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Study on Cadalene Compounds Purified from Zelkova serrata Wood II - Biological activities of 7-hydroxy-3-methoxycadalene and purification of cadalene homologues -  

Choi, Joon-Weon (Dept. Forest Science, CALS, Seoul National University)
Mun, Sung-Hee (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Choi, Don-Ha (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute)
Publication Information
Journal of the Korean Wood Science and Technology / v.37, no.1, 2009 , pp. 78-86 More about this Journal
Abstract
In this study 2 cadalene homologues - 3,7-dimethoxycadalene and 7-hydroxycadalene-5,8-quinone (keyakinone A)-were further identified from ethanol extracts of Zelkova serrata wood, except 7-hydroxy-3-methoxycadalene. Two biological activities-scavenging activity of hydroxy radical and cell toxicity by MTT assay-were measured with 7-hydroxy-3-methoxycadalene. The scavenging activity of hydroxyl radical of the compound was excellent and increased with its concentration. At 100 ppm hydroxyl radicals were removed completely. However, MTT assay revealed that 7-hydroxy-3-methoxycadalene showed critical toxicity to the cells. When 1 ppm of the compound was treated to the cells, cell viability was reached up to 90%, while it was reduced to 22% after treatment of 9 ppm. In 4 different Ulmaceae species, such as Ulmus davidiana, Ulmus parvifolia, Ulmus macrocarpa, Ulmus macrophylla, 7-hydroxy-3-methoxycadalene was not found at all. Instead, 7-hydroxycadalene (Mw 214), in which methoxyl group is omitted from 7-hydroxy-3-methoxycadalene, was distributed in the heartwood of 4 Ulmaceae species as major cadalene compound.
Keywords
Ulmus davidiana; Ulmus parvifolia; Ulmus macrocarpa; Ulmus macrophylla 7-hydroxy-3-methoxycadalene; 3,7-dimethoxycadalene; 7-hydroxycadalene-5; 8-quinone; 7-hydroxycadalene; hydroxy radical; cell toxicity; MTT assay;
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