• Title/Summary/Keyword: MRS

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Nitrite depletion and Antimicrobial activity of lactic acid bacteria isolated from Kimchi. (김치에서 분리된 유산균의 Nitrite 소거능과 항균성)

  • 이신호;박나영
    • Microbiology and Biotechnology Letters
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    • v.28 no.1
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    • pp.39-44
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    • 2000
  • This study was carried out to develop a new starter culture for the fermented meat products. Nine strains of lactic acid bacteria isolated from kimchi inhibited the growth of Listeria monocytogenes. Among these nine strains, three strains showing antimicrobial activities against Escherichia coil, Staphylococcus aureus and Vibrio paraphaemolyticus were selected for further study. Growth of the strains was inhibited in MRS broth containing 5% of NaCl at $21^{\circ}C$, but not at $32^{\circ}C$. Nitrite depletion ratio of the strains was above 70% after 48h incubation at $21^{\circ}C$, and above 90% after 48h at $32^{\circ}C$ in MRS broth containing $200\mu$g/ml of nitrite, Nitrite concentration of cured meats and ground meats was depleted from 87.6% to 92.3% and from 45.5 to 640.6% by addition of the selected strains for 24h at $32^{\circ}C$, respectively. Three strains were identified as Lactobacillus plantarum(N4) and Lactobacillus lactis ssp. lactis(N-7, an-8).

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A Study on the Historical Characters and Textile Conservation of Mrs. Lee, Dan-ha's Wonsam (외제 이단하 부인 대예복(원삼)에 대한 연구)

  • Bai, Sang Kyoung
    • Journal of Conservation Science
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    • v.5 no.2 s.6
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    • pp.15-24
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    • 1996
  • Th)s study was carried out of the historical characters and textile coservations for Mrs. Danha Lee's Wonsam. As the textile material was silk, dry cleaning method was suggested. The solvents for dry cleaning were n-hexane, n-decane, and benzene. During the second cleaning process, the dry soap(HI-TECH, 120:1, volume ratio) was added to the mixed solvents. The reaction's temparature was $20^{\circ}C$, and the reaction's time was 30 minuutes. It seemed to be a gift for hot from the Royal Family because of the attachment of pheonlx hungbae. This wonsam was decorated symmetrically by gold weaving yarn, the basic fabric was green silk satin with glorius letters and floral patterns. It was made on the 17th C and the oldest thing among them.

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Design Evolution and Spatial Composition of Schindler's Demolished Cabin for Mr. and Mrs. Popenoe of 1922 at Coachella, California

  • Park, Jin-Ho;Lee, Hong-Kyu;Joo, Yong-Sun;Cho, Young-Ho
    • Architectural research
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    • v.9 no.2
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    • pp.11-18
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    • 2007
  • A cabin for Mr. and Mrs. Popenoe of 1922 was designed by the eminent Los Angeles architect, Rudolph M. Schindler. It stands out as an early exemplar of Schindler's most notable work in its unique employment of compositional strategy. Unfortunately, the cabin was demolished before an in-depth research was executed. In addition, there remains no documentary record with regard to the construction process, structural details and the use of materials of the built cabin. However, a set of drawings of the house are housed in the Schindler Archive. Reworking drawings and fabricating a scale model based on the materials obtained from the Archive, this article first depicts the evolution of the design, and then, attempts to investigate underlying principles governing the spatial composition of the cabin.

Correlation between Metabolite Peak Area Ratios on the Influence of Poor Shimming by $^1H$ MR Spectroscopy

  • Baik, Hyun-Man;Choe, Bo-Young;Suh, Tae-Suk;Lee, Hyuong-Koo
    • Journal of the Korean Magnetic Resonance Society
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    • v.3 no.2
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    • pp.140-148
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    • 1999
  • Using 1H magnetic resonance spectroscopy (MRS), we quantitatively evaluated correlation representing linear relationship between the metabolite peak area ratios associated with poor shimming conditions. The inadequate shimming due to linear shim offsets directly affected overall MR spectral quality as well as peak area for each metabolite. Three major peaks such as N-acetylaspartate (NAA), creatine (Cr,) choline (Cho) were used as a reference for data analysis. Despite considerable variations of metabolite peak area, a significant correlation between the metabolite peak area ratios relative to Cr was established while the correlation between the peak area ratios relative to Cho and NAA was not. The present study suggested that metabolite peak area ratios based on the metabolite of Cr could be an acceptable quantification method even under the poor shimming in clinical MRS examination.

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도축 폐혈액 단백질을 이용한 유산균체의 생산

  • 현창기;신현길
    • Microbiology and Biotechnology Letters
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    • v.25 no.2
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    • pp.218-223
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    • 1997
  • For the utilization of animal blood produced in slaughter for the cultivation of lactic acid bacteria, the nitrogen sources in a complex(MRS) medium were replaced by blood plasma proteins. Focusing the purpose on the industrial production of a probiotics, the hydrolytic activities of three industrially applicable proteases were compared for the effective digestion of the proteins, and Alcalase(the product of Novo Nordisk) was selected with comparatively high activity. The growth of Streptococcus thermophilus KCCM12020 was best among the four strains of lactic acid bacteria tested. With Alcalase-digested proteins in the medium, the growth rates and the final cell concentrations were higher than those with non-digested proteins. The cell mass produced in the medium containing blood proteins as nitrogen sources, $2.5{\times}10^9$ CFU/ml, was significantly high and about 70% of that in MRS medium, showing a great possibility for the utilization of animal blood proteins as economic nitrogen sources in the production of cell mass of lactic acid bacteria.

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김치에서 분리한 유산균의 생육에 미치는 오미자 (Schizandra chinensis) 추출물의 영향

  • 이신호;임용숙
    • Microbiology and Biotechnology Letters
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    • v.25 no.2
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    • pp.224-228
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    • 1997
  • This studies were carried out to investgate the effects of omija (Schizandra chinensis) extracts(OE) on the growth of lactic acid bacteria (LAB, HYO-1 and HYO-2) isolated from home-made kimchi. The growth of isolated LAB was inhibited apparently in modified MRS broth containing 1% or 2% of OE. The growth of isolated LAB was inhibited by pH control with HCI to 4.3 or 3.7, the same pH of modified MRS broth containing 1% or 2% of OE. The strong growth inhibitory properties against isolated LAB were shown by fumaric and itaconic acid among various organic acids of Schizandra chinensis, such as citric acid, malic acid, lactic acid, fumaric acid, itaconic acid and acetic acid. The isolated LAB were indentified as Lactobacillus plantarum.

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An interconnection, modelling and simulation for a multi-robot systems(MRS) (다중 로봇 시스템의 결합, 모델링 및 시뮬레이션)

  • 이기동;홍지민;이범희;고명삼
    • 제어로봇시스템학회:학술대회논문집
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    • 1991.10a
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    • pp.1149-1154
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    • 1991
  • For a robotic workcell, which consists of multiple robots, several interconnection methods are presented in terms of the processor based architecture. Since few attempts have been made to formulate and analyze multiple robot system(MRS), we turn the knowledge of multiple processor system(MPS) or multiple computer system(MCS) to good account. The performance evaluation is achieved through queueing analysis, the aim being to compare their response time, utilization, probability of service failure under different workload. To verify the validity of the proposed analysis methods, a computer simulation is performed. The results together with comments presented here give some useful guidelines for the selection of an appropriate interconnection method.

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The Effect of Jujube Extract on the Growth of Lactic Acid Bacteria (대추 추출물이 유산균의 생육에 미치는 영향)

  • Jung Seung-Won;Noh Wan-Seob
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.3
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    • pp.349-356
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    • 2006
  • This study was carried out to survey the effect of Jujube extract on the growth of 3 strains of lactic acid bacteria(LBA) starter cultures in the MRS broth by the addition of 0, 0.1, 0.5, 1 and 2% extract The pH, titratable acidity and OD of LAB were investigated in order to get fundamental knowledge for the development a new product. The effects of Jujube extract on the growth of LAB were variable depending upon the LAB species and concentrations of Jujube extract significantly (p<0.05). In the results, Jujube extract enhanced the acid production and propagation by the 3 LAB strains with increasing concentration of Jujube extracts until 2.0% added was to the MRS broth medium (p<0.05). Addition of Jujube extract markedly stimulated the acid production and propagation of Lactobacillus acidophilus, Streptococcus thermophilus and Bifidobacterium longum.

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The Excavated Costume front Mrs. Jung(1665~1614)'s Grave - Focused on the Funeral Custom and Shroud in the Early 17th Century - (장기 정씨(1565~1614) 묘의 출토복식 - 17세기 초 장속과 습의를 중심으로 -)

  • 이은주
    • Journal of the Korean Society of Costume
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    • v.53 no.3
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    • pp.73-87
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    • 2003
  • The custom and shroud for funeral rites of the early 17th Century based on the excavated costume from the grave of Mrs. Jung (1565 - 1614) are studied in this paper. The grave was located in the Pohang, Kyungbuk Province and was excavated in, April 1999. The remains consist of one set of epitaphs, 33 pieces of costume, 7 pieces of cloths, and 14 pieces of funeral accessaries. From these remains, this study newly reveals : 1) a specific funeral custom for the upper class from the fact that her husband, Ja-Hun Ki, was in the position of Prime Minister for the Chosun government when she died, 2) a fact that women also wore a formal dress with round-shaped collar (Dalryoung) and a concubine could use rectangular tablets ((Hyungbae) with peacock pattern according to her husband's rank, 3) a whole set of shroud used for women in the early 17th Century, and 4) specific construction methods for individual shroud.

Isolation and Identification of Acid- and Bile-Tolerant Lactobacillus salivarius subsp. salivarius from Human Faeces

  • Bae, Hyoung Churl;Choi, Seong Hyun;Nam, Myoung Soo
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.8
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    • pp.1170-1178
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    • 2001
  • This study was conducted to isolate lactobacilli having characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from human faeces. Bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. Microhemagglutination tests using swine erythrocytes were performed to select lactobacilli having adherence properties to survive in the intestinal tract. By examination of these characteristics the strain Nam 27, which was isolated from adult faeces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing.