 nm)의 조사에 의해 2시간 내에 발색도의 현저한 변화가 관찰되지 않았으나, UVB (254 nm)에 노출되었을 경우 용매와 관계없이 신속하게 탈색되었다. MTT 포마잔은 에탄올, 메탄올, DMSO와 NaOH 용매상에서 ZnPP에 의해 시간과 농도에 의존적으로 분해되었으며, 0.1 N NaOH 상에서 가장 민감하고 신속한 탈색 양상을 나타내었다. DMSO와 0.1 N NaOH 상에서 MTT 포마잔은 $0.1{\mu}M$의 ZnPP에 의해서도 유의적인 탈색현상을 나타내었다. 하지만 ZnPP에 의한 MTT 포마잔의 탈색현상은 암소에서는 일어나지 않았으며, 빛과 관계없이 명소와 암소에서 모두 수용성 XTT 포마잔에는 미미한 효과를 나타내었다.

• 한국응용과학기술학회지
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• 제35권2호
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• pp.325-335
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• 2018

본 연구는 화장품 소재로서 인삼복합물의 가능성을 확인하기 위한 것이다. 이를 위해 우리는 인삼복합물 에탄올 추출물을 사용하여 항염증, 미백 효과에 대한 생물학적 활성 평가를 수행하였다. 시료는 인삼(A), 상황(B), 리기다소나무(C)를 각 70% 에탄올로 추출한 후 (A)1 : (B)1 : (C)0.5의 비율로 혼합한 시료를 사용하였다. 대식세포(RAW 264.7 cells)내에서 시료의 항염증 효과를 평가하기 위해 MTT assay를 이용한 샘플의 독성평가와 nitric oxide 생성 저해 활성 및 염증관련 단백질 및 유전자의 발현량을 확인하였다. 시료의 미백 효과를 평가하기 위해서 멜라노마(B16F10 cell)를 이용해 MTT assay를 통한 샘플의 독성평가, cellular tyrosinase 저해율 측정 및 멜라닌 생합성량을 측정하였다. LPS로 유도된 RAW 264.7 세포내에서 시료의 nitric oxide 저해 활성 결과는 $25{\mu}g/mL$ 농도에서 71.2%의 우수한 효능을 나타내었으며 western blot 실험결과 iNOS, COX-2 단백질의 발현은 농도 의존적으로 감소하는 것을 확인하였다. Tyrosinase 활성 억제 실험에서 인삼복합물의 농도 $50{\mu}g/mL$에서 36.8%의 감소를 나타냈으며 멜라닌 생합성 억제 평가 결과에서 농도 $50{\mu}g/mL$에서 47.8%의 감소를 나타냈다. 상기 실험 결과로부터 인삼복합물의 우수한 항염증, 미백 효능을 확인하였으며 향후 안전한 천연 화장품 원료로 사용될 수 있음을 확인하였다.

• Journal of the Korean Wood Science and Technology
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• 제37권1호
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• pp.78-86
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• 2009

본 연구에서는 느티나무의 에탄올 추출액에서 7-hydroxy-3-methoxycadalene 외에 3,7-dimethoxycadalene와 7-hydroxycadalene-5,8-quinone (keyakinone A) 등 2종의 카달렌 동족체를 분리하였고, 이 중에서 7-hydroxy-3-methoxycadalene을 대상으로 hydroxyl 라디칼 소거활성과 MTT assay에 의한 세포독성을 평가하였다. 7-Hydroxy-3-methoxycadalene의 hydroxyl 라디칼 소거활성은 농도에 의존적이며, 100 ppm에서는 hydroxy 라디칼이 거의 100% 제거되는 우수한 효과를 나타냈다. 그러나 7-hydroxy-3-methoxycadalene은 낮은 농도(1 ppm 이하)에서는 세포 생존율이 약 90% 가량으로 세포독성이 거의 나타나지 않았지만 농도가 높아질수록 세포 생존율에 크게 영향을 미치는 것으로 나타났다. 이러한 7-hydroxy-3-methoxycadalene은 4종의 느릅나무과 수종(느릅나무(Ulmus davidiana), 참느릅나무(Ulmus parvifolia), 큰잎느릅나무(Ulmus crophylla), 당느릅나무(Ulmus macrocarpa))에서는 전혀 발견되지 않았다. 느릅나무과 수종에서는 공통적으로 7-hydroxycadalene (분자량 214)으로 추정되는 화합물이 발견되었으며, 이는 느티나무에서 단리한 7-hydroxy-3-methoxycadalene에서 3번 위치의 메톡실기($OCH_3$)가 이탈된 구조의 화합물이다.