• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.2
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• pp.268-274
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• 2015

Makgeolli residue protein (MRP) was extracted from byproduct of makgeolli processing, and MRP films containing various plasticizers were prepared. Among the plasticizers used in this study, MRP film containing glycerol-sorbitol (1:2) showed the most desirable mechanical properties. In addition, MRP films containing wasabi extract (WE) were prepared by incorporating different amounts (0, 0.8, 1.0, and 1.2%) of WE into film-forming solution. Tensile strength, elongation at break, and moisture content of MRP films decreased with addition of WE as compared with the control. However, MRP films containing WE showed antimicrobial activities against Escherichia coli O157:H7 and Listeria monocytogenes. Application of MRP film containing 1.0% WE to beef packaging decreased populations of E. coli O157:H7 and L. monocytogenes after storage at $4^{\circ}C$ for 8 days by 1.1 and 0.41 log CFU/g, respectively, compared with those of the control. In addition, the peroxide value and 2-thiobarbituric acid reactive substance value decreased by 53 and 56%, respectively, compared to the control. Therefore, these results suggest that MRP film containing WE can be used to improve the quality of beef during storage.

• Korean Management Science Review
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• v.5 no.2
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• pp.16-24
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• 1988

본 연구에서는 MRP시스템을 도입하여 현재 사용하고 있는 우리나라 제조업체들을 대상으로 MRP시스템의 도입 및 적용과정과 관련된 여러 상황을 조사하였다. 다시 말하면 MRP사용업체들의 현주소를 파악하는 것이 본 연구의 주된 목적이다. 시간의 제약 때문에 MRP사용업체들의 파악이 순조롭게 진행되지 않아서 대상기업들의 수효가 많지 않고, 시스템의 도입 역사가 일천한 관계로 본 연구는 MRP시스템의 적용과정에 대한 예비조사로 규정한다. 그리고 본 연구의 경험을 기반으로 차후에 보다 광범위하고 세밀한 연구가 이루어질 것으로 기대한다.

• Annals of Surgical Treatment and Research
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• v.95 no.5
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• pp.249-257
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• 2018

Purpose: Multidrug resistance-associated protein (MRP) 2 is a glutathione conjugate in the canalicular membrane of hepatocytes. Early graft damage after liver transplantation (LT) can result in alteration of MRP2 expression. The purpose of this study was to evaluate the relationship between the pattern of MRP2 alteration and graft outcome. Methods: Forty-one paraffin-embedded liver graft tissues obtained by protocol biopsy within 2 months after LT; these were stained using monoclonal antibodies of MRP2. We selected 15 live donor biopsy samples as a control, that showed homogenous canalicular staining for MRP2. The pattern of canalicular MRP2 staining of graft was classified into 3 types: homogenous (type C0), focal (type C1), and no (type C2,) staining of the canaliculi. Results: In total, 17.1% graft tissues were type C0, 36.6% were type C1, and 46.3% were type C2. The median operation time was longer in patients with type C2 (562.6 minutes) than in patients with type C0 (393.8 minutes) (P = 0.038). The rates of posttransplant complications were higher in patients with type C2 (100%) than in patients with type C0 (42.9%) and C1 (73.3%) (P < 0.001). Conclusion: MRP2 expression pattern was altered in 82.9% after LT. The pattern of MRP2 alteration was associated with longer operation time and higher rates of post-LT complications.

• Animal cells and systems
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• v.1 no.2
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• pp.363-370
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• 1997

RNase MRP is a ribonucleoprotein complex with a site-specific endonuclease activity. Its original substrate for cleavage is the small mitochondrial RNA near the mitochondrial DNA replication origin, thus it was proposed to generate the primer for mtDNA replication. Recently, it has been shown to have another substrate in the nucleus, such as pre-S.8S ribosomal RNA in nucleolus. The gene for the RNA component of RNase MRP (MRP RNA) was found to be encoded by the nucleus genome, suggesting an interesting intracellular trafficking of MRP RNA to both mitochondria and nucleolus after transcription in nucleus. In this study, genomic DNA encoding MRP RNA was microinjected into the nucleus of Xenopus oocytes, to analyze promoter regions involved in the transcription. It showed that the proximal sequence element and TATA box are important for basal level transcription; octamer motif and Sp1 binding sites are for elevated level transcription. Most of Xenopus MRP RNA was exported out to the cytoplasm following transcription in the nucleus. Utilizing various hybrid constructs, export of MRP RNA was found to be regulated by the promoter and the 5' half of the coding region of the gene. Interestingly, the transcription in nucleus seems to be coupled to the export of MRP RNA to cytoplasm. Intracellular transport of injected MRP RNA can be easily visualized by whole-mount in situ hybridization following microinjection; it also shows possible intra-nuclear sites for transcription and export of MRP RNA.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10597-10601
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• 2015

Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is sufficient to reverse MDR has yet to be validated. This study demonstrated that RNAi-based knockdown of MRP1 reversed the increased efflux ability and MDR efficiently. Two different short haipin RNAs (shRNAs) targeting MRP1 were designed and inserted into pSilence-2.1-neo. The shRNA recombinant plasmids were transfected into cis-dichlorodiamineplatinum-resistant A549 lung (A549/DDP) cells, and then shRNA expressing cell clones were collected and maintained. Real time PCR and immunofluorescence staining for MRP1 revealed a high silent efficiency of these two shRNAs. Functionally, shRNA-expressing cells showed increased rhodamine 123 retention in A549/DDP cells, indicating reduced efflux ability of tumor cells in the absence of MRP1. Consistently, MRP1-silent cells exhibited decreased resistance to 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and DDP, suggesting reversal of MDR in these tumor cells. Specifically, MRP1 knockdown increased the DDP-induced apoptosis of A549/DDP cells by increased trapping of their cell cycling in the G2 stage. Taken together, this study demonstrated that RNAi-based silencing of MRP1 is sufficient to reverse MDR in tumor cells, shedding light on possible novel clinical treatment of cancers.

• Korean Management Science Review
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• v.5 no.2
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• pp.42-47
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• 1988

본 논문은 범용 시스템으로서의 MRP에 대한 대안으로서 SNS를 소개하고, 두 시스템에 대한 비교 분석을 목적으로 한다. 이를 위해서 2장에서 MRP시스템의 문제점을 분석하고 3장에서 SNS의 기본원리를 소개한 후 4장에서 두 시스템을 비교 분석하였다.

• Korean Management Science Review
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• v.3
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• pp.62-70
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• 1986

본 고의 제2장에서는 생산시스템의 기본기능연관과 유형 및 관리 측면에서의 분석방법에 대해 설명하고, 제3장에서는 Honeywell에서 개발된 MRP(Manufacturing Resource Planning)응용 종합 생산관리 패키지인 HDMS(Honeywell Distributed Manufacturing System)를 중심으로 최신 폐쇄경로 MRP(Closed Loop MRP)개념 및 서브시스템에 대해서 설명하기로 한다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.1
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• pp.62-69
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• 2009

Maillard reaction products (MRPs) were produced from aqueous solution of various sugars with defatted hydrolyzed soybean protein (DFHSP) with different temperatures and pressures. Physicochemical properties of MRPs were investigated; also, DPPH and hydroxyl radical scavenging activity and sensory properties were evaluated. MRPs from ribose and DFHSP had the highest reactivity with larger pH reduce, higher browning index increase and higher antioxidant activity than other MRPs from other sugars. The antioxidant activities were increased with increasing temperatures and pressures of reaction. The highest antioxidant activity and sensory preference were obtained from MRPs with ribose at $140^{\circ}C$ with 2.8 kg/$cm^2$ for 30 mins.

• Elastomers and Composites
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• v.45 no.2
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• pp.106-111
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• 2010

Magneto-rheological elastomers (MREs) were manufactured by incorporation of magnetic responsible powder (MRP) into natural rubber and silicone rubber. The optimum loading amounts of MRP was 30 vol.% and the natural rubber based MRE (NR-MRE) showed better mechanical property than that of silicone rubber based MRE (S-MRE). However, the modulus shift ratio caused by S-MRE, measured by Self-modified Electromagnet Applied Fast Fourier Transform Analyser (SEFFTA), was higher than that of NR-MRE. The modulus shift ratio caused by NR-MRE was 10%, while the modulus shift ratio caused by S-MRE was 35.7%. The modulus shift ratio could be improved by orienting the magnetic direction of MRP before crosslinking the MRE. The degree of orientation of MRP was analyzed using SEM.

• Korean Management Science Review
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• v.5 no.2
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• pp.57-70
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• 1988

This paper examines both MRP and JIT for reducing WIP(Work In Process). The MRP/JIT system is suitable for multi-model, small-production manufacturing comparies in Korea because of the short delivery distance and low risk of accident during delivery. The objective of this paper is to manage vendors effectively by informing supply points and quantity of parts of vendors with MRP and JIT.