• Title/Summary/Keyword: MT3D

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Investigation of the efficacy of mycotoxin-detoxifying additive on health and growth of newly-weaned pigs under deoxynivalenol challenges

  • Holanda, Debora Muratori;Kim, Sung Woo
    • Animal Bioscience
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    • v.34 no.3_spc
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    • pp.405-416
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    • 2021
  • Objective: This study evaluated the effects of feeding diets naturally contaminated with deoxynivalenol (supplemental 2 mg/kg) on health, growth, and the effects of a mycotoxin-detoxifying additive in newly-weaned pigs. Methods: Thirty-six pigs (27 day-old) were housed individually and assigned to 3 treatments for 5 weeks: CON (diet containing minimal deoxynivalenol), MT (diet with supplemental 1.9 mg/kg of deoxynivalenol), and MT+D (MT + mycotoxin-detoxifying additive, 0.2%, MegaFix, ICC, São Paulo, Brazil). The mycotoxin-detoxifying additive included bentonite, algae, enzymes, and yeast. Blood was taken at week 2 and 5. Jejunal tissue were taken at week 5. Data were analyzed using the MIXED procedure of SAS. Results: Pigs fed MT+D tended to have decreased (p = 0.056) averaged daily feed intake during week 1 than MT. At week 2, serum aspartate aminotransferase/alanine aminotransferase in MT tended to be lower (p = 0.059) than CON, whereas it was increased (p<0.05) for MT+D than MT, indicating hepatic damages in MT and recovery in MT+D. Pigs fed MT had lower (p<0.05) blood urea nitrogen/creatinine than CON, supporting hepatic damage. At week 5, pigs fed MT tended to have reduced (p = 0.079) glucose than CON, whereas it was increased (p<0.05) for MT+D than MT, indicating impaired intestinal glucose absorption in MT, which was improved in MT+D. Pigs fed CON tended to have increased (p = 0.057) total glutathione in jejunum than MT, indicating oxidative stress in MT. Pigs fed MT+D had a reduced (p<0.05) proportion of Ki-67-positive cells in jejunum than MT, indicating lower enterocyte proliferation in MT+D. Conclusion: Feeding supplemental 1.9 mg/kg of deoxynivalenol reduced growth and debilitated hepatic health of pigs, as seen in leakage of hepatic enzymes, impaired nitrogen metabolism, and increase in oxidative stress. The mycotoxin-detoxifying enhanced hepatic health and glucose levels, and attenuated gut damage in pigs fed deoxynivalenol contaminated diets.

Cellular Slime Molds in Forest Soils of Central Areas of Korea (중남부 삼림 지역에서의 세포성 점균의 출현과 분포)

  • 박미아;장남기
    • Asian Journal of Turfgrass Science
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    • v.10 no.3
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    • pp.213-230
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    • 1996
  • A study of occurrence and distribution of cellular slime molds(CSMs) in forest soils of central areas of Korea was carried out. Samples for CSMs isolation were collected from 4 study sites; Mt.Kyeryong, Mt. Taebaek, Mt. Sobaek and Mt. Sokri. In Mt. Kyeryong, six species were found. These were Dictyostelium fasciculatum, D.firmibasis, D. mucoroides, D. minutum, D. brefeldianum and Polysphondylium pallidum. The average number of species isolated at one site was 0.75, and average density(clones /g soil) was 292. The results of soil sample analyses were that the concentration of Pb was higher than any other areas. In Mt. Taebaek, seven species were found; D. fasciculatum, D.firmibasis, D. mucoroides, D. miuutum, P.pallidum. P. violaceum, P. candidum. The average number of species isolated at one site was 2.3,and average density was 1,108. Based on importance values calculated from study sites within each of three elevation ranges, several of the more widely distributed and abundant species have distribution patterns that show a response to elevation. In Mt. Sobaek, eleven species were found. These were D. fasciculatum. D.firmibasis, D. mucoroides, D. miuutum, D. brefeldianum, D. crassicaule,D. deminutivum, D. implicatum, P. pallidum. P. violaceum and P. candidum. The average number of species isolated at one site was 3, and average density was 793. Species diversity appeared to be the highest in this area. In Mt. Sokri, six species were found. These were D. fasciculatum. D. mucoroides, D. minutum, D. purpureum, P. pallidum. and P. violaceum. The average number of species isolated at one site was 2.4 and average density was 858. It was noticeable that D.purpureum were much more comrnonly found in this pinus evergreen forest. Key words: Cellular slime molds(CSMs), Importance value, Elevation, Average number of species, Average density.

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A Study on Geoelectrical Structure of Jeju Island Using 3D MT Inversion of 2D Profile Data (2차원 MT 자료의 3차원 역산을 통한 제주도 지전기구조 연구)

  • Choi, Ji-Hyang;Kim, Hee-Joon;Nam, Myung-Jin;Lee, Tae-Jong;Han, Nu-Ree;Lee, Seong-Kon;Song, Yoon-Ho;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.10 no.4
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    • pp.268-274
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    • 2007
  • Traditional two-dimensional (2D) interpretation of magnetotelluric (MT) data utilizes only transverse magnetic (TM)-mode data, because 2D inversion of transverse electric (TE)-mode data results in spurious features when 3D structures exist in the subsurface. The application of a 3D inversion algorithm to a single MT profile can reduce contamination due to off-profile anomalies and help us to incorporate TE-mode data in the interpretation. In this study, we conduct 2D and 3D inversions of MT data observed along two lines in Jeju Island. First, we invert apparent resistivities and phases in the TM and TE modes separately. Then, we perform 2D joint inversion of both TM- and TE-mode data and 3D inversion of both Zxy- and Zyx-mode data corresponding to TE- and TM-mode data in 2D. The resistivity images derived from all four data show that the geoelectrical structure in Jeju Island is a three-layered earth with the resistive-conductive-resistive stratigraphy within a depth of 5 km. The 3D inversion does not produce clear anomalies in the reconstructed profile image, while all of 2D do. This attributed to the possibility that 2D inversion results are distorted by exiting off-profile 3D anomalies in Jeju. With 3D inversion of 2D profile MT data, we can deduce more reliable results that are not seriously distorted by off-profile 3D anomalies.

Correction of the Sea Effect in the Magnetotelluric (MT) Data Using an Iterative Tensor Stripping During Inversion (MT 자료 역산과정에서 반복적인 Tensor Stripping을 통한 해양효과 보정)

  • Yang, Jun-Mo;Lee, Chun-Ki;Yoo, Hai-Soo
    • Geophysics and Geophysical Exploration
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    • v.11 no.4
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    • pp.286-301
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    • 2008
  • When magnetotelluric (MT) data are obtained in vicinity of the coast, the sea can distort observed MT responses, especially those of deep part of subsurface. We introduce an iterative method to correct the sea effect, based on the previous topographic correction method which removes the distortions due to topographic changes in seafloor MT data. The method first corrects the sea effect in observed MT impedance, and then inverts corrected responses in a model space without the sea. Due to mutual coupling between sea and subsurface structure, the correction and inversion steps are iterated until changes in each result become negligible. The method is validated for 1-D and 2-D structure using synthetic MT data produced by 3-D forward modeling including surrounding seas. In all cases, the method closely recovers the given structure after a few iterations. To test the applicability of the proposed method to field data, we generate synthetic MT data for the Jeju Island whose 1-D conductivity structure is well known, using 3-D forward modeling. The distortions due to the surrounding sea start to appear below the frequency about 1 Hz, and are relatively severe in the electrical field perpendicular to the coastline because of the location of the observation sites. The proposed method successfully eliminates the sea effect after three iterations, and both 1-D and 2-D inversion of corrected responses closely recover the given subsurface structure of the Jeju Island model.

In Vitro Propagation of Zanthoxylum piperitum DC. - II. Effect of $NH_4NO_3, KNO_3$ and Casein hydrolysate on Somatic Embryogenesis- (초피나무 (Zanthoxylum piperitum DC) 의 기내증식 - II. $NH_4NO_3, KNO_3$ Casein hydrolysate의 기내 부정배 발생효과 - ( In Vitro Propagation of Zanthoxylum piperitum DC. - II. Effect of NH4NO3 , KNO3 and Casein hydrolysate on Somatic Embryogenesis - ))

  • 송원섭
    • Korean Journal of Plant Resources
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    • v.8 no.2
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    • pp.153-157
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    • 1995
  • Embryogenic callus induces from shoot tip and leaf segment of Zanthoxylum piperitum for producing somatic embryogenesis and plant regeneration were cultured in vitro on Murashige and Tucker's(MT) medium treated with casein hydrolysate $NH_4NO_3$, $KNO_3$ and plant growth regulator. The most effective somatic embryogensis was observed in the medium added by two fold $NH_4NO_3$(3300mg/l)+2. 4-D 0.1mg/l and $KNO_3$(3800mg/l)+2.4-D 0.1mg/l. Also, MT medium supplemented with casein hydrolysate 700mg/l added by 2, 4-D 0.1mg/l were effective in obtainingn somatic embryos from embryogenic callus The effect ofm MT medium supplemented with casein hydrolysate without 2, 4-D was lower than that with (3300mg/l) 2, 4-D for the formation of somatic embryos.

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MT Response of a Small Island Model with Deep Sea and Topography (깊은 바다와 지형을 고려한 소규모 섬 모델의 MT 반응 연구)

  • Kiyeon Kim;Seong Kon Lee;Seokhoon Oh;Chang Woo Kwon
    • Geophysics and Geophysical Exploration
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    • v.27 no.1
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    • pp.37-50
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    • 2024
  • The magnetotelluric (MT) survey can be affected by external environmental factors. In particular, when acquiring MT data in islands, it is essential to consider the combined effect of topography and sea to understand the results and make accurate interpretations. To analyze the MT response (apparent resistivity, phase) with consideration of the effect of topography and sea, a small cone-shaped island model surrounded by deep sea was created. Two-dimensional (2-D) and three-dimensional (3-D) forward modeling were performed on the terrain model considering topography and the island model considering both topography and sea. The 2-D MT response did not reflect the topographic and sea effect of the direction orthogonal to the 2-D profile. The 3-D MT response included topographic and sea effects in all directions. The XY and YX components of the apparent resistivity were separated on undulating topography, such as a hill. A conductor at 1 km below sea level could be distinguished from topographic and sea effects in the MT response, and low resistivity anomaly was attenuated at greater depths. This study will facilitate understanding of field data measured on small islands.

Application of Two-Dimensional Boundary Condition to Three-Dimensional Magnetotelluric Modeling (3차원 MT 탐사 모델링에서 2차원 경계조건의 적용)

  • Han, Nu-Ree;Nam, Myung-Jin;Kim, Hee-Joon;Lee, Tae-Jong;Song, Yoon-Ho;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.11 no.4
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    • pp.318-325
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    • 2008
  • Assigning an exact boundary condition is of great importance in three-dimensional (3D) magnetotelluric (MT) modeling, in which no source is considered in a computing domain. This paper presents a 3D MT modeling algorithm utilizing a Dirichlet condition for a 2D host. To compute boundary values for a model with a 2D host, we need to conduct additional 2D MT modeling. The 2D modeling consists of transverse magnetic and electric modes, which are determined from the relationship between the polarization of plane wave and the strike direction of the 2D structure. Since the 3D MT modeling algorithm solves Maxwell's equations for electric fields using the finite difference method with a staggered grid that defines electric fields along cell edges, electric fields are calculated at the same place in the 2D modeling. The algorithm developed in this study can produce reliable MT responses for a 3D model with a 2D host.

Effects of 3D Topography on Magnetotelluric Responses (MT 탐사의 3차원 지형효과)

  • Nam, Myung-Jin;Kim, Hee-Joon;Song, Yoon-Ho;Lee, Tae-Jong;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.10 no.4
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    • pp.275-284
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    • 2007
  • For precise interpretation of magnetotelluric (MT) data distorted by irregular surface terrain, topography effects are investigated by computing apparent resistivities, phases, tippers and induction vectors for a three-dimensional (3D) hill-and-valley model. To compute MT responses for the 3D surface topography model, we use a 3D MT modeling algorithm based on an edge finite-element method which is free from vector parasites. Distortions on the apparent resistivity and phase are mainly caused by distorted currents that flow along surface topography. The distribution of tipper amplitudes over both hill and valley are the same, while the tipper points toward the center of hill and the base of the valley. The real part of induction vector also points in the same direction as that of tipper, while the imaginary part in the opposite direction.

Mitochondrial DNA polymorphism in Fourteen Geographical Strains of Drosophila melanogoater (세계 14지역 계통에 대한 초파리 미토콘드리아 DNA의 다형현상)

  • 김봉기
    • The Korean Journal of Zoology
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    • v.31 no.3
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    • pp.218-224
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    • 1988
  • Restriction endonucleases were used to search for intraspecific variation at 32 cleavage sites in mitochondrial DNA(mtDNA) purified from fourteen strains of Drosophila melanogaster helonging to different localities of the world. mtDNA of D. melanogaster was displayed site variation(Hpall, Haelll and Seal endonucleases) and length variation(maxirnum 550bp). Six genotypes, Ml, M2, M3, M4, M6 and M7, could be distinguished based on ihe site types witti a low average of intraspecific substitution rate (1.88%),but M5 type of Ogasawara strain in Japan was not detected in this study. A possible explanation for the low divergence was that mtDNA variation of fourteen strains in D. melanogaster could not he accumulated sufficiently owing to recent divergence of few individuals, and that sequence divergence was prevented by frequent migration in spite of the geographical isolation.

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Analysis of MT Data Acquired in Victoria, Australia (호주 Victoria주 MT 탐사 자료 해석)

  • Lee, Seong-Kon;Lee, Tae-Jong;Uchida, Toshihiro;Park, In-Hwa;Song, Yoon-Ho;Cull, Jim
    • Geophysics and Geophysical Exploration
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    • v.11 no.3
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    • pp.184-196
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    • 2008
  • We perform MT soundings in Bendigo, the northern part of Victoria, Australia, to investigate the deep subsurface geologic structure. The primary purpose of this survey is to figure out whether the discontinuity such as faults extends northward. The time series of MT signal were measured over 11 days at 71 measurement stations together with at remote reference, which help enhance the quality of impedance estimation and its interpretation. The impedances are estimated by robust processing using remote reference technique and then inverted with 2D MT 2D inversion. We can see that known faults are clearly imaged in MT 2D inversion. Comparing resistivity images from MT 2D inversion with interpreted boundary from reflection seismic exploration, two interpretations match well each other.