• Reproductive and Developmental Biology
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• 제34권3호
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• pp.143-151
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• 2010

Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.

• 한국식품영양학회지
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• 제24권4호
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• pp.803-807
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• 2011

Human umbilical Mesenchymal Stem Cell(uMSC) has been known as one of major component to regenerate connective tissues such as bone, cartilage, fat and others. The effect of low(5%), normotensive(20%) oxygen and freezing-thawing damage on proliferation of uMSC were investigated. low oxygen concentration culture of uMSC resulted in enhanced proliferation significantly($p$ <0.05) than 20% of oxygen culture. After the freezing-thawing injury to uMSC, 5% oxygen culture showed marked proliferation of uMSC than that of 20% oxygen($p$ <0.05) in the 5th passage of uMSC. Expression of antioxidant enzymes such as superoxide anion 1 and glutathione peroxidase 1 appeared marked in 20% oxygen cultured uMSC, which suggest oxidative stress could induce less proliferation of uMSC. Above findings would suggest proliferation of uMSC in 5% of oxygen will give more yields.

• 대한산업공학회지
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• 제30권4호
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• pp.277-284
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• 2004

CDMA (Code Division Multiple Access) is a promising air interface technique for digital cellular systems. The soft handoff between base stations is one of many important features of CDMA for the mobile stations crossing the cell boundaries. The service areas of MSC's (Mobile Switching Centers) are defined as the unions of the service areas of the base stations connected to MSC's and are assumed to have hexagonal shapes. An analytical approach to the performance analysis of the link between MSC's for supporting the inter-MSC soft handoff scheme will be developed to obtain the probability that a soft handoff to an adjacent MSC will be blocked due to the shortage of the link capacity. Also, the rate of new connection establishments that are requested by the mobile stations moving to the service area of an MSC according to the inter-MSC soft handoff scheme will be obtained.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.530-532
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• 2007

Multi-Spectral Camera(MSC) is a main payload on the KOMPSAT-2 satellite to perform the earth remote sensing. The MSC instrument has one(1) channel for panchromatic imaging and four(4) channel for multi-spectral imaging covering the spectral range from 450nm to 900nm using TDI CCD Focal Plane Array (FPA). The compression method on KOMPSAT-2 MSC was selected and used to match EOS input rate and PDTS output data rate on MSC image data chain. At once the MSC performance was carefully handled to minimize any degradation so that it was analyzed and restored in KGS(KOMPSAT Ground Station) during LEOP and Cal./Val.(Calibration and Validation) phase. In this paper, on-orbit image data chain in MSC and image data processing on KGS including general MSC description is briefly described. The influences on image performance between on-board compression algorithms and between performance restoration methods in ground station are analyzed and discussed.

• 대한산업공학회지
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• 제26권4호
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• pp.336-344
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• 2000

The soft handoffs between two adjacent MSC's should be employed to support the calls requesting handoffs to an MSC while minimizing the undesirable ping pong phenomenon of back-and-forth handoffs between two adjacent cells in conventional hard handoffs. In this paper, the soft handoff scheme between two MSC's is considered using the trunk between the packet routers for the two MSC's. The trunk network is proposed to support the inter-MSC soft handoff scheme in the service area with many MSC's. The probability that a soft handoff to an adjacent MSC will be blocked due to the shortage of the trunk capacity is derived.

• Childhood Kidney Diseases
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• 제14권1호
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• pp.32-41
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• 2010

목 적 : 사구체신염은 흔히 단백뇨를 보이며 특이 치료법이 없고, 만성 신부전으로 발전하는 경우가 많다. 몇몇 연구에서 중간엽 줄기세포(Mesenchymal stem cell, MSC)를 실험적 사구체신염에 투여하여 단백뇨가 호전된 것을 보고한 바 있으나, 이는 신염을 일으키는 약제와 중간엽 줄기세포를 함께 투여하거나 신장에 직접 투여한 것이었다. 본 연구에서는 실험적 신병증에서 단백뇨가 발현된 시점에서 정주 요법으로 MSC를 투여함으로써 MSC의 임상적인 적용 가능성을 탐색하였다. 방 법 : 실험용 생쥐에 Adriamycin을 투여하여 신병증(ADR-GN)을 유발한 후, 2주 후에 대량의 단백뇨를 확인하고 MSC를 생쥐 꼬리의 정맥에 주사하였다. MSC에 의한 질병 완화의 기전을 확인하기 위한 in vitro 실험으로 mixed lymphocyte culture(MLC)에 MSC를 투여하였을 때의 염증 관련 cytokine인 IFN-$\gamma$ and IL-10의 변화를 측정하였다. 결 과 : 실험용 생쥐에 ADR-GN를 유발하고 단백뇨가 보일 때 MSC를 정주한 군에서는 단백뇨의 소실이 더 먼저 관찰되었다. 또한 MSC를 투여받은 군에서의 생존률이 더 나은 경향이 관찰되었다. MLC 에 MSC를 투여하였을 때, 염증을 유발하는 cytokine인 IFN-$\gamma$ 는 감소하고 염증을 억제하는 cytokine인 IL-10는 증가하였다. 결 론 : 이 연구는 이전의 보고들에서 관찰되었던 사구체신염에서의 MSC의 질병완화 효과가 좀더 임상적으로 적용 가능한 방법으로 투여된 경우, 즉 단백뇨가 있을 때 정주 요법으로 투여한 경우에도 관찰됨을 확인하였다. 이러한 효과의 기전과 임상적용에 요구되는 안전성 등에 대한 확인을 위해서는 추가 연구가 필요하겠다.

• 대한원격탐사학회지
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• 제17권3호
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• pp.211-218
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• 2001

대기복사모델인 MODTRAN을 이용해 다목적실용위성 2호 탑재체인 Multispectral Camera (MSC)의 총복사량에 대한 계산을 수행하고 그 결과를 분석해 보았다. 모델 계산에서 4계절 조건을 모의실험하기 위해 1월 15일, 7월 15일 계산에 대해 중위도 동절기 및 하절기 모델대기를, 4월 15일, 10월 15일에 대해 US 표준대기를 각각 사용했다. 다목적실용위성 2호 궤도 조건과 각 계절에 대한 대표적인 태양천정각 (solar zenith angle)이 고려되었다. 시정거리는 대류권 에어로솔 소광계수 (tropospheric aerosol extinction)에 해당하는 50km가, 지표의 알베도는 맑은 날 지구 연평균 값에 해당하는 0.135가 가정되었다. MSC 계약서 값은 위 일반적 조건을 가정하고 얻은 모델 계산 총복사량보다 MSC 관측 파장대역 대부분에서 상당히 크다는 것을 알게 되었다. 또한 균일한 지표 알베도를 가정하고 얻은 모델 결과의 분광파장 특징이 MSC 계약서 값의 경향과 다름을 보였다. 이들 결과로부터 향후 획득될 MSC영상은 비교적 어두운 영상이 될 것으로 추론된다.

• 한국GIS학회:학술대회논문집
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• 한국GIS학회 2004년도 GIS/RS 공동 춘계학술대회 논문집
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• pp.255-259
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• 2004

본 논문에서는 다목적 위성 2호의 주 탑재체인 MSC (Multi-Spectral Camera)의 영상자료 검보정을 위한 검보정 target 준비 작업에 대해 설명한다. MSC 영상 자료에 대한 검보정 작업은 다목적 위성 2호의 발사 후 초기 운영 기간 (LEOP: Launch and Early Operation Phase)인 3개월 동안 수행될 예정이다. 위성 발사 전까지 MSC 영상 자료에 대한 검보정을 수행하기 위해 필요한 준비 작업들이 현재 한국항공우주연구원에서 진행중이다. LEOP 기간 동안 MSC 영상 자료를 검보정하기 위해서, MSC의 센서 특성에 따라 7가지 정도의 검보정 target에 대한 설계 초안이 완성되었으며, 향후 target에 대한 설계를 완성한 후에 2004년 중에 한 두 부지에 몇 가지 target들을 건설하고, 다목적 위성 2호의 궤도 특성을 고려하여 일부 target은 운반이 가능하도록 제작할 예정이다. 검보정 target이 촬영된 MSC 영상 자료의 분석을 통해, GSD (Ground Sample Distance), Aliasing, Linearity, Edge Slope & Response, MTF (Modulation Transfer Function), FOV & IFOV, Absolute radiometric validation, Position Accuracy 등의 MSC 검보정 요소 값들을 측정할 계획이다.

• 한국정보통신설비학회:학술대회논문집
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• 한국정보통신설비학회 2008년도 정보통신설비 학술대회
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• pp.495-499
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• 2008

Last year UMTS (UMTS: Universal Mobile Telecommunication System) 3G service started and these days 3 its service subscribers sharply increased. Now totally we have been increasing 13 million subscribers, every month 0.8 million people join 3G Service. MSC (Mobile Switching Center) is most important equipment in 3G system, so we call it 'core' network. Higher capacity MSC required to accommodating 1 million subscribers. It is very important whether MSC can accommodate maximum subscriber or not. So systematic analysis, reliable test results are required. This article presents WCDMA MSC performance evaluation case. This would be some direction for designing and developing some communication equipment. This Case Study demonstrates our MSC system performance.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제39권
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• pp.8.1-8.8
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• 2017

Background: For an effective bone graft for reconstruction of the maxillofacial region, an adequate vascular network will be required to supply blood, osteoprogenitor cells, and growth factors. We previously reported that the secretomes of bone marrow-derived mesenchymal stem cells (MSC-CM) contain numerous growth factors such as insulin-like growth factor (IGF)-1, transforming growth factor $(TGF)-{\beta}1$, and vascular endothelial growth factor (VEGF), which can affect the cellular characteristics and behavior of regenerating bone cells. We hypothesized that angiogenesis is an important step for bone regeneration, and VEGF is one of the crucial factors in MSC-CM that would enhance its osteogenic potential. In the present study, we focused on VEGF in MSC-CM and evaluated the angiogenic and osteogenic potentials of MSC-CM for bone regeneration. Methods: Cytokines in MSC-CM were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVECs) were cultured with MSC-CM or MSC-CM with anti-VEGF antibody (MSC-CM + anti-VEGF) for neutralization, and tube formation was evaluated. For the evaluation of bone and blood vessel formation with micro-computed tomography (micro-CT) and for the histological and immunohistochemical analyses, a rat calvarial bone defect model was used. Results: The concentrations of IGF-1, VEGF, and $TGF-{\beta}1$ in MSC-CM were $1515.6{\pm}211.8pg/mL$, $465.8{\pm}108.8pg/mL$, and $339.8{\pm}14.4pg/mL$, respectively. Tube formation of HUVECs, bone formation, and blood vessel formation were increased in the MSC-CM group but decreased in the MSC-CM + anti-VEGF group. Histological findings suggested that new bone formation in the entire defect was observed in the MSC-CM group although it was decreased in the MSC-CM + anti-VEGF group. Immunohistochemistry indicated that angiogenesis and migration of endogenous stem cells were much more abundant in the MSC-CM group than in the MSC-CM + anti-VEGF group. Conclusions: VEGF is considered a crucial factor in MSC-CM, and MSC-CM is proposed to be an adequate therapeutic agent for bone regeneration with angiogenesis.