• Title/Summary/Keyword: MT1M

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Expression of Membrane-Type Matrix Metalloproteinase 1 and 2 in Mouse Oocytes, Embryos, Ovary and Oviduct (생쥐 난자와 배아 및 난소와 수란관의 Membrane-Type Matrix Metalloproteinase 1 및 2의 유전자 발현)

  • 김지영;이희진;김소라;김해권;강성구;이승재;조동제
    • Development and Reproduction
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    • v.4 no.1
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    • pp.45-52
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    • 2000
  • Membrane type matrix metalloproteinases(MT-MMPs) have been suggested to play an important role during structural remodeling of various tissue. Expression patterns of MT1-MMP and MT2-MMP mRNAs were investigated in oocytes, embryos, ovary and oviduct of mouse during their differentiation or periovulatory period using RT-PCR technique. Both cDNA products of MT1- and MT2-MMP of immature oocytes were barely discernable with a minimum amount but the expressions were distinct in mature oocytes regardless that they were matured in vivo or in vitro. MT2-MMP was not expressed by 2-cell embryos but was expressed by 4-cell stage embryos. From the morula stage untill hatched blastocyst stage, embyos showed intesnse expression of MT2-MMP with a sudden increase at blastocyst stage. While mouse ovarian tissues showed both expression of MT1- and MT2-MMP, there was no stage-specific difference throughout the estrous cycle. Mouse oviducts also exhibit constant amount of both MT1- and MT2-MMP expressions throughout periovulatory period, i.e., before or after ovulation. These observations lead to suggest that the differential expressions of maternal MT1- and MT2-MMP during meiotic resumption of mouse oocytes and embryonic expression of MT2-MMP particularly at blastocyst stage might play a role in the differentiation of mouse oocytes and/or embryos. The precise function of MT1- and MT2-MMP with regards to their participation in the remodeling of ovarian and oviductal tissues remains in a question.

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A Comparative Study on the Habitat of Abies koreana WILSON between Mt. Jiri and Mt. Ha1la

  • Ihm, Byung-Sun;Lee, Jeom-Sook;Kim, Moon-Hong;Kim, Ha-Song
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2000.10b
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    • pp.60-64
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    • 2000
  • This examination of vegetation is conducted from lune to Oct. 1999 through on the spot survey, centering on the habitat of Abies koreana around Imgeolryeong, langteomok, and Jeseokbong in Mt Jiri and Yeongsil path and Seongpanak path in Mt. Halla. Species composition tables of the Abies koreana community by altitude and slope in Mt. Jiri and Mt. Halla are made based on the community composition tables examined in quadrat. The Abies koreana-Saso quelpaertensis community in a west slope of Mt. Halla is found that Abies koreana of 4-6m in height forms subtree layer and that of 8-9m in height the tree layer and it reflects a difference between community structure by slope and main composition species. While the tree layer of Abies koreana community is 12-14 in height around the area of 1, 290-1, 560m above the sea of Imgeolryong and path from Baekmudong to Jangteomok, it is 8-12m in height in the area of 1, 680-1, 780m above the sea of path from Jangteomok to Jeseokbong. It means that community structure depends on area and attitude. This study finds out that commonly appearing composition species similar to coverage and presence are Lepisorus thunberianus, Quercus mongolica, Soidago virga-aurea var. asiatica, Ligularia fischeri, Carex lanceolata, Clintonia udensis, Magnolia sieboldii, Betula ermani, Acer Pseudo-sieboldianum, As soil environment of surveyed area, pH of surveyed spots is similar in Mt. Jiri and Mt. Halla, electrical conductvity is higher in Mt Jiri, and contents of organic matter is relatively higher in a west slope of Mt. Jiri and Mt. Halla and lower in their south and west slopes. Contents of organic matter and total nitrogen show that the area of 1, 550m above the sea in a west slope of Mt. Halla is some higher. For pH and contents of total nitrogen and organic matter, Mt. Halla is higher than Mt. Jiri but for electrical conductivity , Mt. Jiri is relatively higher than Mt. Halla.

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Analysis of Benthic Macroinvertebrate Community and Biological Evaluation of Water Quality in the Gapyung Region of the Upper North Han River, Korea (한강 상류수계(가평)의 저서성 대형무척추동물 군집분석 및 생물학적 수질평가)

  • 배경석;유승성;원두희;김민영;신재영
    • Journal of Environmental Health Sciences
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    • v.28 no.2
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    • pp.149-160
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    • 2002
  • Analysis of benthic macroinvertebrate community structure and biological estimation of water quality by the benthic macroinvertebrates from the Mt. Myungji(1267m), Mt. Kalbong(840m), Mt. Daegeum(704m) and Mt. Jubal(489m) region of Gapyung-gun, Kyunggi-do were investigated from April to November, 2001. Total taxa of benthic macroinvertebrates were 105 species, 38 families, 13 orders, 5 classes in 4 phyla. Aquatic insects were 33 species in ephemeroptera, 5 species in odonata, 13 species in plecoptera, 2 species in hemiptera, 1 species in megaloptera, 27 species in trichoptera, 3 species in coleoptera and 13 species in diptera, respectively. Non-insects were 1 species in platyhelminthes, 5 species in gastropoda, 1 species in oligochaeta, 1 species in crustacea, respectively. Ephemeroptera, trichoptera and plecoptera as indicators in clean water were very abundantly. Occurrence species at each survey area was 67 species at Mt. Myungji and 69 species at Mt. Kalbong, 48 species at Mt. Daege.um and 47 species at Mt. Jubal, respectively. Mean species diversity indices at Mt. Myungji, Mt. Kalbong, Mt. Daegeum and Mt. Jubal were 3.30, 3.33, 2.59 and 3.43, respectively. According to the saprobic system based on the species diversity indices of benthic macroinvertebrates, Mt. Myungji, Mt. Kalbong and Mt. Jubal are determined as Limnosaprobic area, respectively, but Mt. Daegeum is determined as $\beta$-mesosaprobic area.

A Comparative Study on the Habitat of Abies koreana Wilson between Mt. Jiri and Mt. Halla

  • lhm, Byung-Sun;Lee, Jeom-sook;Kim, Moon-Hong;Kim, Ha-Song
    • Plant Resources
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    • v.3 no.2
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    • pp.138-147
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    • 2000
  • This examination of vegetation is conducted from June to Oct. 1999 through on the spot survey, centering on the habitat of Abies koreana around Imgeolryeong, Jangteomok, and Jeseokbong in Mt Jiri and Yeongsil path and Seongpanak path in Mt. Halla. Species composition tables of the Abies koreana community by altitude and slope in Mt. Jiri and Mt. Halla are made based on the community composition tables examined in quadrat. The Abies koreana-Saso quelpaertensis community in a west slope of Mt. Halla is found that Abies koreana of 4-6m in height forms subtree layer and that of 8-9m in height the tree layer and it reflects a difference between community structure by slope and main composition species. While the tree layer of Abies koreana community is 12-14 in height around the area of 1,290-1,560m above the sea of Imgeolryong and path from Baekmudong to Jangteomok, it is 8-12m in height in the area of 1,680-1,780m above the sea of path from Jangteomok to Jeseokbong. It means that community structure depends on area and attitude. This study finds out that commonly appearing composition species similar to coverage and presence are Lepisorus thunberianus, Quercus mongolica, Solidago virga-aurea var. asiatica, Ligularia fischeri, Carex lanceolata, Clintonia udensis, Magnolia sieboldii, Betula ermani, Acer pseudo-sieboldianum. As soil environment of surveyed area, pH of surveyed spots is similar in Mt. Jiri and Mt. Halla, electrical conductivity is higher in Mt Jiri, and contents of organic matter is relatively higher in a west slope of Mt. Jiri and Mt. Halla and lower in their south and west slopes. Contents of organic matter and total nitrogen show that the area of 1,550m above the sea in a west slope of Mt. Halla is some higher. For pH and contents of total nitrogen and organic matter, Mt. Halla is higher than Mt. Jiri but for electrical conductivity, Mt. Jiri is relatively higher than Mt. Halla.

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The Structure Analysis of Plant Communities of Betula ermanii Community in Subalpine (아고산대 사스래나무군락의 식물군집구조 분석 연구)

  • Da-Um Yun;Choong-Hyeon Oh
    • Korean Journal of Environment and Ecology
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    • v.38 no.3
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    • pp.338-350
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    • 2024
  • A vegetation survey was conducted and analyzed on 25 survey plots of Betula ermanii communities in the subalpine zone of Mt. Gariwang, Mt. Balwang, Mt. Bangtae, and Mt. Hambaek in Gangwon-do. As a result of TWINSPAN and DCA analysis, the community was divided into a total of 3 communities: Betula ermanii-Quercus mongolica community(GroupI), Betula ermanii-Acer pseudosieboldianum community (GroupII), and Betula ermanii community(GroupIII). As a result of the vegetation structure analysis, Betula ermanii-Quercus mongolica community(GroupI) is located on the periphery of the Mt. Bangtae trail at altitude of 1,082~1,192m, and Betula ermanii-Acer pseudosieboldianum community(GroupII) is located on the ridges and slopes of Mt. Bangtae, Mt. Gariwang, and Mt. Hambaek at altitudes of 1,230~1,373m. Betula ermanii-Quercus mongolica community(GroupI) and Betula ermanii-Acer pseudosieboldianum community(GroupII) are currently dominated by Betula ermanii, but after long term, it is expected that they will transe to Quercus mongolica. The Betula ermanii community(GroupIII) is located the summit of Mt. Hambaek and the summit of Mt. Balwang at altitude of 1,428~1,464m. It is expected that the community will be maintained as Betula ermanii community, because Betula ermanii is very dominant, and so the next generation dominant species is maintained by Betula ermanii. Betula ermanii community generally appear above 1,000 m above sea level, and the higher the altitude, the more dominant the Betula ermanii are. As a result of the soil analysis, the soil characteristics shows a similar pattern to the subalpine belt of Daecheongbong Peak of Mt. Seoraksan and Banyabong Peak of Mt. Jirisan. As a result of the species diversity index analysis, the species diversity index was 1.210~1.293, the abundance index was 0.698~0.795, and the dominance index was 0.208~0.302.

A Study on the Production and Decomposition of Litters Related to Altitude (한라산, 소백산 및 태백산의 고도에 따른 낙엽의 생산과 분해에 관한 연구)

  • Chang, Nam Kee;Hi Chung Kwon
    • The Korean Journal of Ecology
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    • v.10 no.3
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    • pp.109-118
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    • 1987
  • The production and decomposition rates of litters were studied in three mountains, Mt. Halla, Mt. So-back and Mt. Tae-back. The amounts of N, P, K, Ca and Na in the soils were measured and the relationships between the mineral nutrients and decay rates were reserched, The annual litter production was the most as $1, 077.3g/m^2$ and the least as $248.0g/m^2$ in a stand of Quercus acutissima at 820m of the Mt. So-back and at 1, 350m of the Mt. So-back among the study sites, respectively. The decay rates of litter was the highest as k=0.448 and the lowest as k=0.082 in a stand of Q. acutissima at 820m of the Mt. So-back and at 1, 450m of the Mt. Tae-back at 1, 450m of the Mt. Tae-back among the study sites. The higher altitude was, the slower the decay rates were. The annual contents of mineral nutrient and their amounts inputted into the forest soil were proportional to the decay rate of organic metters.

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Mitochondrial DNA polymorphism in Fourteen Geographical Strains of Drosophila melanogoater (세계 14지역 계통에 대한 초파리 미토콘드리아 DNA의 다형현상)

  • 김봉기
    • The Korean Journal of Zoology
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    • v.31 no.3
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    • pp.218-224
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    • 1988
  • Restriction endonucleases were used to search for intraspecific variation at 32 cleavage sites in mitochondrial DNA(mtDNA) purified from fourteen strains of Drosophila melanogaster helonging to different localities of the world. mtDNA of D. melanogaster was displayed site variation(Hpall, Haelll and Seal endonucleases) and length variation(maxirnum 550bp). Six genotypes, Ml, M2, M3, M4, M6 and M7, could be distinguished based on ihe site types witti a low average of intraspecific substitution rate (1.88%),but M5 type of Ogasawara strain in Japan was not detected in this study. A possible explanation for the low divergence was that mtDNA variation of fourteen strains in D. melanogaster could not he accumulated sufficiently owing to recent divergence of few individuals, and that sequence divergence was prevented by frequent migration in spite of the geographical isolation.

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Gene Structure and Altered mRNA Expression of Metallothionein in Response to Metal Exposure and Thermal Stress in Miho Spine Loach Cobitis choii (Cobitidae; Cypriniformes) (미호종개 metallothionein 유전자의 구조 및 중금속 노출과 고온 자극에 대한 MT mRNA의 발현 특징 분석)

  • Lee, Sang-Yoon;Nam, Yoon-Kwon
    • Korean Journal of Ichthyology
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    • v.23 no.1
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    • pp.61-69
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    • 2011
  • Gene and promoter structures of metallothionein(MT) from Miho spine loach (Cobitis choii; Cypriniformes) were characterized, and the transcriptional responses to experimental exposures to heavy metals and heat stress were examined. The C. choii metallothionein displayed well-conserved features of teleostean metallothioneins at gDNA, mRNA and amino acid levels. Bioinformatic analysis predicted that the C. choii MT regulatory region potentially possessed various motifs or elements targeted by various transcription factors associated with metal-coordinating regulation (e.g., metal transcription factor-1), immune responses (e.g., nuclear factor kappa B), and thermal modulations (e.g., heat shock factor). Acute heavy-metal exposures to 0.5 or $1.0\;{\mu}M$ of cadmium (Cd), copper (Cu), manganese (Mn), nickel (Ni) or zinc (Zn) showed that MT transcription was significantly stimulated by Cd (9.6-fold relative to non-exposed control) and Cu (10.4-fold), only moderately by Mn (2.4-fold), but hardly by Ni and Zn. Elevation of water temperature from $25^{\circ}C$ to $31^{\circ}C$ caused a rapid modulation of MT mRNAs toward upregulation to 9.5-fold; however, afterward the elevated mRNA level slightly decreased during further incubation at $31^{\circ}C$ for 6 h. Results from this study suggest that MT-based expression assay could be a useful basis for better understanding the metal- and/or heat-caused stresses in this endangered fish species.

Notes on the Korean Higher Fungi(XVII)

  • Cho, Duck-Hyun
    • Plant Resources
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    • v.5 no.1
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    • pp.51-58
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    • 2002
  • Many higher fungi were collected at Mt.Manrae, Mt.Kirin, Mt.Nam, Mt.Unjang, Mt.Yonsuk, Mt.Minjuji from May, 2000 to June, 2001 and they were identified. As the result, Marasmius buxi, M. epiphylloides, M. hudsoni, Mycena erubescens, Agaricus bisporus var. bisporus, Panellus ringens, Lactarius aquiflus, Leccinum quericinum were newly discovered in Korea. They were designed Korean common names by author.

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Energy Content and Photosynthetic Efficiency of Quercus mongolica Stands in Korea

  • Kwon, Ki-Cheol;Lee, Don Koo
    • Journal of Korean Society of Forest Science
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    • v.95 no.5
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    • pp.562-568
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    • 2006
  • This study was conducted to examine the energy content and photosynthetic efficiency of Quercus mongolica stands in Korea. Study sites were located in Mt. Joongwang, Gangwon-do (1,000 m and 800 m above sea level), Mt. Baekwoon, Jeollanam-do (800 m a.s.l.), Mt. Halla, Jeju-do (1,000 m a.s.l.), Mt. Taehwa, Gyeonggi-do (350 m a.s.l.), and Mt. Wolak, Chungcheongbuk-do (300 m a.s.l.). Total energy content and annual energy accumulation in Q. mongolica stands were 2,916-6,435 GJ/ha and 284-441 GJ/ha, respectively. Lower latitude (N.L.) stands of Q. mongolica showed higher energy contents than higher latitude stands, but Quercus stands in Mt. Baekwoon had higher annual energy accumulation than those in Mt. Halla located at a lower latitude. During the growing season, the photosynthetic efficiency of 60 to 70-year-old Q. mongolica stands ranged from 1.19 to 1.34% while that of 35-year-old stands did from 1.87 to 1.95%. There were no significant differences in photosynthetic efficiency among the latitudes because solar radiation was higher in low latitudes.