• 제목/요약/키워드: MT1

검색결과 2,809건 처리시간 0.037초

형질전환된 Escherichia coli변이주에서 Sodium citrate를 이용한 고농도 L-Threonine 생산 (Hyperproduction of L-Threonine by Adding Sodium Citrate as Carbon Source in Transformed Escherichia coli Mutant.)

  • 이만효;김병진;정월규;최선욱;박해룡;황용일
    • 생명과학회지
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    • 제14권5호
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    • pp.868-873
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    • 2004
  • 유용성이 확보된 L-threonine의 효율적인 발효생산을 위하여 생산균주 E. coli MT201를 유전자재조합을 통하여 개량하고 적절한 탄소원을 발굴하여 전체적인 생산량 증대를 도모하였다. 먼저, 5 liter발효조에서 유가식 배양을 통하여 생산균주 E. coli MT201이 균체량이 52($OD_{660}$)일때 57 g/1의 생산량을 보이는 것을 확인하였다. L-Threonine의 생산성 향상을 위하여 균체 내에서 생합성 전구물질인 oxaloacetate를 충분하게 공급하기 위해 C. glutamicum 유래의 pyruvate carboxylase의 유전자를 plasmid pPYC의 형태로 E. coli MT201에 도입하였다(E. coli MT/PYC). 그렇지만 E. coli MT/PYC을 배양한 결과로부터 E. coli MT201와 비교할 때 균체증식 및 생산량이 모두 감소하는 경향을 보였으며, 이를 해결하기 위하여 플라스크배양을 통하여 포도당과 sodium citrate를 1.5:3.5의 비율로 배지 중에 첨가하였을 때 이들 문제가 개선되는 것을 관찰하였다. 상기 비율의 탄소원 조건하에서 5liter 발효조를 이용한 유가식 배양에서 배양 75시간째에 L-threonine의 생산량 및 균체량($OD_{660}$)이 각각 75.7 g/l와 48로 효율적으로 향상되는 것을 알 수 있었다. 이는 과도한 anaplerosis에 의한 TCA 회로의 불균형을 중간산물인 citric acid를 sodium citrate의 형태로 공급함으로써 E. coli MT/PYC에서 균체증식이 정상화됨을 의미한다.

경상남도 사천시의 식물생태지수 개발을 위한 기초연구 (The Basic Study for Developing Plant Ecological Indices of Sacheon-si, Gyeongsangnam-do)

  • 오현경;유주한
    • 환경영향평가
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    • 제21권4호
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    • pp.509-523
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    • 2012
  • This basic study is to present the methods and raw data for carrying out to objectively assess an ecosystem by developing the plant ecological indices. The results are as follows. The plant ecological indices were 7 units including RI (Rare Index), EI (Endemic Index), SI (Specific Index), NI (Naturalized Index), UI (Urbanized Index), Pte-Q (Pteridophyta Index) and HI (Halophytes Index). The vascular plants in Mt. Waryong, Mt. Bongmyeong and Bito island were summarized as 471 taxa including 104 families, 294 genera, 419 species, 3 subspecies, 41 varieties and 8 forms. We surveyed 5 taxa of rare plants, 7 taxa of endemic plants, 38 taxa of specific plants by floristic region, 40 taxa of naturalized plants, 1 taxa of invasive alien plant, 19 taxa of pteridophyta and 14 taxa of halophytes. Bito island had the highest RI was 1.2%. Mt. Bongmyeong was 1.4% higher than other sites. If the SI of 2.9% was the highest in Bito island. Bito island was the highest NI, and the UI was the hightest in Mt. Waryong. Mt. Bongmyeong was the lowest NI and UI. In Mt. Waryong and Bito island, the highest DI was 9.1%. The Pte-Q of Mt. Waryong was 1.3 higher than the other sites. Bito island, the highest HI was 8.1%. Overall, 1.5% of RI, 1.9% of EI, 2.3% of SI, 8.5% of NI, 14.3% of UI, 9.1% of DI, 1.0 of Pte-Q and 3.0% were analyzed with HI.

벼 5-methyltryptophan 저항성 돌연변이체의 특성 (Characteristics of Rice Mutants Resistant to 5- Methyltryptophan)

  • 이효연;강권규;노일섭;이춘환;권혜경;박현숙
    • 한국작물학회지
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    • 제40권5호
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    • pp.637-643
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    • 1995
  • 5-methyltryptophan (5MT) 저항성 벼 (Oryza sativa L. val. Sasanishiki)로 부터 선발된T-RI의 자식후대개체 중에서 저항성이 우성형질로 분리된 TR75주를 이용하여 그 식물체의 여러 특성을 조사하였다. 그 결과를 요약하면 다음과 같다. 1. TR75는 종자, 근, 약에 있어서는 25 mg/1의 5MT가 포함된 MS배지에서 callus가 형성되었으나, 야생형의 경우에는 어느 기관에서도 Callus 유도는 일어나지 않았다. 2. 각 기관으로 부터 유도된 TR75의 callus는 50mg/1의 5MT배지에서도 저항성을 보여주었지만 야생형의 callus는 25mg/1의 5MT 배지에서 모두 갈변화 하였다. 3. TR75는 3종류의 아미노산 아날로그(L-azetidine-2-carboxylic acid, S-2-aminoethyl-L-cystene, p-fluoro-DL-phenylalanine)에 대해서는 저항성을 갖고 있지 않다. 4. TR75의 유리아미노산 함량은 야생형 종자에 비해 tryptophan, phenylalanine, aspartic acid가 각각 5.0, 5.3, 2.7 배 증가하였으며, 그 외 다른 종류의 아미노산 함량도 1.5∼2.0배 증가하였다. 전 아미노산 함량도 약 1.5배 가량 증가하였다. 그러나 alanine과 leucine은 약간 감소하였다.

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황폐임야(荒廢林野)가 수해참상(水害慘狀)에 미치는 영향(影響) (천보산(天寶山)과 인접(隣接) 죽엽산(竹葉山)을 중심(中心)으로) (Influences of the devastated forest lands on flood damages (Observed at Chonbo and the neighbouring Mt. Jook-yop area))

  • 정인구
    • 한국산림과학회지
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    • 제5권1호
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    • pp.4-9
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    • 1966
  • 1. On 13 September 1964 a storm raged for 3 hours and 20 minutes with pounding heavy rainfalls, and precipitation of 287.5 mm was recorded on that day. The numerous landslides were occured in the eroded forest land neighbouring Mt. Chunbo, while no landslides recorde at all on Mt. Jookyup within the premise of Kwangnung Experiment Station, the Forest Experiment Station. 2. Small-scalled Landslides were occured in 43 different places of watershed area (21.97 ha.) in which the survey had already been done, in and around Mt. Chunbo (378 m a.s.l.). The accumulated soil amount totaled $2,146,56m^3$ due to the above mentioned landslides, while soil accumulated from riverside erosion has reached to $24,168.79m^3$, consisting of soils, stones, and pebbles. However, no landslides were reported in the Mt. Jook yup area because of dense forest covers. The ratio of the eroded soil amount accumulated from the riversides to that of watershed area was 1 to 25. On the other hand, the loss and damage in the research area of Mt. Chonbo are as follows: 28 houses completly destroyed or missing 7 houses partially destroyed 51 men were dead 5 missing, and 57 wounded. It was a terrible human disaster However, no human casualties were recorded at all, 1 house-completly destroyed and missing, 2 houses-partially destroyed. Total:3 houses were destroyed or damaged, in The area of Mt. Jookyup 3. In the calculation of the quanty of accumulated soil, the or mula of "V=1/3h ($a+{\sqrt{ab}}+b$)" was used and it showed that 24, 168.79m of soil, sands, stones and pebbles carried away. 4. Average slope of the stream stood 15 at the time of accident and well found that there was a correlation between the 87% of cross-area sufferd valley erosion and the length of eroded valley, after a study on regression and correlation of the length and cross-area. In other works, the soil erosion was and severe as we approached to the down-stream, counting at a place of average ($15^{\circ}1^{\prime}$) and below. We might draw a correlation such as "Y=ax-b" in terms of the length and cross-area of the eroded valley. 5. Sites of char-coal pits were found in the upper part of the desert-like Mt. Chunbo and a professional opinion shows that the mountain was once covered by the oak three species. Furthermore, we found that the soil of both mountains have been kept the same soil system according to a research of the soil cross-area. In other words, we can draw out the fact that, originally, the forest type and soil type of both Mt. Chunbo (378m) and Mt. Jookyup (610m) have been and are the same. However, Mt. Chunbo has been much more devastated than Mt. Jookyup, and carried away its soil nutrition to the extent that the ratios of N. $P_2O_5K_2O$ and Humus C.E.C between these two mountains are 1:10;1:5 respectively. 6. Mt. Chunbo has been mostly eroded for the past 30 years, and it consists of gravels of 2mm or larger size in the upper part of the mountain, while in the lower foot part, the sandy loam was formulated due to the fact that the gluey soil has been carried and accumulated. On the hand, Mt. Jookyup has consitantly kept the all the same forest type and sandy loam of brown colour both in the upper and lower parts. 7. As for the capability of absorbing and saturating maximum humidity by the surface soil, the ratios of wet soil to dry soil are 42.8% in the hill side and lower part of the eroded Mt. Chunbo and 28.5% in the upper part. On the contrary, Mt. Jookyup on which the forest type has not been changed, shows that the ratio in 77.4% in the hill-side and 68.2% in the upper part, approximately twice as much humidity as Mt. Chunbo. This proves the fact that the forest lands with dense forest covers are much more capable of maintaining water by wood, vegitation, and an organic material. The strength of dreventing from carring away surface soil is great due to the vigorous network of the root systems. 8. As mentioned above, the devastated forest land cause not only much greater devastation, but also human loss and property damage. We must bear in mind that the eroded forest land has taken the valuable soil, which is the very existance of origin of both human being and all creatures. As for the prescription for preventing erosion of forest land, the trees for furtilization has to be planted in the hill,side with at least reasonable amount of aertilizer, in order to restore the strength of earth soil, while in the lower part, thorough erosion control and reforestation, and establishments along the riversides have to be made, so as to restore the forest type.

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PCR-RFLP를 이용한 파방나방 (Spodoptera exigua(H bner)) 미토콘트리아 DNA의 유전변이 연구 (Study on the Genetic Variation of the Mitochondrial DNA in the Beet Armyworm, Spodoptera exigua (H bner), Using PCR-RFLP)

  • 김용균;이명렬;정충렬
    • 한국응용곤충학회지
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    • 제37권1호
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    • pp.23-30
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    • 1998
  • DNA의 제한요소단편 다형현상(RFLP)이 유전변이 연구에 널리 이용되고 있다. 본 연구는 파밤나방(Spodoptera exigua(H bner)) 미토콘드리아 DNA(mtDNA)의 RFLP방법을 개발하기 위해 게놈 크기 측정 및 PCR primer들을 선발하였다. 파밤나방의 mtDNA 전체크기는 약 16kb였다. 대부분 곤충 mtDNA에 적합하게 구성된 (Simon et al., 1994)29개 promer들중 21개가 파밤나방의 mtDNA증폭에 적합했다. 이들 primer들을 이용하여 여러 유전자 영역(CO-I, CO-II, Cyt-B, ND-1, 12S rRNA, 16S rRNA 및 일부 tRNA)의 일분 또는 전체를 포함하는 유전자 절편을 증폭시켰다. 일반적으로 다형을 보이는 primer조합을 중심으로 4염기 제한부위를 인식하는 8종의 제한 효소를 통해 분석된 PCR-RFLP는 서로 다은 지역(안동, 경산, 순천) 집단들간에 제한부위에 있어서 차이가 없었으나 일부 영역에서는 길이 차이를 보여 유용한 유전지표로서의 가능성을 제시했다.

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BrMT3 고발현에 의한 애기장대의 카드뮴 저항성 증진 (Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis)

  • 김선하;송원용;안영옥;이행순;곽상수;최관삼
    • Journal of Plant Biotechnology
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    • 제36권1호
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    • pp.68-74
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    • 2009
  • B. rapa로부터 분리한 BrMT3 유전자를 도입시킨 효모와 애기장대가 카드뮴을 비롯한 중금속에 저항성을 보이는 것이 확인되었고 이 결과를 토대로 이 유전자가 중금속 흡착을 통한 환경 정화 및 스트레스에 내성을 갖는 형질전환 식물체를 개발하는데 유용하게 사용될 것으로 기대된다.

덕유산과 계방산 가문비나무 군락의 식생구조에 관한 연구 (Vegetation Structure of Picea jezoensis Communities in Mt. Deogyu and Mt. Gyebang)

  • 조민기;정재민;정혜란;강미영;문현식
    • 농업생명과학연구
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    • 제46권6호
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    • pp.33-41
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    • 2012
  • 본 연구는 덕유산, 계방산의 가문비나무 군락의 보존을 위한 기초자료를 제시하기 위하여 종조성과 흉고직경급을 조사, 분석하였다. 두 조사지의 교목 아교목 관목층의 층위별 식생은 덕유산이 8, 20, 26종, 계방산이 12, 23, 33종으로 구성되어 있다. 층위별 중요치 분석 결과, 덕유산 교목층은 가문비나무, 사스래나무, 구상나무, 아교목층은 사스래나무, 신갈나무, 관목층은 조릿대가 중요치가 높았으며, 계방산의 교목층은 가문비나무, 사스래나무, 분비나무, 아교목층은 시닥나무, 부게꽃나무, 관목층은 미역줄나무의 중요치가 가장 높은 것으로 분석되었다. 조사구의 층위별 종다양도의 경우 교목층은 덕유산 0.779, 계방산 0.984, 아교목층은 덕유산 1.052, 게방산 1.161, 관목층은 덕유산 0.823, 계방산 1.304로 분석되었다. 흉고직경 분포에서는 덕유산지역의 가문비나무 개체군이 역J형의 유형이 나타나고 있어 당분간 가문비나무군락이 유지될 수 있을 것으로 판단된다.

Infection with Citrobacter rodentium in μMT Knockout Mice

  • Jo, Minjeong;Hwang, Soonjae;Rhee, Ki-Jong
    • 대한의생명과학회지
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    • 제24권1호
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    • pp.1-8
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    • 2018
  • ${\mu}MT$ knockout mice are genetically deficient in the transmembrane domain of mu chain of the immunoglobulin M (IgM) heavy chain, resulting in the absence of mature B cells. ${\mu}MT$ knockout mice is an in vivo model system used to clarify the role of B cells in various diseases. Enteropathogenic Escherichia coli (EPEC) induces acute and chronic diarrheal disease, especially in children of developing countries. The formation of attaching and effacing (A/E) lesion is a prominent pathogenic factor in the intestinal epithelium of EPEC infection. The A/E lesion is modulated by genes located on the pathogenic island locus of enterocyte effacement (LEE) which encode a type III secretion system (T3SS) and A/E lesion-related effector proteins. Citrobacter rodentium is a murine pathogen utilized in studying the pathogenic mechanisms of EPEC in human infections. Citrobacter rodentium produce A/E lesion to attach to intestinal epithelium, thus providing a murine model pathogen to study EPEC. Several studies have investigated the pathogenesis of Citrobacter rodentium in the ${\mu}MT$ knockout mice. In this review, we introduce the ${\mu}MT$ murine model in the context of C. rodentium pathogenesis and describe in detail the role of B cells and antibodies in this disease.

Gene Therapy for Mice Sarcoma with Oncolytic Herpes Simplex Virus-1 Lacking the Apoptosis-inhibiting Gene, icp34.5

  • Lan, Ping;Dong, Changyuan;Qi, Yipeng;Xiao, Gengfu;Xue, Feng
    • BMB Reports
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    • 제36권4호
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    • pp.379-386
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    • 2003
  • A mutant herpes simplex virus 1, mtHSV, was constructed by inserting the E. coli beta-galactosidase gene into the loci of icp34.5, the apoptosis-inhibiting gene of HSV. The mtHSV replicated in and lysed U251 (human glioma cells), EJ (human bladder cells), and S-180 (mice sarcoma cells), but not Wish (human amnion cells) cells. With its intact tk (thymidine kinase) gene, mtHSV exhibited susceptibility to acyclovir (ACV), which provided an approach to control viral replication. An in vivo test with mtHSV was conducted in immune-competent mice bearing sarcoma S-180 tumors, which were treated with a single intratumoral injection of mtHSV or PBS. Tumor dimensions then were measured at serial time points, and the tumor volumes were calculated. Sarcoma growth was significantly inhibited with prolonged time and reduced tumor volume. There was microscopic evidence of necrosis of tumors in treated mice, whereas no damage was found in other organs. Immunohistochemical staining revealed that virus replication was exclusively confined to the treated tumor cells. HSV-1 DNA was detected in tumors, but not in the other organs by a polymerase chain reaction analysis. From these experiments, we concluded that mtHSV should be a safe and promising oncolytic agent for cancer treatment.

Carbon Storage of Quercus mongolica Stands by Latitude and Altitude in Korea

  • Kwon, Ki-Cheol;Lee, Don Koo
    • 한국산림과학회지
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    • 제95권2호
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    • pp.220-231
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    • 2006
  • The study was conducted to investigate the differences in carbon storage of Quercus mongolica stands with respect to latitude and altitude in Korea. Study sites were located in Mt. Joongwang, Pyeongchang-gun, Gangwon-do (altitudes 1,300 m, 1,000 m, and 800 m), Mt. Taehwa, Gwangju-si, Gyeonggi-do (altitude 350 m), Mt. Wolak, lecheon-si, Chungcheongbuk-do (altitude 300 m), Mt. Baekwoon, Gwangyang-si, Jeollanam-do (altitude 800 m), and Mt. Halla, Jeju-do (altitude 1,000 m). Total carbon storage and annual carbon storage of Q. mongolica stands were 85-210 tonC/ha and 7.2-10.6 tonC/ha, respectively. Lower latitude (NE) stands of Q. mongolica showed more carbon storage and annual carbon storage than higher latitude stands. Carbon storage and annual carbon storage of Q. mongolica stands were increased in low altitude. Carbon storage of Q. mongolica stands was higher in the northern aspect than in the southern aspect. However, there were no significant differences in annual carbon storage between the aspects.